• Horticultural Science & Technology
• /
• v.32 no.1
• /
• pp.60-65
• /
• 2014

This study was conducted to investigate the antimicrobial effect of chlorine dioxide ($ClO_2$) on the vase life of cut rose 'Beast' (Rosa hybrida L.). Postharvest treatments to extend the vase life of cut roses were divided into two: holding solution treatment and pulsing solution treatment. In holding solution treatment, the cut roses were treated with preservative solutions containing tap water (TW, control), distilled water (DW), $ClO_2$ 2, 4, 6, and $8{\mu}L{\cdot}L^{-1}$, and compared with a commercialized antimicrobial compound of 8-HQS $200{\mu}L{\cdot}L^{-1}$. In pulsing solution treatment, cut roses were dipped into the $ClO_2$ solutions of 50, 100, 150, 200, and $250{\mu}L{\cdot}L^{-1}$ for 60 seconds and were placed in DW. The air temperature was $18.4^{\circ}C$, RH 51.5%, and light (photosynthetically active radiation, PAR) $3.6{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ with 12 hour day length. The longest vase life of cut roses was observed in the holding solution with $ClO_2$ $4{\mu}L{\cdot}L^{-1}$ as 13.8 days and pulsing with $200-250{\mu}L{\cdot}L^{-1}$ as 13.5-13.7 days, where vase life were extended four days longer than TW. Whereas, the inclusion of 8-HQS $200{\mu}L{\cdot}L^{-1}$ in vase solution resulted in phytotoxicity. The relative fresh weight and water uptake have similar tendencies. Bacteria inhibition by $ClO_2$ and 8-HQS were very effective. But bacteria at TW and DW treatments on cut flower with stem were detected in $3.7{\times}10^5CFU{\cdot}L^{-1}$ and $6.3{\times}10^5CFU{\cdot}L^{-1}$, respectively (without stem in DW $1.4{\times}10^4CFU{\cdot}L^{-1}$). The $ClO_2$ contents in holding solution of all treatments were scavenged in two-four days after treatment. This study indicated that $ClO_2$ $4{\mu}L{\cdot}L^{-1}$ holding solution treatment and $200-250{\mu}L{\cdot}L^{-1}$ pulsing solution treatment can be applied to extend the postharvest life of cut roses.

• FLOWER RESEARCH JOURNAL
• /
• v.19 no.3
• /
• pp.151-157
• /
• 2011

This research was aimed to extend the vase life and improve the quality of cut roses 'Marina'. This study investigated the effect of three different angles and sucrose concentration on the vase life and the quality of cut rose flowers. In first experiment, cut roses were placed in three different angles ($0^{\circ}$, $45^{\circ}$ and $90^{\circ}$) in holding solutions containing 0.5, 1 and 2% sucrose (soaking treatment), and examined the number of petals of cut roses. In second experiment, cut roses were placed in three different angles after pre-treated in 0, 1, 2 and 4% sucrose solution for 2 and 20 hours, and examined the flower diameter of cut roses. In third experiment, cut roses were examined the pigmentation, flowing response and chlorophyll contents in cut roses by three different angles after pulsing treatment (pre-treatment) in holding solution containing 2% sucrose for 2 and 20 hours. Results of this study showed that cut roses were influenced by arranging angles and sucrose concentrations. The vase life of cut roses were extended in $45^{\circ}$ and $90^{\circ}$ arranging angle, as compared with the control (distilled water), and the holding solution containing 2% sucrose significantly increased vase life of cut roses(P < 0.05). Flower diameter of cut roses pre-treated in 4% sucrose solution for 2 and 20 hours significantly decreased (P < 0.05). The pigmentation and flowing response in cut roses were beneficial in $45^{\circ}$ arranging angle treated with 2% sucrose solution for 2 and 20 hours. Chlorophyll content in leaves of cut roses was not influenced by arranging angles. The pigmentation and flowing response of $0^{\circ}$ and $45^{\circ}$ arranging angles were better than that of 90 arranging angle. The vase life of cut roses in $45^{\circ}$ arranging angle was more prolonged than that in $90^{\circ}$ arranging angle. In conclusion, the arranging angles seem to be effective in retarding senescence of cut rose flowers.

• Horticultural Science & Technology
• /
• v.33 no.1
• /
• pp.61-69
• /
• 2015

This study was carried out to investigate the effects of shipping temperature and harvesting stage on the quality and vase life of cut flower in standard chrysanthemum 'Baekma' for export. Cut flowers harvested at flowering stage 4 were stored for 24 hours at $5^{\circ}C$, and then quality and vase life were evaluated after simulated shipping for 48 hours at 5, 20, and $35^{\circ}C$. In addition, cut flowers harvested in flowering stage 1 to stage 6 were evaluated after simulated shipping for 72 hours at $5^{\circ}C$. As shipping temperature increased, $CO_2$ concentration inside the box rapidly increased, and $O_2$ concentration greatly decreased. The vase life of cut flowers was extended by 3 days and chlorophyll content of leaves, fresh weight, solution uptake, and flower diameter were better maintained in $5^{\circ}C$ shipping than in 20 or $35^{\circ}C$ shipping. The vase life of cut flowers harvested at flowering stage 1 or 2 was extended by 5.2 or 5.5 days compared to those harvested at flowering stage 6, more 6. The fresh weight was lower and flower diameter was smaller by 1.3 or 2.5 cm in cut flowers of flowering stages 1 through 3 than flowering stages 4 through 6. In addition, the cut flowers of flowering stages 4 through 6 showed higher solution uptake than those of flowering stages 1 through 3. These results suggest that shipping at $5^{\circ}C$ of cut flowers harvested at flowering stage 4 is preferential for promoting vase life and quality of cut flower in standard chrysanthemum 'Baekma' for export to Japan.

• FLOWER RESEARCH JOURNAL
• /
• v.19 no.1
• /
• pp.42-48
• /
• 2011

This experiment was conducted to improve postharvest quality and extended vase life of Korean native Aster koraiensis Nakai for use as cut flowers. Aster koraiensis Nakai cut flowers were treated with various pretreatment and holding solution. Postharvest pretreatment with 3% sucrose + $200mg{\cdot}L^{-1}$ HQC + $50mg{\cdot}L^{-1}\;AgNO_3$ + $50mg{\cdot}L^{-1}$ BA and 3% sucrose + $200mg{\cdot}L^{-1}$ HQC + $50mg{\cdot}L^{-1}\;AgNO_3$ + $150mg{\cdot}L^{-1}$ citric acid for 16 hours extended vase life of cut Aster koraiensis Nakai flowers by 1.4 times as compared with the control (distilled water). Holding solution of 3% sucrose + $200mg{\cdot}L^{-1}$ HQC + $50mg{\cdot}L^{-1}\;AgNO_3$ + $50mg{\cdot}L^{-1}$ BA and 3% sucrose + $200mg{\cdot}L^{-1}$ HQC + $50mg{\cdot}L^{-1}\;AgNO_3$ + $150mg{\cdot}L^{-1}\;GA_3$ extended vase life of cut Aster koraiensis Nakai flowers by1.6 and 1.7 times as compared with control (distilled water). Aster koraiensis Nakai.flowers held in this preservative solution increased fresh weight and were maintained positive water balance for a long vase period. It was suggested that the vase life of cut Aster koraiensis Nakai flowers was closely related to fresh weight and water balance of the cut flower.

• Horticultural Science & Technology
• /
• v.28 no.4
• /
• pp.609-617
• /
• 2010

The effect of vase water temperature and leaf number on water relations and senescence responses was determined in cut roses. Freshly harvested 'Red Sandra' roses were re-trimmed to 50 cm leaving two or four upper leaves and held in one of three solutions: ambient temperature distilled water ($23^{\circ}C$; AT-DW), low temperature distilled water ($7^{\circ}C$; LT-DW) and low temperature preservative solution (LT-PW). Flowers were kept in an environmental controlled room. Treatment effects evaluated were vase life, flower diameter, and changes in fresh weight and water uptake. Differences in water relations were determined by measuring $CO_2$ assimilation, stomatal conductance, and stem water flux rate (SFR). The water uptake rate was significantly increased in roses in LT-DW and decreased in those in LT-PW. While showing lower solution uptake rate during vase period, roses in LT-PW exhibited greatest fresh weight, longest positive water balance duration and largest flower diameter. Flowers with two leaves attached exhibited a higher fresh weight and improved water balance, thereby extending vase life. $CO_2$ assimilation rate and stomatal conductance were significantly decreased by placing flowers in LT-PW, yet increased by reducing leaf number to two leaves on the flower stems. Compared to the upper stem, the SFR of the basal stem of roses in AT-DW was lower, whereas SFR in basal stems of roses in LT-DW was much higher, suggesting that low-temperature water improved the hydraulic conductance in the stems. In contrast, roses in LT-PW had a stable SFR during the experimental period and displayed a similar pattern in SFR between upper and basal portions of the stems. Consequently, the vase life of cut roses in LT-PW and LT-DW was extended by more than eight and four days, respectively, compared to those in AT-DW.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2018.10a
• /
• pp.51-51
• /
• 2018

본 연구는 바이오에너지용 이질3배체 억새(Miscanthus ${\times}$ giganteus) 품종육성 위한 교배재료인 물억새(M. sacchariflrous)와 참억새(M. sinensis) 출수기 단축과 단교배 방법을 개발하기 위해 수행하였다. 물억새와 참억새를 12시간 일장, 자연일장 조건에서 재배하여 출수기 단축에 미치는 단일효과를 조사하였다. 출수기에 일중 화분 발아시간, 절단한 화분친 이삭 활력 유지 및 격리방법 등 단교배 방법을 구명하여 이를 활용한 억새의 자가 수정 여부를 조사하고 시험교배를 실시하였다. 참억새와 물억새 모두 12시간 일장의 단일 조건에서 재배한 것이 자연일장에 재배한 것에 비해 출수 소요일수가 18~27일 정도 단축되었다. 화분 발아는 물억새에서 오전 6시에 왕성하였으나 시간이 경과할수록 발아율이 낮아져 오전 8시에는 10% 이하만 발아하였다. 참억새 화분은 오전 6시에는 발아하지 않았으나 오전 7시에 50% 이상이 발아하였고 8시에는 물억새와 같이 10% 이하였다. 화분친 참억새 이삭을 절단, 절화 보존액에 꽂아 백색 부직포로 격리하였을 때 절화 보존액량이 많을수록 활력 유지 일수가 증가하여 150 mL에서 물억새, 참억새 모두 7일간 개화 및 화분 비산을 지속하였다. 이 때 화분 발아율은 참억새와 물억새 모두 4일까지 40%를 유지하였다. 참억새와 물억새는 자가수정율이 ~ 2.5%로 낮고, 자연교잡 임실율은 출수기가 빠른 유전자원에서 54.4%까지 높았다. 상기 단교잡 방법을 적용한 물억새 4배체와 참억새 2배체간 14조합 시험교배로 437립의 종자를 얻었다. 본 연구의 억새 종간 단교배 방법은 우수한 종자친과 화분친으로 교배종자를 얻을 수 있어 향후 바이오매스 수량이 많으면서, 종자가 맺히지 않은 이질 3배체 품종육성에 활용할 수 있을 것으로 판단된다.

• Journal of Life Science
• /
• v.10 no.6
• /
• pp.591-597
• /
• 2000

An investigation was conducted to elucidate the effect of sucrose, germicides and abscisic acid (ABA) in the preservatives on postharvest quality, such as fresh weight, solution uptake, vase life, diameter and thickness of flower, and chlorolhyll of leaf in cut chrysanthemum 'Kyoungsubang`. Fresh weight of cut chrysanthemum was highest in 250mg/L aluminum sulfate [{TEX}$Al_{2}(SO_{4})_{3}${/TEX}]+3% sucrose and 250 mg/L 8-hydroxyquinoline sulfate (8-HQS)+ 3% sucrose. Addition of sucrose and germicides inhibited microorganisms growth in solution, and apparently promoted solution uptake. The increased fresh weight seems to attribute to the elevated uptake. Germicide 8-HQS was more effective than {TEX}$Al_{2}(SO_{4})_{3}${/TEX} in the aspect of solution uptake and fresh weight. Treatment of 250 mg/L 8-HQS +3% sucrose showed the longest vase life (24.67±2.52 days) and the lowest rate of leaf chlorosis (or senescence). Addition of {TEX}$Al_{2}(SO_{4})_{3}${/TEX} and sucrose accelerated leaf chlorosis and increased diameter of flower, respectively. Sucrose treatment prolonged the vase life of flowers. Except control and 250 mg/L 8-HQS, content of chlorophyll was rapidly decreased after 6 days at upper, middle and lower leaves in order.

• Korean Journal of Plant Resources
• /
• v.32 no.5
• /
• pp.509-518
• /
• 2019

This study was conducted to investigate single crossing condition of M. sacchariflrous and M. sinensis for breeding of M. ${\times}$ giganteus cultivar. Compared with natural day length condition, cultivation in short day length condition shorten days to heading to 18~27 days in both species. Pollen germination ratio of were 75.8% at 6 o'clock in M. sacchariflorus and 51.9% at 7 o'clock in M. sinensis but decreased to below 10% at 8 o'clock in both species. When cut ears immerged in 150 mL of cut-flowers conservation solution and isolated with covering of white non-woven fabric, flowering and pollen dispersal were persisted for 7 days, and the ratio of pollen germination were above 40% for 4 days. The ratio of self-fertilization of both species were below 2.5%, but open pollenation ratio were above 50%. We obtained 437 seeds with experimental single cross of 14 combinations between tetraploid M. sacchariflorus and diploid M. siensis by application of developed single crossing methods. In the single cross, numbers of seed set were different by mother plants. Thus, the newly investigated single crossing condition will be used to breed M. ${\times}$ giganteous cultivar which is sterile and has superior characteristics of biomass yield.