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Differentiation of Thyroid Gland and Changes of Thyroid Hormone Concentration during Early Development of Black Rockfish, Sebastes schlegeli (조피볼락, Sebastes schlegeli 자치어의 갑상선 분화 및 갑상선호르몬의 변화)

  • Kang, Duck-Young;Chung, Ee-Yung;Chang, Young-Jin
    • Development and Reproduction
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    • v.5 no.1
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    • pp.39-45
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    • 2001
  • Black rockfish Sebastes schlegeli is one of scopaenid fish giving birth to yolk-sac larvae and distributed around the coast of Korea and Japan, and is one of main species which is cultured in Korea. The difffrentiation of thyroid gland and the changes of thyroid hormones (THs) concentrations in the whole body during early development of this species were examined and the relationship between thyroid gland and their growth will be presented. Total length (TL) and body weight (BW) of the larva at the parturition were 6.3${\pm}$0.1 mm and 3.6${\pm}$0.1 mg, respectively. The larvae transformed to juvenile after 30th day after parturition. According to Hoshiai(1977), they had gown into stage Fl (TL 13.2${\pm}$0.9 mm, BW 46.5${\pm}$1.5 mg) at 50th day. The thyroid gland of black rockfish was first observed histologically in hatching larvae in mother fish. The larvae just after parturition have 1${\sim}$3 of the thyroid follicles diffrentiated between basibranchial bone and ventral aorta, at the base of the first gill arch. In this time, thyroid follic1e number (TFN),thyroid follicle diameter (TFD) and thyroid cell height (TCH) were 1.6${\pm}$0.8 pieces/inds., 18.1${\pm}$0.6 ${\mu}$m and 4.1${\pm}$0.2 ${\mu}$m, respectively. TFN and TFD at 50th day were increased to 32.5${\pm}$6.9 pieces/inds. and 41.5${\pm}$1.7 ${\mu}$m, respectively. These results indicate that they are related to the growth of black rockfish during early development. However, TCH indicates that the activity of thyroid gland appearedat special day, eg. 5, 20 and 50th day, suggesting that TCH may have some role in the physiological activity. L-thyroxine (T$_4$)concentration decreased sharply to 10 days after parturition, and at 25th day (metamorphosis stage) increased markedly to 3.44${\pm}$0.93ng/g fish. After this time, T$_4$ concentration decreased at 35th day, but then increasedagain to the highest concentration, 5.63${\pm}$0.70ng/g fish. 3,5,3'-triiodo-L-thyronine (T$_3$) concentration declined sharply from just after pafurition (4.96${\pm}$1.90 ng/g fish) to 5th day (0.30${\pm}$0.07 ng/g fish). However T$_3$ concentration increased markedly to 0.95${\pm}$0.11 ng/g fish at 30th day and then did not significantly change until 45th day, increased also sharply to 1.67${\pm}$0.23 ng/g fish at 50th day.

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A study on measures for the mitigation of fire damage in Korea super high-rise building through the improvement of domestic·foreign standards (국·내외 기준개선을 통한 국내 초고층 건축물의 화재피해경감 대책에 관한 연구)

  • Ko, Jaesun
    • Journal of the Society of Disaster Information
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    • v.13 no.2
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    • pp.233-248
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    • 2017
  • Uniform laws and regulations and reasonable design is necessary for the prevention of possible fire in super high-rise building. To this end, this study focused on super high-rise and massive building-related architectural review performance-based design (PBD) evaluation disaster impact assessment (DIA), and provided fire engineering measures for improving fire prevention on the basis of performance-based design by analyzing the buildings subject to these systems and problems in terms of contents. Above all, in the aspect of law and standard improvement, first, with regard to dual parts of two statutes though significant portion of them has the same contents in performance-based design (PBD) evaluation and disaster impact assessment (DIA), it is necessary to operate the systems after making them conform with each other and consolidating or abolishing them. Second, if it is impossible to consolidate or abolish performance-based design (PBD) evaluation and disaster impact assessment (DIA), the areas of contents of performance-based design (PBD) evaluation and disaster impact assessment (DIA) should be precisely classified and established. Next, engineering improvement measures against fire hazard in super high-rise building are as follows. First, it is necessary to revise the provisions of straight-run stairs in special escape stairs. And in case of installing a mechanical smoke exhaust system instead of smoke vent, sandwich pressurization used in the United Stated should be permitted. Second, with regard to smoke control system for special escape stairs, it was shown that there was necessity for revising the standards in order to enable air to be supplied according to section in case of fire, carrying out performance-based design, and the like from the early design stages to the completion stages. In the future, it is expected that an epoch-making contribution will be made to a decrease in casualties and property damage due to fire in case of super high-rise building where the results can be reflected after carrying out a study on maintenance and carrying out an additional study on other considerations of super high-rise building together with reflecting the improvement measures provided in the above-mentioned study.

Recovery of N and P Resources from Animal Wastewater by Struvite Crystallization (Struvite 결정화에 의한 축산폐수로 부터 질소.인 자원의 재생)

  • Jo, W.S.;Yoon, S.J.;Ra, C.S.
    • Journal of Animal Science and Technology
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    • v.45 no.5
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    • pp.875-884
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    • 2003
  • Operational parameters for struvite crystallization, as a process to recover nitrogen and phosphorus resources from animal wastewater, were studied in this research. Crystallization distinctive of NH$_4$-N and PO$_4$$^{3-}$ in accordance to chemical sources, influent pH, aeration and stirring was examined using 2L of working volume of struvite reactor. Also, to find an effective treatment process combining with electrolysis method, removal characteristics of NH$_4$-N and PO$_4$$^{3-}$ in 6 different processes was tested. As chemical sources for the derivation of struvite formation, MgSO$_4$ and MgCl$_2$ were superior to CaCO$_3$ and CaCl$_2$. From experiment which was conducted to know the effects of aeration and stirring on struvite formation, it was revealed that aeration stimulated the crystallization reaction by inducing faster pH increase. While 90% of P removal was achieved within 1 hour under aeration, 14 hours was consumed under stirring condition. Struvite formation under aeration was affected by influent pH. No crystallization was observed at pH 5 level, but active crystallization reaction was induced over pH 6.0. 95% of P removal by struvite formation at pH 6, 7 and 9 was achieved within 3h, 2h and 10 min., respectively. However, over pH 10, operational problem due to excessive foam formation occurred, and blunting of crystallization reaction was observed at pH 11. When consider the pH range of animal wastewater, pH 7 to 9, efficient struvite formation could be achieved by simple aeration, without any chemical usage for pH adjustment. Among tested processes, the treatment process which electrolyzing the supernatant from struvite reactor, providing air to both reactors, showed best pollutant removal efficiencies. In this combined process, the removal efficiencies of NH$_4$-N and PO$_4$$^{3-}$ was 86% and 98%, respectively, and 92.4% of color removal was obtained.

Effects of Increasing Market Weight of Finishing Pigs on Backfat Thickness, Incidence of the 'Caky-fatty' Belly, Carcass Grade, and Carcass Quality Traits (비육돈의 출하체중 증가가 등지방두께, '떡지방' 삼겹살 발생률, 도체등급 및 도체의 품질 특성에 미치는 영향)

  • Park, Man Jong;Park, Byung Chul;Ha, Duck-Min;Kim, Jin-Bo;Jang, Kyoung-Soon;Lee, Do-Hyun;Kim, Gwan-Tae;Jin, Sang-Keun;Lee, C. Young
    • Journal of Animal Science and Technology
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    • v.55 no.3
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    • pp.195-202
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    • 2013
  • The present study was undertaken to investigate the effects of increasing pig market weight on the incidence of the 'caky-fatty' belly, carcass grade and carcass quality traits. To this end, 500 (Yorkshire ${\times}$ Landrace) ${\times}$ Duroc market pigs consisting of equal numbers of barrows and gilts were slaughtered at body weights ranging from 85 to 150 kg and their carcasses were analyzed. Backfat thickness (BFT; mm) of the barrows and gilts regressed on live weight (kg) linearly as follows: BFT (y) = 0.1827x + 3.4825 ($r^2$ = 0.4671) and y = 0.2015x-0.6972 ($r^2$ = 0.4736), respectively. The caky-fatty belly, which, by definition of the present study, had = 55% fat by weight in the dorsal-side half of a 5 cm-wide belly strip between the $11^{th}$ and $12^{th}$ ribs, was found in 10 carcasses from barrows. However, no belly was condemned as 'caky-fatty' by the retailers. Total number of carcasses which were down-graded on account of overweight was as many as 101 (20.2%). The $1^+A$-grade carcasses exhibited a superior intramuscular fat score to all other grades, but except for this and the defective quality traits, no visible differences were found in carcass quality among different carcass grades. In conclusion, the current carcass grading system, which is sparingly reflective of the carcass quality and also under-evaluates the larger but otherwise normal carcasses, may as well be revised. Moreover, the incidence of the caky-fatty belly appears not to be a critical factor to be considered when increasing the pig market weight.

Uptake and Translocation of Heavy Metals to Rice Plant on Paddy Soils in "Top-Rice" Cultivation Areas (탑라이스 생산지역 논 토양 중 잔류중금속의 벼 흡수이행)

  • Park, Sang-Won;Yang, Ju-Seok;Ryu, Seung-Won;Kim, Dae-Yeon;Shin, Joung-Du;Kim, Won-Il;Choi, Ju-Hyeon;Kim, Sun-Lim;Saint, Andrew Flynn
    • Korean Journal of Environmental Agriculture
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    • v.28 no.2
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    • pp.131-138
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    • 2009
  • Heavy metal residues in soil, rice straw, unhulled rice, rice hull, polished rice, and rice barn on the rice paddy in the "Top rice production complex which is non-contaminated area were evaluated. It was observed that the average concentrations of As, Cd, Cu, Pb, and Hg in the paddy soils were 1.235, 0.094, 4.412, 4.728 and 0.0279 mg/kg, respectively. There were no cultivation areas exceeded of the threshold for soil contamination designated by "The Soil Environment Conservation Law" in Korea. For the polished rice, there were no samples exceeded of a permissible level of heavy metal residues such as 0.051 mg/kg of As, 0.040 mg/kg of Cd, 0.345 mg/kg of Cu, 0.065 mg/kg of Pb and 0.0015 mg/kg of Hg. For the uptake and translocation of heavy metals to rice plant, a main part of heavy metal accumulation was rice straw, and then rice bran. Furthermore, it shown that accumulation of heavy metals in unhulled rice, rice hulls, brown rice, and polished rice was approximately similar as low. The slopes of translocation of heavy metals from soil to polished rice were following order as Cd, 0.4321 > Cu, 0.054 ${\fallingdotseq}$ Hg, 0.052 > As, 0.021 > Pb, 0.008. It was observed that potential ability of Cd uptake in rice plant and then its translocation into polished rice was very high. Concentrations of copper and mercury absorbed in the rice plant were moderate for translocating into the polished rice, while the arsenic and lead in the plant were scarcely translocated into the polished rice. The distribution of heavy metals absorbed and translocated into aboveground parts of rice plant was appeared that there were remained at 63.3-93.4% in rice straw, 6.6-36.9% in unhulled rice, 0.6-5.7% in rice hulls, 3.2-31.3% in brown rice, 0.8-4.6% in rice bran and 1.1-26.7% in polished rice. The accumulation ratio of Cd in the aboveground parts of rice plant was remained at 26.7-31.3% in brown and polished rice.

The Comparison between High Dose and Low Dose Morphine in Terminal Cancer Patients During the Last 1 Week to Death (고용량과 저용량의 몰핀을 쓰는 말기 암 환자에서 임종 1주일동안 비교)

  • Cho, Doo-Yeoun;Cha, Kyu-Jin;Yoon, Bang-Boo;Yeom, Chang-Hwan
    • Journal of Hospice and Palliative Care
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    • v.5 no.1
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    • pp.24-30
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    • 2002
  • Background : Pain is one of the most feared consequences of cancer. $65{\sim}85%$ of cancer patients experienced severe pain, and sometimes high dose morphine is used to these patients. But many doctors still have 'opioid-phobia' and hesitate to use high dose morphine. We investigated the morphine therapy in terminal cancer patients during the last 1 week to death, and found any differences according to the morphine dosage. Methods : 93 patients admitted to National Health Insurance Corporation Ilsan Hospital, department of family medicine for hospice care between September 2000 and the end of October 2001 and lived more than 1 week entered in the study. We investigated the demographic data, laboratory tests and sufficient dosage of morphine for pain control. According to the calculated dosage by OME(oral morphine equivalent), patients were divided into low dosage group (${\leq}150mg/day$) and high dosage group (>150 mg/day). The chi-squared test were used to evaluate the influence of age, gender, tumor sites, metastasis and adverse effects of morphine. Results : Mean age was $65.0{\pm}13.1year$ in low dosage group and $59.9{\pm}11.6year$ in high dosage group. 32 men (50.0%) and 32 women (50.0%) were included in low dosage group and 15 men (51.7%) and 14 women (48.3%) in high dosage group. Stomach was the most frequent tumor site and lung was the next. Metastasis were found 58 (90.6%) in low dosage group and 28 (96.6%) in high dosage group. In other palliative radiotherapy and adverse effects, there were no differences in both group. Conclusion : During the last 1 week to death in cancer patients, there were no difference according to the morphine dosage. So we don't have to have 'opioid-phobia' in treating the terminal cancer patients.

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Effect of Long-Term Steeping and Enzyme Treatment of Glutinous Rice on Yukwa Characteristics - II. Physicochemical Characteristics of Enzyme-treated Glutinous Rice Flour - (찹쌀의 장기 수침 및 효소처리가 유과의 특성에 미치는 영향 -제 2 보: 효소처리시킨 찹쌀가루의 이화학적 특성 연구-)

  • Sohn, Kyung-Hee;Park, Jun
    • Korean journal of food and cookery science
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    • v.14 no.3
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    • pp.225-231
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    • 1998
  • Enzyme-treated glutinous rice flour, which was developed to shorten or skip a steeping process during the preparation of Yukwa, was analyzed for its physicochemical characteristics and compared with glutinous rice flour made by 28-day-steeping method. Total sugar content of the 28-day-steeped flour was the highest among all groups, on the other hand, the reducing sugar content was higher in enzyme-treated glutinous rice flour. The viscosity of enzyme-treated flours was significantly lower than that of the 28-day-steeped and particularly showed the lowest value at 65$^{\circ}C$. The contents of Ca$\^$2+/ and Mg$\^$2+/ in enzyme-treated glutinous rice flours were higher than those of the 28-day-steeped group, however, the content of P$\^$+/ was lower. Free sugar detected in glutinous rice flour prepared from 28-day-steeping method was glucose only, but enzyme-treated flours contained maltose and glucose, and the content of total free sugar was much higher than that of the 28-day-steeped group. In microscopic structure, both 28-day-steeped and enzyme-treated flours showed the particle size decreased and porous surface on some part of the flour granule.

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Questionnaire Study on the Difficulties and Improvements of the 6th Industrialization Dairy Farm (설문을 통한 6차산업형 목장경영의 애로사항과 개선방안에 관한 연구)

  • Lee, Jin-Sung;Nam, Ki-Taeg;Park, Seong-Min;Son, Yong-Suk
    • Journal of Dairy Science and Biotechnology
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    • v.34 no.4
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    • pp.255-262
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    • 2016
  • This study was conducted to investigate the difficulties of dairy farms in practicing 6th industrialization and methods for overcoming these difficulties. A qustionnaire survey was carried out to examine the present states of farms, recognition of the farmstead milk-processing market situation, possibility of farmstead milk processing for reducing the raw milk surplus, assessment of government policies, and difficulties dairy farmers confront in realizing the 6th industrialization. Farm sizes, types, and human resources organizations varied between farms. Most farmers were producing yogurt and/or fresh (string or barbecue) cheeses, which were marketed through 'Visit and Purchase' channel. Farmers who answered the questionnaire were relatively positive about the current situation of farmstead milk processing, expecting to be involved in the disposal of excess raw milk. Nevertheless, they responded negatively about current relevant policies, citing the main difficulties caused by 'excessive regulation'. Other barriers to successful '6th industrialization' are difficulties in marketing and lack of funds. Approximately 19% of dairy farms practicing the '6th industrialization' use automatic milking system (AMS) and 38.46% of dairy farmers whose milking depends on conventional milking system intend to introduce AMS in the future. Positive expectations of AMS adoption were mostly related to 'lack of time and labor', 'exhibiting for tourism', and 'succession of dairying'.

Population Structure and Fine-scale Habitat Affinity of Cymbidium kanran Protected Area as a Natural Monument (천연기념물 한란 보호구역의 개체군 구조 및 미세 서식처 선호성)

  • Shin, Jae-Kwon;Koo, Bon-Youl;Kim, Han-Gyeoul;Kwon, He-Jin;Son, Sung-Won;Lee, Jong-Seok;Cho, Hyun-Je;Bae, Kwan-Ho;Cho, Young-Chan
    • Korean Journal of Ecology and Environment
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    • v.47 no.3
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    • pp.176-185
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    • 2014
  • There are no population ecological research on the natural monument (No. 191) Jeju Cymbidium kanran in South Korea. In this study, we analyzed the population structure and fine-scale habitat affinity of C. kanran in Sanghyo-dong, Jejudo Island from Oct. 2013 to Feb. 2014. We observed total of 1,237 individuals (4,341 pseudobulbs) of C. kanran (989.6 population $ha^{-1}$) within (1.25 ha) and only 17 (1.4%) individuals were inflorescent. In 60.9% of the entire populations, disease symptoms such as spots and blight leaves were observed. C. kanran populaton exhibited reverse-J shaped size distribution based on leaf area classes as individual size parameter. The three size related attributes of C. kanran (no. of pseudobulb $r_s$=-0.159, no. of leaves $r_s$=-0.148 and leaf arera $r_s$=-0.114) and soil temperature revealed a negative relationship (p<0.0001). Most of C. kanran (95.4%) were grown under Castamopsis cuspidata and spatially, C. kanran were strongly clumped at all distances. Population characteristics of C. kanran in the study area were likely originated from species habitat affinity and successional environment. Through this study, base line data for C. kanran's habitat monitoring was established and conservation measures based on population characteristics were discussed.

Ethanol Extracts of Mori Folium Inhibit Adipogenesis Through Activation of AMPK Signaling Pathway in 3T3-L1 Preadipocytes (3T3-L1 세포에서 상엽이 유발하는 AMPK signaling pathway를 통한 adipogenesis 억제에 관한 연구)

  • Ji, Seon Young;Jeon, Keong Yoon;Jeong, Jin Woo;Hong, Su Hyun;Huh, Man Kyu;Choi, Yung Hyun;Park, Cheol
    • Journal of Life Science
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    • v.27 no.2
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    • pp.155-163
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    • 2017
  • Mori Folium, the leaf of Morus alba, is a traditional medicinal herb that shows various pharmacological activities such as antiinflammatory, antidiabetic, antimelanogenesis, antioxidant, antibacterial, antiallergic, and immunomodulatory activities. However, the mechanisms of their inhibitory effects on adipocyte differentiation and adipogenesis remain poorly understood. In the present study, we investigated the inhibition of adipocyte differentiation and adipogenesis by ethanol extracts of Mori Folium (EEMF) in 3T3-L1 preadipocytes. Treatment with EEMF suppressed the terminal differentiation of 3T3-L1 preadipocytes in a dose-dependent manner, as confirmed by a decrease in the lipid droplet number and lipid content through Oil Red O staining. EEMF significantly reduced the accumulation of cellular triglyceride, which is associated with a significant inhibition of pro-adipogenic transcription factors, including sterol regulatory element-binding protein-1c (SREBP-1c), peroxisome proliferator-activated receptor-${\gamma}$ ($PPAR{\gamma}$), and CCAAT/enhancer-binding proteins ${\alpha}$ ($C/EBP{\alpha}$) and ${\beta}$ ($C/EBP{\beta}$). In addition, EEMF potentially downregulated the expression of adipocyte-specific genes, including adipocyte fatty acid binding protein (aP2) and leptin. Furthermore, EEMF treatment effectively increased the phosphorylation of the AMP-activated protein kinase (AMPK) and acetyl CoA carboxylase (ACC); however, treatment with a potent inhibitor of AMPK, compound C, significantly restored the EEMF-induced inhibition of pro-adipogenic transcription factors and adipocyte-specific genes. These results together indicate that EEMF has preeminent effects on the inhibition of adipogenesis through the AMPK signaling pathway, and further studies will be needed to identify the active compounds in Mori Folium.