• Title/Summary/Keyword: 조재원

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Effects of Transforming Growth Factor $\beta$ on In-vitro Maturation of Porcine Oocytes (Transforming Growth Factor $\beta$가 돼지 난자의 체외성숙에 미치는 영향)

  • 신명균;박춘근;조재원;정희태;양부근;김정익
    • Korean Journal of Veterinary Service
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    • v.21 no.3
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    • pp.267-275
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    • 1998
  • 돼지 수정란의 체외생산은 난자의 체외성숙과 체외수정에 관한 기술의 부족으로 아직까지 만족스럽지 못한 수준이다. 특히 돼지 수정란의 체외생산에는 복잡한 세포질의 성숙과정과 높은 다정자침입율 및 전핵형성의 억제등의 문제점이 있다. 본 연구에서는 돼지 난자의 체외성숙 체계를 개선하기 위하여 transforming growth factor$\beta$(TGF$\beta$)의 첨가가 난자 및 난구세포에 미치는 효과에 대하여 검토하였다. 체외성숙용 배지에 TGF$\beta$를 1~10ng/$m\ell$의 농도로 첨가하여 미성숙 난자를 배양한 결과 성숙율이 높아졌다. TGF$\beta$의 효과는 난구세포가 제거된 난자의 성숙에도 효과적이었다. TGF$\beta$(를 첨가하지 않은 배양액 내에서는 배양 24시간 까지 metaphase-II로 성숙된 난자가 관찰되지 않았으나 TGF$\beta$를 첨가한 배양액 내에서는 관찰되었다. 한편, 난구세포가 부착된 난자의 성숙배양시 TGF$\beta$의 첨가시기에 따른 차이는 없었으나, 난구세포를 제거한 난자의 경우에는 성숙배양 전반기(59%) 또는 후반기(57%) 24시간 동안에만 TGF$\beta$를 첨가하는 것이 48시간 동안 계속하여 첨가(27%)하는 경우 또는 비첨가(38%)에 비하여 유의적으로 높은 성숙율을 나타냈다(p<0.05). 이와 같은 결과는 난구 세포가 돼지 난자의 체외성숙에 필수적이지만 TGF$\beta$는 난구세포가 제거된 난자의 체외성숙에 어 느정도 유익한 효과를 발휘하는 것으로 추측된다.

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The Effect of Temperature on Mlcrosensor Chip for the Monitoring of Nitrogenous Compounds(NH4+, NO3-) (질소화합물(NH4+, NO3-)의 모니터링을 위한 마이크로 센서의 작동에 미치는 온도 영향)

  • Lee, Jong-Won;Chon, Kyongmi;Jang, Am;Yu, Hye-Weon;Cho, Jaeweon;Kim, In S.
    • Journal of Korean Society on Water Environment
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    • v.23 no.1
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    • pp.33-37
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    • 2007
  • Microelectrodes for measuring nitrogenous compounds (${NH_4}^+$, ${NO_3}^-$) that were applied into the microfluidics chips was investigated, and the effect of temperature was especially examined. In this specific research, microelectrodes were first calibrated to check the function, and then microsensor that was combined microelectrode with microfluidic chip was re-calibrated. Experimental results showed that there are no change in the function between microelectrode and microfluidic chip. The electro motive force (EMF) for the ${NH_4}^+$ microsensor was similar to the one theoretically calculated from Nernst equation, but the EMF for ${NO_3}^-$ showed minor change.

Hydraulic Model Experiment of Iceharbor-type Precast Fishway (조립식 아이스하버형 어도의 수리모형실험)

  • Kim, Jae-ok;Park, Sang-hyun;Cho, Jae-won;Jo, Guk-hyun;Hwang, Jong-seo;Joh, Seong-ju
    • Proceedings of the Korea Water Resources Association Conference
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    • 2004.05b
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    • pp.893-897
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    • 2004
  • 본 연구는 아이스하버 형태의 어도를 조립식으로 개발하여 실제 현장에 적용하기에 앞서 유량 변화에 따라 어도내 유속 및 유황변화를 조사하고 어도의 배열을 조정하여 어도의 현장적용성을 검토하기 위하여 실시하였다. 모형 월류수심이 40cm(현장 20cm)일 때 상류부는 어류가 소상하기에 적합한 유속을 나타내었고 하류부의 가장 낮은 월류부(좌측열)에서는 어류 소상에 다소 지장을 주는 유속변화를 보였다. 그러나 좌측열을 제외한 우측열과 중간열에서는 어류 이동 및 소상에 적절한 유속변화를 나타내고 있는 것으로 보아 본 어도는 유량 증가에 따라 월류 유속이 증가하여도 월류벽이 높은 구간에서는 유속이 증가폭이 크지 않기 때문에 수위변화에 대응력을 가지는 것으로 판단된다. 다양한 배열로 어도를 설치하면 유속이 느린 어도 구간은 어도 유인수로로서의 역할을 할 수 있으므로 어도내의 다양한 유속은 어류 소상에 유리하게 작용한 것으로 평가된다. 어도의 배열은 월류부의 높이가 높은 것을 좌${\cdot}$우측열에 배열하고 낮은 것을 중간열에 배열하면 갈수량에 대한 대응력이 증가하는 것으로 나타났다. 잠공은 하천의 유량 조건이 좋을 때보다 갈수기나 홍수기에 어류가 소상하는 통로의 역할을 할 수 있을 것으로 판단된다. 따라서, 본 어도는 다양한 월류벽의 배열로 하천의 수위변화에 대한 대응력을 가지고 있으며 구조적으로 견고하여 우리나라 하천 상황에 적절한 어도로 판단된다.

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Hydraulic Model Experiment for Field Application of Iceharbor-type Precast Fishway (조립식 아이스하버식 어도의 현장 적용을 위한 수리모형실험)

  • Kim, Jae-Ok;Park, Sang-Hyun;Cho, Jae-Won;Hwang , Jong-Seo;Jo , Guk-Hyun;Joh , Seong-Ju
    • Journal of The Korean Society of Agricultural Engineers
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    • v.46 no.2
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    • pp.3-14
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    • 2004
  • This study was conducted to assess the possibility of the field application of the iceharbor-type precast fishway. When overflow depth of weir is 4.0 cm in model fishway, upper part velocities appear appropriate for upstream migration of fish and the lowest overflow wall (right line) in lower part has shown velocity distribution more or less inadequate for upstream migration. Except that right line, left and middle line revealed that velocities are appropriate for upstream migration of fish. Therefore, we concluded that this fishway owing to be not broad growth width of overflow velocities according to increasing discharges can correspond to variation of water level. Also We consider that various velocities in fishway were effective, because slow velocity line can guide flow for upstream migration. For low flow, the arrangement of different crest level or each overflow part (higher left, middle and lower right, or lower left, middle and higher right) was more effactive than unform crert level. Hole plays an important role as migration pass during drought and flood flow. Therefore, We concluded that this fishway can cope with water depth variation by various overflow wall height change and raise the field applicability with better performance hydraulically and structurally.

PL Study on ZnO Thin Films After H-plasma Treatment (수소 플라즈마 처리를 거친 ZnO 박막에 대한 PL 연구)

  • Cho, Jaewon;Rhee, Seuk Joo
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.28 no.1
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    • pp.17-20
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    • 2015
  • The physical effects of H-plasma treatment on ZnO thin film have been studied using photoluminescence(PL) spectroscopy. Four characteristic peaks have been identified: (i) $D^0X$ peak (neutral donor-bound exciton), showing relatively small integrated intensity after H-plasma treatment, indicates that H-plasma passivates the neutral donors in ZnO at low temperatures. The rapid decrease in the integrated intensity of the peak as the temperature goes up is considered to be due to the ionization of neutral donors. (ii) H-related complex-bound exciton peak appears at the low temperatures (10 K~80 K) after H-plasma treatment, showing the same thermal evolution as $D^0X$ peak. (iii) FX (free exciton) peak starts to show up at 60 K and grows more and more as the temperature goes up, which is considered to be related to the increase in free electron concentration in the film. (iv) violet band is intensified after H-plasma, which means more defects and impurities are generated by H-plasma process.

A study on the effective operation&selection of the BTL project (BTL사업자의 효율적인 선정 및 운영에 관한 연구)

  • Kim, Kyung-Suk;Choi, Moon-Bok;Cho, Young-Ju
    • Proceedings of the Korean Institute Of Construction Engineering and Management
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    • 2007.11a
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    • pp.599-602
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    • 2007
  • To enhance the competition of National power, the investment of SOC is an essential factor for infrastructure construction. The capital for SOC is so gigantic and the national finance is limited, engagement of various subject is necessary. So, Government is promoting the private sector to invest capital in SOC development. Nevertheless the increasement of SOC investment, various problems were issued in selection & operation of BTL. Therefore, to raise the probability of success for BTL project, escalation, unification and specialization of BTL affairs, BTL selection process were suggested in this study.

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A study on the electromagnetic wave absorption properties and microstructure by the composition ratio of Ni-Zn ferrite (Ni-Zn페라이트의 조성비에 따른 전자파 흡수특성과 미세구조에 관한 연구)

  • 조재원;진성빈;문형욱;신용진
    • Electrical & Electronic Materials
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    • v.8 no.6
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    • pp.744-751
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    • 1995
  • This paper describes a study on absorption properties of electromagnetic wave by the composition ratio of Ni-Zn ferrite. Ni-Zn ferrite was composed of 48-52mol% Fe$\_$2/O$\_$3/, 18-22mol% NiO and 26-34mol% ZnO. The sintering of the composition was carried out at 1200.deg. C for 2 hours. Through the experiments, it was found that the resonance phenomenon occured at low frequency range for high permeability and vice versa. In the case of the composition of 49mol% Fe$\_$2/O$\_$3/, 20mol% NiO and 31mol% ZnO, the bandwidth ranged from 0.35GHz to 0.95GHz with the absorption thickness of 10mm. Also, in the case of the composition 51mol% Fe$\_$2/O$\_$3/, 22mol% NiO and 27mol% ZnO, the bandwidth ranged from 0.48GHz to 1.2GHz with the absorption thickness of 6mm.

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The Effect of the ZnO Nanorod Surface on the Optical Property (ZnO 나노막대의 표면이 광학적 특성에 미치는 영향)

  • Cho, Hyun-Min;Rhee, Seuk-Joo;Cho, Jae-Won
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.23 no.2
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    • pp.93-97
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    • 2010
  • We have studied the effect of the chemical composition of the ZnO nanorod surface on the optical characteristics. The surface was treated with H- and O-plasma at different surface temperatures. The chemical composition of the surface of the ZnO nanorod, being investigated by Auger Electron Spectroscopy(AES), was related to the Photoluminescence(PL) data reported in our previous results. The AES showed the opposite results for the $H_2$ and $O_2$ plasma treatments. The ratio of Zn to O on the surface of the ZnO nanorod increased in the case of $H_2$ plasma, while the composition rate of O increased after $O_2$ plasma treatment. The AES results seems to be correlated to the shift in PL peaks. The increase in the composition rate of Zn on the surface of ZnO nanorod is considered to cause the blue shift of the UV peak. On the contrary, the relative increase of O is considered to cause the red shift in PL peaks.

Analysis on the Characteristics of Water Quality in Prearranged Saemangeum Area (새만금 예정수역의 수질특성 분석)

  • Lee, Gwang-Ya;Eom, Myeong-Cheol;Jo, Jae-Won;Jeong, Hae-Jin
    • Journal of The Korean Society of Agricultural Engineers
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    • v.46 no.1
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    • pp.107-117
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    • 2004
  • Hourly monitoring data from Saemangeum estuary, which is expected to become freshwater, was analyzed to evaluate the water quality characteristics. Higher algal growth at spring season than winter influenced the high ratio of organic nitrogen to total nitrogen and concentration of chemical oxygen demand (COD). About 87.9% and 59.7% of organic nitrogen was observed at winter season and spring season, respectively. Daily salinity analysis at the mouth of two main rivers demonstrated that the Dongjin in river was more influenced by tidal effect and showed higher variation than the Mankyung river. The ratio of nitrogen to phosphorus (N/P ratio) was different with site (estuary versus sea area) and season (winter versus spring) remarkably. The N/P ratio was highest (32.74 ∼ 43.93) at estuary in winter and was lowest (1.78 ∼ 3.06) at sea in spring. The high N/P ratio at estuary area implies that phosphorus can be the limiting nutrient factor for algal growth as in general freshwater river, therefore, water quality management practice considering river characteristics rather might be needed in the Saemangeum estuary. The Saemangeum project is nationally recognized for its environmental issues, and especially water quality concern is a critical factor to make policy decision and further assessment with continued monitoring is strongly recommended.