Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Rapid detection of Anaplasma marginale with the Polymerase Chain Reaction in Cattle

중합효소연쇄반응을 이용한 소에 감염된 Anaplasma marginale의 신속한 진단

  • Published : 1998.03.01
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Abstract

The present study was carried out for the rapid and accurate detection of Anaplasma marginale in cattle using Polymerase Chain Reaction. One pair of primer, BAP-2 and AL34S, were designed to amplify a 409 Up fragment of the A marginale membrane surface protein encoding beta($msp{\beta}l$) gene with a hilly sensitive and specific PCR. A marginale isolated from naturally infected calf in Chonbuk area were used to obtain target genomic DNA for PCR. This study showed that a 409 bp of $msp{\beta}l$ gene fragment could be detected as little as 15 fg of purified A marginale genomic DNA. The amplified fragment with PCR was checked for the identification of $msp{\beta}l$ gene by enzyme restriction and sequencing. Also, the target DNA extracted directly from blood were used in the PCR reactions without prior purification to shorten the detection time. The PCR in the present study was considered convenient and rapid method for the detection of A marginale in whole blood of infected cattle.

Keywords

References

  1. J Dairy Sci v.59 Anaplasmosis McCallon BR
  2. Am J Vet Res v.170 Evaluation of free roaming mule deer as carriers of anaplasmosis in an area of Idaho where bovine anaplasmosis is enzootic Renshaw HW;Vaughn HW;Magonigle(et al.)
  3. Am J Vet Res v.45 no.3 Bovine anaplasmosis: In utero transmission and the immunologic significance of ingested colostral antibodies Zaugg JL;Kuttler KL
  4. Am J Vet Res v.46 no.3 Bovine anaplasmosis: Transplacental transmission as it relates to stage of gestation Zaugg JL
  5. Am J Vet Res v.48 no.1 Ovine anaplasmosis: In utero transmission as it relates to stage of gestation Zaugg JL
  6. Schalm's Veterinary Hematology(4th ed.) Hemolytic anemias associated with some infectious agents Jain NC
  7. Veterinary Medicine(8th ed.) Anaplasmosis Radostits OM;Blood DC;Gay CC
  8. JAVMA v.190 Bovine anaplasmosis: Susceptibility of seronegative cows from an infected herd to experimental infection with Anaplasma marginale Lincoln SD;Zaugg JL;Maas J
  9. Essentials of Veterinary Hematology Hemolytic anemias associated with some infectious agents Jain NC
  10. Am J Vet Res v.27 Clinical and hematologic comparision of Anaplasma marginale and Anaplasma centrale infections in cattle Kuttler KL
  11. Am J Vet Res v.27 The comparative susceptibility of calves and adult cattle to bovine anaplasmosis Roby TO;Gates DW;Mott LO
  12. 농시보고 v.12 아나프라스마병에 관한 혈청학적 분포조사 전영;한태우
  13. 대한수의학회지 v.18 no.1 아나플라즈마병에 관한 연구 전영
  14. 대한수의학회지 v.37 no.4 겨울철에 발생한 Holstein 송아지의 Anaplasma marginale 감염 이주묵;권오덕;송희종(등)
  15. 경북대학교 논문집 v.9 경북지방 산양에 발생한 Anaplasmosis 손제영;이현범;장인호
  16. 대한수의학회지 v.33 no.2 재래산양과 호주산 산양에서의 Anaplasmosis 발생보고 백병걸;최인혁;박강희(등)
  17. Infect Immun v.59 no.3 The msp1 beta multigene family of Anaplasma marginale: nucleotide sequence analysis of an expressed copy Barbet AF;Allred DR
  18. J Med Entomol v.30 no.4 Detection of Anaplasma marginale (Rickettsiales: Anaplasmataceae) in secretagogue-induced oral secretions of Dermacentor andersoni(Acari: Ixodidae) with the polymerase chain reaction Stich RW;Sauer JR;Bantle JA(et al.)
  19. J Med Entomol v.30 no.4 Detection of Anaplasma marginale (Rickettsiales: Anaplasmataceae) in hemolymph of Dermacentor andersoni (Acari: Ixodidae) with the polymerase chain reaction Stich RW;Bantle JA;Kocan KM(et al.)
  20. J Vet Diagn Invest v.7 Detection of Anaplasma margnale DNA in bovine erythrocytes by slot-blot and in situ hybridization with a PCR-mediated digoxigenin-labeled DNA probe Ge NL;Kocan KM;Murphy GL(et al.)
  21. Int J Parasitol v.26 no.10 Anaplasma marginale: detection of carrier cattle by PCR-ELISA Gale KR;Dimmock CM;Gartside M;Leatch G
  22. Molecular Cloning, a Laboratory Manual(2nd ed.) Sambrook J;Fritsch EF;Maniatis T
  23. molecular cloning a laboratory manual(2nd Ed.) Maniatis, T.;Fritsch, E.F.;Sambrook, J.
  24. Pro Natl Acad Sci v.74 DNA sequencing with chain termination inhibitor Sanger F;Nicklen S;Coulson AR
  25. PCR protocols: A guide to methods and applications Sample preparation from blood, cells, and other fluids Kawasaki ES
  26. 한국수의공중보건학회지 v.6 도입우의 진드기매개 주혈원충 감염상과 Theileria sergenti의 치료예방에 관한 연구 서명득;장두환
  27. 대한수의학회지 v.27 젖소의 파이로프라스마증의 효과적인 집단검색과 치료방법에 관한 연구 이주묵;김명철
  28. 한국수의공중보건학회지 v.13 no.3 한우에 있어서의 Anaplasma marginale의 미세구조 백병걸;김진호;진찬문(등)
  29. Science v.239 Prime-directed enzymatic amplification of DNA with a thermostable DNA polymerase Saiki RK;Gelfand DH;Stoffel S(et al.)