• Journal of Life Science
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• v.27 no.5
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• pp.530-535
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• 2017

Mitochondria were isolated from bird and mammals. The activity of monoamine oxidase (EC 1.4.3.4) was then measured to identify mitochondrial isolation. Lactate dehydrogenase (EC 1.1.1.27, lactate dehydrogenase, LDH) isozymes in mitochondrial fractions were analyzed by biochemical and immunochemical methods. The activity of mitochondrial LDH was lower in mammals than in bird. Therefore, the role of mitochondrial LDH seems to be more important in bird than in mammals. The concentration of protein in all tissues of bird and mammals was less in the mitochondria than in the cytosol. In the cytosol of mice and golden hamsters, testis-specific LDH $C_4$ isozyme was expressed in testis in addition to the LDH $A_4$, $A_3B$, $A_2B_2$, $AB_3$, and $B_4$ isozymes. A single LDH AB hybrid isozyme was expressed in the chicken mitochondria. In mammals, mitochondrial LDH isozymes were differed according to tissues. LDH $A_4$ and testis-specific LDH $C_4$ isozymes were expressed in the mitochondria of mice. The mitochondrial testis-specific LDH $C_4$ isozyme was expressed only in the mice. In the golden hamster mitochondria, the LDH $B_4$ isozyme functioned as a lactate oxidase. As our results show, the mitochondrial LDH seemed to be playing the different role in the bird and mammals in relation with their metabolic conditions and habitats.

• Proceedings of the KGS Conference
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• 2002.05a
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• pp.120-123
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• 2002

본 연구는 농천어메니티의 개념을 토대로 농촌주민과 도시민의 가치라고 할 수 있는 정주기능으로서의 생활자원화와 방문가치로서의 관광자원화로 구분하고, 이를 토대로 도시민과 농촌주민, 전문가들을 대상으로 농촌자원의 중요도와 순위적 관계를 분석하였다. 구체적인 연구결과를 살펴보면 다음과 같다. 첫째, 농촌자원을 자연적 자원과 문화적 자원, 사회적 자원으로 구분하여 37개 자원을 설정하였다. 이러한 농촌자원은 농촌어메니티를 형성하거나 형성에 영향을 미치는 대상이라고 볼 수 있다. 둘째, 이러한 농촌자원은 농촌주민과 도시민이 지닌 가치에 기초하여 생활자원적 가치와 관광가치적 가치를 동시에 혹은 차등적으로 지닐 수 있다. 이러한 관점은 농촌자원에 대한 중요도 평균에 대한 차이검증(t-test)을 수행한 결과 전체 자원의 56.8%에 해당하는 21개가 유의미한 차이를 보인 결과에 의해 지지를 받는다. 셋째, 농촌자원에 대한 순위적 상관관계분석에서도 전문가와 전체집단에서 생활자원과 관광자원에 대한 순위상관관계가 의미가 없는 것으로 나타나 위의 두번째 결과를 지지하고 있다. 이러한 결과는 농촌자원의 개발 방향과 가치부여가 자원의 속성에 따라 차등적으로 진행되어져야 함을 보여준다. 다섯째, 농촌자원의 유형을 생활자원화와 관광자원화에 대한 중요도의 표준화 점수에 기초하여 4개 유형(유형 I~IV)으로 구분하였다. 이러한 유형별 자원중요도를 고려하여 농촌마을의 개발목적에 비추어 생활자원형이나 관광자원형과의 연계를 통해 마을의 정체성과 특성화에 기여할 수 있도록 해야 할 것이다.(요약 및 결론에서 발췌)를 고지도와 지리지.읍지를 통하여 살펴봄으로써 변한 것과 변하지 않는 것을 분석하여 시대상의 복원과 지역사회의 정체성 확립에 일조하고자 한다.곡물의 여러 줄기가 합수하고 물이 역류하지 않으며 잘 감아도는 곳으로 표현할 수 있다 이러한 명당의 기본적인 원리는 장풍(藏風)과 득수득파(得水得破)이다. 장풍과 득수란 '국'(局), 즉 산줄기와 수계로 이루어진 일정한 범위 속에 만물이 생태계 속에서 살아가는 모습인 '체형(體形)'의 의미가 나타나야 한다는 것이다. 그러나 이러한 의미는 시대에 따라 달라질 수 있는 것이다.면적의 52%를 점유하여 감자 재배지의 핵심지를 이룬다. 4. 한국 감자 재배지역 중심지의 지리적 특징은, 높은 산지지역의 산록완사면에 밭작물로 재배된다는 점과 교통이 불편한 지역으로서 도시화와 산업화 지역의 그늘 지역이 대부분이다. 강원도의 감자 재배지는 감자재배에 적합한 자연환경과 화전농업의 전통, 감자 재배기술의 전파, 중앙정부와 지방정부가 지원하는 각종 연구소 분포와 영농지도, 씨감자 생산과 협동조합의 판로 개척, 도로 개설과 포장 등의 인문지리적 요인이 영농조건을 개선하고 감자 판매를 위한 시장접근을 용이하게 하여, 남한 최대의 감자 재배지역을 형성하였다. 제주도는 산지지형과 따뜻한 기온으로 2기작이 가능하고, 감자가공 공장설립과 교통발달에 따른 육지 시장과의 접근이 용이해졌기 때문에 남한에서 2번째로 큰 감자재배지역이 되었다.(요약 및 결론에서 발췌)그람양성균에서 효과적이었으며, 농도별 항균력시험 결과 농도가 증가할수록 비례하여 저해율도 증가함을 알 수 있었다. 첨가농도를 달

• Tuberculosis and Respiratory Diseases
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• v.46 no.1
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• pp.96-102
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• 1999

A 40-year-old female presented with fever and cough which had lasted for 3 months. Three years previously systemic lupus erythematosus had been diagnosed, and at that time 10mg of prednisone per day was prescribed. Crackles were heard at the base of the lung and her chest roentgenogram revealed diffuse infitrates in both lower lung fields. Despite of empirical antibiotics and antituberculous medication consisting of isoniczid, rifampicin and ethambutol, the patients condition deteriorated. We performed open lung biosy and numerous nodules with necrosis as well as acid fast bacilli were observed. After biochemical tests and sequencing, the organism was identified as Mycobacterium abscessus. The patient was treated with imipenem and amikacin ; patient's condition, as indicated by both symptoms and roentogenogram, subsequently improved.

• Journal of The Korean Dental Society of Anesthesiology
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• v.10 no.2
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• pp.159-165
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• 2010

배경: 매복지치의 수술적 발치 시 propofol과 remifentanil을 이용한 목표조절농도주입(Target controlled infusion) 의식하 정주진정법의 적절한 주입농도를 제시하고 그 안전성을 평가하고자 하였다. 방법: 매복지치의 수술적 발치가 예정된 미국마취학회 신체 등급 분류 1, 2에 속하는 15-65세, 142명(여 83명, 남 59명)의 환자를 대상으로 소급 연구하였다. 환자는 수술 전 목표조절농도주입법을 이용한 의식하 진정법 사용에 관한 동의서를 작성하였다. 정맥내 삽관을 시행하고 수액을 공급을 시작하고, 4-5 L/min의 산소를 비관을 통해서 공급하였다. Propofol과 remifentanil의 초기 목표 혈중농도는 각각 $0.5\;{\mu}g/ml$와 1.0 ng/ml로 정하였다. 수술 중, 환자의 불안 통증 정도에 따라 목표농도를 조절하였으며 최저 농도와 최대 농도, 평균 농도, 주입된 총 용량을 기록하였다. 또한 수축기혈압과 맥박 수, 산소포화도, 호기 말 이산화탄소량을 수술 시작 전, 수술 중 5분 간격으로 확인하고 기록하였다. 모든 측정치는 평균 $\pm$ 표준편차나 환자의 수, 초기 측정치에서의 백분율 편차로 표시하였다. 결과: 수술 동안의 목표 혈중농도의 평균은 propofol은 $0.54{\pm}0.11\;{\mu}g/ml$이고, remifentanil은 $1.11{\pm}0.30\;ng/ml$였다. 수술 중 조절된 최대농도는 propofol은 $0.6{\pm}0.23\;{\mu}g/ml$이고, remifentanil은 $1.3{\pm}0.63\;ng/ml$였다. 이는 의식하 진정에 해당되는 범의의 농도라고 할 수 있겠다. 진정동안 환자의 언어적 의사소통은 유지 되었으며 산소 포화도는 4-5 L/min 의 산소 보충 하에 98%이상으로 유지되었다. 수축기 혈압과 맥박은 대부분의 환자에서 정상변위범위(${\pm}20%$)내에서 유지되었다. 결론: 본 연구는 목표조절 농도주입 의식하 진정에서 사용된 농도(propofol $0.5\;{\mu}g/ml$, remifentanil 1.0 ng/ml)는 안전하게 의식하 진정을 가능한 것을 보여준다. 이는 치과 치료 시 목표조절농도 주입의식하 진정법에서 적절한 목표농도를 제시한다.

• The Korea Journal of Herbology
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• v.31 no.5
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• pp.71-78
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• 2016

Objectives : Diabetic nephropathy is one of the most common chronic complications of diabetes and a leading cause of end-stage renal failure in the world. Mesangial cell proliferation is known as the major pathologic features such as glomerulosclerosis and renal fibrosis. Wiryeongtang (WRT) is a well-known traditional herbal formula as therapeutic agents for chronic edema and dysuresia of renal homeostasis. In the present study, we investigated whether WRT inhibits high glucose (HG)-induced renal dysfunction by TGF-β/Smads signal regulation in cultured mesangial cells.Methods : Inhibitory effect of WRT (10-50 ㎍/ml) on HG-stimulated mesangial cells proliferation and dysfunction were evaluated by [³H]-thymidine incorporation, Western blot, and RT-qPCR.Results : WRT significantly decreased HG-accelerated thymidine incorporation in human renal mesangial cell in a dose-dependent levels. WRT induced down-regulation of cyclins/CDKs and up-regulation of CDK inhibitor, p21waf1/cip1 and p27kip1 expression. In addition, HG enhanced expression of dysfunction biomarker such as collagen IV and CTGF, which was markedly attenuated by WRT. WRT decreased TGF-β1 and Smad-2/Smad-4 expression, whereas increased Smad-7 expression under HG. Furthermore, WRT inhibited HG-induced inflammatory factors level such as ICAM-1 and MCP-1 as well as NF-κB p65 nuclear translocation and intracellular ROS production.Conclusions : These results suggested that WRT may alleviate mesangial proliferation and inflammation possibly involved in renal fibrotic process, further diabetic nephropathy through disturbing TGF-β1/Smad signaling and NF-κB/ROS pathway. Thus, WRT might prove to be effective in the treatment of renal dysfunction leading to diabetic nephropathy.

• Applied Microscopy
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• v.37 no.3
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• pp.167-174
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• 2007

Heat shock protein (HSP) 70 functions as a molecular chaperon and reduces stress-induced denaturation and aggregation of intracellular proteins. Erythropoietin (EPO) plays an important role during acute renal failure repair process by rapidly correcting anemia and enhancing renal tubular regeneration. The purpose of this study was to examine the effect of EPO treatment on renal HSP70 expression. Male Sprague-Dawley rats were injected rHUEPO. Kidney were preserved by in vivo perfusion with paraformaldehyde-lysine-periodate (PLP) and processed for immunohistochemistry and electron microscopy. In control kidney, HSP70 was expressed in the cortex, outer medulla and inner medulla. Especially, HSP immunoreactiviy was mainly founded in descending thin limb of outer medulla and inner medullary collecting duct. In EPO treated kidney, HSP70 expression markedly increased in the descending thin limb of outer medulla and newly detected in cortical collecting duct. Electron microscopy showed the presence of HSP immunoreactivity on the intracelluar vesicles and Golgi complex of descending thin limb and cortical collecting duct. These findings suggest that EPO treatment leads to new production of HSP70 in renal tubular cells, and induction of HSP70 by rHuEPO is causally related to protective function.

• Journal of the Korean Society of Marine Environment & Safety
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• v.21 no.6
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• pp.637-644
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• 2015

In order to estimate harbor tranquility, it is needed to simulate wave propagation in a harbor by using both methods under abnormal wave condition and normal wave condition. The problem is the latter case was not simulated in the statement for the Sea Area Utilization Conference. As harbor calmness about normal wave condition has the same meaning as harbor serviceability, in order to assess harbor tranquility, it is needed to survey wave data for long periods but the survey was not done by reason of a lack of budget and shortage of time for plan. It is more important to make a plan for minimizing environmental impact and to assess an improvement of fisherman's living environmental as the assessment of the harbor serviceability is related with the propriety of the plan. In order to assess it, it is needed to understand it clearly, survey for long period of wave data, and clarify the procedure for computation of it. And also providing wave data like tide and tidal current data from KHOA (Korea Hydro graphic and Oceanographic Agency) and making a guideline for assessing it are needed.

• Journal of Service Research and Studies
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• v.7 no.4
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• pp.39-50
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• 2017

As a comparative study on the value of design concept between service provider (Developer) and service receiver (Customer) group, the aims of this study was to present a service model focused on Continuous positive airway pressure (CPAP) based on U-health care and user environment. Double Diamond-based methods led to a case study that conducted jointly by the department of otolaryngology in U hospital, Ulsan. By targeted on Sleep Apnea patients from the stage of diagnosis to CPAP treatment, variety of meaningful experiences have been extracted and classified into several types of customers. In the final analysis of the 13 service scenario models, Kano Satisfaction and Potential Customer Satisfaction Improvement Index (PCSI) were conducted with the customers, yet Score Model and Concept Position were evaluated by the service provider groups who engaged in development services. The results of this study showed that 7 items in the total of 12 items reached a consensus of viewpoint on value between service receiver and service provider, which the attributes of the service model are based on user environment. Whereas the other 5 items showed the divergent viewpoint on value which included the attributes of U-healthcare service model.

• Journal of Veterinary Clinics
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• v.26 no.6
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• pp.574-577
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• 2009

The purpose of this study is to evaluate gastric motility of conscious minipigs with ultrasonography from contraction number of the pyloric antrum and gastric emptying time (GET). Five-month-old, clinically healthy minipigs weighing 13.5-15 kg were used for this experiment. Assessment of gastric motility was performed using saline (10 ml/kg) in all five minipigs. Contraction number of the pyloric antrum for 3 minutes and gastric emptying time were calculated. Gastric emptying time was considered to be the time at which the antral area returned to the basal value. The mean value of contraction number of the pyloric antrum before feeding was 1.60${\pm}$2.30 (mean${\pm}$SD) for 3 min and the overall mean value after saline administration was 5.40${\pm}$3.62 for 3 min. The mean value of GET by area was 58.06${\pm}$5.23 min. This data can be used as basic database for evaluation of gastric motility in minipigs as normal and various experimental models.

• Journal of Veterinary Clinics
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• v.31 no.5
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• pp.449-453
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• 2014

A 2-year-old, intact male, Persian exotic cat, weighing 2 kg with a history of a palpated abdominal mass was admitted to Veterinary Medical Teaching Hospital, Seoul National University. On routine complete blood count (CBC) and serum biochemical analyses, there were anemia and 0.45 of albumin to globulin ratio. A feline infectious peritonitis (FIP) virus antibody test kit was negative. Radiography, ultrasonography and computed tomography (CT) were performed. A markedly enlarged abdominal mass was found. On cytologic examination of this mass, it was diagnosed as alimentary lymphoma or pyogranulomatous inflammation. Chemotherapeutic treatment for intestinal lymphoma was provided for several weeks, but the mass size was increased and clinical signs were not improved. The cat died six days after discontinuing chemotherapy. On postmortem examination, the definitive diagnosis was FIP. This case describes a cat with FIP in which an abdominal tumor had been suspected clinically.