• The Korean Journal of Ecology
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• v.22 no.3
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• pp.109-117
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• 1999

Thirty two species of coastal plants (mainly halophytes) were investigated for their free amino acids and the total and water-soluble nitrogen contents in leaves. All plants except some species (e.g. Scutellaria and Linaria) contained proline, but only Ageratum. Tetragonia and Raphanus in rather high amounts, that it can be thought to serve as a cytoplasmic osmolyte. In some plant species (Euphorbia. Glehnia. Peucedanum. Raphanus and monocotyledonous Carex and Zoysia), however, hydroxyproline. (OH-Pro) rather than proline were accumulated to a considerable extent. The concentrations of total free amino acids were low in Aster tripolium, Linaria. Lysimachia. Plantago. Rumex, Vitex and especially in the members of the Chenopodiaceae and Crassulaceae. Marked differences also occurred in the nitrogen levels. Aizoaceae, Chenopodiaceae, Convolvulaceae, Cruciferae, Euphorbiaceae and Leguminosae usually showed high values of total and soluble amino nitrogen, while the opposite was true for most of the Cyperaceae, Gramineae, Lamiaceae, Plantaginaceae, Scophuriaceae, and Verbenaceae. The free amino acids in the investigated plant species contributed very little to the nitrogen content, but in plants of Euphorbia, Messerschmidia and Orostachys. their amino acid-N made up for 25∼30% of the total nitrogen. In conclusion, only a few cases did proline known as compatible solute constitute a significant proportion of the free amino acid pool in coastal plants.

• Korean Journal of Medicinal Crop Science
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• v.22 no.6
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• pp.435-441
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• 2014

This study was conducted to investigate the quality of seeds, the germination rates and the days required for germination, to examine the patterns of protein expressions during the germination and to improve the techniques of managing and storing seeds and viability of the seeds of Lithospermum erythrorhizon Sieb. et Zucc. After collecting and harvesting seeds, they were classified to white and brown colors of seed coat through testing their seed size, weight, and quality. The germination rates, the days required for germination, and the protein expressions were examined with different colors of seed coats, storing temperatures and durations by treating the different plant growth regulators and primings. One hundred seed weight of white color was heavier about 1.17 g than those of brown one about 0.81 g. The germination rates in white color of seed coat was higher, 3.05 ~ 5.75%, than those in brown one. Its rates were decreased with getting longer in storage durations. There was no big differences on germination rates between storage temperatures. The plant growth regulator of $GA_3$ and Kinetin was affected to improve the seed germination. $GA_3$ increased the seed germination clearly at 25 ppm level, while kinetin increased it gradually from 25 to 100 ppm levels. In germination by seed primings, PEG6000 made higher germination rate with increasing their levels, whereas $KNO_3$ increased the germination until 100 mM level and then decreased it with 200 mM unlike PEG6000. The protein expressed during the seed germination were appeared more and clearer bands in the seed after germination, especially 20 ~ 30 kDa, compared to those in the seed before germination. These results showing more and clearer bands were positively related to the germination rates which were different by seed colors, storage temperatures and durations, and plant growth regulators and primings.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.32 no.4
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• pp.50-60
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• 2014

This study aims to sort out the plants in "Poetry of the South" what is in the written by Qu Yuan in the Kingdom of Chu and to analyze the utility and appreciation of plants. The conclusions are as follows. First, there were 80 plant names and 56 plant species were sorted out, and these results can confirm that the plants were existed in the 340-277 BC. Second, through analyzing the utility of the plants, herba lycopi, basil etc. as the garden plants; lotus, chrysanthemum etc. as the ornamental plants; galangal and cannabis sativa as the present; basil, musa basjoo, etc. as the sacrifice plants. Third, through analyzing the appreciation of the plants, golden birch, chrysanthemum etc. for gustatory appreciation; herba lycopi and pine tree etc. for tactile appreciation; bamboo for sensory appreciation. Forth, through analyzing the token of the plants, calamus and basil etc. are the symbol of personal character noble; wormwood and caltrop etc. are the symbol of villain. Based on the research of "Poetry of the South", it can help to understand the plants culture in 340-277 BC, and it will be a useful information for the plant design.

• Journal of the Korean Wood Science and Technology
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• v.35 no.3
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• pp.70-78
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• 2007

This study explored the efficient dyeing method of aged chestnut wood using the natural dyes extracted from Gardenia jasminoides for, grandiflora, Carthamus tinctorius L., Rhus javanica L., Lithospermum erythrorhizon S. et Z., Caesalpinia sappan L. and Castanea crenata S. et Z.. The color variation of the dyed chestnut woods was also quantitatively evaluated. The wood specimens revealed abundant colors through different dyeing conditions such as dyeing temperature, dye pH, soaking time, and brushing frequency. The chroma and stain concentration of colored woods were calculated with $L^*$, $a^*$ and $b^*$ in order to make color tone measurement. Brushing treatment for colored wood was confirmed as a more economical dyeing method than soaking treatment requiring more time when natural dyeing was done. Furthermore, the desirable tone and shade of color was easily obtained by repetitive brushing treatment using low color tone. During natural dyeing, good color expression was made with high temperature and acidic condition of a dyeing liquor.

• Korean Journal of Plant Resources
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• v.25 no.1
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• pp.80-88
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• 2012

This study was conducted to clarify the antimicrobial effects and radical scavenging activities of the cosmetic compositions having the natural plant pigments, and to enhance the natural materials utilization of cosmetics. The antimicrobial activities of the fifteen kinds of cosmetic composition having natural plant pigments were evaluated using the agar diffusion method. Most of the cosmetic composition having the natural pigments showed the clear zone formation of growth inhibition against Bacillus subtilis, Staphylococcus aureus, Listeria monocytogenes, Vibrio parahaemolyticus and Aspergillus flavus. Especially, purple sweet potato, bitter melon, mulberry leaf and gromwell showed the higher antimicrobial activities. All the cosmetic compositions were evaluated for their antioxidant activity using DPPH radical scavenging capacity and nitrite scavenging ability activity. Both of the free radical DPPH and nitrite scavenging ability was the highest in the cosmetic compositions of onion peel, and these antioxidant activity was significant differences according to different plant pigments. In this study, we conjectured that the plant pigment had the potent biological activities, therefore these plant resources having functional components could be a good materials for development into source of natural cosmetics.

• Korean Journal of Medicinal Crop Science
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• v.27 no.4
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• pp.247-258
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• 2019

Background: Astragali radix (A) and Lithospermi radix (L) have long been used as traditional medicines due to their known anti-inflammatory effects. This study aimed at evaluating, their optimal mixing ratio and their functional compounds by investigating the inhibitory effects of mixed extracts of A and L and their active compounds on matrix metalloproteinases (MMPs). Methods and Results: A and L extracts were obtained by extraction at $80^{\circ}C$ using 50% and 70% fermented alcohol, respectively, and then mixed at a ratio of 5 : 5, 6 : 4, 7 : 3 and 8 : 2 (w/w). The activities of MMP-1, MMP-3, and MMP-13 were evaluated in interleukin-1beta ($IL-1{\beta}$)-induced SW1353 cells. The extract mixtures showed synergistic inhibitory effects on MMP-3 and MMP-13, higher than the effects of the individual A and L extracts. The 7 : 3 mixture (ALM16) showed the most effective MMPs inhibitory activity, while among the active ingredients, calycosin-7-O-${\beta}$-D-glucoside and lithospermic acid exhibited excellent MMPs inhibitory activity. Additionally, an HPLC method was established for simultaneous quantification of the effective components of the extract mixtures, and validated by measuring the linearity, precision and accuracy of the limit of detection (LOD) and limit of quantification (LOQ). Conclusions: ALM16 showed the most effective MMPs inhibitory activity. Calycosin-O-${\beta}$-D-glucoside, calycosin and lithospermic acid were identified as useful candidates, as they were the major functional compounds in the MMP inhibitory activity. Summarily, ALM16 might be a highly effective in osteoarthritis management, owing to its because it exhibits a protective effect on cartilage via excellent inhibition of MMPs.

• Korean Journal of Environment and Ecology
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• v.36 no.1
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• pp.30-55
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• 2022

This study conducted a flora survey in the areas of Mt. Sambong (282m) and Mt. Jaung (263m), located in Paju-si, Gyeonggi-do. A total of 434 taxa, including 6 forms, 34 varieties, 11 subspecies, 383 species, 271 genera, and 94 families, were surveyed in the two mountain areas. The ferns were identified as 24 taxa from 10 families, the gymnosperms as 8 taxa from 2 families, the dicotyledonous plants as 322 taxa from 73 families, and the monocotyledonous plants as 80 taxa from 9 families. Five taxa, including Salix koriyanagi, Cimicifuga heracleifolia, Forsythia koreana, Paulownia coreana, and Hemerocallis hakuunensi, were identified as the Korean endemic plants. Eight taxa, including Cimicifuga heracleifolia, Tylophora floribunda, and Iris minutiaurea as Vulnerable species, andAristolochia contorta, Viola diamantiaca, Lithospermum erythrorhizon, Lilium distichum, andArisaema heterophyllumas Least Concern species, were designated as rare and endangered plants designated by the Korea Forest Service. The invasive alien plants were 40 taxa, including Rumex crispus, Phytolacca americana, Cerastium glomeratum, Silene armeria, Chenopodium album, Chenopodium ficifolium, Magnolia obovata, Potentilla supina, Amorpha fruticosa, Medicago sativa, Robinia pseudoacacia, Trifolium repens, Euphorbia supina, Hibiscus trionum, and Oenothera biennisaccounting for 9.2 percent of the 434 taxa of the surveyed plants. As this area is a mountainous area close to settlement facilities, it is believed that ecological preservation measures and measures of specialized plants should be implemented.

• (The)Study of the Eastern Classic
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• no.34
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• pp.39-62
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• 2009

In this paper, the researcher investigated the standpoint of Lee EonJeok(李彦迪), also known as HoeJae(晦齋), with regard to interpretation of Daxue("大學"), through Daxuezhangjubuyi("大學章句補遺") and XuDaxuehuowen("續大學或問"). It is true that HoeJae accommodates a fairly large portion of interpretations made by Zhuzi, however, he strived to pass over Zhuzi's Daxuezhangju by attempting a new interpretation on the Gewuzhizhi(格物致知). The greatest reason that HoeJae couldn't admit Zhuzi's view was derived from the differences in interpretation of Gewuzhizhi. The differences between Zhuzi and HoeJae concerned with Gewuzhizhi is that, while Zhuzi perceived the objects thereof from the perspective of Li(理) of things(事物), HoeJae considered the root and branch, the beginning and end of the physical world(萬物) and also all events(萬事) as the subjects of Gewuzhizhi. Meanwhile, having the chapter, dubbed, 'Weyoubenmo'(物有本末), and the chapter, 'ZhiZhi'(知止) also as expositions of Gewuzhizhi, HoeJae was able to avoid such critiques as that he complemented or added new topics to the sutra. In addition, he could have clarified the stepwise elucidations of Sangangling(三綱領) and Batiaomu(八條目) edited and compiled by Zhuzi(朱子). And the issues in the chapter of Tingsong(聽訟) lies on the extended line of theory of Gewuzhizhi. In the end, it suggests that the differences between Zhuzi and HoeJae are arisen from the discrepancies of interpretation on Gewuzhizhi. HoeJae proposed his ideology concerned with Zhizhizhuyi(至治主義) through his book, XuDaxuehuowen, and he stressed that Ren(仁) must be foundation in order to Pingtianxia(平天下). Furthermore, HoeJae emphasized that Ren which is the founding virtue in order for Pingtianxia must be begun with such very basic behavioral practices, known as, Xiao(孝), Ti(弟), and Ci(慈), and that such desirable states would be accomplished through relationships with others, not merely through self-endeavors or by self-ego.

• Korean Journal of Environment and Ecology
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• v.23 no.2
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• pp.177-186
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• 2009

In the flora of the whole area of Jakeundang at Byeonsanbando National Park, a total of 105 taxa were identified including 50 families, 88 genera, 98 species, 6 varieties and 1 form. These were again divided into 31 taxa (29.5%) of woody plants and 74 taxa (70.5%) of herbaceous plants. In addition, 4 taxa of the Korea Forest Service, designated rare plants in the flora were identified to grow there including Phacelurus latifolius (Preservation priority order: No. 194), Aristolochia contorta (No. 151), Koelreuteria paniculata (No. 115), and Glehnia littoralis (No. 203). Indigofera koreana, Viola seoulensis that are on the list of Korean endemic plants were also identified and the plants approved for export were identified similarly to rare plants. In addition, among the floral region-based specific plants, the plant group belonging to class V, class IV and II didn't appear and a total of 16 taxa 3 taxa including Elymus mollis, Salsola collina and Koelreuteria paniculata in class III, and 13 taxa including Ischaemum anthephephoroides, Phacelurus latifolius, Asparagus cochinchinensis, Melandryum oldhamianum for. roseum, Calystegia soldanella, Messerschmidia sibirica, Scutellaria strigillosa and Aster spathulifolius, etc. in class I were identified. Among the naturalized plants in the surveyed sites, 6 families, 10 genera, and 10 taxa were identified to grow, such as Bromus unioloides, Phytolacca americana, Lepidium apetalum, Robinia pseudoacacia, Oenothera biennis and Xanthium canadense, etc. and the naturalization rate was analyzed to be 9.5% of all 105 taxa of vascular plants. This research analyzed the coverage ratio and importance value by dividing the community into a sand dunes and back swamp and divided the sand dune area into Vitex rotundifolia community and the back swamp into Phacelurus latifolius community. Jakeundang is designated as a sand dune plants observation area and managed by the office of Byeonsanbando National Park. To preserve this place, it is desirable to take a systematic management measure, such as a visit to this place on a subscription basis or education and publicity on sand dune plants.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.36 no.3
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• pp.186-196
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• 2010

Introduction: The first aim of this study was to isolate the dental tissue-derived stem cells from the dental follicle (DF), dental pulp (DP), and root apical papilla (RAP) of the extracted wisdom teeth. Second was to evaluate their characterization with the expressions of transcription factors and cell surface markers. Finally, their ability of the in vitro multi-lineage differentiations into osteogenic and adipogenic cells were compared, respectively. Materials and Methods: Dental tissues, including dental follicle, dental pulp, and root apical papilla, were separated in the extracted wisdom teeth. These three dental tissues were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with supplements, respectively. After passage 3, the homogeneous shaped dental tissue-derived cells were analyzed the expression of transcription factors (Oct-4, Nanog and Sox-2) and cell surface markers (CD44, CD90 and CD105) with reverse transcription polymerase chain reaction (RT-PCR) and fluorescence-activated cell sorting (FACS) analysis. In order to evaluate in vitro multi-lineage differentiations, the culture media were changed to the osteogenic and adipogenic induction mediums when the dental tissue-derived cells reached to passage 3. The characteristics of these three dental tissue-derived cells were compared with immunohistochemistry. Results: During primary culture, heterogenous and colony formatted dental tissue-derived cells were observed in the culture plates. After passage 2 or 3, homogenous spindle-like cells were observed in all culture plates. Transcription factors and mesenchymal stem cell markers were positively observed in all three types of dental tissue-derived cells. However, the quantity of expressed transcription factors was most large in RAP-derived cells. In all three types of dental tissue-derived cells, osteogenic and adipogenic differentiations were observed after treatment of specific induction media. In vitro adipogenic differentiation was similar among these three types of cells. In vitro osteogenic differentiation was most strongly and frequently observed in the RAP-derived cells, whereas rarely osteogenic differentiation was observed in the DP-derived cells. Conclusion: These findings suggest that three types of human dental tissue-derived cells from extracted wisdom teeth were multipotent mesenchymal stem cells, have the properties of multi-lineage differentiations. Especially, stem cells from root apical papilla (SCAP) have much advantage in osteogenic differentiation, whereas dental follicle cells (DFCs) have a characteristic of easy adipogenic differentiation.