• Title/Summary/Keyword: 중성적

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Antiadipogenic Activity of Solvent-partitioned Fractions from Limonium tetragonum in 3T3-L1 Preadipocytes (갯질경이 용매분획물의 3T3-L1전지방세포에서의 지방생성억제 효과)

  • Kwon, Myeong Sook;Kim, Jung-Ae;Oh, Jung Hwan;Karadeniz, Fatih;Lee, Jung Im;Seo, Youngwan;Kong, Chang-Suk
    • Journal of Life Science
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    • v.29 no.1
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    • pp.60-68
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    • 2019
  • Limonium tetragonum, an edible halophyte that grows on salt marshes in Korea, is thought to possess various health benefits (e.g., antioxidant, antitumor, and hepatoprotective). In the present study, different solvent partitioned subfractions, water ($H_2O$), buthanol (n-BuOH), 85% aqueous methanol (85% aq. MeOH), and hexane (n-hexane), from crude extract of L. tetragonum were tested for their ability to prevent adipogenesis in differentiating 3T3-L1 preadipocytes. The treatment of differentiating 3T3-L1 preadipocytes with L. tetragonum subfractions (LTFs) resulted in suppressed adipogenesis and reduced expression of adipogenesis-related transcription factors such as peroxisome proliferator-activated receptor gamma ($PPAR{\gamma}$), CCAATT/enhancer-binding protein alpha ($C/EBP{\alpha}$), and sterol regulatory element-binding protein 1c (SREBP-1c) at both mRNA and protein levels. In addition, the LTF treatment notably decreased the levels of phosphorylated p38, extracellular signal-regulated kinase (ERK), and c-Jun N-terminal kinase (JNK) of the mitogen-activated protein kinase (MAPK) pathway in association with $PPAR{\gamma}$-linked adipogenesis. Among all the tested LTFs, $H_2O$ and n-hexane were the most effective in lowering lipid accumulation and regulating the adipocyte differentiation via $PPAR{\gamma}$ pathway. Taken together, the results indicated that the $H_2O$ and n-hexane LTFs contain bioactive compounds that may exhibit significant antiadipogenesis activity by downregulation of the $PPAR{\gamma}$ pathway and inactivation of the MAPK signal pathway in 3T3-L1 preadipocytes.

Ethanol Extract of Hermetia illucens Larvae Inhibits Adipogenesis in 3T3-L1 Adipocytes (동애등에 유충 에탄올 추출물의 지방세포 분화 억제 효과)

  • Park, Ji Yeong;Kwak, Kyu-Won;Choi, Ji-Yeon;Lee, Si-Eun;Kim, Yong-Soon;Koo, Bonwoo;Kim, Eunsun;Park, Kwanho;Kim, Sun Young
    • Journal of Life Science
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    • v.31 no.12
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    • pp.1094-1099
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    • 2021
  • Hermetia illucens (Black soldier fly) is attracting attention as an environmental purification insect because it can supply a wide range of by-products of the agricultural food industry. Also, it has a potential feed for fish, birds, and pets due to a short life cycle and excellent nutritional components. Several pharmacological effects, including antimicrobial, of H. illucens have been reported. However, no study has focused on antiobesity effects of ethanol extract of H. illucens. In this study, we aimed to assess the anti-obesity effects of ethanol extract of H. illucens larvae (HIE) through inhibition of differentiation of 3T3-L1 preadipocytes into adipocytes. The amount of lipid accumulated in adipocytes was measured by oil red-O staining, and the inhibitory effect on adipogenesis was confirmed. The expression levels of factors related to adipocyte differentiation and fat synthesis were determined using Western blot analysis. Lipid droplet formation in adipocytes was remarkably inhibited by HIE. In addition, treatment with 400 ㎍/ml of HIE significantly reduced the expression levels of peroxisome proliferator-activated receptor γ and CCAAT/enhancer-binding protein α-transcription factors involved in adipocyte differentiation. Therefore, the results of this study indicate that HIE is a potential anti-obesity agent because it inhibits adipocyte differentiation.

Scientific Analysis and Conservation Treatment on the Buddhist Scriptures of Paper Relics Excavated from Sum Tolgoi, Mongolia (몽골 숨 톨고이 출토 지류 유물의 과학적 분석 및 보존처리)

  • Bae, Su Bin;Yang, Min Jeong;Kwon, Yun Mi;Yoo, Ji Hyun;Jeong, Hee Won
    • Journal of Conservation Science
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    • v.37 no.6
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    • pp.723-737
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    • 2021
  • This study analyzed the composition and structure of materials with Buddhist paper scriptures excavated from architectural sites in 'Sum Tolgoi' of the 17th century and carried out conservation treatment base on the result of the analysis. The scriptures were covered in dust and foreign sub stances, and were so crumpled that it was impossible to identify the form. The damage, loss, and discoloration have been identified. Buddhist scriptures written in Tibetan used indigo and ink sticks on paper as a result of UV-Vis analysis, and ink sticks as black character materials from scriptures written ancient Mongolian. SEM-EDS and Micro-XRF analyses revealed that the outlines were drawn with red lines using a mix of Minium (Pb3O4) and Cinnabar (HgS), or Cinnabar (HgS) alone, and the contents of the scriptures were written with silver paint. Silver chloride (AgCl) and Calcium (Ca) were identified in the silver paint component of the characters, while Calcium and Orpiment (As2S3) were identified in the yellow lines. Concerning the paper ground, Buddhist scriptures written in ancient Mongolian were characterized by herbal plant fiber and bast fiber, and those written in Tibetan, by bast fiber. Radiocarbon dating indicates that the paper for the scriptures was produced between the 15th and 17th centuries. Conservation treatment of the scriptures was carried out based on the experiment on the production of pre-coated paper and how to coat that to prevent the second damage due to the deformation and fragility of the excavated paper. The scriptures were preserved and mounted, and a neutral box was made to identify the contents of the scriptures recorded on both sides after the treatment. This conservation treatment is the result of a study that applied new conservation treatment materials and methods according to the principle of conservation treatment reversibility.

Evaluation of Growth Characteristics and Yield Potential of Summer Emergency Forage Crops (하계 응급 조사료 자원의 생육특성 및 조사료 생산성 평가)

  • Park, Hyung Soo;Choi, Ki Choon;Yang, Seung Hak;Jung, Jeong Sung;Lee, Bae Hun
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.42 no.1
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    • pp.26-31
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    • 2022
  • This study was carried out to evaluate the growth characteristics and forage yield potential for warm season grass as emergency forages. The experimental design was a randomized block design (RBD) with three replications. Two barnyard millet (Echinochloa species cv. Shirohie and Jeju native), a pearl millet (Pennisetum glaucum cv Feed milk 2) a proso millet (Panicum miliaceum cv Native), a teffgrass (Eragrostis tef cv. Tiffany) and a kleingrass (Panicum coloratum cv. Selection 75) were compared for forage production and quality at the Mid regions of Korea. Warm season forage crops were sown on May 21 and June 23 respectively, and in 2021, it was sown twice on May 21 and June 21 The number of days to seedling emergence for barnyard millet and teffgrass was observed approximately 10 and 3 days after seeding, respectively. The cultivation period from seeding to harvest was within 60 days for all entry spices except for the late-heading type barnyard millet (within 84 days). As for the dry matter yield by seeding date, the dry matter yield of the late-heading type barnyard millet in May seeding was the highest at 23,872 kg/ha, and the kleingrass was the lowest at 3,888 kg/ha. For the June seeding, the dry matter yield of the late-heading type barnyard millet was 17,032 kg/ha, the highest, and the proso millet, teffgrass and kleingrass showed the lowest at 5,468, 5,442, and 5,197 kg/ha, respectively. The crude protein (CP) content was varied by warm season grass species, but the early-heading type barnyard millet, teffgrass, and kleingrass showed the highest tendency, and the late-heading type barnyard millet showed the lowest at 5.7~5.9%. Neutral detergent fiber (NDF) and acid detergent fiber (ADF) content did not show a significant difference between the seeding in May, but kleingrass in June sowed lower than the others.

A STUDY ON THE IONOSPHERE AND THERMOSPHERE INTERACTION BASED ON NCAR-TIEGCM: DEPENDENCE OF THE INTERPLANETARY MAGNETIC FIELD (IMF) ON THE MOMENTUM FORCING IN THE HIGH-LATITUDE LOWER THERMOSPHERE (NCAR-TIEGCM을 이용한 이온권과 열권의 상호작용 연구: 행성간 자기장(IMF)에 따른 고위도 하부 열권의 운동량 강제에 대한 연구)

  • Kwak, Young-Sil;Richmond, Arthur D.;Ahn, Byung-Ho;Won, Young-In
    • Journal of Astronomy and Space Sciences
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    • v.22 no.2
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    • pp.147-174
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    • 2005
  • To understand the physical processes that control the high-latitude lower thermospheric dynamics, we quantify the forces that are mainly responsible for maintaining the high-latitude lower thermospheric wind system with the aid of the National Center for Atmospheric Research Thermosphere-Ionosphere Electrodynamics General Circulation Model (NCAR-TIEGCM). Momentum forcing is statistically analyzed in magnetic coordinates, and its behavior with respect to the magnitude and orientation of the interplanetary magnetic field (IMF) is further examined. By subtracting the values with zero IMF from those with non-zero IMF, we obtained the difference winds and forces in the high-latitude 1ower thermosphere(<180 km). They show a simple structure over the polar cap and auroral regions for positive($B_y$ > 0.8|$\overline{B}_z$ |) or negative($B_y$ < -0.8|$\overline{B}_z$|) IMF-$\overline{B}_y$ conditions, with maximum values appearing around -80$^{\circ}$ magnetic latitude. Difference winds and difference forces for negative and positive $\overline{B}_y$ have an opposite sign and similar strength each other. For positive($B_z$ > 0.3125|$\overline{B}_y$|) or negative($B_z$ < -0.3125|$\overline{B}_y$|) IMF-$\overline{B}_z$ conditions the difference winds and difference forces are noted to subauroral latitudes. Difference winds and difference forces for negative $\overline{B}_z$ have an opposite sign to positive $\overline{B}_z$ condition. Those for negative $\overline{B}_z$ are stronger than those for positive indicating that negative $\overline{B}_z$ has a stronger effect on the winds and momentum forces than does positive $\overline{B}_z$ At higher altitudes(>125 km) the primary forces that determine the variations of tile neutral winds are the pressure gradient, Coriolis and rotational Pedersen ion drag forces; however, at various locations and times significant contributions can be made by the horizontal advection force. On the other hand, at lower altitudes(108-125 km) the pressure gradient, Coriolis and non-rotational Hall ion drag forces determine the variations of the neutral winds. At lower altitudes(<108 km) it tends to generate a geostrophic motion with the balance between the pressure gradient and Coriolis forces. The northward component of IMF By-dependent average momentum forces act more significantly on the neutral motion except for the ion drag. At lower altitudes(108-425 km) for negative IMF-$\overline{B}_y$ condition the ion drag force tends to generate a warm clockwise circulation with downward vertical motion associated with the adiabatic compress heating in the polar cap region. For positive IMF-$\overline{B}_y$ condition it tends to generate a cold anticlockwise circulation with upward vertical motion associated with the adiabatic expansion cooling in the polar cap region. For negative IMF-$\overline{B}_z$ the ion drag force tends to generate a cold anticlockwise circulation with upward vertical motion in the dawn sector. For positive IMF-$\overline{B}_z$ it tends to generate a warm clockwise circulation with downward vertical motion in the dawn sector.

Analysis of Dietary Habits by MDA(Mini Dietary Assessment) Scores and Physical Development and Blood Parameters in Female College Students in Seoul Area (서울 지역 여대생의 식생활 평가에 따른 식습관, 신체 발달 및 혈액 인자 비교 연구)

  • Choi, Kyung-Soon;Shin, Kyung-Ok;Huh, Seon-Min;Chung, Keun-Hee
    • Journal of the East Asian Society of Dietary Life
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    • v.19 no.6
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    • pp.856-868
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    • 2009
  • This study was conducted to investigate causes for health problems among college women by analyzing factors related to their dietary habits, physical development, health habits, and blood parameters. The subjects were ages 20 to 24 years, lived in the Seoul area and were randomly selected during March, 2008 to August, 2009. The average height and weight of the overall subjects were $162.02{\pm}4.89\;cm$ and $53.96{\pm}7.00\;kg$, respectively. According to a 3-point assessment scale for the subjects' dietary habits, the average point value was 21.2. The percentage of subjects that ate breakfast daily was only 30.5%, and they omitted regular meals at least once a week. Approximately 83.5% of the subjects reported eating out often or frequently, and preferred Korean foods when they ate out. The subjects had interim meals (snacks) one or two times daily, and 40.4% of them preferred unbalanced meals. As their interim meals, among the 'good' group, ate breaded potatoes (39.3%), carbonated beverages, and ice cream (36.8%), whereas the 'poor' group, drank milk and ate dairy products (38.0%) as well as fast food and fried food (22.8%). Intakes of energy, fat, vitamins $B_2$ and $B_6$, niacin, folic acid, calcium, iron, zinc, and phosphorus were higher in the 'poor' group. The average hemoglobin level ($13.77{\pm}1.00\;g/dL$) among the subjects was within normal range; while 2.7% of subjects had hemoglobin levels under 11.1 g/dL (standard value) and were examined as anemic. The degree of interest in health was 24.5% higher among the subjects who had poor dietary habits. In contrast, among those who had good dietary habits, 49.6% reported they had no interest in regular exercise. The subjects reported that regular meals, nutrient intake, sufficient rest, and sleep as necessary to maintain health. The average amount of sleep obtained by the subjects was 6~8 hours. Among the 'poor' group, 36.2% reported that they exercised regularly, whereas 18.5% of the subjects in the 'good' group reported regular exercise (p<0.05). In conclusion, it appears necessary to provide nutrition education through teaching and to promote nutrition and health to college women so they can control their individual health status and create practicable dietary plans.

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Exploration and Development in the Janggun Pb-Zn Mine (장군광산(將軍鑛山)의 탐사(探査)와 개발현황(開發現況))

  • Kho, Suck Jin
    • Economic and Environmental Geology
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    • v.20 no.4
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    • pp.289-303
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    • 1987
  • 당(當) 광산(鑛山)은 1936년(年) 금(金), 은(銀) 광종(鑛種)으로 출원(出願)하였다가 1940년(年) 망간을 추가(追加)하여 망간 광산(鑛山)으로 1975년(年)까지 Mn(30~35%) 110,000여(餘)톤을 생산(生産), 국내생산량(國內生産量)의 70%를 점(占)하였고 1976년(年) Mn광상(鑛床) 하부(下部)에 연(鉛), 아연(亞鉛) 유화광(硫化鑛)을 발견(發見), 현재(現在)까지 Pb十Zn=10% 이상(以上) 원광석(原鑛石) 500,000여(餘)톤을 처리(處理), 연정광(鉛精鑛)(Pb : 62%) 37,000여(餘)톤, 아연정광(亞鉛精鑛)(Zn : 46.5%) 37,000여(餘)톤, 유비광정광(硫砒鑛精鑛)(As : 30%) 5,000여(餘)톤을 생산(生産)하였다. 현재(現在) 일처리(日處理) 220톤 선광장(選鑛場)을 일처리(日處理) 400톤 규모(規模)로 증설계획중(增設計劃中)이다. 당(當) 광산(鑛山)에서 현재(現在)까지 시행(施行)한 갱외시추(坑外試錐)는 75개공(個孔) 18,500여(餘)m, 갱내시추(坑內試錐) 750개공(個孔) 40,000여(餘)m 갱도(坑道) 총연장(總延長) 13,000m에 달(達)하며 지표(地表)(623ML)로 부터 수직(垂直) 300m 하부(下部)까지 갱도(坑道)가 개착(開鑿)되어 있다. 당(當) 광산(鑛山)의 지질(地質)은 여러 조사서(調査書)에 의(依)하여 견해(見解) 차이(差異)를 보여주고 있으나 대체(大體)로 다음과 같은 쪽으로 인정되고 있다. 즉(卽) 본지역(本地域) 루층군(累層群)의 층순(層順)을 하위(下位)로 부터 상위(上位)로 향(向)하여 원남층(遠南層)${\rightarrow}$율리통(栗里統)${\rightarrow}$장산규암층(壯山珪岩層)${\rightarrow}$두음리층(斗音里層)${\rightarrow}$장군석회암층(將軍石灰岩層)${\rightarrow}$동수곡층(東水谷層)${\rightarrow}$재산층(才山層)의 순위(順位)로 보며 장산규암층(壯山珪岩層)과 두음리층(斗音里層)을 조선계(朝鮮系)의 양덕통(陽德統)으로, 장군석회암층(將軍石灰岩層)을 대석회암통(大石灰岩統)으로, 동수곡층(東水谷層)과 함탄층(含炭層)인 재산층(才山層)을 평안계(平安系) 지층(地層)으로 대비(對比)한다. 이들은 본지역(本地域) 북(北)쪽에서는 선(先)캠브리아기(紀)의 원남층(遠南層)과 율리통(栗里統)을 불정합(不整合)으로 덮고 남측(南側)에서는 재산층(才山層)과 원남층(遠南層)이 단층접촉(斷層接觸)하고 있다. 이들 지층(地層)의 주향(走向)은 $N60^{\circ}{\sim}80^{\circ}W$, $N60^{\circ}{\sim}80^{\circ}E$이며 경사(傾斜)는 대체(大體)로 $50^{\circ}{\sim}80^{\circ}N$이며 전체적(全體的)으로 역전(逆轉)된 층서(層序)를 보여주는 바 지질구조(地質構造)에 있어서 단사구조(單斜構造)인지 등사(等斜)습곡의 향사(向斜), 또는 등사(等斜)습곡이 배사구조(背斜構造)인지 아직 밝혀지지 않고 있다. 화성암체(火成岩體)는 본지역(本地域) 서측(西側)에 쥬라기(紀) 춘양화강암(春陽花崗岩)이 불규칙(不規則)한 실입(實入) 접촉면(接觸面)을 보여주며 시대미상(時代未詳)(백악기(白堊紀)?)의 거정화강암(巨晶花崗岩), 반화강암(半花崗岩)이 소암주상(小岩株狀)으로 몇 곳 실입(實入)하고 산성(酸性)~중성(中性)의 맥암(脈岩)과 염기성(鹽基性) 안산암질암(安山岩質岩)이 실입(實入)해 있다. 광상(鑛床)은 장군석회암층(將軍石灰岩層)에 배태(胚胎)되어 있는 열수교대(熱水交代) 연(鉛), 아연(亞鉛), 은등(銀等)의 혼합(混合) 유화광상(硫化鑛床)으로 다량(多量)의 Mn분(分)을 수반(隨伴)하며 지표부(地表部)에 Mn광상(鑛床)을 형성(形成)하고 있다. 광상(鑛床)의 형태(形態)는 괴상(塊狀), 각력(角礫)pipe상(狀), 맥상(脈狀)으로 나타난다. 광상(鑛床)의 성인(成因)과 생성시기(生成時期)에 대(對)하여 많은 논란(論難)이 있다. 즉(卽) 열수교대(熱水交代)냐, 접촉교대(接觸交代)냐, 동시퇴적기원(同時堆積起源)이냐, 또는 생성시기(生成時期)가 쥬라기(紀)인지 백악기(白堊紀)인지에 대해 이론(異論)이 있다. 본지역(本地域) 광상(鑛床)은 남본(南本), 100우(右), 북(北), 유비철(硫砒鐵), 동(東), 서(西), 재남(才南), 재동(才東), 110호(號) 등(等)이 지표(地表) Mn로두광화대(露頭鑛化帶)와 관련(關聯) 명명(命名)된 바 전(前)4자(者)는 하부(下部)에서 유화광상(硫化鑛床)이 확인(確認)되었으나 나머지 후자(後者)에서는 아직 하부(下部)에 유화광상(硫化鑛床)이 확인(確認)되지 않고 있으며 남본광상(南本鑛床)으로 부터 남동(南東) 300여(餘)m 지점에 장군석회암층(將軍石灰岩層)과 동수곡층(東水谷層) 경계부(境界部)에 Fe 55~60% 자철광상(磁鐵鑛床)이 확인(確認)된 바 신례미(新禮美) 자철광상(磁鐵鑛床)과 유사성(類似性)이 있는 것 같아 흥미(興味)롭다. 당(當) 광산(鑛山)의 현재(現在)까지의 탐광(探鑛)은 남본광상(南本鑛床) 지표로두(地表露頭)(Mn) 하부(下部)에서 확인(確認)된 연(鉛), 아연(亞鉛), 은(銀) 유화광체(硫化鑛體) 하부(下部)와 전탐(電探)에 의(依)해 확인(確認)된 북광체(北鑛體), 갱도접근중(坑道接近中)에 확인(確認)된 100우광체(右鑛體), 유비철광체(硫砒鐵鑛體) 등(等)의 하부(下部) 탐광(探鑛)을 주(主)로 하고 지표(地表) Mn로두(露頭) 하부(下部)에 대(對)한 시추탐광(試錐探鑛0을 병행(竝行)하고 있으며 시추(試錐)에 의(依)해서 지표(地表)로 부터 790m 하부(下部)(해발(海拔) 200ML)까지 광화대(鑛化帶)가 확인(確認)되었다. 향후(向後) 탐광방침(探鑛方針)을 확고(確固)히 수립(樹立)하기 위(爲)하여는 광상(鑛床)의 성인구명(成因究明)은 물론(勿論) 광상(鑛床)의 배태조건(胚胎條件)에 있어 지질구조규제(地質構造規制)와 화강암(花崗岩)의 실입상(實入狀)과의 관계(關係), 광액(鑛液)의 통로(通路)에 대(對)한 지질구조(地質構造), 모암(母岩)의 화학(化學) 물리적(物理的) 특성(特性)에 대(對)한 연구(硏究) 검토(檢討)가 었어야 하겠다.

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Effects of Blended Essential Oil(CRINA®) Supplementation on the Performance, Nutrient Digestibility, Small Intestinal Microflora and Fatty Acid Composition of Meat in Broiler Chickens (사료중 Blended Essential Oil(CRINA®) 첨가가 육계의 생산성과 영양소 이용률, 소장 내 미생물 균총 및 계육내 지방산 조성에 미치는 영향)

  • Suk, J.C.;Lim , H.S.;Paik, I.K.
    • Journal of Animal Science and Technology
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    • v.45 no.5
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    • pp.777-786
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    • 2003
  • An experiment was conducted to investigate the effects of supplementary Blended essential oil(CRINA$^{\circledR}$) on the performance, nutrient availability, fatty acid composition of leg muscle, small intestinal microflora and blood parameters in broiler chickens. One thousand unsexed day-old broiler chickens were assigned to five treatments : control(T1), 5ppm avilamycin(starter diet) & 5ppm flavomycin(grower diet) T2, 5ppm avilamycin(starter diet) & 50ppm CRINA$^{\circledR}$(grower diet) T3, 50ppm CRINA$^{\circledR}$(starter & grower diet) T4, 50ppm CRINA$^{\circledR}$+ 500ppm lactic acid$^{\circledR}$ (starter & grower diet) T5. Each treatment had four replications of 50 birds each. Growth performance was significantly improved by dietary supplements(T2-T5). There were no significant differences among treatment T2, T3, T4 and T5. Feed intake was not significantly different among treatments. Dietary supplementation of CRINA$^{\circledR}$(T3, T4, T5) resulted in significant(p〈0.05) improvement in feed/gain(F/G) during finishing period (4-5weeks). The birds fed CRINA$^{\circledR}$ supplemented diet(T4) showed significantly(p〈0.05) higher availability of crude fat, methionine and methionine + cystine than those fed antibiotics supplemented diet(T2). Mortality was not significantly affected by treatments. The colony forming unit(CFU) of E.coli in small intestinal content was significantly lower in antibiotics & CRINA$^{\circledR}$(T3) compared to CRINA$^{\circledR}$ treatment(T4)(P〈0.05). CFU of Cl. perfringens was low in CRINA$^{\circledR}$(T4) but not different significantly with other treatments. Serum triglyceride level of birds fed CRINA$^{\circledR}$ + lactic acid diet(T5) was significantly lower(p〈0.05) than those fed antibiotics supplemented diet(T2). Cholesterol level of the birds fed antibiotics(T2) or CRINA$^{\circledR}$ + lactic acid supplemented diet(T5) was significantly higher(p〈0.05) than other treatments. HDL level of birds fed control diet was significantly lower(p〈0.05) than that of others. The levels of serum IgG were not significantly different among treatments. Major fatty acids composition of leg muscle fat was significantly influenced by treatments. Control group showed significantly higher palmitic acid(C$_{16:0}$) and steraric acid(C$_{18:0}$) content than other treatments(p〈0.05). Content of oleic acid(C$_{18:1}$), however, was significantly lower in the control than others treatments. Content of linolenic acid(C$_{18:3}$) was significantly higher in CRINA$^{\circledR}$+ lactic acid(T5) than antibiotics & CRINA$^{\circledR}$(T3) treatments. Total saturated fatty acids content was higher and total unsaturated fatty acids were lower in the leg muscle fat of the control than that of other treatments. It is concluded that CRINA$^{\circledR}$ supplementation improved growth rate and F/G ratio in broilers. The combination of CRINA$^{\circledR}$ with either antibiotics or lactic acid did not show any additive or synergistic effects in broiler chickens .

Effect of Dietary Energy, Protein on Growth and Blood Composition of Cross Bred Chicks (유색육용계의 성장과 혈액성상에 사료단백질 및 에너지가 미치는 영향)

  • Jeong, Y.D.;Ryu, K.S.
    • Korean Journal of Poultry Science
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    • v.35 no.3
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    • pp.291-302
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    • 2008
  • To acquire essentially necessary basic data to establish feeding system by verifying appropriate dietary energy and protein level for the growth of commercial slow growing broiler chicks within the country, two experiments were conducted for 5 weeks. One day old, 1,404 male and female broiler chicks were used for the experiments, and 26 chicks were placed at each pen. The energy level of feed was maintained about 3,000 or 3,100 kg/kcal for whole breeding period of 5 weeks, and protein content was adjusted about 20, 21, and 22% during the first two weeks and the content was adjusted to 18, 19, 20, 21, and 22% from the 3 to 5 weeks old of the experiment. The categories of body weight and feed intake amount were monitored to calculate the productivity and blood sampling was conducted for the analysis at the end of each experiment. Experiment 1:Although the productivity by the ME content difference during $0{\sim}2$ weeks did not have significant difference and the body weight increase by the difference of CP content and feed intake amount did not have much difference, the feed requirement rate was statistically improved in CP 21 and 22% treatment groups compared to the CP 20% group (P<0.05). The feed ME 3,100 kcal/kg treated group during $3{\sim}5$ weeks after starting the experiment revealed to show improved feed requirement rate (P<0.05). Within the period of experiment, the CP 22% treated group resulted to show significant body weight increase compared to the groups treated with low levels of CP (P<0.05) and the feed requirement rate was improved in high CP treated group compared to low CP treated groups, but the feed intake amount did not show significant difference between treated groups. During the experiment period, the body weight increase and feed requirement rate revealed to interact between ME and CP (P<0.05). During the whole experiment period of the 5 weeks, the feed requirement rate was improved in ME 3,100 kcal/kg treated group than the groups treated with ME 3,000 kcal/kg, and the CP (20) 18% treatment groups resulted to show higher values than other treatment groups (P<0.05). Body weight increase was high in CP (22) 22% treated groups than those of CP (21) 21% and (20) 18% treated groups, and the interaction between ME and CP was found at body weight increase and feed requirement rate (P<0.05). Although blood albumin and total cholesterol levels were elevated in ME 3,100 kcal/kg treated group than ME 3,000 kcal/kg treated group, but neutral fat content was reduced (P<0.05). On the other hand, the total cholesterol content was increased in CP (22) 21% treated group than CP (22) 20% and CP (20) 18% treated groups (P<0.05). Experiment 2: The body weight increase in 0-2 weeks was higher in ME 3,100 kcal/kg treated group than ME 3,000 kcal/kg treated group, and it was highly improved in CP 22% treated group than CP 20% treated group by showing the interaction between CP and ME (P<0.05). The significant improvement of feed requirement rate was observed in CP 21% and 22% treated groups compared to CP 20% treated group (P<0.05). The productivity between the growth period from 3 to 5 weeks of age and whole growth period resulted to show no significant difference. Although no difference was observed in blood composition between treated groups, the interaction of ME and CP on cholesterol content was accepted at the range of P<0.05). Therefore, it is considered that the appropriate dietary protein level within feed for the physiology of growing broiler chicks was 22% or more for the first two weeks and protein level of 21% or 20% from 3 to 5 weeks old for the maximization of productivity. Even if the energy level within feed had some partial effects on the productivity, but did not show consistency. So, further experiments needto be conducted by differentiating the energy level.