• Horticultural Science & Technology
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• v.33 no.3
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• pp.448-452
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• 2015

'Seeberry' is a short-day $F_1$ hybrid strawberry for seed propagation, that was released by the Protected Horticulture Experiment Station (RDA, Republic of Korea) in 2013. Inbred lines derived from domestic germplasms and selected varieties were used to develop this cultivar. 'Seeberry' originated from a cross of two inbred lines: a male parent 'Wongyo 3115' with high firmness and a female parent 'Wongyo 3116' with excellent fruit shape and high yield. 'Seeberry' cultivar should be grown with a crown diameter of over 10 mm to reveal its unique characteristics. 'Seeberry' has an upright plant shape with elliptic leaves, 12-15 flowers per cluster, vigorless growth habit, and average flower bud differentiation compared to vegetatively propagated cultivars. Fruits of 'Seeberry' are conical, having light skin color and approximately 15-16 g in average weight. Yield of this cultivar is about 92% lower than that of 'Akihime', but 'Seeberry' has excellent taste, sugar content of $9.7^{\circ}Bx$, acidity of 0.6%, and good texture. With regard to disease and pest resistance, 'Seeberry' is sensitive to powdery mildew, aphids, and the two-spotted spider mite, and is resistant to anthracnose.

• Journal of Wetlands Research
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• v.16 no.1
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• pp.11-18
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• 2014

The effect of elevated $CO_2$ and temperature on ecological characteristics of Cicuta virosa L., the endangered plant were examined under ambient $CO_2$+ambient temperature(AC-AT), ambient $CO_2$+elevated temperature(AC-ET) and elevated $CO_2$+elevated temperature for two years. Shoot length and the number of umbels were not different in three environmental gradients. The number of tillers was high in the order of EC-ET, AC-ET and AC-AT. The number of compound umbel was the lowest in the EC-ET. Fruit set rate was the highest in the AC-AT. These results mean that unsexual propagation of C. virosa may increase by promoting growth of tillers, rather than seed production under future global warming. This population growth study will be used as the important data for the research of Korean endangered species.

• Korean Journal of Medicinal Crop Science
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• v.12 no.3
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• pp.209-213
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• 2004

Ligusticum chuanxiong is receiving much attentions as one of the important medicinal crops with the increasement of the crude drug demands. This study was conducted to obtain the basic informations of breeding of Ligusticum chuanxiong. Embryological characteristics were examined to elucidate the process of male and female gametophytic development and fertilization. Meiosis and nucleus division of megaspore and microspore were proceeded normally. With regard to the formation of female gametophyte, only a half of female gametophyte developed to normal egg apparatus. While another 50% showed abnormal egg apparatus with poly-nuclei or non-nuclei ovule. The pollens developed from the micros pore were formed more than 90 % of normal pollen. It was difficult to observe fertilization because ovule tissue was very compact and cell was extremely tiny, but could be easely observed proembryo and embryo formation. Only 30 percent developed into proembryo and subsequently into embryo, and the others were degenerated.

• Korean Journal of Plant Resources
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• v.30 no.4
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• pp.372-377
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• 2017

This research was performed to develop mass propagation method of Alpine modest primrose (Primula modesta var. hannasanensis T.Yamaz.) for improving the conservation and utilization of the species. Seeds were collected on August 2011 in Jeju-city and well-selected seeds were dry-stored at $4{\pm}1.0^{\circ}C$. Seed size ranged $0.44{\pm}0.07{\times}0.61{\pm}0.04mm$, and weight of 1,000 seeds was $51.78{\pm}0.021mg$. Thus the seed was classified as 'dwarf seeds'. As the result of dormancy characteristics, moisture content of freshly matured seeds increased rapidly by water-soaking treatment and seeds did not germinate at $20^{\circ}C$ for 4 weeks under the light condition. Therefore, seeds were estimated to have physiological dormancy. Germination conditions of dry-stored seeds were found to be $20^{\circ}C$ and light condition, and seeds did not germinated under dark condition regardless of all temperature regimes. Therefore, Alpine modest primrose seed was considered as photoblastic type. Percent germination (PG) and germination energy (GE) were greatly improved by soaking the seeds in $GA_3$ and kinetin for 24 hours. Especially, 200~500 mg/L $GA_3$ treatment resulted in the highest PG (95.5%) and GE (98.3%). So, chemical treatment such as $GA_3$ was thought to be a useful method for raising seedling uniformly.

• Journal of Plant Biotechnology
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• v.30 no.1
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• pp.41-45
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• 2003

A study was under taken to investigate the appropriate explant sources of flower organ and suitable cultural conditions such as light, temperature, and sucrose in plant regeneration of Iris ensata culture. Explants of perianth, ovary, pedicel, and peduncle of Iris ensata were cultured at different daylength (0, 8, 16, 24 hour), different temperatures (10, 15, 25, 3$0^{\circ}C$), and sucrose concentrations (1, 3, 6, 9%) on MS medium. Formation of adventitious roots from explants of Iris ensata was effective in the dark, while that of adventitous shoots was effective in the light. The optimum daylength for young plant regeneration was 16 hours. The optimum temperature for shoot formation of Iris ensata explants was $25^{\circ}C$ but the formation at 10 and 15$^{\circ}C$ was ineffective. Especi-ally, perianth and ovary was effective in shoot formation from flower organ expants. T-he optimum concentration of sucrose for shoots and roots formation of Iris ensata explants was 3 and 6%, respectively.

• Journal of Korean Society of Forest Science
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• v.28 no.1
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• pp.36-42
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• 1975

In the beginning of the germination of Castanea creata seeds, seedlings with identical genetic characteristics from only one seed were produced by longitudinally splitting the radicle and epicotyl in half with a razor blade. The results are summarized as follows: 1. 2-8 genetically identical Castanca crenata seedlings were produced by longitudinally splitting the radicles and epicotyles from one to four in number in half with a razor blade 1-3 times about 7 days apart, while the seeds were germinating. 2. The proportion of success of splitting seedlings was very high (90-100%). When some of the spliting seedlings were withered a sprout appeared again and it was possible the seedling to revive. 3. The production rate, growth and T/R rate of seedlings which were split only once were lower that those of the seedlings which were split several times. 4. The growth and T/R rate of split seedlings which were one of no more than four seedlings produced from one seed showed no difference in comparison with normal seedlings, but in seedlings which were one of more than four, the growth and T/R rate were considerably decreased. 5. With the progress of growth, the external shapes of splitting seedlings completely returned to the original state, and no problems in growth, were found.

• Horticultural Science & Technology
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• v.34 no.3
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• pp.461-471
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• 2016

This study was conducted to establish an in vitro propagation system for sea-milkwort (Glaux maritima L.), which is an endangered coastal plant species with high horticultural value. Two phenotypes, 'Red type (RT)' and 'Pistachio type (PT)' based on the colors of stem and flower, were obtained from a personal horticulturist in 2009 and used for this study as plant materials. The stock plants showed typical morphologies in flower, capsule, and seed appearances as previously reported. Low temperature treatment at $4^{\circ}C$ for four or more weeks after in vitro sowing maximized seed germination percentage, indicating that imbibition of seed and subsequent low temperature treatment are crucial for its germination. The in vitro seedlings had phenotypic variation, falling into 'RT' and 'PT' classes like the stock plants. Although slight differences depending on genotype and medium were recognized, the fourth or fifth nodes detached from the in vitro seedlings revealed the best multiplication efficacy when estimated on the basis of total number of nodes of newly developed axillary shoots. In addition, the nodes from 'RT' and 'PT' regenerated the most shoots on medium supplemented with $0.5mg{\cdot}L^{-1}$ BA alone and $0.5mg{\cdot}L^{-1}$ BA plus $0.5mg{\cdot}L^{-1}$ IAA, respectively. The node culture-derived plantlets were well acclimatized in a culture room ex vitro and completed the pseudo-annual life cycle coincident with that in the natural salt march habitat with the current cultivation method of applying fresh water-irrigation under an inland environment. This work represents the first report of in vitro propagation of sea-milkwort. Thus, our study will contribute to exo-habitat conservation and natural habitat restoration of this endangered species in addition to development of a horticultural product.

• Korean Journal of Plant Resources
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• v.27 no.5
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• pp.534-539
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• 2014

This study was carried out to establish seed propagation method of Hydrangea petiolaris Siebold & Zucc., one of the specific and protected plant species for the floral region. Seed size ranged $1.36{\times}0.84mm$, weight of thousand seeds was $2.3{\pm}0.02mg$. Observation of seed morphology characters showed wrinkled surface and elliptical shape, indicating amber color and fine seeds. Moisture contents of seeds, increased rapidly by water-soaking treatment, recording maximum moisture contents (31.9%) after 48 hours. Percent germination of seeds was higher under the light than dark condition. Seed germination was the best at $25^{\circ}C$ under the light condition (78.0%) among temperature and light conditions treated. Percent germination, germination energy and $T_{50}$ was greatly improved by soaking in $GA_3$ solutions for 48 hours. Especially, $100{\sim}500mg{\cdot}L^{-1}$ $GA_3$ treatment resulted in the highest germination rate as 90.0% and above.

• Korean Journal of Plant Resources
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• v.37 no.1
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• pp.79-86
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• 2024

This study was conducted to determine the conditions for breaking dormancy in the seeds of the North Korean plant resource, Trollius macropetalus, and to investigate its germination characteristics. The morphological characteristics and viability of T. macropetalus seeds were examined. Germination characteristics were investigated based on temperature conditions (15/6℃ & 25/15℃), cold stratification treatment, and GA₃ treatment. The results showed that cold stratification treatment had no effect on breaking the dormancy of T. macropetalus. GA₃ treatment significantly increased the germination rate of T. macropetalus and also improved the mean germination days and germination speed. However, compared to the 15/6℃ condition, the effectiveness of GA₃ treatment for T. macropetalus decreased under the 25/15℃ condition. It was determined that T. macropetalus have a nondeep-type morpho-physiological dormancy. The most effective conditions for breaking dormancy were found to be the 15/6℃ temperature condition and GA₃ treatment at 500 mg·L^-1. The findings of this study are expected to provide valuable information for the mass propagation of T. macropetalus, which has potential applications in landscaping and pharmaceutical materials.

• The Inventors News
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• no.54
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• pp.1-16
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• 2007

한국여성경제단체연합, 여성경제계 위해 공식 출범/발행인 칼럼/'마드리드 출원제도' 적극 활용 상표 침해 줄여라/일본 특허출원 심사기간 단축된다/기업.연구소 신입사원75%, 특허 관련 지식 전무/'주몽'이계인 씨 산자부 홍보대사 위촉/특허등록세, 신청 다음날 내도 된다/특허청, 6시그마 공공부문 혁신 주도/아듀 2006 한국여성발명협회 결산/자문위원 칼럼/로봇시장에도 브랜드 선점 열풍/농산물도 발명특허 상표 등록 시대/도소매업도 서비스업으로 출원 가능/기름대신 물 사용한 자동차, 항공기 현실화/반도체 배선 기술 특허 출원 상승세/자동차는 모바일 엔터네이너/'적외선 감지기술' 우리 생활속으로/서울반도체.바론테크, 일본 특허분쟁 승소/하나TV, 특허권 침해 피소/삼성전자, 분쟁.경쟁 대응위해 특허조직 통합/신한다이아몬드 일본 MDI 특허분쟁 승소/미니인터뷰/나노기술 응용한 디스플레이 특허출원 급증/미국의 두 배인 '휴면 특허', 대책 마련 시급/특허법원 "사용횟수 적다고 상표취소 못해"/종자번식 유전자 변형식물도 특허출원 가능/변리사 1차 시험 합격자 대폭 축소/역사 속의 발명품/하루 10분 발명교실/특허Q&A/신년인터뷰/화장하는 방법도 특허 받는다/'취업에서 결혼까지' 올인원 시스템 화제/아이디어 착상 및 발명 기법/아줌마의 힘이 발휘될 때까지 발명 또 발명/버뱅크의 식물 품종 개량/미국 청년, 몸짱 선탠용 티셔츠 발명/스위스, 국가경쟁력 1위의 비밀/저지방식,'유방암' 제발 위험 감소/서울대, 세계최초 암캐 복제 성공/국가 R&D 특허조사, 전체 R&D 부처로 확대/전국 초.중생 발명 글짓기.만화현상 공모전/특허청, 공공부문 성과주의 대통령상/한국여성발명협회 회원사 발명품 가이드