Application of UV Technology for Surface Disinfection

표면 살균을 위한 UV 기술의 적용

  • Cho, Min (School of Civil and Environmental Engineering, Georgia Institute of Technology) ;
  • Jeong, Woo-Dong (School of Chemical and Biological Engineering, Seoul National University) ;
  • Yoon, Je-Yong (School of Chemical and Biological Engineering, Seoul National University)
  • 조민 (조지아공대 토목환경공학부) ;
  • 정우동 (서울대학교 화학생물공학부) ;
  • 윤제용 (서울대학교 화학생물공학부)
  • Published : 2007.09.30

Abstract

The control of pathogenic microorganisms is important issue in human environments, especially in surface area. However, surface disinfection has not been fully researched. In this study, the surface disinfection under UV irradiation was performed to investigate the quantitative kinetics for Bacillus subtilis spore inactivation in several experimental conditions, such as light intensity, temperature and surface roughness. This study reports that UV light would apparently inactivates the microorganisms and the required IT value for 2 log (99%) Bacillus subtilis spore inactivation was found to be 14.5 $mJ/cm^2$ in plain surface, as predicted by the Delayed Chick-Watson model. When UV was irradiated, there were no significant temperature effects. However, the experimental result shows that the more increased IT values are required at larger surface roughness.

전 세계적으로 병원성 미생물에 의한 오염과 감염 및 발병의 사례가 급증함에 따라 병원성 미생물을 효율적으로 제어하는 기술에 대한 관심이 매우 증가하고 있다. 병원성 미생물을 환경에서 제어하는 연구는 대다수 수처리 공정과 같이 물에서 이루어지는 소독과 관련되어 진행되어 왔고, 표면에서의 소독에 관련된 연구는 매우 부족하였다. 본 연구는 UV 소독 방법을 표면에 분포된 미생물의 불활성화에 대해서 적용하여 살펴보았고, 소독 모델을 통해서 정량화하였다. 특히, 생물테러용 무기로 잘알려진 탄저균(Bacillus anthracis)의 지표 미생물인 바실러스 포자(Bacillus subtilis spore)에 대해서 UV를 이용한 표면 소독시 매우 빠른 불활성화를 볼 수 있었으며, 2 log(99%) 불활성화를 위한 IT 값은 14.5 $mJ/cm^2$으로 나타났다. UV에 의한 미생물 불활성화는 화학적 소독제의 경우와 달리 온도에 의한 영향은 나타나지 않았으나, 표면 거칠기(surface roughness)에 의한 뚜렷한 tailing off 현상이 나타났다.

Keywords

References

  1. Meakins, S. M., Adak, G. K., Lopman, B. A., and O'Brien S. J., 'General outbreaks of infectious intestinal disease(IID) in hospitals, England and Wales, 1992-2000,' J. Hospital Infection, 53, 1-5(2003) https://doi.org/10.1053/jhin.2002.1326
  2. Makino, S. I., Kawamoto, K., Takeshi, K., Okada, Y., Yamasaki, M., Yamamoto, S., and Igimi, S., 'An outbreak ef food-borne listeriosis due to cheese in Japan, during 2001,' Int. J. Food Microbial., 104, 189-196 (2005) https://doi.org/10.1016/j.ijfoodmicro.2005.02.009
  3. Yildiz, F. H., 'Processes controlling the transmission of bacterial pathogens in the environment,' Research in microbiology, 158, 195-202(2007)
  4. Belosevic, M., Craik, S. A., Stafford, J. L., Neumann, N. F., Kruithof, J., and Smith, D. W., 'Studies on the resistance/reactivation of Giardia muris cysts and Cryptosporidium parvum oocysts exposed to medium-pressure ultraviolet radiation,' FEMS Microbial. Lett., 204, 197-203(2001) https://doi.org/10.1111/j.1574-6968.2001.tb10885.x
  5. Cho, M., Chung, H., and Yoon, J., 'Quantitative Evaluation of the synergistic Sequential inactivation of Bacillus subtilis spores with Ozone followed by Chlorine,' Environ. Sci. Technol., 37, 2134-2138(2003) https://doi.org/10.1021/es026135h
  6. Cho, M., Lee, Y., Choi, W., Chung H., and Yoon, J., 'Study on Fe(VI) species as a disinfectant: quantitative evaluation and modeling for inactivating Escherichia coli,' Water Res., 40, 3580-3586(2006) https://doi.org/10.1016/j.watres.2006.05.043
  7. Pedazhur, R., Katzenelson, D., Barnea, N., Lev, O., Shuval, H. I., Fattal, B., and Ulitzur, S., 'The efficacy of long-lasting residual drinking water disinfectants based on hydrogen peroxide and silver,' Water Sci. Technol., 42, 293-298(2000)
  8. Sternbach, G., The history of anthrax, J Emergency Medicine, 24, 463-467(2003) https://doi.org/10.1016/S0736-4679(03)00079-9
  9. Jamieson, D. J., Ellis, J. E., Jernigan, D. B., and Treadwell, T. A., 'Emerging infectious disease outbreaks: Old lessons and new challenges for obstetrician-gynecologists,' Am. J. Obstet. Gynecol., 197, 1546-1555(2006)
  10. Greene, A. K., Few, B. K., and Serafini, J. C., 'A comparison of ozonation and chlorination for the disinfection of stainless steel surfaces,' J Dairy Sci., 76, 3617-3620(1993) https://doi.org/10.3168/jds.S0022-0302(93)77702-4
  11. Ronner, A. B. and Wong, A. C. L., 'Biofilm development and sanitizer inactivation of Listeria monocytogenes and Salmonella typhimurium on stainless steel and buna-n rubber,' J. Food Protect., 56, 750-758(1993) https://doi.org/10.4315/0362-028X-56.9.750
  12. Andrews, S., Pardoel, D., Harun, A., and Treloar, T., 'Chlorine inactivation of fungal spores on cereal grains,' Int. J. Food Microbiol., 35, 153-162(1997) https://doi.org/10.1016/S0168-1605(96)01214-7
  13. Foschino, R., Nervegna, I., Motta, A., and Galli, A., 'Bactericidal activity of chlorine dioxide against Escherichia coli in water and on hard surfaces,' J. Food. Prot., 62, 668-672(1998)
  14. Cho, M., Kim, J., and Yoon, J., 'Investigating synergism during sequential inactivation of Bacillus subtilis spores with several disinfectants,' Water Res., 40, 2911-2920(2006) https://doi.org/10.1016/j.watres.2006.05.042
  15. 조민, 김지연, 윤제용, 'UV 조사(Ultraviolet Irradiation)에 의한 미생물의 불활성화,' 첨단환경기술, 2, 5-18 (2004)
  16. Masschelein, W. J., 'Ultraviolet light in water and wastewater sanitation,' LEWIS publishers(2002)
  17. Cockell, C. S., Rettberg, P., Horneck, G., Wynn-Williams, D. D., Scherer, K., and Gugg-Helminger, A., 'Influence of ice and snow covers on the UV exposure of terrestrial microbial couumnities: dosimetric studies,' J. photochem. Photobiol. B: Biol., 68, 23-32(2002) https://doi.org/10.1016/S1011-1344(02)00327-5
  18. Rochelle, P. A., Upton, S. J., Montelone, B. A., and Woods, K., 'The response of Cryptosporidium parvum to UV light,' TRENDS in Parasitology, 21, 81-87 (2005) https://doi.org/10.1016/j.pt.2004.11.009
  19. USEPA, 'Alternative disinfectants and oxidants guidance manual,'(1999)
  20. USEPA, 'Ultraviolet disinfection guidance manual,'(2003)
  21. Cho, M., Chung, H., Choi, W., and Yoon, J., 'Different inactivation behaviors of MS-2 phage and Escherichia coli in $TiO_22$ photocatalytic disinfection,' Appl. Environ. Microbiol., 71, 270-275(2005) https://doi.org/10.1128/AEM.71.1.270-275.2005
  22. Sonenshein, A. L., Hoch, J. A., and Losick, R., 'Bacillus subtilis and other gram positive bacteria,' ASM, Washington, D. C.(1993)