• Journal of Veterinary Clinics
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• v.34 no.2
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• pp.156-160
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• 2017

We investigated the effect of ethylene glycol and antioxidants such as taurine, hypotaurine and trehalose with extenders during cryopreservation of Korean Jeju Black Bull spermatozoa. The cryopreservation of freshly collected spermatozoa was conducted with four different conditions. As a control, spermatozoa were cryopreserved with Tris egg-yolk extenders added 5% ethylene glycol (EG). Taurine (20 mM), hypotaurine (20 mM) and trehalose (20 mM) were individually added into tris egg-yolk extenders with 5% EG. After thawing of frozen spermatozoa with four different conditions, sperm viability, motility, acrosomal integrity, and membrane integrity were investigated. The significant (p < 0.05) improvement of sperm viability showed in all antioxidant treated thawed spermatozoa (taurine; $68.1%{\pm}4.4$, hypotaurine; $69.2%{\pm}6.7$ and trehalose; $68.0%{\pm}4.4$) when compared to control ($63.4%{\pm}5.6$). Neither positive nor detrimental effects of three antioxidants were shown sperm motility after thawing. The results of hypo-osmotic swelling test showed that the membrane integrity of taurine, hypotaurine or trehalose treated thawed spermatozoa ($64.1%{\pm}5.4$, $61.5%{\pm}3.7$ and $59.0%{\pm}4.0$, respectively) had significantly (p < 0.05) higher rate of the swollen sperm compared to control ($53.7%{\pm}9.7$). Hypotaurine treated frozen-thawed spermatozoa had siginificantly higher (p < 0.05) F pattern ratio than taurine, trehalose and control treated frozen-thawed spermatozoa. Trehalose added frozen-thawed spermatozoa had significantly higher (p < 0.05) acrosome reaction pattern ratio than taurine and hypotaurine added frozen-thawd spermatozoa. In this study, we found that antioxidants such taurine, hypotaurine and trehalose treatments during cryopreservation process could reduce damage of spermatozoa of Korean Jeju Black Bull and improved sperm capability of fertilization.

• Clinical and Experimental Reproductive Medicine
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• v.32 no.2
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• pp.149-154
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• 2005

연구목적: 정자의 첨체상태는 수정능과 상관관계가 있다. 본 연구는 사람 정자의 동결보존 시 Fertilization promoting peptide (FPP) 처리가 첨체 유지에 효과가 있는지를 알아보고자 실시하였다. 연구재료 및 방법: 사람 정자는 정액검사를 의뢰한 시료를 사용하였으며, 적정농도를 조사하기 위하여 25, 50, 100 nM FPP를 신선정자에 처리한 뒤 시간별로 첨체의 변화를 조사하였다. 또한 적정화된 50 nM FPP를 정자의 동결-융해 시에 처리한 뒤 첨체 변화를 조사하였다. 첨체 변화는 FITC - pisum sativum lectin (PSA) 염색방법을 이용하여 조사하였다. 결 과: FPP 농도 변화와 처리시간에 따른 사람 정자의 첨체 변화를 조사하였던 바, 50 nM FPP 처리군에서 대조군보다 높은 온전한 첨체비율을 얻을 수 있었다. 정자의 동결-융해 시, 동결액과 융해액에 50 nM FPP 첨가가 온전한 첨체를 유지하는 비율을 조사하였던 바, 신선 정자의 결과보다는 유의하게 낮지만 무 처리군보다 유의적으로 높은 온전한 첨체를 얻을 수 있는 것을 알 수 있었다. 또한 동결액에만 또는 융해액에만 50 nM FPP 처리를 하더라도 무 처리군보다 유의하게 높은 온전한 첨체 비율을 획득할 수 있음을 알 수 있었다 (p<0.001). 결 론: 사람 정자의 동결보존 시 50 nM FPP 첨가는 자발적으로 발생하는 첨체반응을 억제하고, 온전한 첨체를 유지할 수 있어 수정능 보유에 기여할 수 있을 것으로 사료된다.

• ANNALS OF ANIMAL RESOURCE SCIENCES
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• v.29 no.4
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• pp.166-171
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• 2018

The effect of bisphenol S (BPS) on the viability and production of reactive oxygen species (ROS) was studied in boar sperm and ovarian granulosa cells. Boar semen was incubated in Beltsville thawing solution with either 0 or $5{\mu}M$ BPS for 3 and 6 h. The viability of sperm was analyzed by SYBR14/PI doubling staining, and production of ROS was detected. Ovarian granulosa cells were also treated with BPS for 24, 48, and 72 h. Then, cell viability (0, 5, 10, and $20{\mu}M$) and ROS production (only 0 and $5{\mu}M$ BPS) were assessed. The results showed that, BPS decreased sperm viability at 3 and 6 h, and that BPS increased ROS production (p<0.05). Also, BPS reduced the viability of ovarian granulosa cells (p<0.05), and stimulated ROS production (p<0.05). These results suggest that BPS damages sperm activation and ovarian granulosa cells in the reproductive system.

• Health Policy and Management
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• v.29 no.1
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• pp.40-48
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• 2019

Background: As of July 2015, per diem payment was changed from fee for service Therefore, this study aims to analyse changes in medical charges and medical services before and after enforcement of the palliative care, targeting palliative care wards in a general hospital, and provide basic data needed for development of per diem payment. Methods: The subjects of the study were a total of 610 cases consisting of 351 patients of service fee who left hospital (died) from July 2014 to June 2016 and 259 ones of per diem payment at Chosun University Hospital in Gwangju Metropolitan City. Results: The results are summarized as follows. First, after the palliative care system was applied, benefit medical service charges and insurance increased significantly (p<0.001). As benefit medical service charges increased, benefit private insurance payment increased significantly (p<0.001). Second, after the per diem payment was applied, total private insurance payment to medical institutes decreased significantly (p=0.050) and non-benefit also decreased significantly (p=0.001). Conclusion: It is suggested that additional rewards in the obligatory palliative care items should be continuously remedied and monitored to provide good quality hospice palliative care.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• v.15 no.5
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• pp.387-395
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• 2022

PDRN (Polydeoxyribonucleotide) is a DNA-derived polymer that promotes self-renewal of damaged cells and tissues as a tissue regeneration active material. PDRN is a DNA fragment cut into small sizes by various physical or chemical methods. When administered to the body, PDRN binds and stimulates the adenosine A2A receptor on the surface of tissue cells to promote cell regeneration, accelerate wound healing, and reduce pain. Although PDRN is prepared from testis or semen of fish in most cass, PDRN extraction from various plants species was performed in the present study. Among 7 tested plant species, the highest DNA yield and purity was obtained form mugwort (Chrysanthemum coronarium, C.c), followed by broccoli (Brassica oleracea, B.o). Then, we evaluated the in vitro wound healing capacity of PDRNs prepared from these two selected plants. PDRN from C.c and B.o. significantly stimulated the wound healing process at ㎍/ml range. The present study suggests that PDRN from plant species can be an effective alternative to PDRN from marine organism.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.56 no.4
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• pp.401-410
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• 2023

This study examined the characteristics of eel Anguilla japonica sperm using the CASA (computer-assisted sperm analysis) system and attempted to provide the composition for artificial seminal plasma by regulating of inorganic elements. Sperm samples were first divided into four groups based on motility and progressive motility: (A) 0-10%, (B) 10-40%, (C) 40-70%, and (D) 70-100%. For observing sperm velocity variations, VCL, which is curve motion velocity, showed the highest values in all groups. The directional factor, beat cross frequency, was lower in higher activity groups, showing an opposite correlation with sperm activity. The head sizes of spermatozoa in higher activity groups were significantly larger than those in lower activity groups. The Na⁺ and K⁺ ions were important in the inorganic composition of seminal plasma in this species. Furthermore, regulating the composition in artificial seminal plasma improved the formula compared to the existing element, exhibiting 120 mM Na and 30 mM K when the sperm was conserved for a long time and 120 mM NA and 40 mM K when the sperm was conserved for a short time.

• Analytical Science and Technology
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• v.21 no.1
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• pp.48-52
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• 2008

A new forensic saliva test method using $SALIgAE^{(R)}$ was evaluated in this study. The sensitivity and specificity of $SALIgAE^{(R)}$ were examined and compared to those of other saliva test methods such as agarose gel diffusion method and $Phadebas^{(R)}$ test sheet method. $SALIgAE^{(R)}$ showed high sensitivity and specificity to human saliva in addition to quickness. Moreover modified $SALIgAE^{(R)}$ method was cheap and easy to use in crime scene and DNA laboratory. $SALIgAE^{(R)}$ was very stable at room temperature and had no effect on STR typing.

• Journal of the Korea institute for structural maintenance and inspection
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• v.27 no.5
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• pp.57-66
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• 2023

Asset valuation of social infrastructure is essential for rational decision-making for efficient management of assets. In addition, it can be an indicator for correctly recognizing assets. In general, Korea applies depreciated replacement cost based on the straight-line method to evaluate asset value, yet this is unsuitable for evaluating actual value because it is depreciated at a constant rate over the useful life period. In order to evaluate the asset value considering the performance of the bridge, the performance index of the bridge is estimated using the Weibull distribution. Using the estimated performance indicators and defect index, a new asset value evaluation method is proposed and compared and analyzed with the existing method. The proposed valuation method can take into account the performance of the bridge, so it is judged to be more objective and reasonable than existing method.

• Journal of Embryo Transfer
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• v.18 no.1
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• pp.43-50
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• 2003

The objective of this study was to investigate the effect of cryopreservation by slow and rapid freezing on the sperm motility index, viability and morphology of post-thaw human spermatozoa. After rapid freezing and thawing, sperm motility index was significantly higher (MOT:47.40$\pm$20.06%, VCL : 38.12$\pm$15.58 $\mu$m/s, VSL : 28.19$\pm$14.10 $\mu$m/s, VAP:33.64$\pm$15.15 $\mu$m/s, and HYP 2.77$\pm$2.71%) than slow freezing and thawing(MOT : 43.39$\pm$ 18.79%, VCL .33.91 $\pm$ 13.50 Um/s, VSL . 19.98$\pm$0.88 $\mu$m/s, VAP : 24.60$\pm$11.72 $\mu$m/s, and HYP . 1.33$\pm$1.57% ; P<0.05). But sperm Linearity(LIN) was significantly lower(28.83 $\pm$ 10.35) comparing to the slow freezing method(34.64 $\pm$ 11.36 ; P<0.05). On the other hand, significant difference were not observed MAD, WOB, DNC and DNM by slow and rapid frozen-thawed methods. After rapid freezing and thawing, sperm viability was lower(60 $\pm$ 2.2%) than slow freezing method(62 $\pm$2.1%) and sperm morphology was higher(46$\pm$7.7%) than that(44: 8.3). But there was no significantly These results indicate that rapid freezing method was positive effect of sperm cryopreservation in human.

• Journal of Veterinary Clinics
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• v.24 no.4
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• pp.577-583
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• 2007

It is important to obtain semen with good quality for efficient fertilization and pregnancy. To obtain these semen, various methods have been developed but most of these methods are time consuming and require costly equipment. Therefore, the objective of this research is to investigate the usability of column filtration system as quick and simple method to get sperm with better quality. Ejaculates were obtained from 5 dogs and analyzed with basic quality parameters before each filtration. Sperm concentration was adjusted to $5{\times}10^7/ml$ after dilution. The experimental groups were divided into non-filtered group(control) and filtered groups(glass wool, Sephadex 5% and Sephadex 20%). Ejaculates were filtered through each filter system and assessed by recovery rate of sperm, motility, normal morphology, CFDA/PI stain and plasma membrane integrity(hypo-osmotic swelling test, HOST). The lowest recovery rate of spermatozoa was recorded in glass wool filtration group, followed by 20% Sephadex filtration group(p<0.05). There was no significant difference between control(non-filtered) and 5% Sephadex filtration poop. Also, there was no significant difference of sperm motility assessed under light microscope among experimental groups. Morphological normality of canine spermatozoa was the highest in the glass wool filtration group and the lowest in the 5% Sephadex filtration group with no significant differences versus 20% Sephadex filtration and control group, respectively(p<0.05). Viability of canine sperm assessed by CFCA/PI staining was the highest in the glass wool filtration poop with no significant difference versus the control group, and the lowest in the 20% Sephadex filtration group with no significant difference versus 5% Sephadex filtration group, respectively(p<0.05). HOS values of canine sperm was the highest in the 20% Sephadex filtration group with no significant difference versus 5% Sephadex filtration group, and the lowest in the control poop with no significant difference versus glass wool filtration group, respectively(p<0.05). Therefore, these results indicated that filtration treatment for extended canine sperm would be useful method to get sperm with better quality by trapping the damaged sperm, consequently filter would be physical barrier against injured or immotile sperm.