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Development of Direct Competitive Enzyme-Linked Immunosorbent Assay using Monoclonal Antibody (MAb) against Sulfamthazine (SMZ) and Establishment of Application Condition for Milk Sample (설파메타진에 단클론성 항체를 이용한 직접경쟁효소면역분석법의 개발과 우유 시료 적용 조건 확립)

  • Shim, Won-Bo;Mun, Chun-Sun;Kim, Jung-Sook;Choe, Ju-Mi;Kim, Ji-Hun;Park, Seon-Ja;Kang, Sung-Jo;Chung, Duck-Hwa
    • Korean Journal of Food Science and Technology
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    • v.38 no.2
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    • pp.176-182
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    • 2006
  • Sensitive and specific monoclonal antibody (MAb) was produced from hybridoma (1H11-5) obtained by fusion of myeloma cell (V653) and spleen cell isolated from mouse immunized sulfamthazine (SMZ)-HG-KLH. Direct competitive ELISA was developed for rapid detection of SMZ in milk samples using MAb against SMZ with optimized conditions between MAb and SMZ-HG-HRP conjugate, and applicable conditions for analysis of milk samples were established. Detection range of immunoassay was 0.1 to 100 ppb. Recoveries from spiked raw milk and processed milk samples averaged 82.1-120.7 and 82.1-97.1%, respectively.

Effects of Nitrogen Application on the Patterns of Amino Acids, Nitrogen Contents and Growth Response of Four Legume Plants under Saline Conditions (염분 환경하에서 4종 콩과식물의 생장, 아미노산 및 질소함량에 미치는 질소원의 영향)

  • 배정진;추연식;김진아;노광수;송종석;송승달
    • The Korean Journal of Ecology
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    • v.26 no.3
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    • pp.135-142
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    • 2003
  • Four legume plants showed better growth by the external nitrogen supply rather than the symbiotic nitrogen fixation only under salt additions. In case of Glycine max and Phaseolus angularis, total nitrogen contents decreased by high salinity level but their amino acid levels significantly increased with the increase of salt treatments and indicated high soluble-/insoluble-N ratios. Cassia tora and Albizzia julibrissin contained less amino acids than G. max and P. angularis but total N (esp. insoluble N fraction) increased with higher salt levels. Asparagine occurred as a main amino acid especially in G. max and P. angularis and can be seen as potential N-storage form in these plants. It might be play an important role for the osmoregulation mechanism under the saline condition. Meanwhile, to investigate what kinds of nitrogen sources are effective for overcoming salt stress on soybean plants, various N forms and concentrations (NH₄NO₃-N, NO₃-N, NH₄NO₃-N; 2.5 and 5 mM) were additionally supplied to the salt gradient medium. Soybean plants treated with NH₄NO₃-N showed the best growth up to 40 mM NaCl and NO₃- fed plants indicated good growth even at 80 mM NaCl treatments. Contrary to NH₄NO₃- and NO₃- fed plants, NH₄/sup +/- fed plants showed remarkable growth reduction and died by 40 and 80 mM NaCl treatments after the first harvest (15th day). Consequently, these results suggest that salt excluding and resistant capacities of soybean plants under NaCl treatments are increased in order of NH₄ - N, control, NO₃- N and NH₄NO₃- N depending on N concentration except NH₄- N treatments.

Deduction of affecting factors for selecting the way of going into the main building of an apartment complex when constructing an underground parking lot. (지하주차장 공사시 주동진입방법 선정 영향요인 도출)

  • Song, Nak-Hyun;Whang, Kyoung-Jin;Lee, Chan-Sik
    • Proceedings of the Korean Institute Of Construction Engineering and Management
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    • 2007.11a
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    • pp.995-998
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    • 2007
  • The number of households old over as much as 20 years is expected to get increased rapidly more than 1,000,000(1 million). As a measure to this fact remodeling is being revitalized over the country. parking lots expansion actually takes up a great deal of weight among the requirement for remodeling. When constructing a parking lot for enlargement, the way of going into the main building determines such construction features as selection of Retaining Wall Method, installation of an elevator, parking measure of capacity, etc. This study derived the affecting factors for selecting the way of going into the main building when constructing an underground parking lot. In a research way, we examined the features on each method of going into the main building in an apartment complex through an in-depth interview with some experts after investigating the method of enlarging an underground parking lot and going into the main building. After that, we derived the affecting factors for selecting the way of going into the main building. The affecting factors include constructability, convenience, economic efficiency as a high level as well as existence and nonexistence of basement and removal, safety of current building, parking measure of capacity, amenity, inhabitant traffic line, construction expenses and construction duration as a low one. This study will be applied as a reference for selecting the way of going into the main building when constructing an underground parking lot especially targetted on an old apartment house.

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An Instructional Model for the Improvement of Decision Making Skills in the 'Technological Innovation and Invention' Unit of Technology·Home Economics Curriculum (고등학교 기술·가정과 '기술 혁신과 발명' 단원에서 의사결정능력 향상을 위한 수업 모형 개발)

  • Seo, Joung-Wan;Lee, Yong-jin
    • 대한공업교육학회지
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    • v.41 no.1
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    • pp.108-127
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    • 2016
  • We confront incessant choices in life. A person or a group stands at a crossroad continuously and make decisions under limited conditions in each time in order to achieve a goal. These series of processions are referred to as decision making and dominates our actions. Based on this view, the decision making is crucial and it secures its own position in school education as well as in real life. The purpose of this study is to develop decision making skills instructional model that can be applied to improve decision making skills in technological subjects. The components of decision making skills in technological education was problem recognition, problem identification, alternative production, alternative assessment, optimum selection, optimum evaluate. An instructional models for improvement of decision making skills in technology education and examination was designed according to the components of decision making skills in technological education. Depending on the components of decision making skills developed a teaching material to experience each step. And in the teaching material was to verify the improved decision-making skill. Accordingly, a decision making instruction model in technology is expected to contribute to enhance students' decision making skills.

A Microscope Study of Fluoride Effects on the Rat Incisor Enamel Formation (흰쥐 절치의 법랑질 형성에 미치는 불소의 효과에 관한 현미경적 연구)

  • Lim, Do-Seon;Chang, Byung-Soo;Jeong, Je-O;Jeong, Soon-Jeong;Jeong, Moon-Jin
    • Applied Microscopy
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    • v.34 no.2
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    • pp.145-157
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    • 2004
  • The aim of the present study was to examine in detail, both at light and electron microscopical levels, the morphological variations in ameloblast of the fetal rat incisor enamel organ. Rats were started on distilled water at the beginning of pregnancy. The pups were sacrificed 11 days after delivery and animals were perfused intravascularly with glutaraldehyde and the incisors were removed. To examine on the ultrastructure of the ameloblast, the study employed primary light microscopy but electron microscopy was used to clarify some of the light microscopic finding. Longitudinal sections through the incisors of the rat show a continuous layer of ameloblasts on the labial surface of the tooth. This layer contains the entire sequence of developmental stages in enamel production. The ameloblast layer was divided into three main zones: 1) Presecretory zone, region of ameloblasts facing pulp. 2) Secretory zone, region of inner and outer enamel secretion. 3) Maturation zone, region of reduced ameloblasts. In particularly, the present study has shown that two distinctively different types of ameloblasts appear in the enamel organ during enamel maturation in the rat incisor. These two types have been designated ruffle-ended ameloblasts (rAB) and smooth-ended ameloblasts (sAB). The fluoride produces marked alteration in the fine structure of ameloblast from teeth of young rats, such as large confluent distensions of the endoplasmic reticulum and swelling of isolated mitochondria. This experimental data suggested that exposure prolonged of animal to high level of fluoride appears to induce a few dramatic changes in the normal appositional growth and initial mineralization of enamel created during amelogenesis.

A Comparative Study of Mathematics Curriculum among the United States, Singapore, England, Japan, Australia and Korea (수학 교육과정 국제 비교 분석 연구 - 미국, 싱가포르, 영국, 일본, 호주의 중학교와 고등학교 교육과정을 중심으로 -)

  • Chong, Yeong Ok;Chang, Kyung-Yoon;Kim, Gooyeon;Kwon, Na Young;Kim, Jin Ho;Seo, Dong-Yeop;Kang, Hyun-Young;Park, Sunhwa;Ko, Ho Kyoung;Nam, Jin Young;Tak, Byungjoo
    • Journal of Educational Research in Mathematics
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    • v.26 no.3
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    • pp.371-402
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    • 2016
  • The aim of this study is to compare mathematics curriculum among the United States, Singapore, England, Japan, Australia and Korea and offer suggestions to improve mathematics curriculum of Korea in the future. In order to attain these purposes, the analysis was conducted in many aspects including mathematics education system, mathematics courses, mathematics contents, assessment syllabus for university entrance examination and the construction principles of mathematics curriculum. In the light of the results of this study, our suggestions for improving mathematics curriculum of Korea are as follows: revising the contents of analysis, geometry, probability and statistics strands; organizing curriculum based on spiral construction principle; providing various opportunities to select mathematics courses according to students'career; reflecting the contents of their courses in university entrance examination.

Simulating Carbon Storage Dynamics of Trees on the Artificial Ground (시뮬레이션을 통한 인공지반 교목의 탄소저장량 변화)

  • You, Soo-Jin;Song, Ki-Hwan;Park, Samuel;Kim, Se-Young;Chon, Jin-Hyung
    • Journal of the Korean Institute of Landscape Architecture
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    • v.45 no.2
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    • pp.11-22
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    • 2017
  • To successfully create a low-carbon landscape in order to become a low-carbon city, it is necessary to understand the dynamics of artificial greening's resources on a multi-scale. Additionally, the effects of carbon storage should be quantitatively evaluated. The purpose of this study is to simulate and evaluate the changes in carbon storages of artificial ground trees using system dynamics throughout a long-term period. The process consisted of analyzing the dynamics of the multi-scale carbon cycle by using a casual loop diagram as well as simulating carbon storage changes in the green roof of the Gangnam-gu office building in 2008, 2018, 2028, and 2038. Results of the study are as follows. First, the causal loop diagram representing the relationship between the carbon storage of the artificial ground trees and the urban carbon cycle demonstrates that the carbon storage of the trees possess mutual cross-scale dynamics. Second, the main variables for the simulation model collected 'Biomass,' 'Carbon storage,' 'Dead organic matter,' and 'Carbon absorption,'and validated a high coefficient of determination, the value being ($R^2$=0.725, p<0.05). Third, as a result of the simulation model, we found that the variation in ranking of tree species was changing over time. This study also suggested the specific species of tree-such as Acer palmatum var. amoenum, Pinus densiflora, and Betula platyphylla-are used to improve the carbon storage in the green roof of the Gangnam-gu office building. This study can help contribute to developing quantitative and scientific criteria when designing, managing, and developing programs on low-carbon landscapes.

The Construction of Multiform User Profiles Based on Transaction for Effective Recommendation and Segmentation (효과적인 추천과 세분화를 위한 트랜잭션 기반 여러 형태 사용자 프로파일의 구축)

  • Koh, Jae-Jin;An, Hyoung-Keun
    • The KIPS Transactions:PartD
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    • v.13D no.5 s.108
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    • pp.661-670
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    • 2006
  • With the development of e-Commerce and the proliferation of easily accessible information, information filtering systems such as recommender and SDI systems have become popular to prune large information spaces so that users are directed toward those items that best meet their needs and preferences. Until now, many information filtering methods have been proposed to support filtering systems. XML is emerging as a new standard for information. Recently, filtering systems need new approaches in dealing with XML documents. So, in this paper our system suggests a method to create multiform user profiles with XML's ability to represent structure. This system consists of two parts; one is an administrator profile definition part that an administrator defines to analyze users purchase pattern before a transaction such as purchase happens directly. an other is a user profile creation part module which is applied by the defined profile. Administrator profiles are made from DTD information and it is supposed to point the specific part of a document conforming to the DTD. Proposed system builds user's profile more accurately to get adaptability for user's behavior of buying and provide useful product information without inefficient searching based on such user's profile.

Development of Wide-Band Compensation Film to Improve Viewing Angle of Vertical Alignment Liquid Crystal Display (수직배향 액정디스플레이의 시야각 향상을 위한 광대역 보상필름 개발)

  • Choi, Yu-Jin;Lim, Young-Jin;Jeong, Kwang-Un;Lee, Seung-Hee
    • Polymer(Korea)
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    • v.35 no.4
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    • pp.337-341
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    • 2011
  • To expand the viewing angle of vertical alignment liquid crystal display (VA-LCD), a wide-band compensation film has been newly developed. VA-LCD needs a retardation film with improved oblique viewing property. The retardation film for VA-LCD has been fabricated by solvent casting and subsequent drawing triacetylcellulose (TAC) containing optical additives. Here, optical birefringence and wavelength dispersion of the retardation film strongly depend on the chemical structures of optical additives. Utilizing quantum mechanical computation, the polarizing anisotropy of retardation film was calculated with respect to additives. It is also realized that the wavelength dispersion of polarizing anisotropy depends on the type and location of substituent to the selected main isomer. When two propionates are substituted to the meta position of the selected main isomer, the wavelength dispersion of polarizing anisotropy shows the most gentle slop, which is well matched with the experimental results. The most gentle slop of wavelength dispersion means that the viewing angle characteristics of LCD are less influenced according to the wavelength of light source of LCD, and it's possible to make better image quality than the present level.

Measuring of Gender Inequality: Asymmetry of Marriage Table with respect to Educational Level (교육수준 별 혼인표의 비대칭성으로 살펴본 남녀불평등지수)

  • 이명진
    • Korea journal of population studies
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    • v.25 no.1
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    • pp.33-50
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    • 2002
  • This study examines cross-national patterns of asymmetry of marriage tables with respect to educational level and tries to measure the degree of gender inequality across nations. A Primary assumption of the study is that gender inequality inhibits symmetric marriage between men and women. As men and women differ more in status, the rate of symmetric marriage between them declines thus producing asymmetric marriage with respect to social status. More specifically, the main object of the study is to develop statistical models and index with which to assess the patterns and degree of asymmetric marriage. Additionally, it is intended to assess the appropriateness of several theoretical perspectives for explaining these variations identified by the statistical models. Two most important such perspectives are industrialism and theory of politics and culture. To answer these questions, this study relies on twenty-seven marriage tables with respect to educational level, some from published tables, and some extracted from other sources. The main findings of the study are: (1) compared to less industrialized countries, more industrialized countries have lower degrees of asymmetric marriage(gender inequality) with respect to educational level, and (2) other things being equal, differences in politics and culture seem to have the some impact on marriage pattern; for instance, social democracy and state socialism reduce the degree of asymmetric marriage while the high emphasis on gender-based hierarchy in Asian countries seems to increase it In short, these results suggest a weaker or modified version of industrialists That is, while with economic growth most nations show a decline in the degrees of asymmetric marriage with respect to social status, for some nations the degrees of asymmetric marriage are affected by their specific politics or cultures.