• Journal of Life Science
• /
• v.28 no.3
• /
• pp.307-313
• /
• 2018

Alopecia cause psychological stress due to their effect on appearance. Thus, the global market size of the alopecia treatment products are growing quickly. Timosaponin A III is the well known active ingredient of Anemarrhenae Rhizoma. In this study, we investigated and compared the binding affinity of timosaponin A III with finasteride (5-beta reductase antagonist) and minoxidil (androgen receptor antagonist) on the target protein active site by in silico computational docking studies. The three dimensional crystallographic structure of 5-beta reductase (PDB ID : 3G1R) and androgen receptor (PDB ID: 4K7A) was obtained from PDB database. In silico computational autodocking analysis was performed using PyRx, Autodock Vina, Discovery Studio Version 4.5, and NX-QuickPharm option based on scoring functions. The timosaponin A III showed optimum binding affinity (docking energy) with 5-beta reductase as -12.20 kcal/mol as compared to the finasteride (-11.70 kcal/mol) and with androgen receptor as -9.00 kcal/mol as compared to the minoxidil (-7.40 kcal/mol). The centroid X, Y, Z grid position of the timosaponin A III on the 5-beta reductase was similar (overlap) to the finasteride, but the X, Y, Z centroid grid of the timosaponin A III on the androgen receptor was significantly far from the minoxidil centroid position. These results significantly indicated that timosaponin A III could be more potent antagonist to the 5-beta reductase and androgen receptor. Therefore, the extract of Anemarrhenae Rhizoma or timosaponin A III containing biomaterials can substitute the finasteride and minoxidil and can be applied to the alopecia protecting product and related industrial fields.

• Herbal Formula Science
• /
• v.16 no.1
• /
• pp.39-53
• /
• 2008

This report describes 68 studies related to the use of Rhizoma Anemarrhenae main blended prescription from Dongeuibogam. The following conclusion were reached through investigations on the prescriptions that use Rhizoma Anemarrhenae as a key ingredient. Prescriptions that Rhizoma Anemarrhenae was taken as a monarch drug are utilized for 21 therapeutic purposes, for example, cough, malaria, Sang Han syndromes and eye disease. In particular, 22.1% of prescriptions appear in the chapter of cough, and 13.2% of those appear in the chapter of malaria. Prescriptions that utilize Rhizoma Anemarrhenae as the main ingredient are used in the treatment of cough, asthma and malaria. Rhizoma Anemarrhenae is used in pathogenic factors such as fire, and used in pathology related to kidny system. The dosage of Rhizoma Anemarrhenae is 3bun(about 1.12 gram) to 3jeon(about 11.25 gram), however 1jeon(about 3.75 gram) has been taken the most for clinical application. Gamlihuan is the most useful base prescription which use the Rhizoma Anemarrhenae as the main ingredient.

• Journal of Pharmaceutical Investigation
• /
• v.15 no.2
• /
• pp.73-82
• /
• 1985

Each parching Anemarrhenae Rhizoma with 25% ethanol and normal saline has been used for anti-inflammatory, expectorant, antipyretic, sedative and diuretic, and so on. In order to investigate the differences of referential efficacy about each fractionated part of the aqueous extracts, pharmacological studies were carried out. The results of studies were summerized as follows : 1. By the administration of 25% ethanol treated preparation (F-I-1), the increase in urinary volume and $Na^+$ extraction was significantly recognized. 2. The excretion of electrolyte $Na^+$ by saline solution treated preparation (F-II-1) was significantly recognised in normal rats. 3. The increase of urinary volume and urinary $Na^+$ and $Cl^-$ excretion by F-I-1 and n-butanol insoluble parts of F-I-1 (F-I-3) was significantly recognized in $HgCl_2-induced$ acute renal failure of rats. 4. The increase of plasma $Na^+$ by F-I-1 and urea nitrogen by F-I-1 and F-I-3 in acute renal failure of rats was significantly recognized. 5. The increase of urinary volume by F-I-1 and F-I-3 was recognised in mice.

• Korean Journal of Pharmacognosy
• /
• v.26 no.1
• /
• pp.66-73
• /
• 1995

'Gyejakjimo-Tang(桂芍知母湯)', which is composed of Cinnamon bark(桂枝), Peony root(芍藥), Anemarrhena root(知母) and other seven herbs, is described as antipyretic, diuretic and analgesic prescription in traditional medical literatures including Geumgwe-Yoryak(金櫃要略). So it is being used in the treatment of gout clinically in oriental region. As the results of the pharmacological and biochemical trials of the prescription, it was found to have antiinflammatory and significant analgesic effects indicated by carrageenin edema, dye permeabilities and writhing behavior. And it potentiated the output of uric acid in blood and the increase of uric acid in urine of alcohol-toxicated rats, which mean the decrease of uric acid level only by excretion procedure. But the prescription didn't show any effects on the activities of adenine/guanine deaminase, purine nucleoside phosphorylase, xanthine oxidase or uricase, which are related with formation and metabolism of uric acid.

• The Journal of Internal Korean Medicine
• /
• v.28 no.3
• /
• pp.608-614
• /
• 2007

Objectives : This study was carried out to investigate anti-proliferative effects on MCF-7 human breast cancer cells of Anemarrhenae Rhizoma (AR) extract. Breast cancer is the most common disease in Korean women. Despite remarkable improvements in treatment strategies against various cancers during the past 40 years. breast cancer still remains as one of the main causes of cancer mortality among women the whole world over. Methods : Be investigated the effects of AR on cytotoxicity of MCF-7 human breast cancer cells in various extract conditions (n-hexane, ethyl acetate, butanol and water fraction). Results : The extract of Anemarrhenae Rhjzoma inhibits the proliferation of MCF-7 cells in a dose dependent manner. Especially. the ethyl acetate fraction of Anemarrhenae Rhizoma showed specific Cytotoxicity on MCF-7 cells. Conclusions : In conclusion. it can be concluded that Anemarrhenae Rhizoma extract has an anti-proliferative effect on MCF-7 human breast cancer cells. Especially. the ethyl acetate fraction is most effective to inhibit proliferation of MCF-7 cells.

• Journal of Pharmaceutical Investigation
• /
• v.16 no.1
• /
• pp.18-23
• /
• 1986

Anemarrhenae Rhizoma has been clinically used as a sedative drug in oriental medicine. We observed the sedative action and effect on the movement of isolated-ileum with each water extract of Anemarrhenae Rhizoma (F-I), saline solution treated preparation (F-II) and 25% ethanol treated preparation (F-III). The results obtained were summerized as follows; 1) Sedative actions were recognized with F-I, F-II and F-III. 2) Prolonged effect of sleeping time was recognized significantly with F-I, F-II and F-III. 3) Each fraction of Anemarrhenae Rhizoma showed the released action on the isolated-ileum of the mouse, rat, rabbit and guinea pig, and antagonic action against contraction induced by acetylcholine, barium chloride and histamine. Thus the mechanism in the released action of the smooth muscle was partly considered as the direct dependence of the smooth muscle.

• 한국공간정보시스템학회:학술대회논문집
• /
• 2005.05a
• /
• pp.355-361
• /
• 2005

3차원 자료의 필요에 발맞추어 3차원 좌표를 직접적으로 획득할 수 있는 LIDAR 시스템이 등장하게 되었다 항공 LIDAR 시스템은 항공기, GPS, INS, Laser Scanner가 통합된 시스템으로 항공기에서 발사된 Laser의 반사파를 이용하여 거리와 그 때의 항공기의 자세, 위치를 통합하여 직접적인 3차원 포인트 자료를 획득할 수 있다. LiDAR 데이터는 지형, 건물, 식생 등의 지면위에 있는 모든 객체에 대한 3차원 자료와 영상자료를 함께 제공하고 있다. 이러한 LIDAR 자료로부터 DEM, DTM 등의 지형 정보와 식목, 건물 등 지물정보를 추출하는 연구가 활발하게 이루어지고 있다. 본 연구에서는 지형을 추출하는데 사용할 수 있는 몇 가지 필터링기법을 선정하여 국내의 다양한 지모, 지물에 적용하고 그 정확도를 평가해 보았다.

• 한국생물공학회:학술대회논문집
• /
• 2000.04a
• /
• pp.247-250
• /
• 2000

Extracellular polysaccharide was produced by Pseudomonas elodea ATCC 31461 under aerobic condition. Nitrogen sources in medium affected cell growth and production of exopolymer. Ammonium nitrate limitation was found to be essential for higher production of exopolymer. Conversion rate of exopolymer from glucose under ammonium nitrate limitation was about 5 times higher than with ammonium nitrate.

• Transactions of the Korean Society for Noise and Vibration Engineering
• /
• v.19 no.5
• /
• pp.475-481
• /
• 2009

Real-coded genetic algorithm(RCGA) has better performances than conventional genetic algorithm about dealing with a large domain, the precision and the constrain problem. Also the RCGA has advantage of operation time because it doesn't have to following about decoding operation. In this paper the ranges of PID gains are limited based on Ziegler-Nichols method to consider a long operation time problem that is the main problem of genetic algorithm. Result shows proposed method represents better performance without ignored about result of ZN tuning method and reduces the calculation time.

• Korean Journal of Pharmacognosy
• /
• v.30 no.2
• /
• pp.163-167
• /
• 1999

A method for isolation and qantitative determination of anemarsaponin B from the rhizomes of Anemarrhena asphodeloides has been developed. Isolation of anemarsaponin B was achieved by silica gel and RP-18 column chromatography. The HPLC method for quantitative determination of anemarsaponin B provided a method for standardization of the crude drug. It suggested that the content of anemarsaponin B in Anemarrhena asphodeloides is about 0.12-1.48%.