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Screening and Identification of the Yeasts for Orange Wine and Their Citric Acid Decomposition (밀감양조주 생산용 효모의 선별, 동정 및 Citric Acid 분해)

  • Ko, Young-Hwan;Kim, Jae-Ha;Koh, Jeong-Sam;Kim, Chang-Jin
    • Korean Journal of Food Science and Technology
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    • v.29 no.3
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    • pp.588-594
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    • 1997
  • Yeast strains useful for the production of wine using mandarine orange, Citrus unshiu, as a main substrate were screened, and their primary ability to decompose citric acid that affects directly wine quality was investigated. Total eleven strains were selected for brewing orange wine. Five wild strains were from soil-based collections and identified: four of them were Saccharomyces cerevisiae and one of them was S. ellipsoideus. The rest of six strains were from among eighteen laboratory strains: three of them were S. cerevisiae, and the other three were S. coreanus, S. uvarum, and S. sake. Two strains of S. cerevisiae out of these selections were chosen and their decomposition of citric acid was investigated. Citric acid was not utilized as sole carbon source for cellular growth. However, when both citric acid and glucose were added together as carbon sources, decrease of citric acid concentration was observed after incubation. Shaking incubation was more effective for the reduction of citric acid than standing incubation. Utilization of citric acid did not contribute to the increase of ethanol concentration during fermentation. On the other hand, it appeared that citric acid caused partial inhibition of cellular growth of the yeasts.

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An Unrecorded Alien Species in Korea: Lactuca saligna L. (Compositae), Melilotus indicus (L.) All. (Leguminosae), Hainardia cylindrica (Willd.) Greuter (Poaceae) (한국 미기록 외래식물: 가는잎가시상추, 좀전동싸리, 나도뿔이삭풀)

  • Ryu, Taebok;Lee, Seungeun;Kim, Namyoung;Lee, JungHyo;Kim, Deokki;Choi, DongHui
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2018.04a
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    • pp.50-50
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    • 2018
  • 본 연구는 국내 생육이 확인된 미기록 외래식물을 보고하고 그 특성을 기술하고자 한다. 가는잎가시상추(Lactuca saligna)는 국화과 다년생 초본이며 높이 10~65cm에 까지 자란다. 줄기에 난 잎은 피침형, 좁은피침형이며, 기부는 이저(耳底), 줄기를 반쯤 감싼다. 유라시아원산으로 지중해, 유럽온대까지 넓은 생육범위를 보인다. 한국에서는 부산, 울산 해안가 주변 공터에서 생육이 확인되었다. 부산, 울산 해안가 저지대를 중심으로 신속한 분포확산이 예상된다. 근연종인 가시상추의 국내 분포확산, 위해성 등을 고려할 때 가는잎가시상추에 대한 주의가 요구된다. 좀전동싸리(Melilotus indicus)는 해안 초지에 생육하는 일년초이며 높이 60cm까지 성장한다. 잎은 우상 3소엽, 소엽은 도란형~좁은도란형, 길이는 1~2.5cm이며 거치가 있다. 좀전동싸리는 국내생육하는 전동싸리와 매우 유사한 형태적 특성을 보인다. 좀전동싸리는 꽃의 길이 2~3mm 개화기는 4~5월이며, 전동싸리는 꽃의 길이 4~7mm 개화기는 7~11월로 차이를 보인다. 지중해, 유럽 남서부 원산으로 전세계적으로 온대 전역에 유입된 것으로 알려져 있다. 국내에서는 제주시 한림읍 옹포리 일대 해안가 저지대 공터 및 초지에서 생육이 확인되었다. 넓은 면적에서 분포하며 제주도에 정착된 것으로 판단된다. 나도뿔이삭풀은(Hainardia cylindrica) 해안 초지에 생육하는 일년초이며 높이 5~35cm까지 성장한다. 수상화서는 25cm에 이르며, 쇠뿔모양으로 단단하다. 소수는 화서 중축에 파인곳을 메우고 있으며, 길이 5~8mm 1개의 소화를 포함한다. 초형은 혁질이며 예두이다. 남아메리카원산으로 북아메리카, 호주 일본 등에 귀화되어 있다. 한국에서는 전라남도 완도군 신지면 신리 해안가 일대 공터 및 초지에 생육이 확인되었다. 나도뿔이삭풀속 Hainardia 은 국내 처음 보고되는 속이며, 뿔이삭풀과 유사한 형태적 특성으로 인해 국명을 신칭하였다.

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Occurrence Pattern and Damage of Scirtothrips dorsalis (Thysanoptera: Thripidae) in Primary Host and Citrus Orchards on Jeju Island (제주도에서 볼록총채벌레의 1차 기주식물 과원과 감귤원에서 발생양상과 피해)

  • Hwang, Rok-Yeun;Kim, Dong-Soon;Hyun, Jae-Wook
    • Korean journal of applied entomology
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    • v.55 no.4
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    • pp.483-487
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    • 2016
  • The damage of citrus fruits caused by Scirtothrips dorsalis Hood has being increased in Jeju, Korea. The seasonal abundance of S. dorsalis and its injury symptoms in citrus orchards have been studied since the endemic outbreak in Jeju, but studies for the occurrence pattern of S. dorsalis in citrus orchards influenced by adjacent primary hosts have not been fully conducted yet. We selected kiwi- and mango-citrus adjacent orchards to study the migration pattern of S. dorsalis toward to citrus orchards. Yellow-colored sticky traps were used for monitoring the seasonal abundance of S. dorsalis and their migration pattern in two orchards. In citrus orchards, also, we placed sticky traps at 0, 5, 10, 15 and 20 m apart from the border of kiwi orchard, and 0, 7 and 20 m apart from the border of mango orchard. The seasonal occurrence pattern of S. dorsalis caught on sticky traps were similar between two adjacent orchards. However, the abundances in kiwi or mango orchard was much higher than those of citrus. Also, it was found that densities, distribution and damage of S. dorsalis in citrus orchards depend highly on the distance from the border of kiwi and mango orchards. This result suggested that the primary hosts such as kiwi and mango plants influence the occurrence of S. dorsalis in adjacent citrus orchards. And this information may provide a basic direction for establishing the management strategy of S. dorsalis in citrus orchards.

Study on the Fish Community in the Seagrass Belt around Cheju Island II. Growth, reproduction and food habit of Tubesnout, Aulichthys japonicus Brevoort (제주도 연안 해초지대의 어류군집에 관한 연구 II. 실비늘치 (Aulichthys japonicus Brevoort)의 성장, 산란 및 식성)

  • Go, You-Bong;Cho, Sung-Hwan;Go, Gyung-Min
    • Korean Journal of Ichthyology
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    • v.9 no.1
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    • pp.61-70
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    • 1997
  • The spawning season, growth and feeding habit of tubesnout, Aulichthys japonicus were examined from the samples collected by a small beam trawl in the Zostera marina belt at the coastal water off Hamduck, Cheju Island, from May 1993 to May 1994. Tubesnout was the dominant species of overall 58 species collected in the study area throughout the year. The larvae and juvenile of tubesnout in the frequency distributions of body length began to appear in the Z. marina belt in March, and adults disappeared after May in the following year. The gonad weight index (GWI) and the condition factor (K) were high from November 1993 to January 1994 for males, while females were high from February to May 1994. Although tubesnout is an oviparous fish, males have the urogenital papilla. These results suggest that they were fertilized between November and January, and then males seemed to be died or move into other places. Females were examined to spawn from February to May. The stomach contents of tubesnout were dominated by copepoda smaller than ca. 1mm, Paracalanus, Oithona, Acartia, Oncaea and Harpacticoidae, accounting for 97% of total food items. The feeding habit of tubesnout did not vary by size. The fishes fed on Calanus, Euchaeta and decapoda larvae larger than ca. 3mm in March and April during the spawning season.

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Composition and Catch Variation of Fishes by a Set Net in the Coastal Waters off Gwideuk, Jeju Island (제주도 귀덕 연안 정치망 어획물의 조성 및 변동)

  • Cha, Byung Yul;Kim, Dae Kwon;Yoon, Jang Taek;Kim, Byung Yeob
    • Korean Journal of Ichthyology
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    • v.20 no.1
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    • pp.28-35
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    • 2008
  • Fishes by a set net in the coastal waters off Gwideuk, Jeju Island were studied to determine species composition and catch variation, 2004. The fishing period conducted by a set net in Gwideuk fishing ground were from April to December. A total of 45,473.2 kg including 21 species was caught during the survey period. Most of them consisted of fishes of 17 species and a few were cephalopods of 4 species. Dominant species were Trachurus japonicus and Siganus fuscescens, 44.3% in the total. And sub-dominant species were Sepioteuthis lessoniana, Seriola dumerili, Seriola quinqueradiata, Loligo bleekeri, Seriola lalandi accounting for 54.4%. Although the number of species had a tendency to decreased from July to December, the catch by a set net was higher in summer and autumn than in spring. Such catch variations were closely related to recruitment of dominant species and also the seasonal variation of fishing ground temperature.

RAPD Analysis and Antimicrobial Susceptibility of Streptococcus equi subsp. zooepidemicus Isolated from Thoroughbred Horses (더러브렛 말에서 분리한 Streptococcus equi subsp. zooepidemicus의 RAPD 분석 및 약제 감수성)

  • Choi, Seong-Kyoon;Park, Yong-Soo;Cho, Kwang-Hyun;Cho, Gil-Jae
    • Journal of Life Science
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    • v.20 no.5
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    • pp.649-654
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    • 2010
  • A total of 68 samples were collected including vaginal mucosa (n=66) from Jangsu stud farm, an equine aborted fetus (n=1), and uterine contents (n=1) from Jeju island. Seventeen Streptococcus equi subsp. zooepidemicus (S. zooepidemicus) strains isolated from horses in Korea were identified as S. zooepidemicus by biochemical tests and sodA.seeI specific multiplex PCR. All isolated strains were divided into 4 clusters: group 1 (No. 2, 3, 5, 6, 7, 11, 12, 13, 14, 15), group 2 (No. 4, 9), group 3 (No. 10, 16, 17), and group 4 (No. 1, 8) by RAPD typing. In group 3, No. 10 isolate that was isolated from vaginal mucosa was indistinguishable from No. 16 and 17 isolates, which were isolated from the equine uterine contents and the equine aborted fetus, respectively. The results of this study suggest that a limited epidemiological relationship exists between the strains from Jangsu (No. 10) and Jeju (No. 16 and No. 17). All isolates showed a high susceptibility to ampicillin, cefoxitin, ceftiofur, cephalothin, florofenicol, gentamicin, nalidixic acid, oxacillin, penicillin, tiamulin, tylosin and vancomycin in antimicrobial susceptibility tests. These results may provide the basic information needed to establish strategies for the treatment and prevention of reproductive diseases in mares in Korea.

Seasonal Variations and Species Composition of Planktonic Ciliates in the Southern Coastal Waters of Jeju Island, Korea (제주도 남부해역의 부유성 섬모충류의 종 조성과 계절 변동)

  • 김요혜;이준백
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.8 no.2
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    • pp.59-69
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    • 2003
  • As part of study on the microbial food web in the southern Jeju Island of Korea, Planktonic tintinnids and aloricate oligotrichs were investigated from July 1998 to June 2000 to understand seasonal variation and water mass indication with environmental factors. 39 species of tintinnids were identified representing 1 order, 11 families, 20 genera. Tintinnid abundance ranged from 100 to 5,400 cells.1$^{-1}$ (mean 314 cells.1$^{-1}$ ), and oceanic species were mainly dominant in fall and winter season, while neritic species were a little pre- dominant in spring and summer season. 15 species of aloricate oligotrichs were identified representing 1 order, 5 families, 7 genera, and many belonged to genus Strombidium which was most abundant and most frequently occurred in many months. Abundance of aloricate oligotrichs ranged from 140 to 21,000 cells.1$^{-1}$ (mean 2,356 cells.1$^{-1}$ ). Species diversity and standing crops of tintinnids were quite different according to seasons, but few seasonal variations were detected in aloricate oligotrichs. In terms of water mass indication tintinnids represented obvious characteristics which were affected by marine environmental factors, but alor- icate oligotrichs had no such apparent indication as tintinnids. Ciliates were more abundant and more diverse in inshore than in offshore station. Total carbon biomass of ciliates ranged from 0.01 to 136.06 $\mu\textrm{g}$C.1$^{-1}$ (mean 5.01 $\mu\textrm{g}$C.1$^{-1}$ ). The carbon biomass, however, did not coincided with seasonal variations of abun- dance Vertical profiles of mean abundance of both tintinnids and aloricate oligotrichs were similar, and had same trend as those of mean chlorophyll o concentration. It suggests that phytoplankton and ciliates reflected the prey-predator relationship in the study area.

Growth Characteristics and Forage Productivity of New Orchardgrass (Dactylis glomerata L.) Variety, 'Onnuri 2ho' (오차드그라스 신품종 '온누리 2호'의 생육특성과 수량성)

  • Ji, Hee Chung;Che, Hyun Seog;Lee, Song Tea;Hwang, Tae Young;Kim, Ki Yong;Lee, Sang Hun
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.36 no.1
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    • pp.15-18
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    • 2016
  • 'Onnuri 2ho' is a new orchardgrass (Dactylis glomerata L.) variety developed by the National Institute of Animal Science (NIAS) in 2014. Four superior clones were selected and polycrossed for seed production to develop this new variety of orchardgrass. The agronomic growth characteristics and forage productivity of 'Onnuri 2ho' were examined at Cheonan from 2010 to 2011. Regional adaptation trials were conducted in Cheonan, Hoengseong, Jinju and Jeju from 2012 to 2014. 'Onnuri 2ho' showed growth habit of medium type in fall and spring. It is long in length of flag leaf with medium upper internode. Plant height of 'Onnuri 2ho' was 4 cm more than that of control variety, 'Amba'. Its heading date was 4 days later than compared to control variety, 'Amba'. Characteristics such as waterlogging and disease resistance of 'Onnuri 2ho' were stronger or better than those of control variety, 'Amba', Especially, the dry matter yield of 'Onnuri 2ho' (15,814 kg/ha) was increased 34 % compared to that of control variety, Amba (11,778 kg/ha). Nutritive feed values of 'Onnuri 2ho' appeared to be similar to that of control variety 'Amba' except in Vitro dry matter digestibility (IVDMD).

Studies on the Mackerel Pures Seine Operating in the Sea Area of Cheju Island - 4 . The Characteristics of Catch and Ability in Purse Seine - (제주도 주변해역 고등어 건착망의 연구 - 4 . 어획량의 특성과 어구의 성능 -)

  • 박정식
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.27 no.4
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    • pp.247-254
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    • 1991
  • Mackerel purse seine fishery is one of the most important fisheries in Korea and its annual mean catch from 1982 to 1988 is 350 thousand tons. Between 1982 and 1988, the characteristics of catch at 5 sectors of the sea area was analyzed according to operating sea block and the catch by fishes based on the data obtained from daily report of fishing condition that had been made out by 48 Korean fishing boats engaged in the fishery of purse seine. In these 5 sectors of the sea area, catch per net hauling in the coastal area of Cheju Island is less than that in the other sectors. Accordingly, the shape of fishing gear during operation has been measured to analyzed the deformation of net shape caused by the bottom current. (The lunar calender is used in the paper) The results obtained are as follows; The average CPUE of purse seines is 31.6 tons and it is higher from October to April than that from May to September. In the 5 purse seine fishing grounds, the ratio of net haul in the coastal sea area of cheju Island is 42.4% and it is the highest level, but the CPUE is 25.7 tons which is relatively low level. Each CPUE in the sea areas is significant differences with 1% level. Index of seasonal variation of mackerel CPUE from May to October is lower than the standard value, whereas index from November to January is higher. The diameter of circle by net shooting becomes shorter to the minimum caused by the bottom current during loose set, becomes narrower and the efficiency of fish encirclement becomes lower. The shape of leadline by bottom current becomes long oval type along with current direction in the tight set, while it becomes long oval type in the direction of a right angle to the current direction and the enclosed area is reduced hurriedly, and the deformation of net is most serious in the loose set.

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Effect of Planting Distance on the Growth and Yield of Brussels Sprouts in the Highland Area (방울다다기양배추 고랭지 재배시 재식거리가 생육 및 수량에 미치는 영향)

  • Um, Nam Yong;Kim, Ki Sun;Lee, Jae Hee;Jun, Shin Jae;Lee, Won Gung;Kim, Gung Won
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.10a
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    • pp.53-53
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    • 2019
  • 방울다다기양배추는 아직까지 국내에서는 소비자들에게 인지도가 낮은 작물이지만 최근 식생활 및 소비 패턴이 변화하면서 편의성, 차별성 등이 중요시면서 미니 과일, 미니채소류에 대한 관심이 증가하고 있다. 또한 외국의 도입 채소류에 대한 관심이 증가하면서 2013년 방울다다기양배추 수입량이 36톤이었던 것이 2015년에는 14.3배가 증가한 515톤으로 증가하였다. 이를 통해 방울다다기양배추가 가지고 있는 기능성과 미니채소에 대한 관심으로 지속적으로 소비가 증가할것으로 예상되고 있다. 이와같이 증가추세에 있는 방울다다기양배추의 수입을 대체하기 위해서는 국내 재배면적 확대가 필요하나 저온성 작물인 방울다다기양배추는 고온기 남부지역에서는 재배가 어려워 제주지역의 겨울재배 작형으로 제한되어 재배가 되고 있다. 따라서 제주지역의 겨울재배 작형과 연계하여 강원도 고랭지의 서늘한 여름 기후를 이용하여 단경기인 9~11월에 생산할 수 있는 여름재배 작형을 개발할 필요성이 있어 시험을 수행하였다. 본 시험은 2018년~2019년 태백 철암의 노지(표고 750 m)에서 시험품종은 "브릴리언트"를 이용하여 3월 하순 파종하여 50일 육묘하여 5월 10일 포장에 정식하였다. 흑색멀칭한 시험포장에 $80{\times}30cm$부터 $80{\times}60cm$까지 10 cm 간격으로 차이를 두고 정식하였으며 정식 전 N-P-K = 20-15-20 kg/10 a를 시비하였고 시험포장은 수확까지 병해충 방제 등 주기적으로 관리하면서 생육특성 및 수량성을 조사하였다. 재식거리별 생육상황을 비교한 결과 초장은 처리간 큰 차이가 없었고 경경은 재식거리가 커질수록 증가하는 경향이었다. 또한 엽장 및 엽폭은 처리간의 경향이 없었으며 엽수는 재식거리가 커질수록 증가하였고 $80{\times}30cm$ 처리시 80매내외였던 것이 $80{\times}50cm$ 이상일 경우 100매 이상으로 증가하였다. 재식거리별 구의 특성을 조사한 결과 구경은 처리간 큰 차이는 없었으나 재식거리가 커질수록 구경이 다소 증가하였으며 구중도 증가하는 경향이었다. 방울다다기양배추의 구의 엽수는 처리간 큰 차이가 없었고 경도 및 당도도 큰 차이가 없었다. 처리별 수량은 $80{\times}40cm$$80{\times}50cm$ 처리에서 수량이 다소 증가하였고 상품화율은 $80{\times}50{\sim}60cm$ 처리에서 80%이상으로 높았다. 따라서 방울다다기양배추의 여름 고랭지 재배시 재식거리는 $80{\times}50cm$ 처리시 생육 및 엽수 증가에 따른 구의 형성이 증가하여 수량성이 높아지고 상품화율도 증가하였다.

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