• Title/Summary/Keyword: 증식특성

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Isolation and Characterization of the Marine Bacterium, Alteromonas sp. SR-14 Inhibiting the Growth of Diatom, Chaetoceros Species (규조류 Chaetoceros sp. 증식 저해균 Alteromonas sp. SR-14의 분리 및 특성)

  • KIM Ji Hoe;PARK Jeong Heum;SONG Young Hwan;CHANG Dong Suck
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.32 no.2
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    • pp.155-159
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    • 1999
  • Marine bacteria inhibiting the growth of the diatom, Chaetoceros calcitrans were screened from seawater samples collected at south coast of Korea in 1996. Six strains were isolated from those samples. Among them, a bacterium SR-14 strain had the strongest inhibition activity against the alga. The selected SR-14 strain was identified as an Alteromonas sp. (supposed to be Alteromonas colwelliana) according to its biochemical results. Alteromonas sp. SR-14 was able to grow in raw seawater, aged seawater, Conwy medium for culture of microalgae and C. calcitrans culture filtrate. The host ranges of Alteromonas sp. SR-14 were C. calcitrans, C. muclleri and C. negracile among 10 species of diatom. All of the Chaetoceros spp. tested were inhibited by the Alteromonas sp. SR-14, However, the growth of the other genera in Bacillariophyceae was not inhibited.

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Studies on Growth Characteristics of lactobacillus brevis Isolated from Kimchi - Optimization of Nutrient Composition in Sourdough Media - (김치에서 분리한 Lactobacillus brevis의 생장 특성에 관한 연구( I ) - Sourdough 배지의 영양 조성 최적화 -)

  • 신언환
    • The Korean Journal of Food And Nutrition
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    • v.15 no.3
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    • pp.215-219
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    • 2002
  • Growth characteristics of sourdough lactic acid bacteria was investigated to obtain basic informations for sourdough starter. The optimum temperature and pH on bacterial growth and lactic acid production of Lactobacillus brevis UC-22 in sourdough broth were 35'E and around pH 5.5, respectively. And the optimum concentrations of the carbohydrate sources added to the broths was 2% maltose. The acidity significantly increased during growth by Lactobacillus brevis UC-22 fur 18 hours while pH significantly decreased during growth.

Establishment and Using of mass propagation system for Calanthe discolor Lindle (새우난초(Calanthe discolor Lindley)의 대량증식 시스템 확립 및 이용)

  • 서병기
    • Korean Journal of Plant Resources
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    • v.15 no.3
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    • pp.221-226
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    • 2002
  • This experiment was conducted to establish the mass propagation system of Calanthe discolor Lindley. When the Calanthe discolor seeds were sown in Murashige and Skoog medium, the percentage of germination was 65%. Seedlings grew more rapidly in the liquid medium than the solid medium. All regenerated plantlets were survived in acclimatized condition of 70% shade and more than 80% humidity. Also, we found out that the 88% of survival ratio could be achieved in containing soil mixture of vermiculite and perlite as same as amount.

Propagation of a New Anticancer Plant, Cephalotaxus koreana, by Cutting (신규 항암소재의 개비자나무의 삽목증식)

  • Jung, Myung-Suk;Hyun, Jung-Oh;Lee, Uk;Fadly Yahya, Andi;Baik, Eul-Sun
    • Journal of Korean Society of Forest Science
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    • v.98 no.4
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    • pp.491-495
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    • 2009
  • This study was conducted to investigate the propagation characteristics of Cephalotaxus koreana by cutting, whose plant has been known to new anticancer treatments. The treatments of plant growth regulators, cutting media temperature and growing media were investigated to know the rooting ability of cuttings. For rooting formation affected by plant growth regulators and its concentration, The application of IBA 100 mg/L was most effective in all of the characteristics including rooting rate of 88.5%. The optimum media temperature by cutting was $24^{\circ}C$ (treatment IBA 100 mg/L, rooting rate 84.6%), predicting that greenwood cutting from June to July is most effective. VPPL (vermiculite+peatmoss+perlite 1:1:1(v/v/v)) showed the highest value in all of the characteristics including rooting rate of 68.5% for the effect of growing media on rooting formation.

Cultural Characteristics of Psychrotrophic Lactic Acid Bacteria Isolated from Kimchi (김치에서 분리한 저온성 젖산균의 배양특성)

  • So, Myung-Hwan;Kim, Young-Bae
    • Korean Journal of Food Science and Technology
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    • v.27 no.4
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    • pp.506-515
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    • 1995
  • The cultural characteristics of 60 strains of psychrotrophic lactic acid bacteria which were isolated from kimchi, a Korean traditional fermented vegetable food, and identified as Leuconostoc mesenteroides subsp. mesenteroides, Leu. mesenteroides subsp. dextranicum, Leu. paramesenteroides, Leu. lactis, Lactobacillus bavaricus and Lac. homohiochii were tested. All strains grew at $5^{\circ}C,\;10^{\circ}C\;and\;37^{\circ}C$ in tomato glucose broth, but not at $45^{\circ}C$. The optimum growth temperature of Leu. mesenteroides and Lactobacillus sp. were $33{\sim}34^{\circ}C\;and\;34{\sim}36^{\circ}C$, respectively. All strains of Leu. mesenteroides and Lactobacillus sp. grew at 4.8 and 4.2 of initial pH, but not at 4.0. The final pH of Leu. mesenteroides and Lactobacillus sp. in glucose broth were $3.84{\sim}4.10\;and\;3.82{\sim}3.99$, respectively. None of the 60 strains clotted milk nor reduced litmus in litmus milk. All strains of Leu. mesenteroides and Lactobacillus sp. grew in tomato glucose broth containing 7% ethanol or 6.5% NaCl, but not in the broth containing 15% ethanol or 10% NaCl. All strains grew in tomato glucose broth containing 40% bile juice and survived in the artificial gastric juice of pH 3.5. Furthermore, all strains of Leu. mesenteroides survived in the artificial gastric juice of pH 3.0. Since many strains of lactic acid bacteria tested in this study showed differences in several physiological characteristics from those described in Bergey's Manual of Systematic Bacteriology, it was considered that further tests would be necessary to clarify their positions in taxonomic system.

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Effects of Plant Growth Regulators for In Vitro Propagation of Cannabis sativa varieties (대마 품종별 기내 증식을 위한 식물생장조절제 처리 효과)

  • Hyunseok Lee
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2022.09a
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    • pp.124-124
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    • 2022
  • 대마(Cannabis sativa)는 카나비노이드(cannabinoids) 계열의 성분으로 인한 약리효능 뿐만 아니라 섬유, 식품 등의 분야에서도 식물소재로 활용도가 높은 것으로 알려져 있다. 최근 의료용 대마의 경우 규제완화가 예정됨에 따라 관련 산업분야에서도 안정적 원료 소재 생산에 대한 연구가 요구되고 있다. 대마는 실생번식이 가능한 종이나, 의료용 등 소재의 안정적 공급을 위해서는 균일한 cannabidiol(CBD) 성분의 확보가 가능한 영양증식 방법이 대안으로 사용될 수 있다. 따라서 본 연구에서는 영양증식 방법 중 조직배양 기술을 이용한 대마 품종별(Cherry Blossom, Queen Dream과 Hot Blonde) 증식법 구명을 위하여 식물생장조절제의 종류 및 농도에 따른 기내생육특성을 조사하였다. 세 품종 모두 1 mg l-1 thidiazuron (TDZ) 단독처리 시 신초(> 2cm)가 가장 많이 유도되었다. 단 Queen Dream 품종은 0.5 mg l-1 와 TDZ 0.2 mg l-1 NAA 혼용처리구에서 많은 수의 신초가 유도되었으나 상대적으로 신초의 길이(< 2cm)는 짧은 것으로 확인할 수 있었다. 발생된 신초는 MS 기본배지에서 발근을 유도한 후 토양에 순화하여 정상적인 식물체로 발달되었다. 이와 같은 식물생장조절제 처리를 포함한 식물조직배양 방법은 유용 개체·품종의 증식 및 의료·산업용 원료 소재의 안정적 생산에 활용될 수 있다.

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Quality Characteristics and Anti-proliferative Effects of Dropwort Extracts Fermented with Fructooligosaccarides on HepG2 Cells (미나리 프락토올리고당 발효액의 발효기간에 따른 품질특성 및 간암세포 증식 억제 효과)

  • Kim, Min-Ju;Yang, Seun-Ah;Park, Jung-Hyun;Kim, Hyeok-Il;Lee, Sam-Pin
    • Korean Journal of Food Science and Technology
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    • v.43 no.4
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    • pp.432-437
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    • 2011
  • The quality characteristics and effects on the proliferation of human hepatoma HepG2 cells due to dropwort (Oenanthe javanica) extracts naturally fermented with fructooligosaccharides were investigated. Dropwort was fermented by steeping with the same weight of oligosaccaride at room temperature for 1 year, and then stored at 4$^{\circ}C$ for 1 or 2 more years. During the fermentation periods, total flavonoid content, Hunter's color (a value), and viable cell counts decreased, but reducing sugars including glucose and fructose increased. HepG2 cell proliferation was inhibited significantly by the three extracts, but no effects were observed on Chang cells. In particular, the dropwort extract fermented for 1 year showed the highest inhibitory effect. These results demonstrate that the quality characteristics and anti-proliferative effects of dropwort were affected by fermentation period. It is concluded that dropwort extract fermented for 1 year showed the highest functional properties and quality.

해류제어 막구조 설치해역의 유동구조 특성(1)

  • 김현주;최학선;박용주;박병수
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • 2000.05a
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    • pp.218-219
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    • 2000
  • 바다목장화는 자연급이형 재배어업시스템이며, 해류제어 막구조물(Fig. 1)은 (1)어패류의 서식환경 조성 및 제공, (2)기초생산의 증대를 통한 고차 소비자의 위집과 생산 증대 및 (3)어류의 사료가 되는 저서생물의 증식 효과라는 직접적인 효과와 (4) 와류, 상승류에 수반된 유동변화, 음향발생 등에 의한 부차적인 집어효과를 가질 뿐 아니라 (5) 저층 영양염의 분산 소모를 통한 계절적 영양염의 집중부상에 의한 계절적 부영양화의 감소효과를 가지는 기능시설로서 중요한 증식시설이다(김과 류, 1997). (중략)

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