• Journal of Life Science
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• v.14 no.4
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• pp.702-707
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• 2004

In native-polyacrylamide gel electrophoresis of Pangasius polyuranodon, the lactate dehydrogenase (EC 1.1.1.27, LDH) $A_4$, $A_3$B, $A_2$$B_2$,$AB_3$ and $B_4$ isozymes were expressed in various tissues. The LDH $A_4$ and liver-specific $C_4$ isozymes were expressed in the tissues of Hypostomus Plecostomus. The bands of LDH in skeletal muscle, heart and eye tissues were not detected while one band was detected in kidney and liver, and four bands were detected in brain. The detected one band in liver was identified as alcohol dehydrogenase and an anodal band of skeletal muscle was identified as nothing dehydrogenase. The LDH in skeletal muscle, heart and eye might function as pyruvate reductase. The degree of inhibitions of LDH in skeletal muscle and heart of P. polyuranodon by 10 mM pyruvate were measured 57.6% and 73.8%, respectively. However, those of LDH in tissues of H. plecostomus were measured 52.7-61.8% so tissue specificity did not appear. Therefore, H. ple-costomus might be more acclimated to hypoxic environment by anaerobic metabolism of LDH iso-zymes than P. polyuranodon.

• Journal of Life Science
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• v.14 no.4
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• pp.708-715
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• 2004

The lactate dehydrogenase (EC 1.1.1.27, LDH) in liver of Lempetra japonica was purified in buffer of affinity chromatography. The liver-specific $C_4$ isozyme of Gadus macrocephalus was purified by heat treatment, affinity chromatography, and DEAE-Sephacel chromatography. The liver-specific $C_4$ isozyme was eluted in a buffer containing NAD+ and was coeluted with $B_4$isozyme in plain buffer of affinity chromagraphy. Liver-specific $C_4$ isozyme in G. macrocephalus was the most thermostable, and$B_4$isozyme was more stable than $A_4$. The LDH in the fraction of pH 7.45 purified from the liver of L. iaponica by chromatofocusing was more inhibited by pyruvate than purified LDH. The optimum pH of the LDH isozyme in the liver of L. japonica was 7.5 and that of liver-specific$C_4$ isozyme was 8.5. The LDH in liver of L. japonica made complexes more with antibody against Coreoperca herzi$A_4$ and liver-specific $C_4$ than with that against eye-specific $C_4$. Therefore, the structure of the LDH in liver of L. japonica might be similarly evolved to that of subunit A and liver-specific $C_4$ isozyme in liver tissue of G. macrocephalus. The evolution rate of subunit C is faster than that of subunit A. LDH in liver of L. japonica has not one isozyme but isozymes and it was also found out to have not only subunit A and B but also subunit C.

• Journal of the Korea institute for structural maintenance and inspection
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• v.22 no.1
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• pp.137-146
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• 2018

On September 12, 2016, the Gyeongju District was strongly shaken with M=5.8, which was the largest one since measured by the actual seismometer in Korea, and some buildings were damaged. The field survey of reinforced concrete school buildings in the affected area was carried out, and their residual seismic capacities(R) were estimated based on the Japanese Standard for post-earthquake damage evaluation. In this study, the M school, which was greatly damaged by the 2016 Gyeongju Earthquake, was selected, and its damage level was evaluated on the basis of the Japanese Standard. The seismic capacity of the M school was also evaluated using the nonlinear dynamic analysis, and relationships between its damage level and seismic capacity was also conducted to investigate causes of earthquake damage. The damage level of M school was classified into light with R=88.2%. The result of the dynamic analysis agreed reasonably well with the damage of M school sustained by the 2016 Gyeongju earthquake. This will provide fundamental data for earthquake preparedness measures, such as the seismic rehabilitation of low-rise reinforced concrete buildings in Korea.

• Journal of the Korea institute for structural maintenance and inspection
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• v.22 no.1
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• pp.99-106
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• 2018

This study presents experimental investigation on the residual capacity of fire-damaged voided slabs according to loading conditions. In this study, two voided slab specimens were fabricated, and heated by ISO standard fire during 120 minutes with different loading conditions of presence of loading. These specimens were cooled down to room temperature, and the residual capacity of fire-damaged voided slabs was investigated. Based on test results, thermal distribution of voided slab through the depth of concrete sections is different by the loading conditions. The temperature of loaded specimen is rapidly elevated through the whole depth of concrete sections compared to the unloaded specimen. The residual strength of fire-damaged voided slab specimens are 60% and 66% of that of voided slab specimen without fire damage, and the residual stiffness of fire-damaged voided slab specimens decreases by 15%~23% of that of voided slab specimen without fire damage. In case of voided slab specimens subjected ISO standard fire, the loaded specimen shows the decrease of 10% in the residual strength and the decrease of 15% in the residual stiffness compared to the unloaded specimen. It seems to result from higher temperature of bottom reinforcements in the loaded specimen due to the cracks, and more extensive damage on concrete cover of reinforcements by spalling process according to load level.

• Korean Journal of Environmental Biology
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• v.17 no.3
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• pp.263-270
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• 1999

In order to identify the morphological changes of tissues in mouse after irradiation. We have observed the redistribution of LDH isozymes and the morphological changes of skeletal muscle, heart, kidney, liver and testis in mouse according to variation amount with the time after the 1 Gray and 3 Gray irradiation each. As a result of H-E (hematoxylin-eosin) stain, the apoptotic bodies were more easily observed in the liver than the other tissues and the quantity of the apoptotic bodies was proportionated to radiation amount. The number of apoptotic bodies was shown the highest at 1 day in most tissues and at 7 day in testis after irradiation. TUNEL (terminal deoxyribonucleodtidyl transferase-mediated dUTP-digoxigenin nick end labeling) staining was shown the same results as H-E staining. After the irradiation, the protein content was reduced in tissues except kidney. And protein content was reduced in all tissues at the initial period of 2 hours after 3 Gy irradiation. But it increased at 7 days after irradiation. LDH (EC 1.1.1.27, lactate dehydrogenase) activity was increased mostly in tissues at the early stage after 1 Gy irradiation. The maximum activity was detected earlier stage after 1 Gy irradiation than 3 Gy irradiation. The activity of LDH $A_4$ isozyme was decreased in the skeletal muscle, heart, kidney, and testis. The activity of $B_4$ and $A_2$$B_2$ sozyme was increased in the skeletal muscle and heart, and the activity of heterotetramer isozyme was increased in kidney The activity of $A_4$ isozyme in liver was detected high level and the activity of isozyme including subunit C elevated in testis. Therefore, LDH isozyme seems to play a role of lactate oxidase in most tissues except liver after irradiation. These data support that LDH isozyme is predomintly involved in the aerobic metabolism.

• Journal of Science Education
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• v.34 no.1
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• pp.93-104
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• 2010

The purpose of this study was to investigate the high school students' interests and and perceptions about the soil concepts. The data were obtained from 72 boys and 82 girls at a high school in Daegu area. The questionnaires were developed 8 questions for students' interest and 10 questions for students' understanding. The results of this study were as follows. In the examination of students' interest on the soil concepts, boys showed 3.2point(64.9%) out of 5point and girls showed 3.2point(64.6%) out of 5 point. Furthermore, a percentage of correct answers for understanding on the soil concepts was 47.4%. In detail, Boys had 48.2% and girls had 46.6%, boys showed higher understanding about soil concepts than girls. And students understood soil concepts by a visible side, to approach the soil concepts from a biological point of view, rather than a geologic one. Also high school students didn't think soil concepts to consist of minerals and rocks from a geologic point of view. Correlation between interest and understanding on the soil concepts was very weak by showing r=.170(p<.05).

• Journal of Science Education
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• v.34 no.1
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• pp.12-22
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• 2010

The purpose of this study was to investigate the effect of concept sketches on the understanding and scientific attitude in high school student's learning of weather change. Among the various fields of meteorology, especially in weather change, we often deal with the change of the spatiotemporal change in an abstract way. So making use of 'Concept Sketches'- simplified sketches which represent the main features, principles, processes and interrelationships of the learning contents using some concise explanations, signs and terms - could help the students learn the phenomena of weather change efficiently. This study's aim was to check up the effect and analyze the results of the lesson including the concept sketches. As a result of this study, concept sketches group showed significant improvement compared to the other groups in understanding of weather change and in scientific attitude, too. In students' recognition research of concept sketches showed that students found the class more interesting with improved concentration and had a chance to review through concept sketching, which is helpful for their learning. Considering the above research results, the study which applies concept sketching required the students to actively process their knowledge, and had a positive effect on the understanding of weather changes. Most of all, drawing the pictures which is a familiar activity helped the students to take part in the class eagerly.

• Journal of Food Hygiene and Safety
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• v.31 no.4
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• pp.294-298
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• 2016

Natural products are attractive as the source of new drug development. Especially, numerous unknown marine bioresources are an object of attention because the ocean occupies three fourth of the earth. Survival of marine bioresources in extreme environment may induce the production of biological active compounds. As previous study, we examined over 40 specimens of marine sponges collected from Micronesia and screened their anti-proliferative activities in various cancer cell lines. Among them, we investigated Coscinoderma sp.'s activity and mechanism in human colon carcinoma HCT116 and RKO cells. Furthermore, we also used the p53-knockout of HCT116 cells and the p53 loss of RKO cells for elucidating the role of p53. Coscinoderma sp. inhibited cellular viability independently of the p53 status. Therefore, we compared the expression level of cell death-related proteins by Coscinoderma sp. in HCT16 and in HCT116 p53KO cells. Coscinoderma sp. increased p53 level and NOXA levels and induced apoptosis under the condition of p53 existence. On the other hand, Coscinoderma sp. increased p21 and mTOR levels in HCT116 p53KO cells. These results suggest that Coscinoderma sp. induced anti-proliferation effect through different pathway depending on p53 status.

• Korean Journal of Ecology and Environment
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• v.39 no.2 s.116
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• pp.178-186
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• 2006

Community structures of fish in six small eutrophied lakes, located in Gyeonggi province, Korea were investigated from September 2003 to August 2004. Total number of fish species was 33 species of 13 families in which five Korean endemic species such as Rhodeus uyekii, Squalidus gracilis majimae, Abbottina springeri, Iksookimia koreensis, and Odontobutis interrupta were found. Dominant species in the lakes was Hemiculter leucisculus. Both Zacco platypus and Pseudorasbora parva were numberous as subdominant species. In particlilar, Micropercops swinhonis known as partly distributed in the Jeonlabukdo was first recorded in Lake Wangsong and Heungbu which are located in the Gyeonggi province. In lake Myukwoo, Wangsong, and Heungbu, the proportion of Pelagic and omnivorous fish were high, reflecting that fish habitat is poor. A CPUE based fish production was the lowest in Lake Geumgwang of which lake is relatively favorable, whereas it was higher in eutriphied lakes suchs as Dukwoo, Myukwoo and Heungbu lakes.

• Journal of Life Science
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• v.21 no.11
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• pp.1565-1572
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• 2011

Eye-specific lactate dehydrogenase (EC 1.1.1.27, LDH) $C_4$ isozyme in the eyes of greenlings (Hexagrammos otakii) was successfully purified by affinity chromatography and continuous-elution electrophoresis. The molecular weight of the purified eye-specific LDH $C_4$ isozyme was 154.8 kDa, as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Optimal pH for enzymatic reaction of the eye-specific LDH $C_4$ isozyme was pH 8.5. $K^{PYR}_m$ value of the purified eye-specific LDH $C_4$ isozyme was $1.88{\times}10^{-5}$ M using pyruvate as a substrate. These results indicate that we must consider pH when measuring eye-specific LDH $C_4$ isozyme activity. The eye-specific LDH $C_4$ isozyme had a higher binding affinity for the substrate as a pyruvate than LDH A4 isozyme. Antibodies produced against the purified eye-specific LDH $C_4$ isozyme may be used in the diagnosis of several human diseases and in comparative physiological studies of fishes.