• Proceedings of the KSEEG Conference
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• 2007.04a
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• pp.649-651
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• 2007

• Journal of Life Science
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• v.13 no.4
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• pp.463-472
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• 2003

Interactions between factor Va (HFVa) and membrane phosphatidylserine (PS) regulate the activity of the prothrombinase complex. I have previously shown that two solvent exposed hydrophobic residues located in the C2-domain, Trp2063 and Trp2064, are required for binding to immobilized PS and for expression of procoagulant activity on membranes containing 5% PS. In order to fully define the functional importance of these two residues I have expressed and isolated recombinant factor Va (rHFVa) W2063A/W2064A double mutant. In contrast to the native protein the two glycoforms resulting from alternative glycosylation of Asn2181 eluted as a single peak with rHFVa1 W2063A/W2064A eluting on the leading edge and rHFVa2 W2063A/W2064A eluting on the trailing edge. The double mutant rHFVa2 W2063A/W2064A expressed little or no procoagulant activity on membranes containing 1-10% mol % PS. In contrast, the procoagulant activity of this mutant was slightly greater than the native protein on membranes containing>18 mol % PS. The binding of rHFVa2 W2063A/W2064A to immobilized phospholipid vesicles was markedly reduced compared to the native protein in a surface plasmon resonance binding assay. I conclude that Trp2063 and Trp2064 are required for high affinity binding of factor Va to PS membranes and that this interaction is necessary for assembly of the prothrombinase complex on membranes containing physiological concentrations of PS.

• The Journal of the Convergence on Culture Technology
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• v.8 no.3
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• pp.551-557
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• 2022

Geotechnical risk factors encountered in intermediate-depth underground tunnel construction are diverse, and the types and standards of risk factors are different according to the depth and regional geological characteristics of Korea. In order to understand the effects of geological characteristics and geologic structure on safety, which show various porous characteristics of urban underground complex ground, the risk factors of intermediate-depth rock mass in Korea were analyzed based on domestic and foreign cases. As a result of the study, seven categories affecting the stability of the intermediate-depth tunneling, namely, geologic structure, rock characteristics, hydrogeology, overburden, high stress, ground characteristics and artificial structures, and about 22 risk factors were derived. We present the risk criteria and interval values for risk evaluation of faults, folds, dikes, and rocks that have the greatest influence among risk factors. Criteria and interval values for other risk factors are under study.

• The Journal of Engineering Geology
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• v.27 no.2
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• pp.153-164
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• 2017

The geological factors for causing ground subsidence are very diverse. It can be affected by any geological or extrinsic influences, and even within the same geological factor, the soil depression impact factor can be determined by different physical properties. As a result of reviewing a large number of papers and case histories, it can be seen that there are seven categories of ground subsidence factors. The depth and thickness of the overburden can affect the subsidence depending on the existence of the cavity, whereas the depth and orientation of the boundary between soil and rock are dominant factors in the ground composed of soil and rock. In case of soil layers, more various influencing factors exist such as type of soil, shear strength, relative density and degree of compaction, dry unit weight, water content, and liquid limit. The type of rock, distance from the main fracture and RQD can be influential factors in the bedrock. When approaching from the hydrogeological point of view, the rainfall intensity, the distance and the depth from the main channel, the coefficient of permeability and fluctuation of ground water level can influence to ground subsidence. It is also possible that the ground subsidence can be affected by external factors such as the depth of excavation and distance from the earth retaining wall, groundwater treatment methods at excavation work, and existence of artificial facilities such as sewer pipes. It is estimated that to evaluate the ground subsidence factor during the construction of underground structures in urban areas will be essential. It is expected that ground subsidence factors examined in this study will contribute for the reliable evaluation of the ground subsidence risk.

• The Journal of Engineering Geology
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• v.33 no.4
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• pp.611-626
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• 2023

Overseas examples of the characterization stage of site selection proposed by the International Atomic Energy Agency were reviewed to highlight the factors necessary for consideration in the deep disposal of high-level radioactive waste. Studies in Sweden, Finland, the USA, and Canada were considered. Site investigations in Sweden and Finland commonly covered the fields of geology, hydrogeology, and hydrogeochemistry using similar field investigation techniques. The USA considered survey groups and factors under pre- and post-lockdown guidelines, as well as those for desaturated and saturated surveys. involving geophysical, hydrological, hydrogeological, hydrogeochemical, mechanical/physical, and thermal-characterization investigations. Canada provided a list of investigative methods for both preliminary and detailed site assessments including geological, physical, boring, hydrological, laboratory testing, and chemical analysis studies. Results of this study should elucidate site-selection investigation factors and survey methods applicable to Korea.

• Tunnel and Underground Space
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• v.32 no.6
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• pp.530-548
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• 2022

The site selection process for deep geological disposal of high-level radioactive waste will be conducted in stages, and 103 evaluation parameters related to site selection have been proposed. In the field of rock mechanics and rock engineering, there are 33 evaluation parameters for intact rock, joint and rock mass, and they are applied in the basic and detailed investigation stages. In this report, uniaxial compressive strength, in-situ stress, joint distribution, and rock mass classification were selected as the main evaluation parameters, and among them, uniaxial compressive strength and in situ stress were selected as key evaluation parameters. Statistical techniques or regression analysis were performed for granite in Wonju and Chuncheon to evaluate the distribution range for the selected key evaluation parameters. The average of the uniaxial compressive strength in the Wonju area estimated through the posterior distribution is about 171 MPa, and about 123 MPa in the Chuncheon area. The maximum in situ stress acting in the Wonju area was less than 30 MPa and less than 40 MPa in the Chuncheon area. The direction of the maximum horizontal stress calculated by regression analysis was 101° in Wonju, and in the case of Chuncheon, it was 95°, respectiviely.

• The Journal of Engineering Geology
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• v.16 no.1 s.47
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• pp.85-96
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• 2006

This study was conducted to suggest a method to determine weighting values of each parameter of the RMR system considered with geologic characteristics of a study area. This study reviewed the weighting values of the RMR system for the Cretaceous sedimentary rocks distributed in Ulsan area. Based on the data of field survey at the study area, a multiple regression analysis was used to set up an optimal weighting values of the RMR parameters. For the multiple regression analysis, each parameter of the RMR and the slope gradient were regarded as the independent variable and the dependent variable, respectively. The analysis result suggested a modified weighting values of the RMR parameters as follows; 30 for the intact strength of rock; 18 for RQD; 8 for spacing of discontinuities; 32 for the condition of discontinuities; and 12 for ground water.

• Proceedings of the KSEG Conference
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• 2000.04a
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• pp.165-173
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• 2000

• Proceedings of the KSEEG Conference
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• 2000.04b
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• pp.182-183
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• 2000

• Applied Chemistry for Engineering
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• v.28 no.3
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• pp.306-312
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• 2017

Changes in the cell growth and lipid accumulation of marine microalga Dunaliella tertiolecta were investigated in response to the combination of different stress factors including the variation of iron supply as a primary stress factor and different options in light irradiation and $CO_2$ supply as a secondary stress factor. High or limited Fe conditions could act as a stress for lipid synthesis. As a secondary stress factor, non-$CO_2$ condition was good for lipid accumulation, but the overall cell growth was sacrificed significantly after a long-time cultivation. Dark condition as a secondary stress factor also favored lipid accumulation and the extent of cell density reduction at the early period in the dark was small compared to other stress conditions. The two-stage cultivation strategy was necessary to maximize lipid production because tendencies of the cell growth and lipid content were not identical under the chosen stress condition. The first stage was for preparing a high cell density under the normal growth-favoring condition and the second stage was the stress condition to induce lipid accumulation in a short time. The short-term (12 h) incubation under the 5X Fe (3.25 mg/L) and dark conditions resulted in the best lipid productivity of 1.44 g/L/d providing 2 g/L inoculum at the second stage.