• Title/Summary/Keyword: 정성적 조사

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Acute Toxicity of Ozone on Survival and Physiological Conditions of Olive Flounder, Paralichthys Olivaceus (넙치(Paralichthys olivaceus)의 생존과 생리상태에 미치는 오존의 급성 독성)

  • Kim, Heung-Yun;Oh, Myung-Joo;Jung, Sung-Ju
    • Journal of fish pathology
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    • v.12 no.1
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    • pp.32-41
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    • 1999
  • Ozonization for rearing seawater in land-based culture system has recently been utilized for disinfection of pathogenic microorganisms and improvement of water quality. This study was conducted to examine the effects of total residual oxidants (TRO) in ozone-treated seawater on survival, blood parameters, osmolality and oxygen consumption, and gill tissue of the flounder. Paralichthys olivaceus. Experiments were carried out with the starved flounder of 12~19 cm in total length at $20^{\circ}C$. The 48-hr and 96-hr $LC_{50}$ for the flounder amounts to 26.4 ppb and 22.3 ppb, respectively. With increase of TRO concentration from 24 to 39 ppb, the values of hematocrit, hemoglobin concentration, red blood cell count and osmolality of the flounder with respect to exposure time were significantly elevated, however, the oxygen consumption rates decreased. In the case of the fish exposed to 13 ppb for 96 hrs, blood glucose increased with an elapse of exposure time, while survival rate was 100%. Death apparently resulted by massive destruction of gill lamellar epithelium, severe osmotic imbalance and the lack of oxygen uptake. The results of this experiment indicated that to protect aquaculture organisms, the ozone-treated seawater should not contain any residual oxidants, and that relatively long-term exposure to TRO of low concentration can impact on survival and physiological conditions of the flounder.

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Community Structure of Pinus thunbergii Stand in the Eastern Coast of Gyeongsangbuk-do (경상북도 동해안 곰솔림의 군집구조)

  • Cheon, Kwang-Il;Jung, Sung-Cheol;Youn, Ho-Joong;Byeon, Jun-Gi;Joo, Sung-Hyun
    • Journal of agriculture & life science
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    • v.46 no.4
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    • pp.57-68
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    • 2012
  • This study was conducted to provide basic data for the effective conservation and management in the black pine(Pinus thunbergii) stand which is located in the eastern coast of Gyeongsangbuk-do where the fragmentation of vegetation has been caused by the exploitation and the increase of tourists, and installed the seventy study sites($20{\times}20m$) in the dominant black pine stand. The black pine stand was classified into three groups(P. thunbergii - P. densiflora community, P. thunbergii - Robinia pseudoacacia community, P. thunbergii - P. densiflora-Celtis sinensis community) by a cluster analysis. As a result of Multi-Response Permutation Procedures test, there is significance among the communities. Pinus densiflora, Callicarpa japonica, Juniperus rigida, Rhododendron yedoense for. poukhanense etc. nineteen species were significant by indicator species analysis. The population structure of black pine stand showed that the class of diameter 12 to 26 cm was dominance, which had the inverse hump-shape pattern. Species diversity index(H') of investigated ranged from $1.033{\pm}0.234$ to $1.629{\pm}0.226$ in the woody layer group and from $2.448{\pm}0.457$ to $2.545{\pm}0.318$ $2.174{\pm}0.333$ in the herb layer group.

Laborsaving Effect and Fruit Characteristics of Grape ‘Campbell Early’ According to Pedicel Thinning ('캠벨얼리' 포도의 지경솎기에 따른 작업 절감 효과 및 과실 특성)

  • Kim, Su Jin;Park, Seo Jun;Koh, Sang-Wook;Jung, Sung Min;Hur, Youn Young;Nam, Jong Cheol;Park, Kyo Sun
    • Korean Journal of Plant Resources
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    • v.28 no.2
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    • pp.290-295
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    • 2015
  • The experiment were carried out in 7-year-old ‘Campbell Early’ grape to increase work efficiency by fruit cluster thinning methods (Pedicel and berry thinning). Pedicel thinning, such as labor-saving cluster thinning of grape, was 6.7 fold higher than the berry thinning for work efficiency. The fruit cluster weight and number of berry were lower in the fruit cluster thinning fruits than in the none-fruit cluster thinning, however, the soluble solid content (SSC) was high and titratable acidity (TA) was low in the fruit cluster thinning fruits than in the none-fruit cluster thinning fruits. Therefore, the bruising rate of berries was decreased in the fruit cluster thinning fruits. Quality uniformity by fruit cluster thinning was proper in fruit cluster weight of 350~450 g when SSC and TA in part of lower, middle and upper of cluster was considered.

Analysis of Chemical Constituents of Agastachis Herba and in silico Investigation on Antidiabetic Target Proteins of its Major Compounds (곽향의 성분 분석 및 주요 성분들의 in silico 항당뇨 타겟 단백질 탐색)

  • Choi, Jongkeun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.22 no.4
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    • pp.483-492
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    • 2021
  • Agastachis Herba (AH) to treat anorexia and nausea and its antidiabetic efficacy was recently reported. This study examined the antioxidant activities and chemical constituents of AH and predicted the target proteins of each compound using in silico approaches. The results showed that EC50 values of AH methanol extract for DPPH and ABTS radical scavenging were 78.6 ㎍/mL and 31.0 ㎍/mL, respectively. Compared to the EC50 values of ascorbic acid (9.9 ㎍/mL, 5.2 ㎍/mL), the AH methanol extract possessed excellent antioxidant activities. Rosmarinic acid, tilianin, agastachoside, and acetin were confirmed as the major compounds of extracts by qualitative analysis performed with HPLC-PDA-MS/MS. The antidiabetic target proteins of these compounds were predicted by applying a structural similarity and inverse docking methodology using a DIA-DB server. The resulting target proteins were PPAR-γ, DPP IV, glucokinase, α-glucosidase, SGLT2, aldose reductase, and corticosteroid 11-beta-dehydrogenase, some of which have already been proven experimentally as target proteins. Therefore, the in silico methods can be considered valid. Finally, AH were extracted with various solvents to determine the optimal conditions for the extraction of active components. Methanol among organic solvents and 80% ethanol in ethanol-water mixtures were identified as the most effective solvent for the extraction.

Radioanalytical and Spectroscopic Characterizations of Hydroxo- and Oxalato-Am(III) Complexes (방사분석과 분광학을 이용한 Am(III) 가수분해와 옥살레이트 착물 화학종 연구)

  • Kim, Hee-Kyung;Cho, Hye-Ryun;Jung, Euo Chang;Cha, Wansik
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.16 no.4
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    • pp.397-410
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    • 2018
  • When considering the long-term safety assessment of spent-nuclear fuel management, americium is one of the most radio-toxic actinides. Although spectroscopic methods are widely used for the study of actinide chemistry, application of those methods to americium chemistry has been limited. Herein, we purified $^{241}Am$ to obtain a highly pure stock solution required for spectroscopic studies. Quantitative and qualitative analyses of purified $^{241}Am$ were carried out using liquid scintillation counting, and gamma and alpha radiation spectrometry. Highly sensitive absorption spectrometry coupled with a liquid waveguide capillary cell and time-resolved laser fluorescence spectroscopy were employed for the study of Am(III) hydrolysis and oxalate (Ox) complexation. $Am^{3+}$ ions under acidic conditions exhibit maximum absorbance at 503 nm, with a molar absorption coefficient of $424{\pm}8cm^{-1}{\cdot}M^{-1}$. $Am(OH)_3(s)$ colloidal particles formed under near neutral pH conditions were identified by monitoring the absorbance at around 506-507 nm. The formation of ${Am(Ox)_3}^{3-}$ was detected by red-shifts of the absorption and luminescence spectra of 4 and 5 nm, respectively. In addition, considerable enhancements of the luminescence intensities were observed. The luminescence lifetime of ${Am(Ox)_3}^{3-}$ increased from 23 to 56 ns, which indicates that approximately six water molecules are replaced by carboxylate ligands in the inner-sphere of the Am(III). These results suggest that ${Am(Ox)_3}^{3-}$ is formed through the bidentate coordination of the oxalate ligands.

Perceptions and Demands Analysis on the Creation and Management of School Forests (명상숲 조성·운영에 관한 인식 및 요구도 분석)

  • Jeong, Seongyeop;Lee, Yeonhee
    • Journal of Korean Society of Forest Science
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    • v.108 no.2
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    • pp.259-268
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    • 2019
  • The aim of this study was to investigate whether the schools and local governments were aware of the creation and operation of school forests. A survey was conducted of 45 teachers of schools selected as having the best practices in the school forest and 153 local government officials within the district where the school forests were built. The school teachers attributed the necessity to create school forests to the importance of beautifying the environment. The results showed that the area in which the school forest was created, the school environment after the school forest was created, and use of the school forest were generally satisfactory, but maintenance of the school forest was not somewhat unsatisfactory. Additionally, the results indicated that continuous maintenance was necessary for the effective use of the school forest, and there was a great need for professional personnel to conduct the maintenance program. Furthermore, local government officials recognized that beautification of the school environment was the best effect of creating the school forests. They also realized a great need for local government's support of a maintenance program and its cooperation with related ministries and agencies to create and operate the school forest. Thus, it is crucial to devise measures for maintenance and to establish a system by which schools and local governments can work closely to promote the creation and operation of these school forests.

Diversity and Interaction of Pollination Network from Agricultural Ecosystems during Summer (경북과 강원지역 농업생태계에서 여름철 화분매개네트워크 다양성과 상호작용)

  • Son, Minwoong;Jung, Seongmin;Jung, Chuleui
    • Journal of Apiculture
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    • v.34 no.3
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    • pp.197-206
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    • 2019
  • Pollination is an important ecosystem service involved in plant breeding and reproduction. This study analyzed the pollination network, which is the interaction between flowering plants and flower-visiting insects in the agricultural landscape. Flower-visiting insects from blossoms of flowering crops and surrounding plants were quantitatively surveyed during summer time. The pollinator species and abundance on each flowering plant were analyzed. A total of 2,381 interactions were indentified with 154 pollinators on 30 species of plants. Species richness of the pollinators was highest in Coleoptera (34%) followed by Hymenoptera (28%), Diptera (28%) and Lepidoptera (10%). Apis mellifera dominated (50%) followed by Calliphora vomitoria (5.3%) and Xylocopa appendiculata among pollinators, and remaining wild pollinators provided complex interaction. Among plants, Platycodon grandiflorum, Perilla frutescen and Fagopyrum esculentum harbored most pollinators and showed highest interaction frequencies. In the modular analysis, Apis mellifera was located as a hub-species which connect the interaction of others, implying most important role in the network. This results provide the basic information on the pollinator species associated with each crop and pollinator habitat in which plant provide the nectar, pollen and habitat resources for wild pollinators.

Investigation of microbial contamination in meal kit products purchased via online shopping (온라인에서 구매한 밀키트 제품의 미생물 오염도 조사)

  • Lee, Seung Hun;Nam, Su Jin;Kim, Dong Woo;Kim, Gi Ryeon;Park, Seon Jeong;Lee, Eun Ji;Je, Hyeon Ji;Koo, Ok Kyung
    • Korean Journal of Food Science and Technology
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    • v.54 no.2
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    • pp.235-240
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    • 2022
  • In this study, the delivery conditions and microbial contamination in Mille-feuille Nabe and fresh spring roll meal kits purchased online were assessed. The average surface temperature on the product arrival was 14.4℃. The average number of total aerobic bacteria and coliforms in Mille-feuille Nabe was 3.27 log CFU/g and 1.27 log CFU/g, respectively, in meat, and 6.66 log CFU/g and 2.94 log CFU/g, respectively, in vegetables. The average number of total aerobic bacteria and coliforms in fresh spring rolls was 3.82 log CFU/g and 1.93 log CFU/g in meat, and 5.62 log CFU/g and 3.31 log CFU/g in vegetables, respectively. Escherichia coli was detected in bok choy and perilla leaves, with an average of 0.86 log CFU/g. Salmonella spp. and E. coli O157:H7 were not detected in any of the samples; however, Listeria monocytogenes was detected in all three beef samples. Therefore, before consuming meal kits, sufficient washing and heating are recommended to prevent occurrences of food poisoning.

Changes in Leaf and Reproductive Traits of Mountain Ash (Sorbus alnifolia) as Urban Flourisher in the Seoul Metropolitan, South Korea (한국 서울 식생의 번성자로서 팥배나무의 형질 변화 양상)

  • Jung, Song-Hie;Cho, Yong-Chan;Lee, Chang-Seok
    • Korean Journal of Environment and Ecology
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    • v.35 no.6
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    • pp.644-658
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    • 2021
  • Understanding the functional traits of dominant species in urban ecosystems provides insight into species' trait adaptation and ecosystem function in response to fragmented and isolated urban vegetation and reduced biological interactions. This study compared means and variances of environmental factors (geographic, meteorological, and soil attributes) and 4 leaf traits (leaf area, specific leaf area, leaf dry mass content, and leaf shape index) and 7 reproductive traits (fruit width, fruit length, fruit shape, fruit dry weight, fruit dry matter content, seed weight, and seed ratio) measured of 40 Sorbus alnifolia individuals in four mountainous areas south of Seoul downtown, South Korea. We then performed the multivariate analysis of trait combinations. While the measured environmental factors indicated the individuality of the survey sites, the urban vegetation was drier and had a longer growth period. The leaves of S. alnifolia in the urban areas were smaller and heavier, and the fruits produced longer and lighter seeds, showing the traits affected by long urbanization. The study confirmed changes in the growth and reproduction mechanism of the S. alnifolia population under the urban environment, indicating reduced biological interaction due to vegetation fragmentation and isolation. This study provides limited but distinct ecological information about the function and persistence of key species in cities with a reduced scale of biological interactions and many negative environmental factors such as air pollution.

Study on User Characteristics based on Conversation Analysis between Social Robots and Older Adults: With a focus on phenomenological research and cluster analysis (소셜 로봇과 노년층 사용자 간 대화 분석 기반의 사용자 특성 연구: 현상학적 분석 방법론과 군집 분석을 중심으로)

  • Na-Rae Choi;Do-Hyung Park
    • Journal of Intelligence and Information Systems
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    • v.29 no.3
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    • pp.211-227
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    • 2023
  • Personal service robots, a type of social robot that has emerged with the aging population and technological advancements, are undergoing a transformation centered around technologies that can extend independent living for older adults in their homes. For older adults to accept and use social robot innovations in their daily lives on a long-term basis, it is crucial to have a deeper understanding of user perspectives, contexts, and emotions. This research aims to comprehensively understand older adults by utilizing a mixed-method approach that integrates quantitative and qualitative data. Specifically, we employ the Van Kaam phenomenological methodology to group conversations into nine categories based on emotional cues and conversation participants as key variables, using voice conversation records between older adults and social robots. We then personalize the conversations based on frequency and weight, allowing for user segmentation. Additionally, we conduct profiling analysis using demographic data and health indicators obtained from pre-survey questionnaires. Furthermore, based on the analysis of conversations, we perform K-means cluster analysis to classify older adults into three groups and examine their respective characteristics. The proposed model in this study is expected to contribute to the growth of businesses related to understanding users and deriving insights by providing a methodology for segmenting older adult s, which is essential for the future provision of social robots with caregiving functions in everyday life.