• Korean Journal of Medicinal Crop Science
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• v.15 no.3
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• pp.199-202
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• 2007

This study was carried out to provide pest control information for the cultivation of Eleutherococcus senticosus Max. As a result of investigation of the diseases and insect pests, three pathogens and three insect pests were identified from the field sample, respectively. The identified insects pests were aphids, stinkbugs and Bothrogonia japonica. Occurrenre peaks of aphids were occurred on June and August, and generally young leaves and flower buds were injured mainly. The identified diseases were black ring spot caused by Phoma sp., gray mold caused by Botrytis cinerea and leaf blights caused by Rhizoctonia solani. The black ring spot was the most severe disease and was investigated from the beginning of June, and the incidence of the disease was 30% higher than that on September. As a result of growth test under different temperature conditions on PDA, these pathogens showed the best mycelial growth rate between 25 $^{\circ}$C and 30 $^{\circ}$C, and these results indicate that these diseases occur on hat summer season.

• The Korean Journal of Pesticide Science
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• v.19 no.2
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• pp.125-133
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• 2015

Entomopathogenic fungi have been studied to develop for biological control agents as an alternative to chemical control agents in insect pest management. Two Lepidopteran insects, Spodoptera exigua and Plutella xylostella, are serious insect pests infested various crops, but not effectively controlled by commercial chemical pesticides due to its high insecticide resistance. A fungal isolate was isolated from S. exigua larvae collected from green onion field in Andong, Korea. To identify the fungal isolate, 18srRNA sequence for internal transcribed spacer (ITS) and ${\beta}$-tubulin regions were sequenced. The ITS and ${\beta}$-tubulin sequence were highly matched to Beauveria bassiana and morphological characteristics also was fit to known B. bassiana. Finally, isolated fungus has identified as B. bassiana and named B. bassiana ANU1. The result of bioassay, median lethal concentrations were $2.7{\times}10^3$ and $0.9{\times}10^3conidia/ml$ and medial lethal times were 65.6 and 60.8 h to S. exigua and P. xylostella, respectively. B. bassiana ANU1 showed high pathogenicity to two insect pests from $20^{\circ}C$ to $30^{\circ}C$ at 50% relative humidity (RH) and more than 40% RH at $25^{\circ}C$ with $10^7conidia/ml$ of concentration.

• Korean journal of applied entomology
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• v.47 no.1
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• pp.37-44
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• 2008

Oriental fruit moth, Grapholita molesta, is a serious pest on apples. To control this pest in an environmentally friendly method, mating disruption strategy using sex pheromone has been developed. Area-wide application of mating disruption has been needed to be effective, with little understanding on how much size of apple cultivating area should be treated in one time application of the mating disruption technique. On this matter, we needed to determine a minimal mating active zone of G. molesta that should be applied with mating disrupters to be effective. Molecular markers to discriminate a specific population should be developed to trace population migration for reproductive behaviors. Here we developed two effective molecular markers using random amplified polymorphic DNA (RAPD) technique. Different field populations of G. molesta, based on locations and seasons, were analyzed with these markers. In a specific location, G. molesta populations varied in genetic composition with different seasons. Different local populations showed differential variation according to their relative distances among apple orchards. In overall, genetic variation among different populations became lessen with progression of seasons.

• Korean journal of applied entomology
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• v.57 no.3
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• pp.151-160
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• 2018

Anoplophora chinensis and A. glabripennis is classified as important quarantine Cerambycidae beetles worldwide. These beetles cause serious damage on natural host plants and export bonsai trees in Korea. We evaluated efficacy of phosphine fumigant for control of two Cerambycidae beetles in bonsai. In the preliminary test to achieve CT (Concentration x time) values of phosphine to Cerambyicdae beetles larvae, we used Monochamus alternatus of larvae as an alternative insect. A 24 h phosphine fumigation with $2g/m^3$ phophine achieved 100% eggs mortality against Anoplophora chinensis and A. glabripennis. Larva of A. chinensis was also completely controlled when treated for 168-h at same dose desiccator. 100% mortality on larval stage of two beetle species were achieved both in dormancy (January) and vegetative period (February) of bonsai when treated with $2g/m^3$ phosphine. However, phosphine fumigation against vegetative period of Acer palmatum, Carpinus turczaninowii and Malus sieboldii bonsai showed slight phytotoxicity (shorten leaf and flower size and delayed flowering).

• Korean journal of applied entomology
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• v.59 no.1
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• pp.55-64
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• 2020

Smart greenhouses are primarily used for growing fruits and vegetables, such as sweet peppers, tomatoes, strawberries. Although the number of smart greenhouses has been increasing exponentially, no studies have been performed to evaluate the state of smart greenhouses in Korea. Therefore, this study was conducted to determine current state of smart greenhouses with regard to greenhouse specifications, crop growing methods, pests, and user satisfaction in Korea. Contact information for smart greenhouses was provided by the officials of local agricultural research and extension services. This survey was conducted by visiting each greenhouse. Results showed that approximately 50% of surveyed smart greenhouses were between 3,300 ㎡ and 6,600 ㎡. The most frequently chosen method for pest control was chemical pesticides (97.1%). Powdery mildew and gray mold comprised 54.4% and 33.8% of the crop diseases, respectively. All tomato greenhouse farmers considered whiteflies the most problematic pest. In contrast, 76.5% and 70.6% of sweet pepper farmers believed thrips and aphids posed significant threats, respectively. The mean satisfaction score was 7.5 out of 10 points, with 10 being "extremely satisfied". These results will aid in decision making with respect to the management of current smart greenhouses and the design of future smart farms in Korea.

• Journal of Applied Biological Chemistry
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• v.60 no.3
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• pp.271-277
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• 2017

Ethyl formate has been used for the control of insect pests by fumigation. However, there were not many reports to show its target site of fumigant toxicity on insect pests since its first use in the agricultural industry. In the present study, we showed the presumable target sites of ethyl formate fumigation in insect pests using Myzus persicae nymphs. After ethyl formate fumigation, the nymphs of this species were collected and the changes at the biochemical and molecular level were determined. The activity of cytochrome c oxidase (COX) was approximately two-fold higher after ethyl formate fumigation. In addition, the expression levels of acetylcholinesterase (AChE) decreased gradually with increasing ethyl formate concentration. These two findings suggested that COX and AChE might be the major target sites of ethyl formate fumigation. In addition to these results, the analysis of lipid content using MALDI-TOF MS/MS identified 9 phospholipids differently generated 2-fold higher in the ethyl formate-treated nymphs than that in the control nymphs, thereby leading to changes in cell membrane composition in M. persicae nymphs. Therefore, the ethyl formate fumigation caused lethal effects on M. persicae nymphs by changing COX activity, AChE gene expression, and phospholipid production.

• Journal of Life Science
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• v.17 no.6 s.86
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• pp.828-830
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• 2007

To determine the effects of newly developed insecticide (Ultra) on Sycamore lace bug (Corythucha ciliata), ten leaf discs were sprayed with the Ultra solution including the other selected commercialized insecticide solutions such as Carbaryl and Pirimicarb, and also with a water-sprayed control. According to the results, the control effect of Ultra on the adults and immatures was respectively 98.9% and 97.5% after 48hr, and the corresponding values were higher than that of the other tested insecticides (i.e Carbaryl showed 84.6% for adults and 85.6% for immatures; Pirimicarb showed 83.0% for adults and 90.7% for immatures). For the Susceptibility test, LC$_{50}$ value of Ultra showed the lowest as 1.5ppm, the value of Carbaryl and Pirmicarb were 3.7 ppm and 30.4 ppm, respectively. Therefore, the insecticidal effects were better for the newly developed insecticide than the commercialized insecticides.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.22 no.1
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• pp.231-236
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• 2021

The Aphid, Myzus persicae, and the bean bug, Riptortus clavatus, are major insects in crops. This study examined the insecticide susceptibility and phytotoxicity of insecticides dispersed using an Unmanned Aerial Vehicle (UAV, multi-copter) against the insects. Sulfoxaflor suspension concentrate (SC, 16X) on potato fields and etofenprox, methoxyfenzide suspo-emulsion(SE, 8X) on soybean fields were dispersed after deploying water-sensitive paper within the field to measure the distribution pattern and coverage index of the falling insecticide. Both insecticides showed a controlled mortality of 76.4% against aphids and 97.5% and 94.4% against the 2nd nymphal, and 5th nymphal stage of the bugs, respectively. The droplet distribution was less than 0.5mm, and coverage analysis revealed an inside and outside coverage of 3.1 and 1.6, respectively. The surrounding area was affected by insecticide spraying using a multi-copter. This study is expected to help expand UAV control and use it safely in the future.

• Korean journal of applied entomology
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• v.61 no.4
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• pp.529-537
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• 2022

The susceptibilities of five insecticides to the larvae of Spoladea recurvalis, a major pest of spinach, were evaluated. Lufenuron EC, chromafenozide EC, chlorantraniliprole WP, tebufenozide WP, and pyridalyl EW showed 90% or more insecticidal activity at dilution concentrations of 2 (12.5 ppm), 4 (12.5 ppm), 8 (2.5 ppm), 4 (20.0 ppm), and 8 (12.5 ppm), respectively. At their recommended concentrations, Chromafenozide EC, chlorantraniliprole WP, tebufenozide WP, and pyridalyl EW showed very high insecticidal activity of 98.3%, 100%, 95.0%, and 100%, respectively, after 72 h on S. recurvalis larvae exposed after 7 days of treatment. Field tests at two spinach greenhouses in Hwaseong and Yeoncheon showed a high control effect of over 90% after 7 days of treatment in both areas. In addition, the insecticides can be used as exclusive agents for S. recurvalis that occurs in spinach, as there is no phytotoxicity even at double doses.

• Korean journal of applied entomology
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• v.62 no.3
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• pp.117-124
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• 2023

The management of Ricania spp. is difficult because this pest has a wide host range and diverse habitats such as agricultural, suburban, urban, and forested areas. However, the researches for Ricania spp. management have been focused on only agricultural crops. Thus, it is required to determine the arboreal host preference of Ricania spp. at the surrounding areas of the farms to increase its management efficiency. To determine its host preference at arboreal plants, we reviewed the previous studies and investigated the densities of Ricania spp. at woody plants with high ecological importance but insufficiently studied. This study identified 120 species in 53 families of arboreal hosts of Ricania spp. Only Cornus officinalis and Styrax japonicus were preferred by all developmental stages of Ricania spp. The host preference of Ricania spp. was changed according to its developmental stages. This phenomenon would be caused by that each developmental stage of Ricania spp. would prefer different parts of woody plant, and require different nutrients for its survivor and reproduction. These results of this study could be helpful to make a plan of comprehensive management strategies for Ricania spp.