• Title/Summary/Keyword: 진균분리빈도

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Mycological Studies of Superficial Fungal Disease : Pathogenic Fungus and Prevalence (피부진균증의 원인진균과 발현 빈도에 관한 연구(I))

  • Shin, Hyun-Sung
    • The Korean Journal of Mycology
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    • v.29 no.1
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    • pp.28-33
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    • 2001
  • This study was done with 105 cases of dermatomycosis of dermatological patients who had visited the dermatology clinic in Taejon city. Of dermatophytosis, the decreasing orders of clinical location by the incidence were Tinea pedis (44.8%), Tinea cruris (30.5 %) and Tinea corporis (10.5%). The organisms identified from dermatophytoses were 6 genus: the decreasing orders of frequency were Trichophyton spp. (43.5%), Candida spp. (12.9%), Microsporium spp. (3.2%) and Epdermophyton flocosum(1.6%). The most common causative organisms was Trichophyton spp.

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Study on Distribution of Yeast Isolated from Clinical Specimens for Six Years in a University-affiliated Hospital (일개 대학병원의 임상검체에서 분리된 6년간의 효모균 분포에 관한 연구)

  • Ma, Pan-Gon;Kim, Sun-Joo;Seo, Choong-Won;Yu, Young-Bin;Kim, Young-Kwon
    • Journal of Digital Convergence
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    • v.13 no.9
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    • pp.369-375
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    • 2015
  • We investigated the prevalence of fungi isolated from a university-affiliated hospital during 6 years (2006-2011) to provide relevent information for the patient management. The general characteristics of the clinical isolates and gender, age, and type of specimens were analyzed. Among a total of 163,530 requested samples to culture for the Laboratory of Clinical Microbiology, Department of Laboratory Medicine, Gyeongsang National University Hospital in the Republic of Korea, 5,387 (3.3%) showd positive results for fungi. The most prevalent isolates were Candida albicans 41.9%, Candida glabrata 15.5%, and Candida tropicalis 14.6%. Total isolates of fungi increased from 526 in 2006 to 1,145 in 2011. They were most commonly isolated from sixties (27.0%) and seventies (26.5%). The most common clinical specimen was urine (44.8%). Males (52.4%) were slightly more than females (47.6%). In the future, a nationwide survey and additional antifungal convergence drugs susceptibility results will provide more useful information.

Isolation and Identification of Entomopathogenic Fungi Collected from Mountains and Islands in Korea (한국의 산과 섬 지역에서 채집한 곤충기생 진균의 분리 및 동정)

  • Kim, Wan-Gyu;Seok, Soon-Ja;Weon, Hang-Yun;Lee, Kang-Hyo;Lee, Chan-Jung;Kim, Yang-Sup
    • The Korean Journal of Mycology
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    • v.38 no.2
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    • pp.99-104
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    • 2010
  • Dead insects infected with fungi were collected from mountains and islands in several locations in Korea from 2003 to 2007. Morphological characteristics of the fungal fruiting bodies produced on the insect samples were examined. A total of 542 entomopathogenic fungal isolates were obtained from the insect samples and identified based on their morphological and cultural characteristics. Three species of Beauveria, eight species of Cordyceps and four species of Isaria were mainly isolated from the insect samples. The other entomopathogenic fungi isolated from the insect samples were Metarhizium anisopliae, Nomuraea rileyi, Paecilomyces sp. and Verticillium sp.

Distribution of Hospital Airborne Microorganisms in Seoul, Korea (서울시내 종합 병원 공기중의 미생물 분포)

  • Hong, Jun-Bai;Chung, Yun-Hee;Yun hee Chang
    • Journal of Environmental Health Sciences
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    • v.29 no.1
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    • pp.1-7
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    • 2003
  • This study was carried out to investigate the airborne microbial pollution in hospital environment. Using a mechanical air sampler, microbiological samples were taken from intensive care unit, general ward room, patients wailing room and outdoor of 20 hospitals in Seoul, Korea. The concentration of airborne bacteria and fungi ranged 97-410 cfu/㎥ and 37-77 cfu/㎥, respectively and patients waiting room had highest bacterial count. 10 genera of molds were identified and the most frequently recovered molds were Aspergillus, followed by Penicillium, Alternaria and Cladosporium. Among Staphylococcus species, S. haemolyticus and S. epidermidis were predominant and 47% of Staphylococcus species were isolated from intensive rare unit.

Distribution of Yeast Isolated from Clinical Specimens at a University Hospital in last Five Years (최근 5년간 단일 대학병원의 임상검체에서 분리된 효모균의 분포)

  • Seo, Choong-Won;Yu, Young-Bin;Shin, Kyeong Seob;Kim, Young-Kwon
    • Journal of Digital Convergence
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    • v.12 no.9
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    • pp.237-244
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    • 2014
  • Pathogenic fungal infections are predominantly occurred in patients with severe immune or metabolic defects. In the present study, we aimed to investigate the last five years (2007~2011) 190,250 cases clinical specimens of yeast 3,487 results that had shown positive culture and to look at the significance of regional difference and identify relationship between provide the characteristics about association between clinical isolates and gender, age, and type of specimens. The yearly strain-specific isolation frequency of yeast separated was 1,925(55.2%) for C. albicans the largest of them. All kinds of clinical specimen was 1,495(42.9%) in urine, 998(28.6%) in sputum. Strain-specific gender differences in C. albicans for males was 1,177(57.8%) of the total of 2,037 and 748(51.6%) of 1,450 and as for age, those between 70 and 79 were the largest with 639(55.1%) of the 1,925 strain. In this study, well presented the general characteristics of pathogenic yeast seen in diverse specimens. This limitation has been implemented in a single area, Future research is expected to examine more on nationwide distribution chart, dynamic characteristics and future antibiotic sensitivity.

Analysis of Fungal Concentration and Species Present as Bio-aerosols in Oak Mushroom Cultivation Houses (국내 표고버섯 재배사에 바이오에어로졸로서 분포하는 진균의 농도와 종 분석)

  • Kim, Seong Hwan;Kim, Ji Eun;Kim, Jun Young
    • The Korean Journal of Mycology
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    • v.46 no.4
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    • pp.393-403
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    • 2018
  • Bio-aerosols transported by the air have been considered as the major source of dispersal agents that contaminate agricultural products. Unseen fungal spores are known sources of bio-aerosols that harm mushroom and human health during mushroom cultivation. This study was conducted to obtain basic data on the concentration and species distribution of fungi present in the indoor air of oak mushroom cultivation houses in Korea. In 2015 and 2016, we sampled and analyzed indoor airborne fungal spores 21 times from 13 oak mushroom cultivation farms located in six different provinces. The concentration of airborne fungi ranged from $1.30{\times}10^2$ to $1.59{\times}10^4cfu/m^3$. Surprisingly, in 20 sampling cases, the fungal concentration exceeded $500cfu/m^3$, which is recommended as the indoor air quality standard by the Ministry of Environment, Korea. A total of 450 fungi were isolated and identified to belong to 33 genera and 46 species. Among the identified fungi, human pathogens (4 genera and 4 species) and plant pathogens (10 genera and 13 species) were present. In addition, Trichoderma harzianum, Trichoderma atroviride, and Trichoderma longibrachiatum, which are detrimental species that affect mushroom health, were found 17 out of 21 sampling times. Our results provide evidence that indoor air quality should be improved for better management of mushroom cultivation houses.

Air-borne Fungi in the Air of Seoul (서울 지방의 공중(空中) 진균(眞菌)에 관한 연구(硏究))

  • Min, Kyung-Hee;Lee, Yung-Ja
    • The Korean Journal of Mycology
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    • v.13 no.2
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    • pp.115-121
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    • 1985
  • An aerial survey for fungal flora in Seoul has been carried out by Petri plate exposure method for a period of one year. A total of 2227 fungal spores appeared in the plates. Of these, Cladosporium was the commonest fungus representing 43.5% of the total spore count followed by Penicillium (15.8%). Alternaria (10.5%), and Aspergillus (3.8%). There were seasonal variations in the prevalence of fungal spores. The occurrence of these fungi was greatly affected by climatic conditions. However, Alternaria appeared to prefer warmer weather. A comparison of fungal population from the outdoor and indoor air was also investigated to support the view that Alternaria is abundant population from the outdoor air and that Cladosporium from the indoor air depending on their availability in the atmosphere.

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Occurrence and control of postharvest diseases of apple (사과저장병의 발생 및 방제)

  • Kim, Yong-Ki;Kim, Ryung-Hee;Ryu, Jae-Dang;Ryu, Jae-Gee;Lee, Sang-Yup;Choi, Yong-Chul
    • The Korean Journal of Pesticide Science
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    • v.2 no.2
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    • pp.83-89
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    • 1998
  • The occurrence of postharvest disease of apple was surveyed from 1995 to 1997 in the major apple-producing area in Korea. Ten genera including Alternaria spp., Botryosphaeria dothidea, Botrytis cinerea, Fusarium spp., and Penicillium spp. were isolated from the decayed apple fruits. Of these, B. cinerea, Penicillium spp. and Fusarium spp. were frequently isolated and were highly pathogenic to apple fruits. Optimum temperature of mycelial growth for Penicillium spp. ranged from 10 to $30^{\circ}C$ and that of mycelial growth for B. cinerea and Alternaria spp. ranged from 5 to $30^{\circ}C$. Optimum temperature of sporulation of Penicillium spp. and Alternaria spp. ranged $15{\sim}25^{\circ}C$ and $10{\sim}20^{\circ}C$, respectively. Occurrence of postharvest disease of apple increased in neglecting selection of wounded fruits before storing apples. Most of these fungi causing postharvest diseases such as Penicillium spp, Botrytis cinerea and Alternaria spp. were isolated from healthy fruits sampled at apple orchard. These results suggested that posthavest diseases of apple were originated from apple fruits contaminated from apple orchard and occurred during storage. In addition, five fungicides including prochloraz EC, fenari EC, captan WP, benomyl WP and folpet WP suppressed posthavest diseases when they sprayed on apple fruits at 30 days before harvesting.

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Clinical Analysis of Polymicrobial Bloodstream Infections in Pediatric Patients: Epidemiology, Clinical Features, Organisms, and Risk Factors (소아 환자에서 다균혈증에 대한 임상적 고찰)

  • Kim, Jung Min;Park, Hye Jin;Kim, Ki Hwan;Kim, Dong Soo
    • Pediatric Infection and Vaccine
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    • v.17 no.2
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    • pp.83-90
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    • 2010
  • Purpose : Although the incidence of polymicrobial bloodstream infection (PBSI) has increased, only a few studies have so far focused on children. Therefore, in an effort to prevent more serious situations in pediatric patients, we analyzed the clinical features, organisms, and laboratory results of PBSI. Methods : We performed a retrospective review of the case records of 97 patients with polymicrobial bloodstream infection in the Severance hospital, from 2001 to 2008. Using t-test and chi-square test, we analyzed the underlying medical conditions, clinical characteristics, organisms, and laboratory results of those patients. Results : Annual incidence of polymicrobial bloodstream infection increased from 1.4 % in 2001 to 10.9% in 2008 in pediatric patients. Immunocompromised hemato-oncological malignancy was found in 31 (31.9%) patients, and was the most common underlying medical condition; cardiovascular disease was found in 15 patients (15.4%), neurologic disease in 10 patients (10.3%), and so on. Gram positive organisms were recovered in 143 cases and gram negative organisms were recovered in 101 cases of PBSI. Staphylococcus epidermidis was the most common organism. Factors affecting mortality included underlying medical disease, immune status, nosocomial infection, and central catheter-related infection, for which the rate of mortality showed a greater increase (P<0.05). Conclusion : Due to the close connection between PBSI and fatal conditions or high mortality, it requires more aggressive management. Compared with previous studies, we discovered that immunocompromised hemato-oncological malignancy was the most common underlying medical condition and that frequency of gram-positive bacteria and fungus isolated has increased.

Isolation and Characterization of Endophytic Bacteria from Rice Root Cultivated in Korea (한반도 중부지방의 벼 뿌리로부터 내생 세균의 분리와 특성 분석)

  • Park, Soo-Young;Yang, Sung-Hyun;Choi, Soo-Keun;Kim, Ji-Hyeon;Kim, Jong-Guk;Park, Seung-Hwan
    • Microbiology and Biotechnology Letters
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    • v.35 no.1
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    • pp.1-10
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    • 2007
  • The 44 endophytic bacterial strains were isolated from surface-sterilized root of rice cultivated in seven different locations of Chungcheong province, Korea. Each isolate was introduced into rice seedlings grown gnotobiotically by inoculating scissor-cut first true leaf with cell suspensions, and the colonization capacity of each isolate in root tissue was analyzed at 7 days after inoculation. Sixteen out of 44 isolates were re-isolated from root successfully with the frequency of $10^{3-5}$ CFU/g tissue. Interestingly, seven out of 16 isolates were identified as Burkholderia species. The identity between inoculated strains and re-isolates was confirmed by genomic finger-printing and 16S rDNA sequence analysis. By a confocal laser scanning microscopic observation it was revealed that KJ001 strain, one of the sixteen isolates tagged with gfp colonized in root tissue especially around xylem. Six out of seven Burkholderia strains obtained in this study showed antagonizing activities against seven different fungal pathogens, contain nifH gene, and five of them enhanced growth of cucumber over 30%. The isolates showed no hypersensitive response on tobacco leaves and no pathogenecity in rice. From these results it was found that the endophytic Burkholderia strains will be useful in agriculture to develop a biocontrol agent or a bio-fertilizer.