• KSBB Journal
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• v.16 no.5
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• pp.500-504
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• 2001

We scaled up a covalent immobilization system of urokinase to the activated Sepharose and used it repeatedly to cleava a fusion protein consisting of human growth hormone and GST fragment. After scale up from 6 ml to 250 ml. the column system still demonstrated basically the same performance in terms of urokinase immobilization and fusion protein cleavage. When the column was washed with 6 M guanidine HCI after the cleavage reaction, the immobilized urokinase showed no activity probably becasue it was fully unfoled. However, as the denaturant was gradually removed from the column the immobilized urokinase fully regained its bioactivity, which indicated it was properly refolded into is natie conformation as covalently attached to the solid matrix. After 20 cycles of this solid-phase unfolding/refolding. the immobilized urokinase maintained approx. 80% of the initial bioactivity. This method provides and efficient protocol to apply the solid-phase refolding technique to improve the longevity of immobilized enzyme columns.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.20 no.3
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• pp.247-254
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• 2008

The double-peak normal distribution function (DPDF) suggested by Cho et al.(2004) has the problems that the extremely high and low tidal elevations are frequently generated in the Monte-Carlo simulation processes because the upper and lower limits of the DPDF are unbounded in spite of the excellent goodness-offit results. In this study, the modified DPDF is suggested by introducing the upper and lower value parameters and re-scale parameters in order to remove these problems. These new parameters of the DPDF are optimally estimated by the non-linear optimization problem solver using the Levenberg-Marquardt scheme. This modified DPDF can remove completely the unrealistically generated tidal levations and give a slightly better fit than the existing DRDF. Based on the DPDF's characteristic power, the over- and under estimation problems of the design factors are also automatically intercepted, too.

• Journal of Information Management
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• v.23 no.1
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• pp.39-62
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• 1992

In this paper about 60 auxiliary words and 320 stopwords are selected from analysis of sample data, four types of stop word are classified left, right and - auxiliary word truncation & normal. And a keyword extraction system is suggested which undertakes efficient truncation of auxiliary word from words, conversion of Chinese word to Korean and exclusion of stopword. The selected keyeords in this system show 92.2% of accordance ratio compared with manually selected keywords by expert. And then compound words consist of $4{\sim}6$ character generate twice of additional new words and 58.8% words of those are useful as keyword.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.37 no.1
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• pp.77-82
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• 2013

A cyclone separator has been widely used in various industrial processes for removing fine particulate matter because it is easy to fabricate, cost effective, and adaptable to extremely harsh conditions. However, owing to the complex flow field in cyclones, a complete understanding of the detailed mechanisms of particulate removal has not yet been gained. In this study, a theoretical analysis was performed for calculating the collection efficiency and cut-off size in cyclones by taking into account the effects of geometrical and flow parameters. The collection efficiency and cut-off size values predicted by the theoretical model showed good agreement with experimental measurements for particles with a diameter of $0.5-30{\mu}m$. It was also revealed that the surface friction, along with the flow and geometrical parameters, has a significant effect on the cyclone performance.

• Korean Journal of Plant Tissue Culture
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• v.28 no.2
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• pp.103-108
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• 2001

The in vitro plantlets of cassava (Manihot esculenta Crantz cv. MColl 22) could be regenerated from nodal explant cultures in a liquid MS basal medium containing 0.01 mg/L zeatin for 2 weeks. The plantlets of 1.5∼2.5 cm in shoot length were transplanted to a glass bottle containing fine sand and acclimated under non-sterile conditions after excising their intact roots by: 1) prune leaving roots base of 1∼1.5 cm; 2) complete removal of roots; and 3) cutting off the rooting zone. The majority of in vitro-formed intact roots continued growth after transferred to soil, and all of the damaged roots stopped further growth. The plantlets with excised roots began to develop new roots within 7∼10 days after being transferred to a glass bottle, and a few of the pruned roots developed lateral roots from the remaining portion. Pruning and removal of in vitro roots resulted in a high survival rate (>87%), and did not significantly affect ex vitro root regeneration and acclimation, but the plantlets in which the rooting zone had been cut-off showed 73% survival rate. Pruning or removal of in vitro roots before transfer of plantlets is recommended for useful method of commercial micropropagation because of easier handling and high survival rate of plantlets.

• Food Science and Preservation
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• v.6 no.4
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• pp.351-356
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• 1999

To develop and extend the shelf-life of vitamin C enriched fresh-cut apples using CA stored Fuji apples, dipping in 10% L-ascorbic acid and packaging with 0.03 mm LDPE, 0.04 mm PP and 0.08 mm Nylon/PE film(N$_2$displacement) were carried out. The changes of gas concentrations in the packaging and quality attributes of fresh-cut apples were examined during storage at 10$^{\circ}C$. The concentrations of O$_2$was maintained lower in Nylon/PE film than the other film, the level of O$_2$was in the range of 1∼3%. The increase of C$_2$H$_4$ concentrations in Nylon/PE film bag was more suppressed than the others. The vitamin C content of fresh-cut apples was enriched by dipping in L-ascorbic acid solution up to 241 mg$.$100 g-1 f.w., and the loss or that content was retarded differently by the package conditions of lower O$_2$level during storage. Browning in fresh-cut apples was shown after 6 days of storage in LDPE and PP film. but it was not shown by 14 days of storage in Nylon/PE film. Spoilage and off odor in fresh-cut apples were not detected up to 14 days of storage in Nylon/PE film. The results indicated that the vitamin C enriched fresh-cut apples can be processed from the long-term CA stored apples, and maintaining high quality of the products ill be possible in cases of the application of sealing packaging after O$_2$removal with film having low O$_2$permeability.

• Journal of the Korean Society for Precision Engineering
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• v.2 no.2
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• pp.6-10
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• 1985

최근, 절삭가공 기술의 향상으로 공작기계의 자동화, 수치제어화가 되어 감에 따라 절삭가공 할때 생성되는 chip을 이용하게 처리할 수 있는 방법을 강구하는 것이 중요한 문제점으로 부각되고 있다. 절삭기계공장의 생산능률 및 합리화를 추진함에는 Chip의 원활한 처리법이 병행되지 않고는 단순히 공작기계의 개발, 개량만으로 해결되지 않는다고 본다. 절삭가공할 때 생성된 긴 chip이 공구와 공작물등에 감겨서 기물을 손상시킬 뿐만 아니라 Chip의 열로 인하여 공작물 또는 기계가 열변형을 받게 되는 경우가 있으므로 작업을 중지시켜서 작업원이 Chip 을 제거해야 할 번거러움 이 있다. 그러므로 chip 처리에 대한 연구보고는 Henriksen시대에서부터 시작하여 현재까지 많은 보고가 있다. 일반적으로 연속형 chip을 세단하는 방법으로는 불연속형 Chip법, 단속절삭법, 자동 절단촉진법 및 절단공구법으로 구분된다.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.11 no.1
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• pp.33-38
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• 2012

An experimental study was carried out to observe the characteristics of impingement of assist gas from a rectangular supersonic nozzle on kerf surface in laser machining. A micro-scale supersonic (Mach number 2.0) rectangular nozzle system was designed and fabricated for the purposes, and hot tests of the performance of the nozzle system was proceeded in the ITI corporation laboratory. For various related parameters such as laser powers, nozzle pressures and cutting speeds, the quality of the frontal view of cut edge surfaces was observed by a microscope. In the study, it was shown that the application of the present micro-rectangular supersonic nozzle in an off-axis configuration made it possible to cut a mild steel, by combinations of relatively low laser - powers, large standoff distances, and assist gas with no oxygen, which was not achieved by conventional laser cutting processes.

• 기계와재료
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• v.2 no.3 s.5
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• pp.120-127
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• 1990

미래지향적 고부가가치의 첨단산업 전반에 걸쳐 기계요소 부품 재료로서 주목받고 있는 화인 세라믹스의 가공에 있어 다이아몬드 연삭 숫돌에 의한 연삭, 절단 가공은 가장 중요한 위치를 차지하고 있는 기계적 제거 가공법이다. 그러나 세라믹스는 고경도, 고강도, 경취성 재료이기 때문에 다이아몬드 지립의 절인 마모가 빨라 능률적인 가공을 장시간 유지하기가 곤란하다. 여기서는 국내 기계 가공 작업 현장에 있어서 부품의 신소재 대체에 따라 필연적으로 사용하게 된 다이아몬드 연삭 숫돌에 대하여 작업자들이 범용 숫돌 사용시의 고정 관념만을 갖고 새로운 공구에 관한 기초 지식이나 Know-how없이 가공작업에 임하는 경우가 많아, 압력 절입 방식에 있어서의 연삭 능률 비교를 중심으로 평면 연삭이나 절단 등의 강제 절입 방식에 있어서의 연삭비, 연삭저항 등의 데이터도 첨부하여 세라믹스 가공을 위한 다이아몬드 연삭공구의 조건에 대해 논하고자 한다.

• Proceedings of the Korean Institute of Resources Recycling Conference
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• 2004.05a
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• pp.186-190
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• 2004

실리콘 잉곳의 절단공정에서 발생하는 폐슬러지는 실리콘과 실리콘카바이드 등의 유가자원이 함유되어 있으며, 이를 효과적으로 분리, 회수하는 방법에 대해 검토하였다. 폐슬러지에 함유된 오일은 유기 용매에 의해 용해되어 효과적으로 분리되었고, 불순물인 철분은 자력선별에 의해 제거할 수가 있었다. 또한 실리콘과 실리콘카바이드의 혼합 분말은 중액선별을 통하여 고순도의 실리콘과 실리콘카바이드로 분리할 수가 있었다.