To measure the soil-to-plant transfer factors ($TF_a,\;m^2\;kg^{-1}$-fresh) of radionuclides deposited during the growing season of potato, a radioactive solution containing $^{54}Mn,\;^{60}Co,\;^{85}Sr$ and $^{137}Cs$ was applied to the soil surfaces in soil boxes 2 d before seeding and three different times during the plant growth. For the pre-seeding application (PSA), radionuclides were mixed with the topsoil (loamy sand and 5.2 in pH). The plant parts investigated were leaves, stems, tuber skin and tuber flesh. The $TF_a$ values of $^{54}Mn,\;^{60}Co,\;^{85}Sr$ and $^{137}Cs$ from the PSA were in the ranges of $1.9{\times}10^{-4}{\sim}1.5{\times}10^{-2}$, $1.8{\times}10^{-4}{\sim}7.5{\times}10^{-4}$, $4.0{\times}10^{-4}{\sim}1.6{\times}10^{-2}$, $1.5{\times}10^{-4}{\sim}3.9{\times}10^{-4}$ respectively, for different plant parts. The TFa values from the growing-time applications were on the whole a few times lower than those from the PSA. For $^{54}Mn,\;^{85}Sr$ and $^{137}Cs$, the $TF_a$ values from the early- or middle-growth-stage application were higher than those from the late-growth-stage application, whereas the opposite was true for $^{60}Co$. Leaves and tuber flesh had the highest and lowest $TF_a$ values, respectively, in most cases. The total uptake from soil by the four plant parts was in the range of $0.05{\sim}3.16%$. In the third year following the PSA, the $TF_a$ values of $^{54}Mn,\;^{60}Co$ and $^{137}Cs$ were $11{\sim}25%$, $21{\sim}25%$ and $38{\sim}67%$ of those in the first year, respectively, depending on the plant parts. The present results can be used for estimating the radiological impact of an acute radioactive deposition during the growing season of potato and for testing the validity of relevant food-chain models.
Kim, Do-Kyung;Song, Soo-Keun;Kim, In-Jin;Kook, Joong-Ki
Journal of the Korean Society of Food Science and Nutrition
/
v.33
no.9
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pp.1451-1456
/
2004
Amino acid transporters play an important role in supplying nutrients to normal and cancer cells for cell proliferation. System L is a major transport system responsible for the $Na^+$ -independent, large neutral amino acids including several essential amino acids. L-type amino acid transporter 1 (LAT1), an isoform of system L amino acid transporter, is highly expressed presumably to support their continuous growth and proliferation in malignant tumors. In the present study, we have examined the correlation between the expressions of amino acid transporter LAT1 mRNA and its subunit 4F2hc mRNA and the amount of L-leucine transport in various human cancer cell lines. Northern blot analysis have revealed that the 26 human cancer cell lines expressed LAT1 mRNA and 4F2hc mRNA. There were the differences for the levels of LAT1 and 4F2hc mRNA expressions in the 26 human cancer cell lines. The 26 human cancer cell lines transported the L-[$^{14}C$]leucine into the cells via amino acid transporter. In the 26 human cancer cell lines, a linear relationship was observed between the expression of amino acid transporter LAT1 mRNA and the amount of L-leucine transport. Little relationship was observed between the expression of 4F2hc mRNA and the amount of L-leucine transport, but the statistical significance of difference was not detected. These results indicate that the 26 human cancer cell lines express LAT1 mRNA and 4F2hc mRNA and there is the correlation between the expression of amino acid transporter LAT1 mRNA and the amount of L-leucine transport. In addition, specific inhibition of LAT1 in cancer cells will be a new rationale for anti-cancer therapy.
Journal of the Korean Society of Environmental Restoration Technology
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v.3
no.1
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pp.10-19
/
2000
Due to the urbanization, concentrated population, and limited land exploitation in the modern society, the environment surrounding that we live in is getting polluted more and more, and it has become hard even to let urban children experience the nature. This research was conducted to help people recognize the importance of our natural resources through the environmental education of elementary school and to use school's practical open-space for the Indigenous Plants education. The results of this study are as follows : First, the status of a plant utilization in our institutional education : There were 362 species totally of 124 species of Trees, 156 species of Herbs, 63 species of Crops, and 19 species of Hydrophytes which appear in the elementary school text book. Of all, the most frequently appearing species of tree were the Malus pumila var. dulcissima, Pinus densijlora, Citrus unshiu, Diospyros kaki. Second, the effect of plant education using the land around schools : The result of research on the open-space of the 19 elementary schools located in Mapo-gu showed that most of the species planted are the Juniperus chinensisrose, Hibiscus syriacus. Pelargonium inquinans in the order of size, and the plants appearing in text book were grown in the botanical garden organized in 7 schools. Especially most of the Indigenous Plants were being planted in botanical garden, and Pinus densijlora, Abeliophyllum distichum, Polygonatum var. plurijlorum, Liriope platyphylla and so on. Last, the result of this research on recognition of Environment, Planting education and Indigenous plants : It showed that educational necessity of students and teachers about environment and Indigenous Plants was more than 80%. The management of botanical garden was conducted by some teachers and managers. The results of this study suggested that we needed the reconstruction of curriculum, the efficient application of plant education for effectiveness of using school environment and monitoring continually and construction information sources for the better environment education in the elementary schools.
The Cretaceous and the Upper Jurassic strata of the Korean Peninsula, entirely of continental facies, form a sedimentary mega-unit subdivided into three unconformity-bounded units. The lower, Upper Jurassic-early Lower Cretaceous unit (Jasong Synthem) occurs profusely in North Korea and is characterized by volcanic rocks of intermediate to acidic, calc-alkaline to alkaline compositions; but strata of this unit is very rare in South Korea. The middle, Hauterivian-Lower Albian unit occurs commonly in the Korean Peninsula, but some alkalinesubalkaline basalt and andesite occur only in South Korea. A recently obtained U-Pb isochron age about 113.6 Ma (Chang et at, 1998) from the zircon grains of the Kusandong Tuff in the uppermost part of the Haman Formation has thrown much light on the age of this unit. The stratotype of this Hauterivian-L. Albian unit is the Sindong and Hayang Groups of the Kyongsang Basin, where the unit is about twice thick and has more conglomerates than in sedimentary basins in North Korea. The unit shows various sedimentary cycles in different basins showing that the cyclicity is controlled by local crustal motion. The upper, Upper Albian-Upper Cretaceous unit is abundant in South Korea with prolific volcanic rocks which are intermediate to acidic and notably calc-alkaline. In North Korea, however, this unit occurs in only one locality without volcanic rocks and is not voluminous. The distribution of these three unconformity-bounded units shows a stepwise younging toward the Pacific Ocean: the lower unit occurs mainly in N Korea, the middle unit occurs in both N and S Korea, and the upper unit occurs mainly in the southern part of S Korea. The Cretaceous sedimentary basins of S Korea were genetically controlled by paralleling sinistral strike-slip faults parallel to the Pacific margin.
A paleomagnetic investigation of the Mesozoic Daedong Supergroup and the Precambrian Seosan Group in the Kyeonggi massif is carried out to elucidate the tectonic evolution of Korea under the effect of the collision between Korea and the North/South China Blocks. For the Daedong Supergroup, the characteristic direction of D/I=74.5$^{\circ}$/36.7$^{\circ}$(k=60.7, $\alpha$=5.1$^{\circ}$) after tilt correction is better clustered than that before tilt correction (D/I=61.9$^{\circ}$/52.8$^{\circ}$, k=4.4,$$\alpha$_{95}$=21.5$^{\circ}$), indi-cating that it is a primary magnetization acquired during the formation of the rock. Paleomagnetic pole position of the formation locates at 208.0$^{\circ}$E, 24.5$^{\circ}$N (n=14, K=67.5, $A_{95}$=4.9$^{\circ}$), statistically similar to those of Middle Triassic period of the SCB, revealing that the two had occupied the same tectonic unit during this period. It is observed that only 6 out of 33 sites of the Seosan Group yield remagnetized paleomagnetic direction. The rest of the sampling sites reveals severe dispersion of magnetic directions presumably due to the consequences of the collision between Korea and the North/South China Blocks. The characteristic direction of the Seosan Group is D/I=45.7$^{\circ}$/60.1$^{\circ}$(k=41.2,$$\alpha$_{95}$=10.6$^{\circ}$) and the corresponding pole is at 195.0$^{\circ}$E, 51.6$^{\circ}$N (n=6, K=20.8, $A_{95}$=12.4$^{\circ}$). Although the pole position is close to those of Jurassic period of the Kyeonggi massif and Early Cretaceous of the Kyeongsang basin. it is interpreted that the Seosan Group was remagnetized by the influence of the emplacement of the Jurassic Daebo Granite after or at the closing stage of the orogenic episode rather than under the direct effect of deformation and/or metamorphism caused by the collision.
Objective of this research was to draw the basic criteria of the compost maturity evaluation, by assessing the stability of chemical and physical properties of the bark and piggery manure byproduct composts during the composting. Colors of the mature composts were black and dark brown for the bark and piggery manure by-product composts, respectively. Good earthy odor was detected for both by-product composts after approximately 40 days composting, by which odors of the original raw materials were disappeared. pH and EC of the mature bark: compost were stabilized at 6.5 and 1dS/m, respectively. The respective values for the piggery compost were stabilized at 7.2 and 6dS/m. Organic matter contents were decreased with time to be stabilized at about 60% at the end of composting. During composting, total N contents of the bark and piggery composts were maintained at $1.1{\sim}1.5%$, and $1.5{\sim}2.2%$, respectively. For both fertilizers, $NH_4-N$ contents were increased at the initial stage bur. decreased after the middle stages of decomposition, resulting in the increase of $NO_3-N$ contents. Total inorganic N contents were increased with time. C/N ratios of both mature composts were stabilized at $25{\sim}27$. CEC of the bark compost was increased logarithmically with time and that of mature compost was 87cmol(+)/㎏. CEC of the piggery manure compost was hyperbolic function with rime and reached at 70cmol(+)/㎏ at the mature stage. Crude fiber analysis indicated that relative contents of lignin were increased with composting by compensating for the decreases of cellulose and hemicellulose contents.
Journal of The Korean Society of Grassland and Forage Science
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v.34
no.4
/
pp.288-295
/
2014
This study was conducted to evaluate the effect of mixing time for TMR (total mixed rations) mixed with corn silage on particle size, peNDF (physically effective neutral detergent fiber), laceration, and ruminal in situ dry matter degradation. The study also aimed to evaluate the effect of consumption of the TMR on the milk yield and milk components of mid-lactational dairy cows. TMRs were mixed for 30 minutes (T1) and 50 minutes (T2) using the same material. All samples were then analyzed with a Penn State Particle Size Separator (PSPS). The particle size of T1 was significantly lower in the bottom pan (8 mm>) than that of T2 (p<0.01). $peNDF_{>8.0}$ was significantly higher in T1 (17.18%) than in T2 (13.85%) (p<0.01). For ruminal in situ dry matter degradation of particle retention (>19 mm), no significant difference was found after 72 hours incubation, although T1 degradation was significantly higher after 24 hours incubation (p<0.01). Milk yield was no different between the groups of cows, whereas the milk fat from T1 fed cows was significantly higher (p<0.01). The results show that feeding TMR mixed for 30 minutes to dairy cows may improve the physical value of forage without negative effects on the milk yield and milk components.
During in vitro culture of mammalian oocytes and embryos, the cells are exposed to the risks that cause cell injury or death. Numerous studies have been reported that the cell injury may be induced by the action of free radicals generated by auto-oxidation. This study was undertaken to investigate the optimal culture condition system for in vitro culture of porcine embryos. We first evaluated the effect of culture media on the porcine embryo development. NCSU-23 and PZM-5, culture medium tested, were failed to produce significant difference on the rate of blastocyst formation. In NCSU-23, the developmental rate was slightly higher than that in PZM-5. During in vitro maturation (IVM), fertilizaton (IVF), and culture (IVC) under 5 or 20% oxygen ($O_2$), the rates of cleavage and development were insignificantly different from each other under our culture condition (20% $O_2$, in NCSU-23), the mean cell number per blastocyst was $40{\pm}10$. These results showed that medium and $O_2$ concentration had no significant effect on the development of porcine embryos.
F. moniliforme MRC 826, a common fungal contaminant of com, has been known to produce a group of mycotoxins, the fumonisins. By thin layer chromatography, fumonisin $B_{1}$ was detected in the F. moniliforme MRC 826 com culture material(CM) extracts. This study was performed to compare the toxicity and carcinogenicity of F. moniliforme MRC 826 CM with those of aflatoxin $B_1(AFB_1)$ in rats. The toxicity was tested over a period of 7 days in ten female Sprague-Dawley (SD) rats. Treatment group were fed a 1 : 1 mixture(wt/wt) of ground CM and basal diet in powder form, while other negative control group were given basal diet alone. The principal pathological changes in rats treated with 50% CM were hepatocellular hydropic degeneration and renal tubular necrosis. The cancer-promoting activity of CM was evaluated in the rat liver diethylnitrosamine-two thirds partial hepatectomy(DEN-PH) model for carcinogenesis. 70 male SO rats(ca. 170 g) were randomized into 5 groups. Group I served as the positive controls and received the basal diet containing 2 ppm $AFB_{1}$ group 2 received 5% CM, group 3 received 2.5% CM, group 4 received 5% normal com and group 5 received 2.5% normal com. 5% treated group showed cancer promoting activity in rat liver using DEN as initiator and the induction of glutathione S-transferase placental form positive foci as an end point after 6 weeks of promotion.
Ji, Jong-Hu;Park, Sang-Eun;Kim, Young-Yul;Kim, Weon-Yoo;Kewon, Oh-Su;Jang, Dong-Gyun;Moon, Chang-Yun
Clinics in Shoulder and Elbow
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v.11
no.2
/
pp.104-111
/
2008
Purpose: The aim of this study is to analyze the clinical results of using the technique of rotator cuff repair without parting the biceps long head from the glenoid for large or massive tear of the rotator cuff. Material and Methods: Form January 2005 to January 2007, we performed the arthroscopic biceps repair with incorporating suture to the rotator cuff for 21 patients with large or massive rotator cuff tear. The mean follow up period was 23 months (range: 6-48months). The number of males and females was 9 and 13, respectively. The age distribution ranged from 47 to 73 years with a mean age of 60.3 years. We compared the preoperative score with the postoperative scores using the University of California Los Angeles (UCLA) score, the shoulder index of the American Shoulder and Elbow Surgeons (ASES) and a simple shoulder test (SST). Results: The improvement in the VAS, ASES and the UCLA and SST scores was statistically significant at the final follow up (average follow-up 23 months) (p>0.05). Two of nine cases were found to have partial tear with continuity but seven cases were found to have complete tear according to the ultrasonography and MRI. Conclusion: The technique of rotator cuff repair without parting the biceps long head from the glenoid for large or massive tear of the rotator cuff is considered to be recommendable.
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