• Journal of Life Science
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• v.16 no.2 s.75
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• pp.352-356
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• 2006

Quantity of protein, amino acid, and sugar leaked from seeds was greater as the viability of seeds was dropped by the time elapsed of seed aging treatment. In the seeds with the artificial aging treatment for 20 days, 35.8 mg of protein was leaked on the 4th day after soaking, which was 6.9 times higher than that of control. Leakage of amino acid was also higher from low quality seeds treated with the aging treatment. In the seeds with the aging treatment for 20 days, $36.5{\mu}g$ fig of sugar was leaked on the 4th day after soaking, which was 2.8 times higher than that of control. The leakage of inorganic compound was higher from the low quality seeds, and leakage of total protein, amino acid, and sugar. According to .the quantity of leakage, water soluble compounds, which can be used for the assessment of seed quality without any destruction, were protein and potassium. Germination rate and percentage of seeds were dropped with the seed aging treatment, and the seed viability could be recovered by priming treatment. This phenomenon was very clear when the low quality seeds were germinated at low temperature.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.4
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• pp.407-412
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• 1995

Experiments were conducted to obtain information on separation of nonviable seeds from seed lots by the nondestructive ways. Seeds of sesame, welsh onion and lettuce were artificially aged at 90% relative humidity and 45$^{\circ}C$ to get different seed qualities. The relationships between seed quality and leakage of total sugars, amino acids, and proteins into soaking water were determined to know a possibility of grading seeds. Dead seeds of lettuce leaked significant amounts of total sugars, amino acids, and proteins, while high quality seeds leaked negligeable amounts of total sugars and some of amino acids and proteins. Dead seeds of welsh onion leaked significant amounts of amino acids and some total sugars and proteins, while high quality seeds leaked negligeable amounts of these compounds. Sesame seeds leaked little total sugars, amino acids, and proteins regardless of seed quality.

• Horticultural Science & Technology
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• v.32 no.1
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• pp.26-32
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• 2014

We investigate the effect of post-conditioning periods and seed orientation on the vigor of cucurbit seeds with dry heat treatment (DHT). All the dry-heat treated seeds exhibited varying degree of seed vigor decreases. In general, pumpkin seeds showed less vigor decreases than the bottle gourd seeds. When the dry heat treated seeds were germinated after post-conditioning for 0, 30, and 120 days, the percentage of germination was enhanced by increasing the period of post-conditioning and the efficiency of post-conditioning differed by crop and cultivar. In both bottle gourd and pumpkin, the vigor of seeds placed in vertically upward and horizontal orientations was higher than that of the seeds placed in the vertically downward orientation. The results suggested that the vigor of dry-heat treated seeds could be improved by applying the proper post-conditioning and seed orientation.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2022.09a
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• pp.71-71
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• 2022

들깨는 잎은 채소, 종자는 기름과 향신료로 이용하는 특용작물로 국립농업과학원 농업유전자원센터에는 국가등록 3,066자원을 보유하고 있다. 들깨 유전자원의 보존기간과 방법에 따라 종자 활력의 차이가 어떻게 변화했는지 알아보기 위해 농업유전자원센터 보존 중인 들깨 유전자원의 발아율을 조사하였다. 온도는 4도, -18도, 밀폐용기 보관하여 보존한 들깨 종자를 10년 보존 후 발아조사를 수행하였다. 발아환경은 페트리디쉬에 거름종이를 깔아 멸균수로 수분을 공급하고, 종자를 50립씩 2반복 치상 후 20도에서 7일, 14일에 조사하였다. 4도에서 보존한 들깨 577자원은 발아율이 85%이상인 자원이 184자원으로 나타났다. -18도에서 보존한 들깨 725자원은 발아율이 85%이상이 자원이 329자원으로 나타났다. 세계적으로 식물유전자원은행에서 양호한 발아율의 기준으로 85%를 사용하는데, 4도 보존 들깨 유전자원들은 조사한 자원의 32%가 양호하였고, -18도 보존 자원들은 45%가 발아 기준을 만족하였다. 들깨유전자원을 10년 주기로 발아율을 조사한 결과 -18도 보존 종자의 발아율이 4도 보존 종자보다 기준발아율이 13% 우수하였으나 두 처리 모두 85% 기준발아율을 만족하는 자원이 50%이하로 나타났다. 본 연구결과는 들깨유전자원을 4도, -18도에서 장기 보존할 때에 종자 활력 예측 및 활력 모니터링 연구에 도움이 될 것이다.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.36 no.1
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• pp.65-69
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• 1991

In order for investigating the factors related to decrease of seedling vigor in the naked barley lines, two naked lines and a covered variety were sprouted with 20 mm rainfall in the natural condition, dried and the sprouted seeds were threshed by hand and small machine thresher, Chiyota. In the case of hand-threshing of the sprouted seeds, the germination rate index (GRI), germination rate, and TTC test value of the different varieties were not different due to the different sprouting degrees, but emergence rate index (ERI) became decreased with stronger sprouting damage. In comparison with hand-threshing, the embryos of sprouted seeds from machine threshing with 700 rpm were partially damaged, and the traits related to seedling vigor were decreased highly significantly. However, the diastatic activity was sharply increased by stronger sprouting and machine threshing. The sprouting degree in the hand-threshing plot was significantly correlated with the diastatic activity(r=0.931＊＊, 0.951＊＊) and GRI(r=0.461), respectively.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.3
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• pp.314-321
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• 1995

Experiments were conducted to obtain information on separation of nonviable seeds from seed lots of rape, Chinese cabbage and radish, by nondestrutive ways. Seeds were artificially aged at 90% relative humidity(RH) and 45$^{\circ}C$ to get different seed qualities. Large amounts of amino acids, proteins and sinapine were leaked from the dead seeds into water in the course of 4~8 hours soaking, while high quality seeds did not leak in all crops. Percentage of normal seedlings from nonfluorescent cellulose coated seeds of rape, Chinese cabbage and radish under ultraviolet light was 96, 96 and 74%, while that of fluorescent seeds was 8, 9 and 1%, respectively. Cellulose coating and storage of Chinese cabbage and rape seeds at 60 and 75% RH for 3 months and 90% RH for 2 months did not deteriorate seed quality. But percentage of normal seedlings from nonfluorescent radish seeds stored at 60, 75 and 90% RHs for 3 months was 63, 64 and 2%, respectively.

• Korean Journal of Environmental Biology
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• v.35 no.2
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• pp.207-214
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• 2017

There is little information about the seed longevity of wild plants, although seed bank storage is an important tool for biodiversity conservation. This study was conducted to predict the seed viability equation of Agastache rugosa. The A. rugosa seeds were stored at moisture contents ranging from 2.7 to 12.5%, and temperatures between 10 and $50^{\circ}C$. Viability data were fitted to the seed viability equation in a one step and two step approach. The A. rugosa seeds showed orthodox seed storage behaviour. The viability constants were $K_E=6.9297$, $C_W=4.2551$ $C_H=0.0329$, and $C_Q=0.00048$. The P85 of A. rugosa seeds was predicted to 152 years under standard seed bank conditions. The P85 predicted by seed viability equation can be used as basic information for optimization of seed storage processes.

• Journal of Korean Society of Forest Science
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• v.111 no.3
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• pp.418-427
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• 2022

Our aim was to evaluate the quality of pine seeds after long-term storage and to analyze the correlation between germination and physiological characteristics. Therefore, we investigated the germination and physiological characteristics of seeds stored for different periods and storage temperatures. Pine seeds lost little viability and vigor after 12 years of storage at 4℃; it is even possible to prolong the storage period at -18℃. The deterioration process operating through long-term storage was characterized by a loss of seed vigor before a loss in seed viability. Electrical conductivity of the seed leachate was significantly higher in seeds that completely lost their viability, and was also found to be significantly correlated with the germination percentage, T50, mean germination time, and germination speed. Among the minerals leaked to the leachate, K had the highest concentration, followed by Na, Ca, Cu, Mg, and Fe, while Mn and Zn were not detected. Concentrations of K, Ca, Cu, Mg, and Fe in the leachate differed significantly by treatment, and in the cases of K, Ca, Cu, Na, and Mg, we observed large differences in the leachate between the seeds that were collected in 2003 and those collected in other years.. Germination percentage was significantly negatively correlated to the concentrations of Ca, Cu, K, Mg, and Na. The coefficient of uniformity of germination was significantly negatively correlated to Cu concentration. The other germination characteristics were not significantly correlated with the mineral concentration. Therefore, the mineral concentrations of pine seed leachate were not reliable indicators of seed deterioration when the differences in quality between seed lots were small. However, electrical conductivity could be an indicator of seed viability and vigor of pine seeds under long-term storage.

• Korean Journal of Agricultural and Forest Meteorology
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• v.8 no.1
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• pp.10-14
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• 2006

We tested the seed viability of Pinus rigida using accelerated aging to discover optimum times and temperatures far artificially accelerated aging. Seeds were artificially aged at different temperatures and during different tines. The seed viability was affected by the accelerated aging and by temperature with a decline in germination and seed vigor. The aging index of P. rigida seed was 0.31 at $35^{\circ}C$ and seed viability was nearly lost after aging treatment at $40^{\circ}C$ for 15 days. The optimum temperature of P. rigida far the aging test was decided to be approximately $37^{\circ}C$ on the basis of the aging index. Inorganic materials and conductivity of leaching solution from aging seeds increased with the increase of aging period. The accelerated aging test was considered to be a suitable method to evaluate the seed viability of tree species. Because seed characters are much different among tree species, however, more studies need to be done to discover the optimum conditions for aging by tree species.

• Korean Journal of Environment and Ecology
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• v.29 no.4
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• pp.503-507
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• 2015

To examine the interannual variation of seedfall and seed viability rates of Acer pictum subsp. mono (APSM), we investigated the annual seedfall and seed viability of APSM in the natural broadleaved forest in Mt. Jungwang, Gangwon-do, from 2009 to 2014. The amounts of annual seedfalls from 2009 to 2014 were revealed to be 5,700, 4,060, 60,900, 403,450 and 310 ea/ha respectively. 2013 is presumed to be the masting year since large amounts of seedfall were revealed, compared to that of the other years in the study period. Also, the amount of seeds was significantly different among seed viabilities. Most fallen seeds were damaged or decayed, accounting for 47.88% of the total, followed by empty seeds, undeveloped seeds and sound seeds in that order. The percentage of sound seeds ranged from 6.7 to 31.0%, which marks the lowest average with 16.74%. In the masting year, 2013, a significantly larger number of sound seeds fell at 125,070 ea/ha, on the forest floor than in the other years (320~17,840 ea/ha). Meanwhile, the ratio of damaged or decayed seeds was the lowest at 38%, although the number of those seeds was the largest, 153,310 ea/ha, for the same year. These results imply that the surplus seeds are supplied for natural regeneration in the masting year, supporting the seed predator satiation hypothesis. More long-term researches should be conducted on the seed production of broadleaved trees.