ANNALS OF ANIMAL RESOURCE SCIENCES (동물자원연구)

INSTITUTE OF ANIMAL RESOURCES (강원대학교 동물자원공동연구소)
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Effects of Bisphenol S on Viability and Reactive Oxygen Species of the Sperm and Ovarian Granulosa Cells in Pigs

Bisphenol S가 돼지정자와 난소내 과립막세포의 생존성과 활성산소에 미치는 영향

  • Lee, Yu-Sub (College of Animal Life Sciences, Kangwon National University) ;
  • Lee, Seunghyung (College of Animal Life Sciences, Kangwon National University) ;
  • Yang, Boo-Keun (College of Animal Life Sciences, Kangwon National University)
  • 이유섭 (강원대학교 동물생명과학대학) ;
  • 이승형 (강원대학교 동물생명과학대학) ;
  • 양부근 (강원대학교 동물생명과학대학)
  • Received : 2018.07.04
  • Accepted : 2018.12.17
  • Published : 2018.12.31
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Abstract

The effect of bisphenol S (BPS) on the viability and production of reactive oxygen species (ROS) was studied in boar sperm and ovarian granulosa cells. Boar semen was incubated in Beltsville thawing solution with either 0 or $5{\mu}M$ BPS for 3 and 6 h. The viability of sperm was analyzed by SYBR14/PI doubling staining, and production of ROS was detected. Ovarian granulosa cells were also treated with BPS for 24, 48, and 72 h. Then, cell viability (0, 5, 10, and $20{\mu}M$) and ROS production (only 0 and $5{\mu}M$ BPS) were assessed. The results showed that, BPS decreased sperm viability at 3 and 6 h, and that BPS increased ROS production (p<0.05). Also, BPS reduced the viability of ovarian granulosa cells (p<0.05), and stimulated ROS production (p<0.05). These results suggest that BPS damages sperm activation and ovarian granulosa cells in the reproductive system.

본 연구는 돼지의 정자와 난소내 과립막세포에서 bisphenol S(BPS)가 생존성과 활성산소 생산에 미치는 영향을 알아보고자 연구하였다. 돼지정액은 0, $5{\mu}M$ BPS를 처리하여 3, 6시간동안 배양하였다. 정자의 생존성은 SYBR14/PI를 이중 염색하여 분석하였으며, 활성산소의 생산을 측정하였다. 또한, BPS(0, 5, 10, $20{\mu}M$)를 과립막세포에 처리하여 24, 48, 72시간동안 처리하였다. 처리 후, 세포의 생존율과 활성산소 생산(단, $5{\mu}M$ BPS)을 측정하였다. 그 결과, 돼지에서 정자의 생존율은 BPS에 의해 감소하였고, 활성산소의 생산은 모든 처리시간에서 증가하였다(p<0.05). 또한 과립막세포의 생존은 BPS에 의해 억제되었고, 활성산소는 유의적으로 증가하였다(p<0.05). 이상의 결과를 토대로, BPS의 노출은 정자의 활성과 번식과 관련된 세포에 나쁜 영향을 미칠 것이다.

Keywords

Acknowledgement

Supported by : 강원대학교

References

  1. Amaral, A., Lourenco, B., Marques, M. and Ramalho-Santos, J. 2013. Mitochondria functionality and sperm quality. Reproduction. 146:163-174. https://doi.org/10.1530/REP-13-0178
  2. Ahsan, N., Ullah, H., Ullah, W. and Jahan, S. Comparative effects of Bisphenol S and Bisphenol A on the development of female reproductive system in rats; a neonatal exposure study. Chemosphere. 197: 336-343.
  3. Crump, D., Chiu, S. and Williams, K. L. 2016. Bisphenol S alters embryonic viability, development, gallbladder size, and messenger RNA expression in chicken embryos exposed via egg injection. Environ. Toxicol. Chem. 35:1541-1549. https://doi.org/10.1002/etc.3313
  4. Gallet, M. and Vanacker, J. M. 2010. ERR receptors as potential targets in osteoporosis. Trends Endocrinol. Metab. 21:637-641. https://doi.org/10.1016/j.tem.2010.06.008
  5. George, V. C. and Rupasinghe, H. P. V. DNA damaging and apoptotic potentials of Bisphenol A and Bisphenol S in human bronchial epithelial cells. Environ. Toxicol. Pharmacol. 60:52-57.
  6. Hashimoto, Y. and Nakamura, M. 2000. Estrogenic activity of dental materials and bisphenol-A related chemicals in vitro. Dent. Mater. J. 19:245-62. https://doi.org/10.4012/dmj.19.245
  7. Ji, K., Hong, S., Kho, Y. and Choi, K. 2013. Effects of bisphenol s exposure on endocrine functions and reproduction of zebrafish. Environ. Sci. Technol. 47:8793-800. https://doi.org/10.1021/es400329t
  8. Kiyama, R. and Wada-Kiyama, Y. 2015. Estrogenic endocrine disruptors: Molecular mechanisms of action. Environ. Int. 83:11-40. https://doi.org/10.1016/j.envint.2015.05.012
  9. Lee, S. H. and Park, C. K. 2015. Effect of magnetized extender on sperm membrane integrity and development of oocytes in vitro fertilized with liquid storage boar semen. Anim. Reprod. Sci. 154:86-94. https://doi.org/10.1016/j.anireprosci.2014.12.015
  10. Liao, C. and Kannan, K. 2013. Concentrations and profiles of Bisphenol A and other Bisphenol Analogues in foodstuffs from the United States and their implications for Human exposure. J. Agric. Food Chem. 61:4655-4662. https://doi.org/10.1021/jf400445n
  11. Liao, C. and Kannan, K. 2014. A survey of bisphenol A and other bisphenol analogues in foodstuffs from nine cities in China. Arch. Environ. Contam. Toxicol. 67:50-59. https://doi.org/10.1007/s00244-014-0016-8
  12. Mahfouz, R. Z., du Plessis, S. S., Aziz, N. Sharma, R. Sabanegh, E. and Agarwal, A. 2010. Sperm viability, apoptosis, and intracellular reactive oxygen species levels in human spermatozoa before and after induction of oxidative stress. Fertil. Steril. 93:814-21. https://doi.org/10.1016/j.fertnstert.2008.10.068
  13. Nourian, A., Soleimanzadeh, A., Shalizar Jalali, A. and Najafi, G. Effects of bisphenol-S low concentrations on oxidative stress status and in vitro fertilization potential in mature female mice. Vet. Res. Forum. 4:341-345.
  14. Pursel, V. G. and Johnson, L. A. 1975. Freezing of boar spermatozoa: fertilizing capacity with concentrated semen and a new thawing procedure. J. Anim. Sci. 40:99-102. https://doi.org/10.2527/jas1975.40199x
  15. Shi, M., Sekulovski, N., MacLean, J. A. and Hayashi, K. 2017. Effects of bisphenol A analogues on reproductive functions in mice. Reprod. Toxicol. 73:280-291. https://doi.org/10.1016/j.reprotox.2017.06.134
  16. Sifakis, S., Androutsopoulos, V. P., Tsatsakis, A. M. and Spandidos, D. A. 2017. Human exposure to endocrine disrupting chemicals: Effects on the male and female reproductive systems. Environ. Toxicol. Pharmacol. 51:56-70. https://doi.org/10.1016/j.etap.2017.02.024
  17. Ullah, H., Jahan, S., Ain, Q. U., Shaheen, G. and Ahsan, N. 2016. Effect of bisphenol S exposure on male reproductive system of rats: A histological and biochemical study. Chemosphere. 52:383-391
  18. Ullah, H., Ambreen, A., Ahsan, N. and Jahan, S. 2017. Bisphenol S induces oxidative stress and DNA damage in rat spermatozoa in vitro and disrupts daily sperm production in vivo. Toxicol. Environ. Chem. 99:953-965. https://doi.org/10.1080/02772248.2016.1269333
  19. Zalmanova, T., Hoskova, K., Nevoral, J., Adamkova, K., Kott, T., Sulc, M., Kotikova, Z., Prokesova, S., Jilek, F., Kralickova, M. and Petr, J. 2017. Bisphenol S negatively affects the meotic maturation of pig oocytes. Sci. Rep. 1:485.