• Journal of Marine Life Science
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• v.7 no.1
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• pp.29-36
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• 2022

Sea cucumber, Aposticopus japonicus, is a major invertebrate species in the coastal regions of Korea. To evaluate the short-term stress levels according to the releasing methods, this study investigated the gene expression profiles of heat shock protein 90 (HSP90) by real-time quantitative polymerase chain reaction. When the juvenile sea cucumbers were packed in the vinyl bag with oxygen followed by transportation for 30 min or air-exposed for 1 h, the HSP90 gene expression levels in the experimental groups were significantly increased compared to those of the control groups (transported group, p=0.001; air-exposed group, p=0.032). The experimental group at 6 h post-release by seed-spreading method and at 2~6 h post-release by underwater hose-releasing method on board a fishing boat showed that the levels of HSP90 gene expression were not statistically significant but decreased slightly compared to the control group (seed-spreading group, p=0.069; hose-releasing group, p=0.093). On the other hand, the HSP90 gene expression showed an increasing pattern as the time passed (~6 h) after underwater release of juvenile sea cucumbers by divers (p=0.061). These results suggest that HSP90 gene expression can be used to investigate short-term stress response and effective releasing methods of juvenile sea cucumbers.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.56 no.3
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• pp.561-565
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• 2007

We propose glass and PDMS (polydimethylsiloxane) chips for DNA amplification with continuous-flow PCR (polymerase chain reaction). The PDMS microchannel was fabricated using a negative molding method for sample injection. Three heaters and sensors of ITO (indium-tin-oxide) thin films were fabricated on glass chip. ITO heaters and sensors were calibrated accurately for the temperature control of the liquid flow. ITO heater generated stable heat versus applied power. ITO sensor resistance was changed linearly versus temperature increase as a RTD (resistance temperature detector) sensor. As a result, we enable precision temperature control of continuous-flow PCR chip. Using the continuous-flow PCR chip DNA plasmid pKS-GFP 720 bp was successfully amplified.

• Proceedings of the Korea Information Processing Society Conference
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• 2001.10b
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• pp.1151-1154
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• 2001

외판원 문제(Traveling Salesman Problem)는 주어진 n개의 도시들과 그 도시들간의 거리비용이 주어졌을 때, 모든 도시들을 정확히 한번씩만 방문하면서 걸린 비용이 최소가 드는 경로를 찾는 문제로 최적해(optimal)을 구하는 것은 전형적인 NP-완전 문제중의 하나이다. 따라서 외판원 문제를 해결하는 다양한 알고리즘들이 개발되고 있다. 특히 요즈음은 실제 생체 분자(bio-molecule)를 계산의 도구로 사용하는 새로운 계산 방법인 DNA 컴퓨팅은 DNA 분자가 잠재적으로 가지고 있는 막대한 병렬성을 이용해서 NP-완전 문제들을 해결하고자 하는 연구들이 땀이 진행되고 있다. 그러나 아직 실제 생체 분자의 특성을 잘 반영하는 계산 모델이나 분자 생물학에서 사용하는 연산들이 많이 개발되지 알아 계산 효율이 비교적 좋지 않다. 따라서 본 논문에서는 외판원 문제를 해결하기 위한 DAN컴퓨팅의 새로운 중합 효소 연쇄 반응(Polymerase Chain Reaction, PCR) 연산을 개발하였다.

• Korean Journal of Microbiology
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• v.46 no.4
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• pp.405-408
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• 2010

Diversity of myxobacteria in five soil samples from Asansi and Uponeup in Korea was explored by means of polymerase chain reaction (PCR) using primers that specifically bind 16S rDNA of myxobacteria. DNA sequence analysis of 76 PCR fragments containing myxobacterial 16S rDNA revealed five putative novel myxobacterial genera whose 16S rDNA sequences shared <95% sequence identity with those of the type strains. This finding indicates the presence of many uncultured and unidentified myxobacterial species in Korean soil.

• Journal of the Korean Chemical Society
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• v.52 no.6
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• pp.630-636
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• 2008

• Journal of Institute of Control, Robotics and Systems
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• v.12 no.5
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• pp.419-424
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• 2006

In this study, a thermal control system which applied a Peltier device for the polymerase chain reaction(PCR) machine is to be designed. Here in order for it to easily follow the characteristics of the thermal cycle existing for gene amplification of the PCR sample, a PCR control board utilizing a thermal sensor, a Peltier, and a 8 bit microprocessor is made up. Especially a fuzzy type PD control algorithm is applied periodically in time response, and control system is implemented. For that matter, the characteristic data of subject system is obtained and analysed to begin with. Based on this analysed data, the proposed control algorithm is applied and an evaluation of the performance of the whole system take place through the PC.

• Proceedings of the KSME Conference
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• 2004.04a
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• pp.210-215
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• 2004

: A DNA analysis system based on fluorescence analysis has to have a DNA amplifying thermal cycle system. DNA amplification is executed by the temperature control. Accuracy of fluorescence analysis is influenced by the temperature control technology. For that reason, the temperature control is core technology in developing the DNA analysis system. Therefore, the objective of this paper is to develop the hardware to apply thermoelectric module to the DNA amplifying thermal cycle system. In order to verify the developed hardware for controlling the temperature of thermoelectric module, a DNA amplifying thermal cycle test was performed. From the test, the developed hardware controlled the temperature of thermoelectric module successfully. Therefore, it is expected that the developed hardware can be applied to the DNA amplifying thermal cycle system.

• Journal of Veterinary Clinics
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• v.15 no.1
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• pp.140-145
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• 1998

The present study was carried out for the rapid and accurate detection of Anaplasma marginale in cattle using Polymerase Chain Reaction. One pair of primer, BAP-2 and AL34S, were designed to amplify a 409 Up fragment of the A marginale membrane surface protein encoding beta($msp{\beta}l$) gene with a hilly sensitive and specific PCR. A marginale isolated from naturally infected calf in Chonbuk area were used to obtain target genomic DNA for PCR. This study showed that a 409 bp of $msp{\beta}l$ gene fragment could be detected as little as 15 fg of purified A marginale genomic DNA. The amplified fragment with PCR was checked for the identification of $msp{\beta}l$ gene by enzyme restriction and sequencing. Also, the target DNA extracted directly from blood were used in the PCR reactions without prior purification to shorten the detection time. The PCR in the present study was considered convenient and rapid method for the detection of A marginale in whole blood of infected cattle.

• Journal of the Korea Institute of Military Science and Technology
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• v.22 no.4
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• pp.575-580
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• 2019

In biological warfare, it is important to identify biological agents for proper treatment. We focused on developing a real-time RT-PCR kit that can detect multiple species of biological agents. AccuPower(R) Biothreat Real-Time RT-PCR Kit(v3.0) could detect Bacillus anthracis, Yersinia pestis, Vibrio cholerae, Francisella tularensis, Salmonella typhi, Rickettsia prowazekii, Variola virus, Hantaan virus, Yellow fever virus, Brucella spp., Shigella dysenteriae in a single reaction. The results showed that the kit was verified to be able to detect at least 0.005 ng of nucleotide and 10,000 CFU/ml of bacteria. Therefore, the kit is expected to be used as a rapid and sensitive detection kit for 11 species of biological agents within 2 hours.

• Korean Journal of Microbiology
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• v.41 no.4
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• pp.306-311
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• 2005

In this study, the amino-terminal half truncated lump and the whole lump genes from Photobacterium phosphoreum coding for the lumazine protein were cloned by polymerase chain reaction and expressed in Escherichia coli. To identifiy of the binding site of the ligand or substrate, the amino acid identities from the sequences of the lumazine protein, yellow fluorescent protein, and riboflavin synthase from different organisms were also compared and analyzed.