• Journal of The Korean Society of Grassland and Forage Science
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• v.19 no.3
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• pp.189-196
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• 1999

This study was conducted to evaluate the dry matter yield, quality and utilization of nutrients of wild Korean lespedeza as a forage legume sources. Dry matter(DM) yield of stages, chemical composition and DM digestibility were observed, also DM intake, digestibility and utilization of nitrogen and energy by Korean native goats were determined. Experimental diets include three treatrnents ; mixture grass hay(GS), GS 80% + lespedeza(LA) 20% and GS 60% + LA 40%, respectively. The DM yield of Korean lespedeza(LA) with advancing the growing stage tended to significantly increase(P<0.05). The average DM yield was observed 7,336kg/ha. CP content of LA tended to decrease, but fiber content tended to increase at growing stage advanced(P<0.05). The tannin content of LA was highest in bud stage (54.5mg/g), but tended to decrease at growing stage advanced, lowest in seed ripe stage(26.8mg/g) (P<0.05). With increasing the level of LA supplementation, DM intake by Korean native goats slightly increased, but no differences were observed in all diets. The digestibility of DM, cellular constituents and NDF were slightly lower for GS 100% diets than LA 20% and 40% supplemented diets(P<0.05). Apparently digested N% was not detected significant differences in all diets, but retained N and biological value were higher for LA supplemented diets than for GS 100% diets (P<0.05). Utilization of DE and ME was not detected significant differences in all diets. Above the results, it could be suggested that LA have a potential to provide as forage legume sources considering the DM yield, DM digestibility and nitrogen utilization.

• Journal of the Korean Society of Food Science and Nutrition
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• v.25 no.6
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• pp.1006-1015
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• 1996

This study was carried out to investigate the effects of dietary calcium-salt, estrogen-treatment, and estrogen/calcium treatment on calcium, phosphorus and nitrogen metabolism. Female Sprague-Dawley rats with a body weight of 250~280g were underwent ovariectomy or sham-operation. The ovariectomized rats were divided into 9 different experimental groups including the saline-treated group, the estrogen-treated group, the high calcium salt-treated group, and the estrogen/calcium treated groups and fed for 6 weeks. Each group daily intake and excretion of calcium, phosphorus and nitrogen were measured and apparent digestibility and balance were also studied. The results were as follows: The excretion level of winn calcium was significantly higher the ovariectomized rats than the sham-operation group(p<0.01) and reduced with estrogen treatment but this difference was not statistically significant. Fecal loss of calcium was higher the ovariectomized rats than the sham-operation group(p<0.001). When the estrogen was treated, fecal loss was decreased and then apparent digestibility of calcium was increased. Calcium balance was significantly higher the high calcium treated groups than the control diet groups. The excretion level of urinary Phosphorus was higher the ovariectomized rats than sham-operation group, while these showed to be decreased in the calcium salt, the estrogen and the estrogen/calcium treated groups(p<0.01). Fecal loss of phosphrous was higher in the ovariectomized rats. When the estrogen was treated, the fecal loss was decreased in the avariectomized rat than that of other groups. The excretion level of urinary nitrogen was higher the ovariectomized rats than the sham-operation, while these showed to be decreased in the estrogen, the estrogen/calcium, and the estrogen gradually reduction/calcium intensification. Fecal loss of nitrogen was decreased in tile estrogen treated group. The results in this study showed that high calcium, estrogen/calcium and estrogen gradually reduction/calcium intensification in the ovariectomized rats enhanced calcium balance compared to the ovariectomized rats without calcium treatment, but little effects on the phosphorus and nitrogen balance.

• Journal of The Korean Society of Grassland and Forage Science
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• v.31 no.2
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• pp.191-200
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• 2011

Four ruminally cannulated Hanwoo steers (BW 600 ${\pm}$ 48.4 kg) fed whole crop rice silage (WRS) as forage were used to investigate the effects of its dietary on rumen fermentation and blood metabolites at different harvesting time such as milk, dough, yellow ripe and mature stages of WRS, respectively. Mean ruminal pH sampled at 0, 1, 2, 4, 6 and 8 h after the morning feeding was not significantly (p>0.05) different between the WRS by the harvesting time. In diurnal pattern in ruminal pH, the lowest pH for the mature stage treatment (Mature) appeared at 6 h after the feeding while it appeared at 2-4 h for the other treatments. This may indicate that retention time in the rumen of Hanwoo fed WRS harvested at the Mature increased. Although feeding WRS harvested at different stages did not significantly (p>0.05) affect ruminal ammonia N concentration, the ammonia N for WRS harvested at the milk stage (Milk) tended (p=0.11) to decrease compared with that for the yellow stage treatment (Yellow). In the blood metabolites analysis, BUN concentration for Yellow numerically decreased compared with that for Milk. This, taking lower concentration of ruminal ammonia N for Milk than the other treatments into account, clearly indicates that the BUN concentrations may not increase in proportion to ruminal ammonia N concentration according to ammonia N concentration entering into the lower gastric tracts. Lower (p=0.03) concentration of blood albumin for the dough stage treatment than that for Mature may indicate high protein synthesis in Hanwoo fed WRS at the dough stage, but further studies in terms of mechanism of nutrients distribution should be required. Present results indicate that different harvesting time did not affect rumen fermentation and blood metabolites in Hanwoo. Thus, based on the results of the present and the previous studies, utilizing WRS harvested at yellow stage could be recommendable for Hanwoo steers.

• Applied Biological Chemistry
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• v.19 no.3
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• pp.172-183
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• 1976

RNA, DNA and other phosphorus fractions were determined in the leaf and root of soybean plants different in phosphorus sensitivity grown in $NH_4-N,\;NO_3-N$ and urea medium. The phosphorus sensitive cultivars contained higher ASIP (acid soluble inorganic phosphorus) than the tolerant cultivars with all nitrogen sources. ASIP was highest in the urea treated plants and lowest in the nitrate treated plants. Total phosphorus content was mostly affected with increase in ASIP. When ASIP increased, acid solsuble organic phosphorus(ASOP), phospholipids (L-P), RNA-P, residual phosphorus (R-P) tended to increase, while DNA-P showed little change. The percent RNA-P or DNA-P of total phosphorus in the nitrate treated plant was twice that in the ammonium treated plant, which were also higher in tolerant cultivars regardless of nitrogen sources. The percent ASOP in total acid soluble phosphorus $(ASOP/ASP{\times}100)$ decreased as phosphorus sensitivity decreased. Indications are that phosphorus sensitivity depends on the relative sizes of phosphorus metabolic pools. Total dry matter yield was negatively correlated with total phosphorus (r=0.84 significant at 0.01P), ASIP (0.84 significant at 0.01P) and residual phosphorus (0.69 significant at 0.05P). ASOP showed positive correlation with L-P, RNA-P and DNA-P but negative with R-P. RNA-P was significanly correlated only with L-P (0.63 at P=0.01). There was significant interaction (0.01) among nitrogen sources, cultivars and phosphorus metabolic pools. Phosphorus sensitivity and ammonium toxicity appear to be same in view of energy metabolism, that is, the former inhibits the conversion of ATP to ADP (energy releasing) through phosphate potential while the latter inhibits ATP formation (energy storing).

• Korean Journal of Soil Science and Fertilizer
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• v.3 no.1
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• pp.49-54
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• 1970

In an effort of determine the metabolism and bio-synthesis of nitrogen, was studied at variance of souble nitrogens, free amino acids and total alcohol soluble amino acids during the growth of younger soybean plants, and saybean divides into cotyledon and embryonic organ (shoot and root system) in this experiment. 1. In the soluble nitrogen of soybean, ratio of increase and decrease in the amino acids of them was displayed the near phenomena both cotyledon and embryonic organ of soybean. But, in the 17th days after seed germination, that is the developmeatal stage of adult leaf of soybeans, was appear the maximum value. It has been suggested that the stage of first half period of growth as boundary the stage of adult leaf development which indicated clear morphologically, at the younger soybean plants, is the step that nitrogen assimilation. 2. It was investigated the amino acids of seventeen kinds in the seed state, but at the third days after seed germination, was investigated the amino acids of nineteen kinds. Ultimately, it appears the translocation from cotyledon to embryonic organ in the distribution of amino acid, the nineth day which differentiation begining day of embryonic organ, then after, it happen the variation of number of inspected amino acid in the cotyledon and embryonic organ but only the variance changes in the distribution and quantitative aspects. Especially, the most conspicuous fact was indicated the accumulation of Asparagine, that is the phenomena of Asparagine-accumulation was constitute, not with standing no fertilization from outside. It may be concluded from the results of this investigation that the difference of special phenomena of soybean from the embryo of other plans. 3. In the initial stage of differentiation at embryonic organ number of inspected amino acid was very few, and then, it was slightly appeared the increase-phenomena in the number of them. It was that the amino acid inspecting the initial stage was translocated from the cotyledon. It is suggested that the intermediate-metabolism of amino acid was constituted on the basis of above the result. 4. The phenomena of increase and decrease of total alcohol soluble amino acid were essentially identical to the water soluble amino acid of soybean, but it was appeared the severe difference of amounts in both of them.

• Korean Journal of Microbiology
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• v.53 no.4
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• pp.337-339
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• 2017

Here we report the draft genome sequence of the Caballeronia sordidicola strain PAMC 26633, isolated from Psoroma species, a lichen material from Barton Peninsula, King George Island in Antarctica. As we have observed in previous genomic studies in the genus Caballeronia from polar lichen, draft genomic sequences of PAMC 26633 had an assortment of genes of ecological importance and of biotechnical potentials, which include diverse metabolic genes for carbohydrates, amino acids, and genes for nitrogen/sulfur metabolisms, stress responses, membrane transporters, antibiotic resistance, and heavy metal resistance. CRISPR genes and sequences were not found and there were some phage remnants and transposons.

• Journal of Animal Science and Technology
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• v.48 no.4
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• pp.541-554
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• 2006

Present review is to introduce an omasal sampling technique in rumen proteolysis and to consider some information on the omasal sampling technique with particular emphasis on methodological aspects. Use of the omasal sampling technique provides a new opportunity for accurate estimation of rumen metabolism with overcoming limitations of previous in vivo, in vitro and/or in situ methods. The potential advantages of the present technique compared with post-ruminal sampling techniques include following points; 1) only rumen cannulated animals are required, 2) less endogenous nitrogen (N) is contaminated in omasal digesta and 3) omasal digesta are devoid of exposure to acid peptide hydrolysis occurring in the abomasum. Estimates of soluble non-ammonia N (SNAN) in omasal digesta indicate that the assumptions underlying the in situ method that rapidly degradable N fraction can be degraded at an infinite rate and only insoluble dietary N escapes the rumen may be not valid. Quatitatively higher peptide concentration rather than free amino acid and soluble protein in escapable SNAN suggests that hydrolysis of peptide to amino acid may be the rate-limiting step in rumen proteolysis.

• Korean Journal of Soil Science and Fertilizer
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• v.33 no.5
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• pp.325-332
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• 2000

To determine the distribution and recovery of fall-applied N in various parts of satsuma mandarins (Citrus unshiu Marc). $19.68g\;N\;tree^{-1}$ as urea containing 5 atom % $^{15}N$ and 58 kg$K_2O$ $ha^{-1}$ were broadcast-applied to 11 years old 'Miyagawa Wase' grown at a spacing of $2.7{\times}2.7m$ on 18 November 1998. Nitrogen, $P_2O_5$, and $K_2O$ were applied at 104, 308, and $62kg\;ha^{-1}$ on 22 March and N and $K_2O$ at 42 and $83kg\;ha^{-1}$ on 15 June 1999. Two trees were excavated on 15 June and 8 December 1999, respectively. In mid-June, whole tree contained $168.2g\;N\;tree^{-1}$ of which 11.9, 42.1, 29.7, and 16.3% were in fruits, leaves, stems, and roots, respectively. In early December, total tree N averaged $169.8g\;tree^{-1}$ and fruits accounted for 27.6%, leaves 36.4%, stems 22.8%, and roots 13.2% of total tree N. Regardless of harvest date, N derived from fertilizer was highest in newly developed tissues. In mid June, the tree recovered 18.5% ($3.63g\;tree^{-1}$) of fertilizer N. Fruits accounted for 21.1%, leaves for 50.4%, stems for 21.5%, and roots for 7.9% of fertilizer-derived N in the tree, respectively. In early December, the tree recovered 17.0% of fertilizer N. Fruits contained 39.6%, leaves 40.5%, stems 14.5%, and roots 5.3% of fertilizer-derived N in the tree respectively. Comparing with total tree N, a higher proportion of fertilizer-derived N was allocated to metabolically active tissues while a less proportion to old tissues regardless of harvest date.

• The Korean Journal of Physiology
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• v.9 no.1
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• pp.23-32
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• 1975

In order to study the dally metabolism of sodium chloride and of nitrogen, 24-hour urine samples were collected from 1,593 normal Korean females whose ages varied from 2 to 80 years old. The volume, the concentration of chloride and the osmolality of the urine, add the total nitrogen were determined, along with the resting pulse rate and the blood pressure. The daily urine volume was maintained at $1,000{\sim}1,300\;ml/m^2/day$ in all age groups while the chloride concentration and osmolality of the urine samples were approximately 200 mEq/liter and 600 milliosmoles, respectively, in most of age groups. Hence the daily urinary output of sodium chloride was estimated to be approximately $15g/m^2$/day in adult groups. On the other hand, the daily excretion of total nitrogen amouted to approximately $5{\sim}6g/m^2/day$. These findings indicated that the average Korean females live on low-protein and high-salt diets throughout their life. Despite a known correlation between the incidence of hypertension and the high salt intake, none of the subjects employed in this work showed any sign of hypertension.

• Microbiology and Biotechnology Letters
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• v.1 no.1
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• pp.3-11
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• 1973

The biochemical characteristics of Astradix -P, isolated from Astragalus membranaceus Bunge as yeaststatic substance, were reported on a previous paper. And on this report, some relation to the nitrogen metabolism of yeast was studied. Inorganic or organic source of nitrogen easily uptaking yeast did not show any antagonistic action to the inhibitory action of Astradix -P on the yeast growth. Especially an organic nitrogen source, arginine, histidine and lysine, classified to basic amino acid, was reacted as an antagonistic substance to the sample. But, ornithine, a basic amino acid, did not show any antagonistic action to the sample. In the mixed media containing neutral and acidic amino acids as a nitrogen source, yeast growth was inhibited strongly. If the basic amino acid was added to the same mixed media, the yeast growth was not inhibited by Astradix-P therefore, the antagonistic action of basic amino acid to the Astradix-p was readily observed. The yeast static action of Astradix-P was partially related to the isoelectric point of amino acid as a nitrogen source. Yeast cells which propagated under the media containing growth inhibitor, Astradix -p, did not bring any remarkable denaturation of cell structure by electro-microscopic observation.