• FLOWER RESEARCH JOURNAL
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• v.18 no.1
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• pp.33-37
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• 2010

Effects of cutting time of double flowered Hydragea serrata for. acuminata native to Korea on flowering and growth of following year were investigated in order to develop as a pot plant. Cuttings were carried out every 10th day from April to September in 2008. The cuttings were directly placed in 15 cm diameter pots. Longest plant was obtained from May cuttings and their average heights were 31.7 cm, and followed by April and July cuttings. Plant heights of August and September cuttings were 23.6 cm and 22.0 cm, respectively. In contrast, growth and height of June cutting were abnormally small. Leaf length, leaf width and petiole length of August and September cutting were reduced, but numbers of leaves were not changed. Average flower cluster numbers of May cuttings were 4 flower clusters, and July, August and September cuttings were 3 clusters while only 1 flower cluster per a plant was obtained during June. Biggest diameter of flower crown was observed from May cuttings and the size was 10.3 cm diameter; however, smaller flower crown size was observed after July cuttings. The best overall flower appearance was observed from May cuttings, and the worst was June cuttings. Even though plant height of August and September cuttings were reduced, flowering aspect and ornamental value were normal, but blooming times were retarded according to late cutting time.

• Korean Journal of Medicinal Crop Science
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• v.7 no.3
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• pp.200-204
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• 1999

Ligusticum chuanxiong has been an important oriental crude drug and cultivated for a long time in Korea. Ligusticum chuanxiong has been vegetatively propagating crop without seed due to its sterility and would be an allodiploid derived from the hybridization of two allied species. Generally, vegetative propagation is more costly per unit propagule than sexual(seedling) propagation. This study was conducted to find out the techniques of mass propagation by stem cutting for reduced seedling cost. When cutted on August 30 with 1st to 2nd internode, its highest rooting percentage was 81 percent. Rhizome yield of plants cutted in 50 percent on September 1 decreased to 9 percent less than control (rhizome propagation). Rooting percentage of rooted cutting plants after field planting on October 25 was the highest being 87 percent. Rhizome yield with planting appeared 127 and 120kg/10a on September 25 and October 25, respectively.

• Journal of Plant Biotechnology
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• v.42 no.3
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• pp.228-234
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• 2015

Using either the apical or axillary bud of the endangered species Abeliophyllum distichum Nakai, we tested the effect of bud position and culture method on shoot proliferation and rooting. In shoot proliferation, the axillary bud explant was more effective than the apical bud and the effect was fostered by BA treatment, whereas no differences were observed in shoot elongation by the explant position. Spontaneous rooting was observed in the MS basal medium and resulted in conspicuous differences in the explant position : more than 80% in apical bud explant and 28% in axillary bud explant was achieved, respectively. The positional effects were also observed in BA pre-treatments: generally vertical culture method appeared to be better in shoot proliferation, growth, and rooting than that of the horizontal culture method regardless of the BA pre-treatment duration. The highest shoot multiplication was achieved through the vertical culture method with axillary bud explant, whereas the best shoot elongation and rooting was obtained using the vertical culture method with the apical bud explant. Apical bud explant was superior to axillary bud explant in ex vitro micro-cuttings and revealed a significant difference in shoot growth and root development. The above results suggest that explant position and culture method influence the efficiency of micropropagation for a rare and endangered plant Abeliophyllum distichum.

• Korean Journal of Environment and Ecology
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• v.15 no.1
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• pp.57-68
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• 2001

해안지역의 녹화 및 조경용으로 유용한 내염성 식물자원임과 동시에 서식지가 급격히 감소하고 있는 순기비나무를 대상으로 하여 생장특성과 번식방법을 규명하기 위한 연구를 수행하였던 바, 그 결과를 요약하면 다음과 같다. 1. 줄기의 생장형태는 포복줄기와 직립줄기가동시에 생장하고, 5개 주요 군락지에 직립줄기의 길이는 평균 46.6cm 이고, 포복경의 초대 길이는 7.6cm에 달하였다. 2. 종작발아율은 황적색의 무처리 종자와 $GA_3$처리 종자에서 71.0~72.3%로 가장 높았으나, 두 처리군사이에는 발아율의 차이가 없었다. 종피현화를 목적으로 한 황산처리는 오히려 종자의 발아를 억제하였다. 3. 종피 색깔이 황적생인 종자는 흑갈색 종자에 비해 평균 21.1%(13~30%)발아율이 높았다. 실득모율은 30% 미만으로 아주 낮았으나 흑갈색 종자에 비해 황적색 종가자 약 2배 높았다. 또한 $LD_{50}$은 황적색 종자가 흑갈색 종자보다 10~20일 짧았다. 4. 종피 색깔과 화학처리가 같은 조건에서 기건저장한 종자와 노천매장한 종자사이에는 발아율, 식득묘율, $LD_{50}$등의 차이가 없었다. 5. 기건저장한 황적색 종자가 바다모래에서 30.3%의 발아율과 10.1%의 실득모율을 나타냈으나, 갯벌흙에서는 전혀 발아되지 않았다. 6. 삽목번식의 경우 추삽에서 발근육은 녹지삽이 가장 높았고, 다음이 반지숙삽, 숙지삽의 순이었다. 발근율이 가장 높은 것은 NAA 200ppm+sucrose 2%를 처리한 녹지로서 96.7%의 발근율을 나타내었다. 발근수는 전반적으로발근율에 비례하는 경향이었다. 7. 하삽에서 NAA 200ppm+sucrose 2%를 처리한 녹지, 반숙지, 숙지의 토양별 발근율은 vermiculite+ perlite(1:1)와 마사토에서는 81.1~87.8%로 높았고, 모래와 해상에서 48.9~77.8%로 낮았다.

• Journal of agriculture & life science
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• v.44 no.3
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• pp.23-30
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• 2010

The optimal growth condition of in vitro stem cutting of Dendrobium nobile 'Hamana Lake Dream' ${\times}$ 'No. 55' was investigated. Among various media and their concentrations, MS media had better effect on the growth of micro stem cutting than Hyponex media in all concentration levels except stem length. The activated charcoal concentration in MS media showed different effects on number of stem and root, leaf length, and fresh weight: the most effective in the range of 0.1 to 1.0 g/L and barely effective above 2.0 g/L. Addition of agar 5 g/L, sucrose at 40 g/L, and peptone at 1 g/L to MS media increased significantly stem length, leaf width, and fresh weight, internode length and number of roots, and the number of stem and leaves. On the other hand, addition of gelite with any concentration had no effect on the growth of micro stem cutting compare to that of control. The optimal temperature for growth of micro stem cutting was $28^{\circ}C$. Under the same temperature, MS medium was better than Hyponex medium for the growth of stem. In addition, sucrose at 40 g/L was the most effective on growth at $28^{\circ}C$.

• Journal of Korean Society of Forest Science
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• v.82 no.3
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• pp.221-226
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• 1993

Methods for shoot proliferation via pulse treatment onto the microshoots of Quercus acutissima, and ex vitro root induction using peat plug systems of the microshoots of 4 oak trees were described. Pulsing solution was prepared by the addition of BA and/or BA plus zeatin onto the aqueous WPM and sterilized distilled water. Using the solution, pulsing time was adjusted at different levels(0. 1, 2, 5. 9, and 24 hours). Although the effect of pulsing solution prepared by the addition of cytokinins onto the sterilized distilled water was slightly lower in shoot proliferation rate, a little higher in shoot elongation was observed compared with that of aqueous WPM. One hour of pulse treatment revealed best in shoot proliferation and its elongation, whereas the increment of pulsing time slightly suppressed the response. In addition, prolonged pulse time resulted high frequency of hyperhydric shoot appearance. Single treatment of BA was better in shoot proliferation than that of BA combination with zeatin, whereas the latter treatment usually showed rapid and healthy shoot growth. For ex vitro root induction using peat plug systems, black oaks(Q. acutissima and Q. variabilis) revealed excellent rootability compared with white oaks(Q. serrata and Q. mongolica). Shoot-tip necrosis of white oaks eras one of the big problems for survival. In this study, we discribed the effect of pulse treatment, successful ex vitro rooting system by the incorporation of peat plug, and the possibilities for the overcoming the obstacles on micropropagation of oaks.

• Korean Journal of Plant Tissue Culture
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• v.22 no.6
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• pp.335-337
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• 1995

The effect of plant growth regulators on proliferation of shoot from stem node culture of Japanese yew (Taxus cuspidata Sieb. et Zucc.) was studied using Quoirin and Lepoivre (1977) medium. Among the cytokinin tested, BAP, kinetin, and thidiazuron at various concentrations had no effect on shoot multiplication However when zeatin at 5$\times$10$^{-5}$ M was added to the medium, an average of 6 shoots were regenerated per explant after 8 weeks of culture. The ratio of rooting ex vitro was remarkably increased up to 34% by dipping the basal end in 0.5 to 1.0% IBA on talc compared with 3% in vitro rooting. Rooted plantlets were acclimated in greenhouse conditions for one month and successfully transplanted to the field.

• Horticultural Science & Technology
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• v.30 no.6
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• pp.686-693
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• 2012

To develop a technique for mass-propagation of virus-free sweet potato [Ipomoea batatas (L.) Lam.] plant using nutrient film technique (NFT), the growth characteristics of 4 cultivars as affected by nutrient solution composition and cutting size were investigated. 72 cells (35 mL/cell) plug trays filled with vermiculite and perlite (1:1, v/v) were used. Vine length, fresh and dry weights of virus-free plants were the greatest in the nutrient solution recommended by National Horticultural Research Station in Japan, followed by that recommended by National Institute of Horticultural & Herbal Science in Korea, and Yamazaki's nutrient solution for lettuce. The growth of uppershoot cuttings was the best among 4 subsections of cutting. Vine length, and fresh and dry weights increased in the longer cutting treatments, and were better in 'Shinzami' and 'Yeonhwangmi' than those in 'Mannami' and 'Shincheonmi'. Vine diameter and length of the longest root were not significantly affected by the cutting size and cutting source. The growth characteristics of the single node cutting were not significantly different from those in 2-node cutting. The efficiency of rapid mass-propagation could be promoted with single node cuttings and uppershoot cuttings grown in NFT system.

• Journal of Bio-Environment Control
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• v.17 no.1
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• pp.60-65
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• 2008

For autumn field cutting of Sedum sarmentosum, the effects of cutting site (distal, middle, and proximal) and planting method (space drill seeding, drill seeding, and broadcast seeding) on survival rate and growth characteristics were investigated at pre- and post-winter season. Plant height root length, stem number per plant, number of branch per plant, fresh weight of shoot, and dry weight of shoot were significantly superior in distal site at pre-wintering (40 days after cutting). At post-wintering (May 10th), stem number per plant, fresh weight and dry weight per $m^2$ in cutting of distal site showed a significant increasement compared to the cutting of proximal site. At pre-wintering (40 days after cutting), the growth in space drill seeding was well than that in drill seeding and broadcast seeding, and fresh weight and dry weight per $m^2$ in space drill seeding were high in order of space drill seeding, broadcast seeding, and drill seeding. At post-wintering (May 10th), stem number per plant, fresh weight and dry weight per $m^2$ in space drill seeding were significantly increased than those in drill seeding. Accordingly, the cutting using distal site of stem in autumn field cutting was desirable for the growth and shoot yield. The space drill seeding showed the highest yield potential among three seeding methods, but broadcast seeding was favorable in saving of labor, because the fresh weight of shoot in broadcast seeding was similar in the space drill seeding at post-wintering.

• Korean Journal of Environment and Ecology
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• v.21 no.6
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• pp.544-553
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• 2007

This study is focused on the cultivation of seedlings and cuttings of roundleaf chastetree(Vitex rotundifolia L. f.) according to the sorts of soil and fertilization methods. The results are summarized as follows: When roundleaf chastetree seedlings(aged 1-0) and cuttings(aged 1-0) were cultivated in three different kinds of soil- sand, granite soil, and yellow silt- without any fertilization, 70% of the seedlings survived in sand and granite soil, and 35% did in yellow silt; 90% of the cuttings survived in granite soil and 80% did in sand and yellow silt. The general growth of both seedlings and cuttings was good in granite soil, and sand but relatively inferior in yellow silt. Seedlings showed good stem growth while cuttings had fine root growth. In case of cultivation of seedlings with different kinds and quantities of fertilizer adjusted and with granite soil as basic soil, seedlings and cuttings survived in the experimental section where only the organic fertilizer was used as basic fertilization; in contrast, seedlings grown in the experimental section were all dead where both inorganic and liquefied fertilizer were used. Cuttings showed considerably higher survival rates than seedlings at the experimental fertilization section; particularly, the survival rate of cuttings was twice as much as that of seedlings at the experimental section with low fertilization. This study results revealed that cuttings are desirable than seedlings in general rootage, growth, adaptability to soil, and cuttings have lower demand for soil fertility in time of growing roundleaf chastetree saplings.