• KOREAN JOURNAL OF CROP SCIENCE
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• v.33 no.1
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• pp.54-58
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• 1988

To define natural embryo growth and germination of the Korean ginseng (Panax ginseng C.A.Meyer) seed, freshly harvested and depulped seeds were seeded on nursery bed at Aug. 5 without stratification. At 100 days after seeding, embryo/endosperm length ratio of the seeds in nursery bed was higher than that of the seeds which were stratified with conventional method for 100 days. And also there was no significant difference between dehiscence ratio of the seeds in nursery bed and that from stratification with sand. The germination ratio of the ginseng seeds seeded without stratification at Aug. 5 was about 57% in average of 2 years and was not significantly lower than that of the seeds seeded after stratification at Nov. 15. And there were also no significant differencies of plant growth after germination between the ginseng seedlings from two seeding methods.

• Journal of Bio-Environment Control
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• v.22 no.1
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• pp.61-64
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• 2013

This study was conducted to investigate the general characteristics of raw okra seeds and the functional components of roasted okra seeds. The number of okra seed per pod was 78 in 'Greensod' and 88 in 'Beny'. The weight of okra seed per pod of 'Greensod' and 'Beny' were 4.4 g and 6.3 g, respectively. Free amino acid contents of the stir-fry and fresh okra seeds were measured as $2.69mg{\cdot}g^{-1}$ and $0.31mg{\cdot}g^{-1}$. Total polyphenolic compound content of the stirfry okra seeds was estimated as $12.61mg\;CGA{\cdot}g^{-1}$, compared to $2.54mg\;CGA{\cdot}g^{-1}$ fresh okra seeds, Thus, free amino acid and total polyphenolic compound contents in the stir-fry okra seeds were higher than fresh one, Antioxidant activities, such as DPPH and ABTS radical scavenging in the stir-fry okra seeds was the higher than fresh okra seeds.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2023.04a
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• pp.10-10
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• 2023

투구꽃속(Aconitum L.)은 미나리아재비과(Ranunculaceae)의 가장 큰 속(Genus) 중 하나로 300-400 여종(Species)이 북반구 온대지역에 분포한다(Tamura, 1993). 둥근 투구모양의 꽃받침이 특징적이며, aconitine과 같은 알칼로이드 성분을 함유하고 있어 전통적으로 약용소재로서 활용되었다(Khorana, 1968). 세뿔투구꽃(Aconitum austrokoreense Koidz.)은 한국 경상도, 전라남도에만 국지적으로 자라는 한국 특산식물이자, IUCN에서 CR(Critical Endangered) 등급으로 지정된 희귀식물이다. 이 종은 다년생이지만, 서식지에서 개체수가 적거나 유묘의 발견이 어려운 등 자생지 축소 및 절멸의 우려가 높다. 세뿔투구꽃을 보호하고 종을 보존하기 위해서는 종자 발아 · 휴면에 대한 지속적 연구와 종자번식법의 개발이 필요하다. 본 연구에서는 세뿔투구꽃의 종자의 휴면유형을 분석하고 호르몬에 의해 휴면타파를 유도하였으며, 특히 발아 2단계에서 일어나는 생리적 변화를 단백질체학적 관점에서 분석하였다. 세뿔투구꽃 종자는 미발달된 배를 가지고 있으며, 충분한 수분과 온도의 조건에서도 발아가 관찰되지 않아 형태 · 생리적 휴면으로 판단되었다. 종자는 BAP 처리에서 휴면이 타파되는 것을 확인하였으며, 특히 BAP 농도에 따른 발아율은 통계적으로 유의한 값을 가졌다 (F=23.208, P<0.01). 건조된 종자에 비해 BAP 처리된 종자는 발아 2단계에서 몇가지 단백질의 증가가 관찰되었다. 대부분의 종자는 30S ribosomal protein subunit으로, 휴면상태에서 발아상태로 변환이 일루어지는 생리적 변화의 증거로 확인된다. 특히 발아2단계를 지나는 세뿔투구꽃 종자에서는 cytochrome subunit과 photosystem II protein의 급격한 증가가 관찰되었는데, 이는 배아의 발달 결과인 자엽이 발아 즉시 광합성이 가능하도록 준비하는 과정에서 발현 된 것으로 추정한다. 본 연구는 발아2단계에서 휴면타파에 직접적인 영향을 미치는 요인을 발견하지 못하였으나, 휴면타파에 중요한 단계인 발아 2단계 종자의 생리학적 변화를 이해하기 위한 기초자료로 활용 될 수 있을 것이다.

• Korean Journal of Environmental Agriculture
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• v.15 no.1
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• pp.86-90
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• 1996

This study was to provide the basic information for the development of thermal weeder which uses LPG as fuel. Weed seeds of Digitaria sanguinalis S. and Portulaca oleracea L. mainly developed in fram and forestry nurseries were used as experimental samples. At different irradiation temperature(60, 80, 100, 150, $200^{\circ}C$), the dffects of weed seed species(digitalis, purslane), condition of seed(dry, soaked), and irradiation time(2, 5, 10, 20, 30, 60, 180, 300 sec) on seed viability were investigated was investigated by examining interaction and main effect of experimental factors. The results showed that viability of weed seed was significantly affected by all irradiation temperature tested. Irradiation time significantly affected on viability of weed seed on all levels of irradiation temperature. also, there were interactions between condition of weed seed and irradiation time on seed viability at each irradiation temperature.

• Korean Journal of Plant Tissue Culture
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• v.21 no.3
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• pp.183-186
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• 1994

Somatic embryos derived from cell suspension cultures of rice were singly alginate-encapsulated to be used as artificial seeds. When placed on half strength MS solid medium,73% of the encapsulated somatic embryos were capable of germination Encapsulation per se did not affect the germination frequency of embryos. When incubated by wrapping with moistured non-sterile filter paper, 60% of the encapsulated somatic embryos germinated. However encapsulated zygotic embryos without endosperm showed a high germination frequency regardless of the sterility of the incubation conditions. The results suggest that a greater susceptibility of somatic embryos to contaminants is attributed to lower germination frequency of encapsulated somatic embryos in non-sterile conditions.

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2004.05a
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• pp.137-140
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• 2004

채종원관리를 효율적으로 관리하기 위하여 시설되는 저 규격도로는 대부분 비포장상태로 이용되는 경우 많다. 이는 강우에 의한 침식붕괴로 하류의 산림식생환경 및 수질환경 오염으로 연결될 위험성이 내포되어 있어 적절한 방법으로 사전에 방지할 필요가 있다. 본 연구에서는 환경과 친화적인 재료를 최대한 이용하여 개발된 목배수로를 내구성 강화를 검토하였던바 섬유질 포의 조절과 고무질의 성능 조절에 의한 검토로 현장에의 적용이 가능하였다.

• Journal of Ginseng Research
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• v.25 no.4
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• pp.167-170
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• 2001

Experiments were conducted to study the optimum chilling period for breaking physiological dormancy of dehisced ginseng(Panax ginseng C. A. Meyer) seed. Embryo of ginseng seed is too small to be noticed with naked eyed on harvesting time. Embryo grew to half size of endosperm after seeds were stratified for 3 months. It takes 6 months for this embryo to reach the size enough for germination. And it grew faster indoors than outdoors. Dehisced ginseng seed with full-size embryo did not germinate at room temperature and required chilling treatment for 75days in outdoors and 90 days in cold chamber. While seed receiving sufficient chilling treatment were left to be in room temperature, the chilling effect decreased remarkably.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.33 no.1
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• pp.59-63
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• 1988

Freshly harvested and depulped Korean ginseng seeds were subjected to the seed treatment of removing endocarp plus surface sterilization with sodium hypocloride, surface sterilization only, and nontreated control. These seeds were stratified at temperatures of 5$^{\circ}$, 10$^{\circ}$, 15$^{\circ}$ and 20$^{\circ}C$ for 20, 40, 60, 80 and 100 days. Embryo growth of the ginseng seeds of which endocarp was removed was most rapid in each stratification temperature and that of sterilized seeds was slower than unsterilized seeds after 80 days stratification at 15$^{\circ}$ and 20$^{\circ}C$. About 15$^{\circ}C$ was an optimal stratification temperature for embry growth in ginseng seeds. Chilling treatment at 5$^{\circ}C$ for 100 days was needed for better germination of dehisced ginseng seeds. An optimal germination temperature for the ginseng seed following chilling treatment was about 15$^{\circ}C$.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.31 no.1
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• pp.19-23
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• 1986

To get the basic information about ginseng seed production, yield of seed and seed quality in 27 fields were investigated. Yield of seed ranged from 4 to 10 litres per 100 kan (180cm x 90cm). Ratio of under 4mm seed is 12%, 66% for 4 to 5mm and 22% for over 5mm. Seed weight and ratio of over 4mm seed showed the decreasing tendency with the increase of seed yield per unit area. The seed yield harvested from the selected mother plants was lower than that from all plants, but seed weight and ratio of over 4mm seed were high in fields using the selected mother plants. It showed a positive correlation between length and width of seed, but no correlation between thickness and length or width. Optimum yield for high quality seed appeared under the 7 to 8 litres per 100 kan.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.26 no.2
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• pp.157-166
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• 1981

Laboratory experiements were conducted to evaluate water uptake and osmotic potential of soybean seeds associated with germinability. Bonus, Wayne, Essex and Pickett were selected for this study. Large and small seeds from the four varieties were included in water uptake measurements at $25^{\circ}C$ There were significant differences in water uptake during germination due to seed size and cultivars at optimum moisture condition, -6 and -15 bars soil moisture. As water stress increased, the water uptake of Pickett and Essex were superior to Bonus and Wayne, and small seeds were superior to large seeds. The seed moisture content at germination was 60.8% on a fresh weight basis under optimum moisture condition. The minimum moisture contents necessary for the germination were 50.2% at -6 bars and 50.9% at -15 bars. There were significant differences among varieties in seed osmotic potential during germination, although these differences depended on imbibition time. The average osmotic potential ranged from -32.0 bars after 4 hours imbibition to -11.2 bars at the beginning of germination. The correlation coefficient between seedling length and osmotic potential was not significant after any period of imbibition, suggesting that osmotic potential is not directly associated with seedling growth. However, osmotic potential is closely related to water uptake capacity of soybean seed.