• Journal of Life Science
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• v.27 no.12
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• pp.1437-1444
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• 2017

Sargassum macrocarpum is a widely distributed marine brown algae found in the North Pacific. The objective of this study was to evaluate the anti-inflammatory activity of an ethanol extract of S. macrocarpum (EESM). First, we investigated the anti-inflammatory activities of EESM in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. EESM treatment suppressed nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$) production and inhibited the expressions of the inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) at the mRNA and protein levels. In addition, the expression of pro-inflammatory cytokines, such as tumor necrosis $factor-{\alpha}$ ($TNF-{\alpha}$) and interleukin-1 beta ($IL-1{\beta}$), was decreased in a dose dependent manner. Investigation of the signaling pathways of nuclear factor kappa B ($NF-{\kappa}B$), phosphoinositide-3-kinase (PI3K)/Akt, and mitogen-activated protein kinases (MAPKs) revealed suppression of $NF-{\kappa}B$ translocation from the cytosol to nucleus by EESM treatment. The phosphorylation of the Akt and ERK proteins was also inhibited by EESM treatment. EESM treatment also stimulated the expression of the heme oxygenase-1 (HO-1) enzyme and its upstream transcription factor, nuclear factor-E2-related factor 2 (Nrf2). These results suggest that EESM has anti-inflammatory activity and could have potential uses in the field of nutraceuticals.

• Journal of Environmental Science International
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• v.16 no.8
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• pp.941-957
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• 2007

This study monitored marine environments and ecological characteristics of phytoplankton in southern coastal waters during June to October in 2004-2006 and provided an information to how Cochlodinium blooms have occurred. A total of sampling sites was 16 (Dukyang bay, Goheung, Yeoja bay, Gamak bay, Gwangyang bay, Yeosu, and Namhae). Temperature ranged from $19^{\circ}C\;to\;29^{\circ}C$, which all of sampling in Yeoja bay was the highest temperature of $27^{\circ}C\;and\;29^{\circ}C$ during summer. On June, July, September, and October did not show a remarkable difference regardless of sampling sites. Yeoja and Gwangyang bays had 25-27 in salinity that was lower approximately 5-6 compared with other sampling sites. Chlorophyll had considerable fluctuations depending on sampling sites on July and October, in particular, Gwangyang bay was the highest value of $15{\mu}gl^{-1}$ that had five times as much as. Unlikely to temperature, salinity, and chlorophyll, transparency ranged from 2 m to 5 m regardless of sampling sites. Gwangyang bay was the highest DIN (Dissolved Inorganic Nitrogen) of $0.53mgl^{-1}$ on July and August that had ten times as much as, whereas DIP (Dissolved Inorganic Phosphorus) did not show a significant difference between sampling sites. On July, the correlation of DIN and chlorophyll was a negative that should extremely require DIN to grow phytoplankton, but was a positive liner on August. Mean cell number of phytoplankton reached to encounter a peak of 500 cells $ml^{-1}$ in July and August, which diatoms were dominant species and attained an abundance of >60% regardless of months. In August, the occurrence of dinoflagellates ranged from 20% to 30%. Skeletonema costatum, one of dominant speicies, was the highest occurrence to throughout sampling sites during 2004 to 2006. On the basis of cluster analysis for phytoplankton, they were distributed in all of sampling sites. Consequently, significant fluctuations of marine environments were shown in summer and S. costatum was regarded as the representive phytoplankton in southern coastal waters. In particular, the outbreaks of Cochlodinium polykrikoides have occurred in Dukyang bay, Gamak bay, Goheung, Yeosu, and Namhae, but Yeoja and Gwangyang bays did not occur. The distinguish characteristics of occurring sampling sites and non-occurring in Cochlodinium blooms based on this study was DIN that was enough to persistently grow and maintain them even a litter dissolved in water. This suggests that Cochlodinium red tide seems to be occur in off waters.

• Journal of the Korean earth science society
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• v.28 no.1
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• pp.54-63
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• 2007

The sedimentary sequence of the non-marine Cretaceous Jinju Formation from Sacheon, Korea, contains a number of rod-shaped stromatolites (RSS) characterized by concentric lamination with curd-shaped, stratiform, and small columnar stromatolites. Unlike the world trend, a massive distribution of rod-shaped stomatolite was discovered in the region. The mineral composition, diagenetic alteration, and weathering process of the kind were analyzed by EPMA. The gross morphology of RSS is almost identical to broken plant twigs or stems formed by microbial activity onto which it grew. RSSs are interpreted as stromatolitic algae over plant twigs, which formed through concentric carbonate precipitation by epiphytic algal photosynthesis. The distribution of localities and horizons of the stromatolite imply that RSS is allochthonous and autochthonous. Two types of cyanobacterial filaments and one type of peen algal filament were discovered. The size frequency distribution of calcified filamentous microfossils found in stromatolite was $2.2{\mu}m\;and\;7.8{\mu}m$ in mean diameter of the former, $32.3{\mu}m$ in mean diameter of the latter. The cyanobacterial fossils played a key role in the formation of stromatolite, while the green algal filament was auxiliary stromatolite-builder stromatolites. The filamentous microfossils including trichome were found within the stromatolitic laminae.

• Journal of the Korean Society of Food Science and Nutrition
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• v.39 no.4
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• pp.494-499
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• 2010

In this study, Sargassum coreanum was enzymatically hydrolyzed to prepare water-soluble extracts by using five carbohydrates (Viscozyme, Celluclast, AMG, Termamyl and Ultraflo) and five proteases (Protamex, Kojizyme, Neutrase, Flavozyme and Alcalase) and their potential antioxidant activity were evaluated. The Celluclast and Neutrase extracts of Sargassum coreanum exhibited better DPPH radical scavenging activities (92.42% and 92.78%, respectively) and hydrogen peroxide ($H_2O_2$) scavenging activities (58.28% and 57.97%, respectively) compared to those of other enzymatic extracts. These results suggest that Sargassum coreanum would be a good raw materials for antioxidant and enzymatic hydrolysis would be a good strategy to prepare antioxidant extracts from seaweeds.

• Korean Journal of Environmental Biology
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• v.32 no.4
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• pp.271-285
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• 2014

Aluminum flows into the river from the abandoned mine leachate, industrial wastewater, and sewage and is responsible for acute toxicity in aquatic organisms. Recently, the number of reports have indicated the increased toxicity in a variety of aquatic organisms' due to the aluminum toxicity. In this study, we reviewed the toxicity of aluminum on aquatic invertebrates, fishes and amphibians and suggested the guideline for management of aluminum residues in aquatic environment and strategies for aluminum toxicity evaluation. In aquatic animals aluminum complexes evoke gill dysfunction primarily, the cytotoxicity, genotoxicity, oxidative stress, disruption of endocrine function, reproductive success, metabolism and homeostasis. Notably, at environmentally relevant concentration, aluminum complex can alter the hormone levels in fish in acidic condition. Further, since the solubility of aluminum is higher in the acidic and basic conditions, thus it is likely that the toxic effects of aluminum may not only occur in acidic water near the abandoned mines but also in lakes and rivers, where pH is raised by algal bloom.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.35 no.5
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• pp.476-479
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• 2002

We observed the succession and appearence species of the marine benthic diatoms for the effective seed production of shellfish culture on wave plates in indoor mass culture tanks. Every five days, the existence species and densitiy of diatoms were counted during the 20 culture days. Experimental conditions were 8.5 $\pm$ 0.3$^{\circ}C$ of water temperature, 1.0250$\~$1.0260 of specific gravity, 2,200$\~$13,000 lux of light intensity. The total density of appearence species was rapidly increased with the lapse of time. However, the number of appearence species decreased on the 20th day. The diatoms observed were 10 species; Navicula sp. of, viridula, Cylindrotheca closterium, Licmophora sp., Pleurosisma normnii, Bacillaria Paradoxa Asterionella glacialis, Stephanopuis sp. cf, turris, Entomoneis sp., Odontella aurita and Cocconeis sp, cf, scutellum. Of them, Navicula sp, of, viridula was most dominant throughout the experiment.

• Journal of Life Science
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• v.21 no.1
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• pp.68-73
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• 2011

$\beta$-1,3-glucanase from Pyrococcus furiosus was applied for the saccharification of laminarin, which is a major oligo-saccharide component of brown algae, and the reaction mixture produced from laminarin was utilized as a substrate for alcohol fermentation using yeast. To prepare the recombinant $\beta$-1,3-glucanase, a $\beta$-1,3-glucanase gene was overexpressed in Escherichia coli and purified. Laminarin was degraded to an oligo- and mono-saccharide, such as glucose, after reaction with the purified recombinant $\beta$-1,3-glucanase, and the products after enzymatic treatment were confirmed by TLC and HPLC analysis. Decomposed laminarin after enzyme reaction was only added to the medium as a C-source for yeast alcohol production reaction. 0.3% alcohol production was detected from the cultured broth by gas chromatography after 48 hr of incubation. Further evaluation for optimal conditions of saccharification and alcohol fermentation can be suggested, as well as the possibility of using this enzymatic method to produce ethanol using laminarin.

• Proceedings of the Korea Water Resources Association Conference
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• 2023.05a
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• pp.441-441
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• 2023

통합물관리는 2000년 이전부터 필요성이 논의되다가 2019년 물관리기본법이 제정·시행되면서 수량, 수질, 수생태, 방재 등 분야의 물관리 통합이 단계적으로 추진되고 있다. 수자원장기종합계획, 물환경관리 기본계획, 국가물관리기본계획, 4대강별 유역물관리종합계획 등이 수립되거나 수립중에 있다. 2021년 6월에 수립된 국가물관리기본계획에서는 통합물관리 정량지표들을 물환경, 물이용, 물안전, 물산업, 거버넌스의 5개 분야로 구분하여 제시하였고, 4대강 유역물관리종합계획(안)에서는 국가기본계획의 지표들을 근간으로 유역별 특성을 고려한 정량지표를 설정하고 있다. 수자원장기종합계획부터 유역물관리종합계획까지 통합물관리 정량지표들의 변천 내용과 현황을 검토하였다. 수자원장기종합계획(2016~2020)의 전략과 목표는 "①맑은 물 공급: 급수보급율, 관망 복선화율, 댐 부족량 공급기준, 비상급수 피해인구, 스마트시티 음용률, 누수율 저감, 물 기본법 제정, ②홍수안전 기반구축: 하천기본계획, 하천정비, 수해금액, 도시하천 종합치수대책 수립, 국가하천 홍수예보 지점, 홍수예보시간 단축지점 비율, ③친수환경 조성: 하천유지유량, 하천 이용객, 어류종 및 철새종 증가, ④수자원산업 및 기술개발: 일자리, 수자원산업 육성제도, 해외수주액, 외국 MOU, 국제회의, 남북공유하천 협의"이었다. 물환경관리 기본계획(2016~2025)의 전략과 목표는 "①물순환 체계: 불투수면적률 25% 초과지역, ②깨끗한 물 확보: 상수원 수질달성, ③생태계 서비스 증진: 수생태계 건강성 달성, ④물환경 기반 조성: 산업폐수 유해물질 배출저감, 상수원 4대강 보의 총인 농도와 남조류 세포수, ⑤경제·문화적 가치 창출: 국민체감 만족도"이었다. 국가물관리기본계획에서는 "①물환경: 하천·호소 목표수질 달성률, 수생태계 건강성 B등급 이상 비율, ②물이용: 수돗물 만족률-직·간접 음용률, 노후 상수관로-하수관로 개량, ③물안전: 가뭄피해 인구, 홍수피해 인구-피해액, 댐안전성 강화율, ④물산업: 물관리 R&D 예산, 수량·수질·수생태 통합측정 중권역 비율, 물산업 매출액의 수출액 비중, 한국 주도 국제협력 의제, ⑤거버넌스: 미정"을 계획지표로 하였다. 유역종합계획에서는 4대강별로 거버넌스를 포함하여, "①물환경: 국가계획지표, 주요 상수원 수질, ②물이용: 노후 상하수관로 개량, 지방상수도 유수율, ③물안전: 한해 인구, 수해 인구-피해액, 하천정비율, ④물산업: 물산업 일자리 창출, ⑤거버넌스: 물포럼, 시민참여활동"을 정량지표로 고려하고 있다.

• Korean Journal of Environmental Biology
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• v.32 no.3
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• pp.168-175
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• 2014

In order to understand the phytoplankton community structure based on their cell size duringlow water temperature periods, we studied 10 stations in the East Sea, Korea on March, 2012. The minimum standing crops of total phytoplankton were $3.4{\times}10^6cells\;L^{-1}$ at the station 5. The maximum values were $7.6{\times}10^6cells\;L^{-1}$ at the station 8, which is two times the amount of the minimum. The carbon mass at the station 4 ($6.3{\times}10^8pg\;L^{-1}$) was more than forty times higher compared with station 5 ($0.08{\times}10^8pg\;L^{-1}$). From these results, we found a significant difference between standing crops and carbon mass which might have caused due to their differences in community structure and cell size. Therefore, we considered the types of plankton biomass to estimate the primary product in the specific location and/or time. The phytoplankton communities were classified in 3 types: microplankton (> $20{\mu}m$), nanoplankton (< $20{\mu}m$) and picoplankton (< $2{\mu}m$). In the case of picoplankton, various morphological types were observed during the study period. These various picoplankton species were further classified as S (spherical), SF (spherical&flagella), O (oval), OF (oval&flagella) or R (rod) type, and we analyzed their community structure based on these categories. The picoplankton was found to be the most dominant type at 8 stations and S type as the most popular. The picoplankton seems to be the significant organism in the marine ecology during low water temperature periods in the coastal waters of East Sea. Therefore, picoplankton \;-with scientific surveys can be considered as the database for their identification. In conclusion, we suggest that cell size of the phytoplankton would be the best criteria to accurately analyze their community structure and to reveal groups having more ecological influence.

• Journal of Life Science
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• v.21 no.10
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• pp.1377-1384
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• 2011

Astaxanthin (ATX) is a red-orange carotenoid pigment that occurs naturally in a wide variety of living organisms. In this study we investigated the inhibitory effects of ATX on the induction of inducible nitric oxide synthase (iNOS), nitric oxide (NO), proinflammatory cytokines, nuclear factor-kappa B(NF-${\kappa}B$) and reactive oxygen species (ROS) in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. In addition, we tested the superoxide radical scavenging activity of ATX by scavenging assay. iNOS and NF-${\kappa}B$ expressions were determined by immunoblot analysis. Interleukin (IL)-6 and tumour necrosis factor-${\alpha}$ (TNF-${\alpha}$) were assayed by ELISA. NO production was monitored by measuring the amount of nitrite. ROS was examined by using the 2', 7'-Dichlorodihydrofluorescin diacetate (DCFH-DA) method. At a concentration of 100 ${\mu}M$, ATX inhibited the expression level of LPS-induced NF-${\kappa}B$, as well as the production of LPS-induced NO and proinflammatory cytokines (IL-6 and TNF-${\alpha}$), by suppressing iNOS expression. In particular, the maximal inhibition rate of IL-6 and TNF-${\alpha}$ production by ATX (100 ${\mu}M$) was 65.2----- and 21.2-----, respectively. In addition, ATX inhibited the LPS-induced transcriptional activity of NF-${\kappa}B$, and this was associated with suppressing the translocations of NF-${\kappa}B$ from the cytosol to the nucleus. Moreover, at various concentrations (25-100 ${\mu}M$), ATX inhibited the intracellular level of ROS. At a concentration of 5 mg/ml, the superoxide radical scavenging activity of ATX was 1.33 times higher than ${\alpha}$-tocopherol of the same concentration. These results showed that ATX inhibited the expression of iNOS and the production of NO and proinflammatory cytokines resulting from ROS production and NF-${\kappa}B$ activation in macrophages. Furthermore, ATX was found to be more effective in superoxide radical scavenging activities compared to ${\alpha}$-tocopherol. These findings are expected to strengthen the position of ATX as anti-inflammatory medicine and antioxidant.