• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.1
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• pp.537-545
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• 2018

In this study, transcriptome analysis was conducted to collect various information from Fagopyrum esculentum and Fagopyrum tataricum during the early germination stage. Total RNA was extracted from the seeds and at 12, 24, and 36 hrs after germination of Jeju native Fagopyrum esculentum and Fagopyrum tataricum and sequenced using the Illumina Hiseq 2000 platform. Raw data analysis was conducted using the Dynamic Trim and Lengths ORT programs in the SolexaQA package, and assembly and annotation were performed. Based on RNA-seq raw data, we obtained 16.5 Gb and 16.2 Gb of transcriptome data corresponding to about 84.2% and 81.5% of raw data, respectively. De novo assembly and annotation revealed 43,494 representative transcripts corresponding to 47.5Mb. Among them, 23,165 sequences were shown to have similar sequences with annotation DB. Moreover, Gene Ontology (GO) analysis of buckwheat representative transcripts confirmed that the gene is involved in metabolic processes (49.49%) of biological processes, as well as cell function (46.12%) in metabolic process, and catalytic activity (80.43%) in molecular function In the case of gibberellin receptor GID1C, which is related to germination of seeds, the expression levels increased with time after germination in both F. esculentum and F. tataricum. The expression levels of gibberellin 20-oxidase 1 were increased within 12 hrs of gemination in F. esculentum but continuously until 36 hrs in F. tataricum. This buckwheat transcriptome profile analysis of the early germination stage will help to identify the mechanism causing functional and morphological differences between species.

• Korean Journal of Environmental Biology
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• v.39 no.4
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• pp.581-589
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• 2021

Using visible-near infrared(Vis-NIR) spectra combined with machine learning methods, the feasibility of quick and non-destructive classification of Convolvulaceae species was studied. The main aim of this study is to classify six Convolvulaceae species in the field in different geographical regions of South Korea using a handheld spectrometer. Spectra were taken at 1.5 nm intervals from the adaxial side of the leaves in the Vis-NIR spectral region between 400 and 1,075 nm. The obtained spectra were preprocessed with three different preprocessing methods to find the best preprocessing approach with the highest classification accuracy. Preprocessed spectra of the six Convolvulaceae sp. were provided as input for the machine learning analysis. After cross-validation, the classification accuracy of various combinations of preprocessing and modeling ranged between 43.4% and 98.6%. The combination of Savitzky-Golay and Support vector machine methods showed the highest classification accuracy of 98.6% for the discrimination of Convolvulaceae sp. The growth stage of the plants, different measuring locations, and the scanning position of leaves on the plant were some of the crucial factors that affected the outcomes in this investigation. We conclude that Vis-NIR spectroscopy, coupled with suitable preprocessing and machine learning approaches, can be used in the field to effectively discriminate Convolvulaceae sp. for effective weed monitoring and management.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.60 no.3
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• pp.293-299
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• 2015

High temperature is one of major environmental stress. Heat tolerance managing is difficult through the phenotypic selection, so marker assistant selection (MAS) using molecular markers like as RAPD, SSR etc. was tried to select useful traits for heat tolerance. Fourteen SSR markers reported by previous research were selected for this research. We tried to evaluate 14 SSR markers for MAS using 31 useful wheat resources including 24 crossing line from Turkey, six Korean wheat cultivars and Chinese spring. The average of the number of alleles and PIC values in this study were 6.14 and 0.64, respectively. Two major clades and four sub clades were grouped by phylogenetic tree using UPGMA. Four Korean wheat cultivars were distinct from other Turkey resources in the phylogenetic dendrogram. From the results, we expected that these markers were able to adapt to screening wheat genotyping for heat tolerance.

• Proceedings of the Korea Society for Industrial Systems Conference
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• 1999.12a
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• pp.121-129
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• 1999

When continuous media are transmitted over the communication networks, asynchrony which can not maintain temporal relationships among packets may occur due to a random transit delay. There exist two types of synchronization schemes ; for guaranteed or non-guaranteed resource networks. The former which applies a resource reservation technique maintains delay characteristics, however, the latter supply a best-effort service. In this paper, I propose a intra-media synchronization scheme to transmit continuous media on general networks not guaranteeing a bounded delay tome. The scheme controls transmission times of the packets by estimating next delay time with the delay distribution. So, the arriving packets may be maintained within a limited delay boundary, and playout will be performed after buffering to smoothen small delay variations. The continually increasing delay due to network overload causes buffer underflow at the receiver. To solve it, the transmitter is required to speed up instantaneously. Too much increase of transmission-rate may cause network congestion. At that time, the transmitter drops the current packet when informed excessive delay from the receiver.

• Weed & Turfgrass Science
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• v.3 no.1
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• pp.29-40
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• 2014

Research was initiated to investigate early establishment characteristics and germination pattern of Kentucky bluegrass (KB, Poa pratensis L.). Fifteen cultivars were evaluated under alternative conditions (8 hours light at $25^{\circ}C$ and 16 hours dark at $15^{\circ}C$). Significant differences were observed in germination characteristics and germination pattern among KB cultivars. A final germination percentage differed in cultivars, being 75.25 to 89.50%. The first germination was initiated between 6 and 9 DAS (days after seeding). As for the first germination percentage, 'Brilliant' and 'Midnight II' produced 14.50% and 23.00%, respectively, while the others were most below 5%. Days to 75% germination were between 15.08 and 28.80 DAS. 'Excursion', 'Midnight II', 'Odyssey', 'Midnight' and 'Courtyard' were fastest. The slowest cultivar was 'Voyager II', being over 28 DAS, which were 13 to 14 days slower than the fastest ones. Considering the first germination percentage, days to the first germination, days to 75% germination, and germination pattern, 'Midnight II', 'Excursion' and 'Midnight' were regarded as excellent cultivars under alternative conditions. From this study, information on differences in germination characteristics and patterns would be practically useful for a golf course construction when established with KB.

• Korean Journal of Plant Resources
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• v.35 no.4
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• pp.464-470
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• 2022

In this study, the morphological characteristics of flower and fruit in five varieties (Hancho 915ho, 930ho, 10ho, 1020ho, 1030ho) from Zanthoxylum schinifolium were investigated as new varieties with different fruiting seasons. Qualitative and quantitative characteristics were investigated, spectively. For quantitative characteristics can be measured numerically, such as size and number, the varieties were identified using ANOVA and Scheffe's multiple range test. In addition, the validity of whether the quantitative characteristics of five varieties can be explained by factor analysis was reviewed. Using UPGMA, the relationship between the five varieties was confirmed by dendrogram. As a result of ANOVA and Scheffe's multiple range test there was significant difference in inflorescence width and female flower length. It had a high contribution to distinguishing varieties, which was similar to the PCA results using factor analysis. As a result of dendrogram using cluster analysis, Hancho 915ho, 1020ho and 1030ho were similar, but there was a distinct difference in the characteristics of different flowering and fruiting periods, confirming that they were different varieties.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.10a
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• pp.28-28
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• 2019

본 연구는 멸종위기 아고산수종 구상나무 잔존집단 복원을 위해서 묘목처리 및 입지환경이 구상나무묘목 초기생장에 미치는 영향을 구명하고자 수행하였다. 연구대상지는 경상남도 금원산으로 해발 1,300 m 이상 능선부(1입지)와 구상나무 잔존집단(2입지)을 선정하였다. 식재한 구상나무 실생묘는 지리산 5개 개체에서 종자를 채취하여 2014년도 파종한 1~5묘 1,350본을 이용하였다. 2019년 5월 연구대상지의 조릿대를 제거 후 무처리, 분해포트, 균근균 접종 실생묘를 9개 구역으로 나누어 3반복씩 입지별 0.15 ha, 4,500 본/ha 밀도로 식재하였다. 식재 후 2019년 5월부터 8월까지 입지별 기상데이터를 수집하였고 묘목의 생존율, 수고, 1년지 생장량 및 근원경을 측정하였다. 환경분석 결과 1입지(88.2%, $42.8umole{\cdot}m^{-2}{\cdot}s^{-1}$)가 2입지(84.5%, $29.4umole{\cdot}m^{-2}{\cdot}s^{-1}$)에 비해 대기습도와 광량이 높게 나타났다. 구상나무 실생묘의 생존율은 모든 입지에서 100%로 나타났다. 입지별 생장특성 분석 결과 1입지가 수고 17.3 cm, 1년지 생장 4.72 cm 및 근원경 6.20 mm로 2입지(19.0 cm, 3.88 cm, 5.31 mm)에 비해 양호한 것으로 나타났다. 묘목 처리에 따른 분석 결과 무처리와 분해포트 묘목이 모든 입지에서 초기 생장이 양호하였고 균근균 접종 처리는 초기에는 비교적 낮은 생장특성이 나타났다. 결과적으로 대기습도와 광량이 높은 환경 조건이 구상나무 묘목의 초기 생장에 긍정적인 영향을 주었으며 묘목 처리에 따른 생장특성은 지속적인 모니터링이 필요한 것으로 나타났다.

• Advanced Industrial SCIence
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• v.2 no.4
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• pp.36-43
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• 2023

Native plants thrive naturally in specific areas without human intervention, offering significant potential as genetic resources and biotechnological assets across multiple sectors. To harness this potential, our focus was on analyzing domestic patents related to native plants, investigating their uses, effectiveness, active components, and extraction methods. Using the Korea Forest Service's National Standard Plant List, we gathered data from 988 patents on native plants and 430 patents on the use of native plant seeds. This comprehensive patent analysis aimed to reveal research patterns, technology levels, and emerging trends. The goal is to identify research trends, current technology levels, and provide insights for future patent applications involving native plants.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
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• v.15 no.2
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• pp.127-135
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• 2020

The purpose of this paper is to classify individual startups by growth stage based on data-based quantitative criteria. This is to provide a basis for systematic support for government startups based on accurate statistics on the startup growth process. This startups were the TIPS (Tech Incubator Program for Startup) support company, which used a relatively reliable startup. We found seed money to complete MVP (Minimum Viable Product) within 1.5 years after establishment, verified PMF (Product-Market Fit) within 1 year, attracted Series A investment within 2.5 years after establishment, and successfully commercialized it. It attracted Series B investment for stable growth within 1.5 years (Series B investment within 4 years from start-up). The results of the study, the division of government programs that support stage-based startup commercialization, that is, within three years and within seven years of establishment, is significant to date. Three directions are suggested for future research. First, develop indicators for monitoring startup growth stages. Second, it continuously updates the annual changes and tracks the growth stages of individual startups. Third, we discover the successful growth law of technology-based startups by applying in-depth case analysis of successful startups to the model.

• Journal of Plant Biotechnology
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• v.39 no.3
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• pp.121-126
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• 2012

To determine whether FT-IR spectroscopy combined with multivariate analysis for whole cell extracts can be used to discriminate major leguminous plant at metabolic level, seed extracts of six leguminous plants were subjected to Fourier transform infrared spectroscopy (FT-IR). FT-IR spectral data from seed extracts were analyzed by principal component analysis (PCA), partial least square discriminant analysis (PLS-DA) and hierarchical clustering analysis (HCA). The PCA could not fully discriminate six leguminous plants, however PLS-DA could successfully discriminate six leguminous plants. The hierarchical dendrogram based on PLS-DA separated the six leguminous plants into four branches. The first branch was consisted of all three Vigna species including Vigna radiata var. radiate, Vigna angularis var. angularis and Vigna unguiculata subsp. Unguiculata. Whereas Pisum sativum var. sativum, Glycine max L and Phaseolus vulgaris var. vulgaris were clustered into a separate branch respectively. The overall results showed that metabolic discrimination system were in accordance with known phylogenic taxonomy. Thus we suggested that the hierarchical dendrogram based on PLS-DA of FT-IR spectral data from seed extracts represented the most probable chemotaxonomical relationship between six leguminous plants.