• The Korean Journal of Pesticide Science
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• v.13 no.3
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• pp.185-189
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• 2009

A monoterpenoid compound, benzylideneacetone (BZA), is a metabolite of an entomopathogenic bacterium, Xenorhabdus nematophila. Its primary biological activity is an inhibitor of phospholipase $A_2$, which catalyzes the committed step of biosynthesis of various eicosanoids that are critically important to mediate insect immune responses. When BZA was applied to two-spotted spider mite, Tetranychus urticae, it exhibited a dose-dependent mortality in leaf-disc assay. Subsequently BZA was tested against T. urticae infesting apples in a field orchard, in which it showed a significant control efficacy, which was not statistically different with that of a commercial acaricide. BZA also had significant antibacterial activities against three species of plant pathogenic bacteria when it was added to the bacterial cultures, in which it showed the highest inhibitory activity against a bacterial wilt-causing pathogen, Ralstonia solanacearum. The bacterial pathogen caused significant disease symptom to young potato plants. However, BZA significantly suppressed the disease occurrence. This study suggests that BZA can be used to develop a novel crop protectant to control mite and bacterial pathogen.

• The Bimonthly Magazine for Agrochemicals and Plant Protection
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• v.8 no.3
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• pp.110-123
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• 1987

• The Bimonthly Magazine for Agrochemicals and Plant Protection
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• v.13 no.5 s.110
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• pp.88-93
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• 1992

• Korean journal of applied entomology
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• v.36 no.1
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• pp.30-36
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• 1997

Except for a cosmopolitan and major pest of apples, Tetranychus urticae Koch, Tetranychus mites in Korea such as T. viennensis Zaher, T. kanzawai Kishida, and T. truncams Ehara have been considered as quarantine pests by Canada and United States. Even though these mites are not feeders on apples, they are suspected to attach accidentally on apple h i t s in autumn as females enter the diapause. The characters used to identify Tetranychus mites have been confined to the shape of aedeagus in adult male. To develope a fast and accurate alternative identification protocol applied to hibernating female mites on apples, their mitochondrial DNA (mtDNA) were examined to find out any polymorphisms to discriminate each species from the other ones. Three pairs of primers for polymerase chain reaction (PCR) were used to amplify cytochrome oxidase subunit I (CO-I) coding region in mitochondrial DNA5 of four species of Tetranycus mites. The longest amplified product was estimated its size as about 680 bp. Digestion with restriction enzymes, AluI, Ddel, and Sau3A, showed length polymorphisms, which will he useful as diagnostic markers to identify Tetranychus mites. Schematic restriction maps in amplified region were shown for each species.

• The Korean Journal of Pesticide Science
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• v.15 no.3
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• pp.317-322
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• 2011

A comparative experiment was conducted to investigate the persistence of chlorfenapyr residue and its biological activity to two spotted spider mite, Tetranychus urticae, after treatment of chlorfenapyr suspension concentrate (10% SC) on paprika plants at recommended (2000 times dilution) and double dosage. Biological half-lives of chlorpfenapyr were estimated as 8 and 5 days in leaves and fruits of paprika plants, respectively. While initial deposits of chlorfenapyr residues in leaves at recommended and double dosages were 22.22 and 37.75 ${\mu}g\;g^{-1}$ at the time of application, its residue decreased to 1.56 ${\mu}g\;g^{-1}$ and 3.62 ${\mu}g\;g^{-1}$ at 29 days after treatment, respectively. Residual biological activity of chlorfenapyr SC to T. urticae at recommended dosage lasted for 7 or 15 days on the basis of 24 or 48-hrs mortality test assayed with feeding on excised leaf chlorfenapyr-treated. The control efficacy to the mite showed a good agreement with the persistence of chlorfenapyr residues in leaves.

• Korean journal of applied entomology
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• v.37 no.1
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• pp.53-58
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• 1998

Comparative studies on some ecological characteristics of three phytoseiid mitespecies (one native; Arnblyseius womersleyi Schica, and two introduced species; A. fallacis Garmanand Typhlodromus occidentalis Nesbit) were carried out. The two-spotted spider mite (Tetranychusurticae Koch) was supplied as prey. Under four constant temperatures of 20, 23, 25 and 30f OS$^{\circ}$C,duration of growth from egg to adult for A. womer.vleyi was 11.5, 7.7, 6.7 and 5.6 days. While twoother species needed slightly shorter time but not significantly different. Critical temperature andeffective degree-days (DD) of A. womerslevi females were 83$^{\circ}$C and 1 1 1.6 DD, whereas those of A..fallacis were 10.7"C and 86.0 DD, and those of 7: occidentalis were 10.7"C and 94.1 DD. Also, thoseof males were similar to their females. Average longevity of females of A. womersleyi, A. fallucisand 7: occidentalis were 18.2 k 8.67, 19.6 3~7.18 and 13.0f5.66 days, total fecundity were 34.3 $-11.93, 39.8k 12.64 and 23.6k8.86, respectively. Under four constant temperatures of 20, 23, 25 and30-t0.S$^{\circ}$C, A. womersleyi consumed 9.1 f2.49, 9.7 k2.00, 9.7 f 2.61, and 10.3 k2.33 eggs of 7:urticae throughout their development. A. ,fizlluc~i.sc onsumed 10.2 k 2.52, 9.7 f2.29, 10.7 f 2.37 and10.1 k2.62 eggs, while, 7: occidentalis consumed 1 1.9 k3.43, 14.2 f4.50, 14.8 k 3.2 1 and 12.7 f2.95 eggs, respectively. Gravid females of A. womersleyi, A. f~zllacis and 7: occidentalis consumed11.4f1.59, 12.5k1.43 and 11.7k3.07 eggs, or consumed 11.9f 2.63, 12.4k3.82, and 12.5f 3.73protonymphs of 7: urticae in a day at 25-30$^{\circ}$C.e in a day at 25-30$^{\circ}$C.

• Proceedings of the Korean Society of Applied Entomolohy Conference
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• 2000.10a
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• pp.62-62
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• 2000

• Proceedings of the Korean Society of Applied Entomolohy Conference
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• 2002.11a
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• pp.165-165
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• 2002

• Proceedings of the Korean Society of Applied Entomolohy Conference
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• 2000.05a
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• pp.119-119
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• 2000

• The Korean Journal of Soil Zoology
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• v.9 no.1_2
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• pp.1-5
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• 2004

Insect pests and their natural enemies of Hibiscus Linne (Malvaceae) were investigated from March 2002 to November 2004. Fourteen insect pest species of 9 families in 5 orders were collected from Hibiscus syriacus: 5 species in Homoptera, 3 species in Lepidoptera, 2 species in Coleoptera, 1 species in Orthoprera, 1species in Hemiptera, 1 spedies in Acarina, and 1 species in Stylommatophora. Especially, Aphis gossypii Glover (Aphididae), Anomis megogona Walker(Noctuidae) and Tetranychus urticae Koch (Tetranychidae) were very important species because of their increasing daminge. The highest donsities were observed from May to June in August in Tetranychus urticae. As the enemies and ento-mopathogens of insect pests on Hibiscus syriacus, 1 species of bacteria, 3 species of fungi, 1 species of fungi, 1 species of Hemiptera, 1 species of Coleoptera, 2 species of Hymenopetera, 2 species of Diptera, and 1 species of Acarina were investigated. As the predators and parasitoids of Aphis gossypii, Aphidoletes aphidoletes aphidimyza Rondani (Cecidomyiidae), Meliscaeva cinctella Zetterstedt (Syrphidae), Harmonia axyridis Pallas (Coccinellidae), and Aphidius gifuensis Ashmead (Braconidae), entomopathogenic fungi, Vericillium lecani naturalis strain (Moniliaceae) and Beauveria bassiana naturalis strain strain (Hypocreaceae) were observed and Bacillus thuringiensis naturalis strain (Bacillaceae), B. bassiana, Metarhizium anisopliae naturalis strain (Hypocreaceae), predators of Tetranychus urticae, Amblyseius sp. (Phytoseiidae), and Orius sp. (Anthocoridae) were observed.