• KOREAN JOURNAL OF CROP SCIENCE
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• v.20
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• pp.100-106
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• 1975

Germinating ability of green seed in maturing period was investigated to develop the technics on generation shortening. Rapeseed did not germinate until 40 days after flowering (=DAF) in green seed and 50 DAF in dried seed. Effective germination to be utilized for generation shortening came on 55 DAF for dried seed by heating and on 75 DAF for green seed. The periodic time of seed dormancy in maturing period had two cycles and rest period was considered that it was different according to the ecological types of early, middle and late maturity.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.26 no.2
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• pp.167-173
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• 1981

Respiration of whole seeds and seed parts isolated from four soybean varieties Essex, Pickett, Wayne and Bonus as measured at $25^{\circ}C$ during germination. The average respiration rates of cotyledons were significantly higher than those of other parts. The values were 35.7, 28.0 and $23.9{\mu}hr^{-1}$ seed$^{-1}$ for cotyledons, embyros, and hila, respectively. Bonus showed a significantly lower respiration rate than the other varieties did. The interaction of variety x seed part was not significant source of variation for respiration. This suggests that seed part and variety independently contribute to soybean seed respiration during early imbibition. The effects of imbibition time and time x variety intereaction on whole seed respiration were significant, but the variety effect was not significant. The correlation coefficient between seed moisture content and respiration after 24 hours of imbibition was significant for Bonus, but not for the other varieties tested. This suggests that Bonus may require more water for respiration during imbibition than the other varieties. There as no significant correlation of whole seed respiration rate after 24 hours imbibition with seed weight or seedling length.

• Journal of Korean Society of Forest Science
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• v.111 no.3
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• pp.418-427
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• 2022

Our aim was to evaluate the quality of pine seeds after long-term storage and to analyze the correlation between germination and physiological characteristics. Therefore, we investigated the germination and physiological characteristics of seeds stored for different periods and storage temperatures. Pine seeds lost little viability and vigor after 12 years of storage at 4℃; it is even possible to prolong the storage period at -18℃. The deterioration process operating through long-term storage was characterized by a loss of seed vigor before a loss in seed viability. Electrical conductivity of the seed leachate was significantly higher in seeds that completely lost their viability, and was also found to be significantly correlated with the germination percentage, T50, mean germination time, and germination speed. Among the minerals leaked to the leachate, K had the highest concentration, followed by Na, Ca, Cu, Mg, and Fe, while Mn and Zn were not detected. Concentrations of K, Ca, Cu, Mg, and Fe in the leachate differed significantly by treatment, and in the cases of K, Ca, Cu, Na, and Mg, we observed large differences in the leachate between the seeds that were collected in 2003 and those collected in other years.. Germination percentage was significantly negatively correlated to the concentrations of Ca, Cu, K, Mg, and Na. The coefficient of uniformity of germination was significantly negatively correlated to Cu concentration. The other germination characteristics were not significantly correlated with the mineral concentration. Therefore, the mineral concentrations of pine seed leachate were not reliable indicators of seed deterioration when the differences in quality between seed lots were small. However, electrical conductivity could be an indicator of seed viability and vigor of pine seeds under long-term storage.

• Korean Journal of Agricultural Science
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• v.8 no.1
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• pp.29-43
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• 1981

The effects of temperature and day length during seed formation on the morphogenesis of lettuce seed were investigated using two cultivars of MSU 15 and MSU 16. Seed size and weight, and thickness of seed coat o f both cultivars greatly influenced by temperature, but fresh weights and dry matter weights of mother plants were influenced by day length, not by temperature. Interactions between the adaptive polymorphism and climatic conditions were also discussed. Environmental conditions affected the morphology of lettuce seed and this variation of seed morphology resulted from interaction between genetic potentialities and environmental conditions.

• Korean Journal of Environment and Ecology
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• v.18 no.1
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• pp.1-6
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• 2004

Seed characteristics-weight, seed length and width, pure seed percentage are measured on 15 native herb species, and seeds of six native herb species were tested on the seedbed in the forest with autumn seeding. Seeds of 13 species including Aster scaber were very small; seed length is less than 7mm and seed width is less than 3mm pure seed percentages of 10 native herb speciesin-cluding Ligularia fischeriwere were much lower than 5％. Germination percent of five native herb species were significantly different among the growing sites. The mean germination percent was highest 47.00％ for Angelica gigas, 38.25％ for Ligularia fischeri, 30.88％ for PimPinella brachycarpa, 15.75％ for Aralia continetalis, 10.00％ for Cirsium setidens, and the lowest 9.32％ for Aster scaber.

• Korean Journal of Agricultural Science
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• v.9 no.1
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• pp.275-283
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• 1982

One hundred and sixteen seed samples of 16 different crops were tested by routine seed health testing methods. Seeds of rice, soybean and barley were found to be associated with 18, 15 and 7 species of fungi, respectively. Discoloration and spotting of various kinds were observed in rice seeds. Epicoccum purpurascens, Pyricularia oryzae and Trichoconiella pedwickii were higher in these discoloured than in normal seeds of rice. Macrophomina phaseolina, the charcoal-rot fungus is a new record for soybean in Korea. Seeds of sesame were infected by 13 fungi Alternaria sesami, A. sesarmicola and Corynespora cassiicola were detected predominantly. Twenty one species of fungi were detected in seed samples of 12 commercial vegetable crops. Alternaria, Colletotrichum and Fusarium were found predominantly. Some seed samples of commercial vegetable crops were heavily infected with pathogenic fungi and showed heavy seed and seedling rot.

• Proceedings of the Korean Society of Organic Agriculture Conference
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• 2009.12a
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• pp.297-297
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• 2009

최근 유기농산물에 대한 관심과 요구가 급증하면서 소비자들은 고품질 유기농산물을 선호하고 있어 국내 대부분 유기농가들은 무처리 일반종자를 이용하고 있는 실정이다. 앞으로 국제 기준에 부합하는 채소류 유기재배를 위해서는 유기종자의 이용이 필수적이므로 유기종자 채종체계 확립이 필요한 실정이다. 따라서 본 연구에서는 당근 종자(재래종 heimloom)를 사용하여 유기종자의 채종체계를 확립하고자 하였다. 일반적으로 당근종자 생산은 적어도 2년을 요구한다. Seed to seed 방법과 Root to seed 방법이 이용되고 있는데 본 연구에서는 Root to seed 방법을 사용하였다. Root to seed 방법은 6월에 당근종자를 파종하여 가을에 뿌리를 수확하여 지상부를 5cm 정도 남기고 정지하여 일시 저온저장 하였다가 이듬해 뿌리를 이식하여 새순에서 추대시켜 종자를 수확하는 방법으로 종자 생산성이 높아 종자회사에서 많이 사용하고 있다. 본 연구에서도 Root to seed 방법으로 채종 시험하였다. 2008년도에 파종 및 수확한 뿌리를 저온저장고에 일시 저장 후 1차 이식은 2008년 11월 하순에 노지포장에서 실시하고 이때 월동 중 동해를 막기 위하여 보온덮개로 멀칭 처리하였다. 이듬해 3월 중순에 주간거리 75${\times}$40cm 간격으로 솎음하여 처리당 10주씩 배치하였다. 이식후 뿌리에서 새순 1대를 유도하였고 추대 및 개화 6주후부터 종자가 갈색으로 변하기 시작할 때 채종을 시작하였다. 수확부위는 1차 측지 중 1번째 화지를 대상으로 하였고 화경절단은 화총으로부터 20cm 정도 남겨서 잘랐고 실내에서 1개월 이상 충분히 후숙시킨 후 종자를 정선하여 종자생산성을 조사하였다. 결실율과 발아율 조사는 채종된 종자를 이용하여 종자 충실도와 발아력을 조사하였다. 각 품종별 추대율은 15번 품종과 16번, 24번 품종이 5% 미만으로 매우 부진하였고 그밖에 추대된 묘의 생육 및 발육상은 매우 양호하여 개화에는 커다란 문제가 없었다. 종자 결실율은 23번 품종을 제외한 모든 품종에서 높았고 각 품종별 화총구조를 고려해 볼 때 주간 화총, 1차측지, 2차측지 순으로 결실율이 떨어졌다. 각 품종의 천립중은 1, 11, 12, 14, 20번 품종이 2.02g 이상으로 대립종으로 판단되며 각 품종별 화총의 크기는 비슷하여 대립종은 각 화총당 종자수가 적은 것으로 나타났다. 대분분의 대립종은 만생종으로 생육기간이 소립종 보다 10-20일상 길며 종자 성숙기간도 장기간 소요되며 특히 장마기간을 넘겨야 할 경우에는 비가림 시설 채종재배가 필수적인 것으로 나타났다. 발아율은 10, 11, 14, 18, 19, 26번 품종이 80% 이상으로 가장 높았고 다른 품종에서는 다소 떨어져 품종별 차이가 많았다. 종자 생산성은 장근종과 단근종간에 약간의 차이가 있지만 주간 화총을 포함하여 1차 측지 4-5개에서 채종하는 것이 가장 효율적인 것으로 사료된다. 또한 당근의 개화 기간을 보면 1화륜은 8~10일, 1개체는 40~50일, 채종포장에서는 1화륜에서 측지 6개구까지 채종은 60~70일 소요되어 미숙종자와 과숙종자가 혼재되어 적정 수확시기를 결정하기가 매우 어려운 점이 발생하였다. 본 연구 결과에서 생육특성, 수량성, 품질특성, 종자 생산성, 발아율을 고려해 볼 때 1, 7, 8, 9, 10, 14, 19, 26번 품종이 유기농 재배 및 유기채종에 적합한 것으로 사료되었다.

• Korean Journal of Environment and Ecology
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• v.20 no.4
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• pp.436-447
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• 2006

Recently, there is increasing demand on enhancing the efficiency of hydro-seed spraying in afforestation for damaged or degraded land including forest road slopes. In this study, we focus on how seed coating and molding may affect seed germination and seedling growth. Plant species used in the study are Lespedeza cyrtobotrya, Indigofera pseudotinctoria, Arudineila hirta, Poa pratensis, and Lolium perenne. The results of seed germination and seedling growth with and without seed coating and molding are analyzed as follows: 1. For all the species and in both seeding with molding covered with soil and seeding without molding in which seeds were over sown, the increment of germination ratio by seed coating method is greater than by non·coating one. Seed coating increases average germination ratios observed in seeding with molding and without molding by 11.2% and 21.4%, respectively. Germination force may decrease from 0.8 to 3.7 days depending on the plant species and the treatments. The $LD_{50}$ decreased by $0.8{\sim}2.6$ days. However, seed coating delays the start of germination by approximate 1 day for all of the observed plants. 2. Seed coating may have the effect of accelerating the growth of stem and leaf and root. The experimental result shows that seed coating leads to 21.7% and 34.8% increment of average stem and leaf growth by seeding with molding and without molding, respectively. In terms of root growth, seeding with molding results in 22.0% increment while seeding without molding produces 26.2% increased root growth. 3: Compared to seeding without molding, germination starts on an average of 1.3 days later in seeding coated seeds with molding. However. the germination ratio is increased by 5%, and germination force and $LD_{50}$ are observed to shorten by 1.0 day and 1.4 days, respectively. Meanwhile, whether seeds are coated or not may be more related with germination and seedling growth in seeding without molding than with those in seeding with molding. 4. In this study, coating materials are examined to look at which ones are better in each treatment. Coating with Vermiculite+Talcum is the most effective in germination and seedling growth for overall plants. Seed coating using Bentonite, Calcium Carbonate, and Calcium Hydroxide shows better results than non-coating does. 5. When seeds are coated, the greatest enhancement of seed germinations was observed in Indigofera pseudotinctoria and, in the case of seedling growth, Lespedeza cyrtobotrya has the most increasing observation value among the 5 examined species. These results may indicate that woody plant seeds, having greater sizes of seeds than ones of grass seeds, may have greater relation with seed coating than grass plant seeds may have. 6. Therefore, if seeds cannot be molded up after hydro-seeding on forest road slopes, it is recommended that seeds for restoration be pre-coated with Vermiculite+Talcum and then be sowed, in order to quickly stabilize the damaged slope and achieve successful afforestation.

• Horticultural Science & Technology
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• v.31 no.1
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• pp.80-88
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• 2013

Carrot (Daucus carota L. var. sativa) is one of the most extensively used vegetable crops in the world and a significant source of nutrient because of its high content of ${\beta}$-carotene, well known as the precursor of vitamin A carotenoid. However, seed-hairs generated and elongated from the epidermal cell of seeds inhibit absorption and germination by various factors such as carotol and so on. Accordingly, mechanical hair removal process is essential before commercialization of carrot seeds. Because of this process, producers will have additional losses such as time consuming, manpower, capital and so on. Furthermore, physical damage of seeds causes irregular germination rate. To overcome such cumbersome weaknesses, new breeding program for developing hairless-seed carrot cultivar has been needed and studies for molecular markers related to seed-hair characteristic is needed for a new breeding program. Therefore, in this study, cDNA libraries from seeds of short-hair seed phenotype CT-SMR 616 OP 659-1 line, hairy-seed phenotype CT-SMR 616 OP 677-14 line and short-hair seed phenotype CT-ATR 615 OP 666-13 line, hairy-seed phenotype CT-ATR 615 OP 671-9 were constructed, respectively. Furthermore, 1,248 ESTs in each line, total 4,992 ESTs were sequenced. As a result, 19 SNP sites and 14 SNP sites in each of 2 combinations were confirmed by analyzing these EST sequences from short-hair and hairy-seed lines. Then we designed SNP primer sets from EST sequences of SNP sites for high resolution melting (HRM) analysis. Designed HRM primers were analyzed using hairy seed phenotype CT-SMR 616 OP 1040 line and short-hair seed phenotype CT-SMR 616 OP 1024, 1025, 1026 lines. One set of HRM primers showed specific difference between the melting curves of hairy and short-hair seed phenotype lines. Based on this result, allele-specific (AS) PCR primers were designed for easier selection between hairy-seed carrot and hairless seed carrot. These results of HRM and AS-PCR are expected to be useful in breeding of hairless seed carrot cultivar as a molecular marker.

• Journal of Korean Society of Forest Science
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• v.105 no.1
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• pp.42-47
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• 2016

To examine the natural regeneration in the natural mixed-broadleaved forest, flowering, fruiting, seed-fall, and seed viabilities of Acer pseudosieboldianum (Ap) were investigated in Mt. Jungwang, Gangwon-do, from 2009 to 2015. The flower of Ap consisted many male and bisexual flowers on the corymb. Flowering dates are differed between sex morph in the same inflorescence. Stamens are stop growing and disappeared after pollinated pistil begin to grow in bisexual flowers, and male flowers have vestial pistil. The flowers of Ap might be pollinated by Apis mellifera, Andrenidae spp. and Syrphinae spp. Ap had some mechanism to prevent from self pollination with heterodichogamy. Mean annual seedfall of Ap was 70,780 ea/ha (ranged 310~234,840 ea/ha). Annual seedfall of Ap varied severely, and the maximum was about 760 times the minimum. Annual seed production of Ap might be to a normal distribution. Rates of damaged or decayed seeds are highest 59.3%, and those of sound seeds are 23.9%, Those of undeveloped and empty seeds are 9.2% and 7.6%, respectively. The most important factors influencing sound seed production might be the density and activities of insect pollinators and sucking pest in the flowerwing period, middle-late May. Successful regeneration of Ap might be in masting year and on the gap sites with proper conditions to germinate and grow. To understand the natural regeneration of deciduous hardwoods, further study on the characteristics of flowering and fruiting, pre- and post-dispersal seed predation, and annual variation on these factor should be needed.