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Effects of Transforming Growth Factor $\beta$ on In-vitro Maturation of Porcine Oocytes (Transforming Growth Factor $\beta$가 돼지 난자의 체외성숙에 미치는 영향)

  • 신명균;박춘근;조재원;정희태;양부근;김정익
    • Korean Journal of Veterinary Service
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    • v.21 no.3
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    • pp.267-275
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    • 1998
  • 돼지 수정란의 체외생산은 난자의 체외성숙과 체외수정에 관한 기술의 부족으로 아직까지 만족스럽지 못한 수준이다. 특히 돼지 수정란의 체외생산에는 복잡한 세포질의 성숙과정과 높은 다정자침입율 및 전핵형성의 억제등의 문제점이 있다. 본 연구에서는 돼지 난자의 체외성숙 체계를 개선하기 위하여 transforming growth factor$\beta$(TGF$\beta$)의 첨가가 난자 및 난구세포에 미치는 효과에 대하여 검토하였다. 체외성숙용 배지에 TGF$\beta$를 1~10ng/$m\ell$의 농도로 첨가하여 미성숙 난자를 배양한 결과 성숙율이 높아졌다. TGF$\beta$의 효과는 난구세포가 제거된 난자의 성숙에도 효과적이었다. TGF$\beta$(를 첨가하지 않은 배양액 내에서는 배양 24시간 까지 metaphase-II로 성숙된 난자가 관찰되지 않았으나 TGF$\beta$를 첨가한 배양액 내에서는 관찰되었다. 한편, 난구세포가 부착된 난자의 성숙배양시 TGF$\beta$의 첨가시기에 따른 차이는 없었으나, 난구세포를 제거한 난자의 경우에는 성숙배양 전반기(59%) 또는 후반기(57%) 24시간 동안에만 TGF$\beta$를 첨가하는 것이 48시간 동안 계속하여 첨가(27%)하는 경우 또는 비첨가(38%)에 비하여 유의적으로 높은 성숙율을 나타냈다(p<0.05). 이와 같은 결과는 난구 세포가 돼지 난자의 체외성숙에 필수적이지만 TGF$\beta$는 난구세포가 제거된 난자의 체외성숙에 어 느정도 유익한 효과를 발휘하는 것으로 추측된다.

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Cracks Information Analysis of Concrete Slab Surface (콘크리트 슬래브 표면의 균열 정보 분석)

  • No, Dae-Kyeung;Kim, Kwang-Beak
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2008.10a
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    • pp.399-405
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    • 2008
  • 콘크리트 슬래브 표면의 영상처리 목적은 구조물의 균열발생을 최소화하고 발생한 균열에 대해서 지속적인 기록 관리로 균열의 진행 상태를 분석하여 보수시기 및 보수공법 적용의 합리적 운영체계를 수립하고자 하는 것이다. 일반적으로 콘크리트에서의 균열은 육안으로 분간할 수 있을 정도로 큰 반면 프리스트레스트 콘크리트의 균열은 기기를 사용하여야 측정, 분별할 수 있다. 본 논문에서는 균열의 명함도와 인접한 표면의 명함도 차이를 이용하여 균열과 잡음을 분리한다. 그리고 균열과 잡음 영역에서 형태학적인 정보를 이용하여 잡음 영역만을 제거한다. 잡음 영역이 제거된 균열 영역에서도 미세한 잡음이 존재하므로 균열 영역을 평활화를 한 후, 미세 잡음을 하나의 객체로 인식하여 제거하고 끊어진 영역의 균열을 미디언 필터를 이용하여 균열을 연결한다. 그리고 제안된 방법으로 추출된 균열을 원본 영상에 적용하여 최종적으로 균열만을 검출한다. 검출된 균열에서 균열의 특정부분을 선택하고 선택되어진 균열 영상을 대상으로 균열의 길이, 방향, 폭을 순차적으로 분석한다. 실제 균열 영상을 대상으로 실험한 결과, 균열이 비교적 정확히 검출되었고 균열 검사기가 한번에 측정할 때에 비해 비교적 빠르고 적은 노력으로 측정할 수 있는 것을 확인하였다.

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Seasonal Occurrence and Infection Site of Strawberry Anthracnose (딸기탄저병의 시기별 발생과 감염경로)

  • 김승한;최성용;임양숙;윤재탁;최부술
    • Research in Plant Disease
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    • v.8 no.1
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    • pp.45-49
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    • 2002
  • Seasonal occurrence of strawberry anthracnose in greenhouses caused by Colletotrichum sp. was examined from 1997 through 1999 at three locations, Kyeongju, Goryeong, and Cheongdo in Kyungbuk province, Korea. Also some factors related to the anthracnose infection such as initial infection sites, inoculation methods, and soil nature were studied through in vitro and field experiments. The anthracnose disease begun to occur from 15 days after transplanting in early October, and continued but gradually decreased thereafter for 2 months until December, After transplanting, initial infection mainly occurred through the runner of which the tissue was more susceptible to the anthracnose than those of the leaf and petiole when the fungal mycelial disk was inoculated. Postplanting inoculation by irrigation with spore suspension was much more effective in inducing the anthracnose disease than preplanting soil mix. However without inoculation, no or little anthracnose occurred regardless of commercial, non-cultivated or diseased field soils when healthy seedlings were planted. This suggests that occurrence of strawberry anthracnose in fields may be related to contamination of plant propagules with the anthracnose pathogen.

Environmental-Friendly Control of the Ginseng Stem Fungus Gnat (Phytosciara procera, Diptera) in the Ginseng Field (인삼재배지에서 인삼줄기버섯파리(Phytosciara procera)의 친환경 방제)

  • Seo, Mi-Ja;Shin, Hyo-Seob;Gawk, Chang-Soon;Jo, Shin-Hyuk;Cho, Hye-Sun;Shin, Jung-Sup;Cho, Dae-Hui;Youn, Young-Nam;Yu, Yong-Man
    • The Korean Journal of Pesticide Science
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    • v.15 no.4
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    • pp.471-478
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    • 2011
  • The ginseng stem fungus gnat, Phytosciara procera, overwintered in old stems and roots of ginseng as a larvae from late September to early May in next year. In the late Spring they become pupa then emerged. Therefore, the first control period has to be set between late May and early June. It is a same period of elimination of ginseng flower stalks. Two or three times pest control might be needed per month for controlling adult of P. procera. Otherwise, the overwintered form as larva in old stem by removal of old stems of ginseng, the population of first generation of P. procera adults could be reduced. However, it seemed that the removal of old stems was not necessarily correlated with the population of P. procera adults in the next generation. Three kinds of environmental-friendly control agents that were mainly contained sophora extract, showed the highly insecticidal activities against larva of P. procera. Among them, one agent containing sophora extract and paraffin oil showed an ovicidal action as well as oviposition repellency.

Effect of Apex Removal at Different Leaf Ages on the Growth and Yield of Broccoli. (엽령이 다른시기의 생장점 제거가 브로콜리의 생육 및 수량에 미치는 영향)

  • Seong, Ki-Cheol;Lee, Jae-Wook;Kwon, Hyeog-Mo;Moon, Doo-Yong;Kim, Chun-Hwan
    • Journal of Bio-Environment Control
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    • v.13 no.1
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    • pp.21-25
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    • 2004
  • This experiment was carried out to investigate the effect of apex removal at different leaf ages on the growth and yield of broccoli in pinching cultivation. Apex was pinched when 2, 3 or 4 true leaves were spread during nursery bed, and non-pinching was conventional method. The plant height was not different among treatments but the fresh weight was decreased as the pinching days were delayed. The numbers of leaves and lateral shoots were increased compared to those of non-pinching by pinching. Days to central budding were shortended by 3 days when pinched at 2 true leaves spreading stage than those of non-pinching. Central (1st) head weight was heaviest when pinched at the time of 2 true leaves spreading (157g). Total head weight was heaviest when pinched at the time of 2 true leaves spreading (378g), and lowest at 4 true leaves spreading stage (196g). Marketable yields were increased by 48% when pinched at the time of 2 true leaves spreading (1,754kg) as compared with those of non-pinching (1,184kg) and lowest when pinched at the time of 4 true leaves spreading (820kg). Also, first harvest was shortended by 6 days by pinching at 2 true leaves spreading stage.

Effects of Apex Removal on the Growth and Yield of Artichoke by Planting Times (정식시기별 생장점 제거가 아티초크(Cynara scolymus L.)의 생육 및 수량에 미치는 영향)

  • Seong, Ki-Cheol;Kim, Chun Hwan;Son, Danial;Lim, Chan Gyu;Cheon, Sung Jong
    • Journal of Bio-Environment Control
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    • v.23 no.1
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    • pp.56-59
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    • 2014
  • Apex removal is a common practice in artichoke cultivation to harvest heads of lateral shoots. This experiment was carried out to investigate the effect of apex removal by different planting times on the growth and yield of Artichoke (Cynara scolymus L.) in open field. Two treatments (apex removal and no apex removal) at three different planting times ($1^{st}$: Sep. 27, 2011, $2^{nd}$: March 29, 2012, and $3^{rd}$: Sep. 21, 2012) were tested using 'Green Globe' variety. There was no difference in the head characteristics and the number of harvested head between the treatments. The head weight was heavier in no apex removal of 242.7 g than the apex removal of 170.8 g. The yield also increased in no apex removal by 25% (1,249 kg/10a) compared to the apex removal of 997 kg/10a at the first planting time. At the second planting time, there was no difference in the head weights between the treatments. But the number of the harvested head was higher in no apex removal with 10.8 than 8.2 of the apex removal. The yield of no apex removal was 2,660 kg/10a, which was higher than 1,848 kg/10a of apex removal. At the third planting time, the head weight increased in no apex removal with 253.5 g compared to the apex removal with 218.7 g. The yield of no apex removal was 1,405 kg/10a, which was higher than 1,148 kg/10a of the apex removal. All the tests at 3 different planting times showed that the artichoke yields were higher in no apex removal than in apex removal. Therefore, it is desirable to cultivate artichoke without removing the apex for the higher yield and labor saving in open-field cultivation in Jeju island.

Studies on the Eradication of Pedicularis spp., Alternate Host of White Pine Blister Rust with Several Herbicides (제초제처리(除草劑処理)에 의(依)한 잣나무털녹병(病) 중간기주(中間奇主)(송이풀류(類)) 제거시험(除去試験))

  • Yi, Chang Keun;Kim, Hyun Joong;Yeo, Woon Hong
    • Journal of Korean Society of Forest Science
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    • v.51 no.1
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    • pp.51-55
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    • 1981
  • The study was conducted during 1975-1980 to evaluate the performance of several herbicides in controling alternate host, Pedicularis spp., of white pine blister rust disease in forest stands. The results obtained were summarized as follows. 1. It was found that Roundup was effective and most suitable herbicide in the fosest stands except that it accompanied some chemical injuries to neighboring plants. 2. As for the reduction density on the plot treated with Roundup was 100% and 65-91% respectively when investigated one and two years after its treatment on Pedicularis spp. 3. Another four chemicals, 2.4-D, Gramoxone, 2.4. 5-T and Para-col killed stems and leaves of Pedicularis spp. at the year treated, but appeared again next spring. 4. Eradication effectiveness in terms of spraying season was proved to be that late August was better than late May or June.

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The Effect of Early Removal of Mesiodens for the Correction of Central Incisor Rotation (정중과잉치 조기 발거에 따른 상악 영구중절치 회전의 개선)

  • Lee, Jueun;Kim, Youngjin;Kim, Hyunjung;Nam, Soonhyeun
    • Journal of the korean academy of Pediatric Dentistry
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    • v.41 no.1
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    • pp.64-71
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    • 2014
  • Mesiodens is defined as a supernumerary tooth in the premaxillary region. It can cause several clinical manifestations in normal eruption and position of adjacent teeth, such as displacement and impaction. Although the mesiodens accompanied by a clinical complication is indicated for removal, the optimal time of mesiodens removal is still controversial. Some authors suggest immediate intervention defining the removal of mesiodens as soon as possible after the first detection. On the other hand, others recommend delayed intervention which denotes the removal of mesiodens after complete root development of adjacent teeth. This case report is presented with three cases of spontaneous correction and proper alignment of rotated maxillary central incisors by extraction of mesiodens when the crowns of rotated incisors were completely formed while the roots of them were at an early developmental stage.

Effects of Source-Sink Alteration on Dry Matter Accumulation and Protein Content in Soybean (콩에서 Source-Sink변경이 건물축적과 단백질함량에 미치는 영향)

  • Rac Chun, Seong;Ji Hee, park;Sei Joon, Park;Jae Young, Cho
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.40 no.6
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    • pp.723-730
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    • 1995
  • Effects of leaf and pod removal on changes in dry weight and protein content of soybean [Glycine max (L.) Merr.] cultivar 'Hwangkeumkong' were measured at the research farm of Korea University in 1992. The upper 40% and lower 60% of leaves and pods were subjected to treatments at the growth stage of beginning pod(R3). Leaf dry weight of lower part was increased by upper leaf-lower pod removal, but seed dry weight of upper part was decreased. The upper leaf-lower pod removal also increased stem dry weight and decreased upper pod dry weight. Protein content was higher in lower leaves than upper leaves. Seed protein contents were decreased both upper leaf-lower pod removal and lower leaf-upper pod removal treatments. The upper leaf-lower pod removal showed the highest leaf and the lowest seed protein quantities among the five treatments. These results indicated that protein sources were moved from lower to upper parts but weak in remobilization from leaves for the long distance translocation during the reproductive growth period of soybean plants.an plants.

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The Comparative Study of Software Optimal Release Time Based on Intensity Function property (강도함수 특성에 근거한 소프트웨어 최적 방출시기에 관한 비교 연구)

  • Kim, Hee-Cheul;Park, Hyoung-Keun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.11 no.4
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    • pp.1239-1247
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    • 2010
  • In this paper, we were researched decision problem called an optimal release policies after testing a software system in development phase and transferring it to the user. The applied model of release time exploited infinite failure non-homogeneous Poisson process This infinite failure non-homogeneous Poisson process is a model which reflects the possibility of introducing new faults when correcting or modifying the software. The intensity function used Gompertz, Preto and Log-logstic pattern which has the efficient various property. Thus, optimal software release policies which minimize a total average software cost of development and maintenance under the constraint of satisfying a software reliability requirement becomes an optimal release policies. In a numerical example, after trend test applied and estimated the parameters using maximum likelihood estimation of inter-failure time data, estimated software optimal release time.