• Proceedings of the Korean Institute of Intelligent Systems Conference
• /
• 2004.04a
• /
• pp.93-96
• /
• 2004

생태학, 환경공학, 임상진단 둥 여러 생물학 분야에서 미생물의 다양성 연구의 중요성이 대두되고 그 연구가 점증하고 있다. 특히 16S rRNA를 분자지표로한 DNA 염기서열 분석방법이 널리 사용되고 있다. 본 논문에서는 16S rRNA의 염기서열 분석과정을 각 단계별로 자동화하고, 생물학자들의 결과 판단이나 사용상의 편의를 도모하기 위하여 웹기반의 미생물 다양성 분석 어플리케이션을 개발하였다. 개발을 위하여 단계별 자동화 및 인터페이스 개발에 적합한 폴더 프로세스-필터 모델을 고안하고 적용하였다. 제공되는 생물정보분석도구는 서열입력, 서열방향교정, 다중서열정렬 및 가시화, 서열동정 등의 분석등이 있으며, 각 결과는 계통분류도구와 호환가능하도록 하였다. 또한 신생아의 장내 세균총에 대한 분석을 수행하여 개발된 도구의 유용성을 확인하였다. 개발된 웹 에플리케이션은 리눅스 시스템 상에서 Perl 과 CGI를 이용하였으며, http: //home.pusan.ac.kr/~genome/tools/rat.htm으로 접속하여 사용할 수 있다.

• Journal of Korean Society of Environmental Engineers
• /
• v.32 no.2
• /
• pp.121-130
• /
• 2010

Metals and sulfate can be considerably dissolved at low pH condition in the acid mine drainage(AMD) and it would make an environmental problems. There are only few of acid mine drainage treatment systems in Korea which are operating, but these still have an effect on the surrounding stream. In this study, quantification of indicator microorganisms was conducted to judge the environmental impact of AMD on microflora by quantitative real-time PCR in the drainage samples of four mines and the water samples of each surrounding stream. Two species of iron reducing bacteria(Rhodoferax ferrireducens T118 and Acidiphilium cryptum JF-5) were selected for indicator bacteria based on 16S rRNA cloning analysis, and sulfate reducing bacteria(Desulfosporosinus orientus), iron and sulfur oxidizing bacteria(Acidothiobacillus ferrooxidans) and iron oxidizing bacteria(Leptosprillum ferrooxidans) were included into indicator since these were found in the previous studies on the mining area. Thereafter, the comparative analysis of four mines were established by the microbiological variation index and it was determined that the biological environment effect of AMD is highest in Samtan mine which doesn t contain treatment system by the value.

• Korean Journal of Soil Science and Fertilizer
• /
• v.31 no.3
• /
• pp.285-290
• /
• 1998

Thermophilic Bacillus species was studied as a microbial indicator to pursue the compost a application history. Thermophilic Bacillus species could be easily determined by the plate culture method within 12 hours in $65^{\circ}C$ incubator. The density of thermophilic Bacillus species in soils was gradually increased with the application rate of rice straw compost, and correlated to the soil organic matter content in $R^2=0.835^{**}$(n=32) coefficient on the 43-year-long term rice paddy fields.

• Microbiology and Biotechnology Letters
• /
• v.31 no.4
• /
• pp.413-420
• /
• 2003

To explore the effective treatment methods for waste food generating malodor compounds, mixed microorganisms (MM) capable of deodorizing of domestic animal waste were applied using household composting box. The mixture of 5 kg whole chips as a bulk agent, 2 kg MM and 1 kg of waste food were input into the compost reactor and agitated. Waste food was supplemented every 24 hours. As the results, the composting volume was stable at 13∼14 L for 10 days. In the initial compost process with MM, the pH and temperature were increased more quickly than that of without MM. Also, the conductivity recognized as a barometer of compost was increased from 0.2 to 2.4 mS/cm that was higher than 1.3 mS/cm of without MM, for 10 days. The malodor compounds generated from waste food treatment such as sulfur compounds and volatile fatty acids were effectively reduced about 90∼l00%, and 70∼80% for 8 days, respectively. The microorganisms growing under the condition of alkaline phase and higher temperature were dominated during the compost Moreover, it was demonstrated that inoculated Bacillus cereus HY15 dominated during the compost results in responsible to the effective treatment of waste food.

• Journal of Korean Society of Water and Wastewater
• /
• v.20 no.6
• /
• pp.885-892
• /
• 2006

In order to investigate whether or not Total Coliforms (T.C.) and Fecal Coliforms (F.C.) are compatible as indicator microorganisms of waterbome enteric viruses, a total of 192 surface water samples from 24 locations in Korea were tested for T.C., F.C., and human enteric viruses from July 2003 to January 2006. Altogether, the number of T.C. in each samples was ranged from $0{\sim}5.3{\times}10^4$ colony forming unit(CFU)/100mL, and the number of F.C. ranged from $0{\sim}5.0{\times}10^3CFU/100mL$ per sample. Thirty-three percent of the samples tested positive for human enteric viruses after the total culturable virus assay. The results of the statistical analysis showed that T.C. and F.C. had a significant correlation with turbidity and temperature, but the waterbome enteric viruses did not. When compared to the number of T.C. or F.C. per sample, the concentration of waterbome enteric viruses was not found to be correlated. In conclusion, it is suggested that T.C. and F.C. may not be sufficient microbial indicators of waterbome enteric viruses in the samples analyzed in this study. However, further research is needed to find other microbial indicators of waterbome enteric viruses and to develop more advanced and sensitive methods to detect waterborne enteric viruses.

• Korean Journal of Food Science and Technology
• /
• v.37 no.2
• /
• pp.287-293
• /
• 2005

Microbial contamination levels in commercial strawberry juices were examined for sanitary indication bacteria, such as aerobic plate count (APC), coliforms, and Escherchia coli, and pathogenic bacteria such as E. coli O157:H7, Salmonella spp., Staphylococcus aureus, and Listeria monocytogenes. APC and coliform count ranged 0-5.2 and $0-2.8\;log_{10}\;CFU/(mL,\;g,\;100cm^{2},\;hand)$, respectively, and 80% strawberry juices were contaminated with E. coli and S. aureus, detected at 19%, was found in employee's hands, strawberries, and strawberry juices, whereas E. coli O157:H7, Salmonella spp, and L. monocytogenes were not detected. These results will provide microbiological information for introduction of Hazard Analysis Critical Control Point (HACCP) system in juice shops.

• Microbiology and Biotechnology Letters
• /
• v.40 no.1
• /
• pp.30-38
• /
• 2012

We investigated the effects on soil microbial diversity and the growth promotion of red pepper resulting from inoculation with a microbial agent composed of Bacillus subtilis AH18, B. licheniformis K11 and Pseudomonas fluorescens 2112 in a red pepper farming field. Photosynthetic bacteria, Trichoderma spp., Azotobacter spp., Actinomycetes, nitrate oxidizing bacteria, nitrite oxidizing bacteria, nitrogen fixing bacteria, denitrifying bacteria, phosphate solubilizing bacteria, cellulase producing bacteria, and urease producing bacteria are all indicator microbes of healthy soil microbial diversity. The microbial diversity of the consortium microbial agent treated soil was seen to be 1.1 to 14 times greater than soils where other commercial agent treatments were used, the latter being the commercial agent AC-1, and chemical fertilizer. The yield of red pepper in the field with the treated consortium microbial agent was increased by more than 15% when compared to the other treatments. Overall, the microbial diversity of the red pepper farming field soil was improved by the consortium microbial agent, and the promotion of growth and subsequent yield of red pepper was higher than soils where the other treatments were utilized.

• Journal of Korean Society of Environmental Engineers
• /
• v.29 no.9
• /
• pp.1020-1026
• /
• 2007

The control of pathogenic microorganisms is important issue in human environments, especially in surface area. However, surface disinfection has not been fully researched. In this study, the surface disinfection under UV irradiation was performed to investigate the quantitative kinetics for Bacillus subtilis spore inactivation in several experimental conditions, such as light intensity, temperature and surface roughness. This study reports that UV light would apparently inactivates the microorganisms and the required IT value for 2 log (99%) Bacillus subtilis spore inactivation was found to be 14.5 $mJ/cm^2$ in plain surface, as predicted by the Delayed Chick-Watson model. When UV was irradiated, there were no significant temperature effects. However, the experimental result shows that the more increased IT values are required at larger surface roughness.

• Korean Journal of Soil Science and Fertilizer
• /
• v.42 no.2
• /
• pp.139-143
• /
• 2009

We examined the vertical distribution of specific microbial groups and the patterns of microbial community structure within the soil profile using phospholipid fatty acid (PLFA). Samples were collected from the soil surface down to 15 cm in depth from paddy and upland fields located in Daegu, Korea. The two fields have been fertilized with only chemical fertilizers N, P, K for 33 years. Principal component analysis of the PLFA signatures indicated that the composition of the soil microbial communities changed significantly with the cultivation practices and soil depth, suggesting that cultivation practices of paddy and upland fields had more significant influence on soil microbial community than the soil depth did. The soil microbial communities changed more drastically with soil depth in upland field than in paddy field, with making thicker soil surface in paddy field in terms of soil microbial community. The ratios of cyclopropyl/monoenoic precursors and total saturated/total monounsaturated fatty acids increased with soil depth, suggesting that the deeper soil horizons are more carbon-limited and anaerobic than surface soil. The community analysis using PLFAs as biomarkers revealed that Gram-positive bacteria and actinomycetes tended to increase in proportional abundance with increasing soil depth, while the abundance of Gram-negative bacteria and fungi were highest at the soil surface and substantially lower in the subsurface.

• Korean Journal of Soil Science and Fertilizer
• /
• v.43 no.6
• /
• pp.1012-1017
• /
• 2010

This study was carried out to investigate the effect of continuous cropping of pepper on soil microbial phospholipid fatty acids (PLFAs) under upland applied without any pesticides and chemical herbicides from 2000 to 2009. Microbial PLFAs were analysed from soils sampled in 2009. Soil microbial diversities showed PLFAs of monoplanting of pepper were distinct from those of monoplanting of garlic and interplanting of garlic and pepper by principle component 2 (PC2). Furthermore, soil microbial activity of monoplanting of pepper significantly decreased PLFAs representing as VAM-fungi, whereas it significantly increased in actinomycetes and saturated/monounsaturated PLFAs' ratio. The results drove continuous cropping of pepper would vary the microbial community and their specific activity. Soil microbial activities in continuous cropping system would depend on crop root systems.