• Microbiology and Biotechnology Letters
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• v.26 no.2
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• pp.122-129
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• 1998

It were reported that antifungal mechanism of Enterobacter cloacae is a volatile ammonia that produced by the strain in soil, and the production of ammonia is related to the bacterial urease activity. A powerful bacterium SH14 against soil-borne pathogen Fusarium solani, which cause root rot of many important crops, was selected from a ginseng pathogen suppressive soil. The strain SH14 was identified as Bacillus subtilis by cultural, biochemical, morphological method, and $API^{circledR}$ test. From several in vitro tests, the antifungal substance that is produced from B. subtilis SH14 was revealed as heat-stable and low-molecular weight antibiotic substance. In order to construct the multifunctional biocontrol agent, the urease gene of Bacillus pasteurii which can produce pathogenes-suppressive ammonia transferred into antifungal bacterium. First, a partial BamH I digestion fragment of plasmid pBU11 containing the alkalophilic B. pasteurii l1859 urease gene was inserted into the BamH I site of pEB203 and expressed in Escherichia coli JM109. The recombinant plasmid was designated as pGU366. The plasmid pGU366 containing urease gene was introduced into the B. subtilis SH14 with PEG-induced protoplast transformation (PIP) method. The urease gene was very stably expressed in the transformant of B. subtilis SH14. Also, the optimal conditions for transformation were established and the highest transformation frequency was obtained by treatment of lysozyme for 90 min, and then addition of 1.5 ${mu}g$/ml DNA and 40% PEG4000. From the in vitro antifungal test against F. solani, antifungal activity of B. subtilis SH14(pGu366) containing urease gene was much higher than that of the host strain. Genetical development of B. subtilis SH14 by transfer of urease gene can be responsible for enhanced biocontrol efficacy with its antibiotic action.

• Korean Journal of Communication Studies
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• v.22 no.2
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• pp.89-121
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• 2014

In order to suggest a strategy for continuous growth of webtoon, this article examined webtoon usage motivation and tried to make a prediction about culture content products and services connected with webtoon, including intention for viewing movies, based on webtoon; intention for switching to paid webtoon content, and intention for buying webtoon character products. From the point of view of Uses and Gratification Theory intentions for using webtoon and human sociocultural behavior intention are already predicted but with the usefulness of Theory of Planned Behavior Integrated Model this study extended the explanation power of prediction about webtoon related behavioral intention. Results found 5 motivational factors for webtoon usage i.e. 'seeking information', 'entertainment and access availability', 'webtoon genre characteristics', 'influence from a friend or acquaintance', and 'escapism and tension release'. Among them the ones that influenced the intention for viewing movies, based on webtoon, were found to be 'webtoon genre characteristics', 'escapism and tension release' and the 3 variables from Theory of Planned Behavior. 'Seeking information', 'entertainment and access availability', 'webtoon genre characteristics', and all the 3 variables from Theory of Planned Behavior were found to influence the intention for switching to paid webtoon content. The intention for buying webtoon based character products was affected by the motivational factors 'seeking information', 'escapism and tension release' and the behavior and subjective norms variables from Theory of Planned Behavior. Based on the uncommon results from the research several suggestions were made for the continuous growth of webtoon.

• Proceedings of the KSR Conference
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• 2011.10a
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• pp.1681-1698
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• 2011

According to the demands of the times, Inchon international airport railroad opened up step by step but It is situation that much government subsidies are paid because It does not competitive power with road traffic by decline of schedule speed. Inchon international airport railroad users will convert by other transportation and minimize damage their time as travel time is prolonged. Therefore, need high speedization of airport railroad, this may act big role competitive power security as well as airport railroad activation with another public transportation. Thus, this paper suggests that a high speedization possibility section in the sections of airport railroad and train speed elevation effect analysis by roadbed, railroad, system improvement and the most efficient operation intervals analyzing each vehicles (AREX (120km/h), KTX (230km/h), EMU(Electric Multiple Unit, 180km/h) train running pattern.

• Journal of Navigation and Port Research
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• v.38 no.4
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• pp.373-378
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• 2014

In order to prepare for recapitalization of differential GNSS (DGNSS) reference station and integrity monitor (RSIM) due to GNSS diversification, this paper focuses on differential correction algorithm using GPS/Galileo pesudorange. The technical standards on operation and broadcast of DGNSS RSIM are described as operation of differential GPS (DGPS) RSIM for conversion of DGNSS RSIM. Usually, in order to get the differential corrections of GNSS pesudorange, the system must know the real positions of satellites and user. Therefore, for calculating the position of Galileo satellites correctly, using the equation for calculating the SV position in Galileo ICD (Interface Control Document), it estimates the SV position based on Ephemeris data obtained from user receiver, and calculates the clock offset of satellite and user receiver, system time offset between GPS and Galileo, then determines the pseudorange corrections of GPS/Galileo. Based on a platform for performance verification connected with GPS/Galileo integrated signal simulator, it compared the PRC (pseudorange correction) errors of GPS and Galileo, analyzed the position errors of DGPS, DGalileo, and DGPS/DGalileo respectively. The proposed method was evaluated according to PRC errors and position accuracy at the simulation platform. When using the DGPS/DGalileo corrections, this paper could confirm that the results met the performance requirements of the RTCM.

• Information Systems Review
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• v.13 no.3
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• pp.65-81
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• 2011

The purpose of this study is to examine the role of social communication through the information technology, affective commitment and calculative commitment in globally integrated. The structural equation modeling technique was used to evaluate its empirical validity and causal relationship using data collected from a survey by a transnational corporation. The result revealed that social communication between employees through the information technology led to having a direct effect on both affective commitment and calculative commitment beyond the simple communication tool. Also the social communication had a positive effect on globally integrated. Both affective commitment and calculative commitment have a positive effect on globally integrated. From the result, we could find that affective commitment and calculative commitment partially play a mediating role between social communications and globally integrated. This result offered both recommendations and implications for firms attempting to innovate in order to be transnational corporations.

• Korean Journal of Poultry Science
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• v.38 no.4
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• pp.323-330
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• 2011

The chicken monoclonal antibody (mAb), 13C8, reacts with sporozoite antigens of Eimeria spp. which causes avian coccidiosis. Since this mAb was produced at low amount due to genetic instability of chicken hybridoma, a recombinant 13C8 single-chain Fv (ScFv) antibody was constructed by amplification of the variable domain of heavy (VH) and light chain (VL) genes of antibody derived from chicken hybridoma. The constructed 13C8 ScFv was successfully expressed in E. coli and purified as a soluble form. In ELISA analysis, this recombinant 13C8 ScFv antibody showed antigen binding activity as the original mAb. In addition, nucleotide sequence comparison of 13C8 gene to the germline chicken VL and VH genes suggested that the gene conversion with $V{\lambda}$ and VH pseudogenes might contribute to the diversification of VL and VH genes in chickens.

• The Journal of the Korea Contents Association
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• v.5 no.4
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• pp.111-121
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• 2005

The appearance of digital culture swiftly has changed the culture in domestic and international arenas before and after the year 2004 and the image and animation have become two of the most important expression media in contemporary age. Among the Japanese animations that have demonstrated the rapid development of cartoon and animation in the world, the director Dakahata Isao's 'Graves of the Fireflies' that has influenced many works has been evaluated as one of the noticeable works that has a unique method and scenario dramatization in terms of producing the original novel into an animation. This study investigates the metaphor and symbolism shown in this work according to each sequence, divides the production ability in the work into three elements and applies them to the important elements such as camera, colors and mise-en-scene when the original work is depicted into image. It can be summarized in more detail as in the following: firstly, I study the rhythm of camera corresponding to the symbolism of the angle that the camera has and production; secondly, I analyze the artistic elements appeared in the process of expressing the original work into the image, especially the production for the colors and symbolism contained in them and the composition of screen. Thirdly, I analyze how effectively the atmosphere for the situations for the original work is expressed in animation with the aid of one of the image elements, mis-en-scene. It is expected that the analyzed findings will be effective as a way of overcoming the limitation of expressions that the original work in text and the study on these processes will become good examples to the relevant workers and will be the good references to the producers who are interested in the creation of animation in Korea.

• Journal of the Korean association of regional geographers
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• v.22 no.3
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• pp.499-514
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• 2016

Social enterprise is defined as a sort of companies that pursue both publicity and profitability. It is usual that their business activities and viability are dependent upon localized resources in terms of a labor market, raw material procurement and sales market. Also, the characteristics and viability of social enterprises based on local resources within the framework of social economy policy. The social economic policy in Korea is generally treated as means of local developments. This paper aims to examine the localness and socio-economic foundation of social enterprises which are located in the west of Gyeongnam province in Korea and to provide policy recommendations for promoting local resource-based social enterprises. The selection of the case study firms was chosen by considering various factors such as the viability of the firm, location of a company and the types of organization. The research result shows that most of local social enterprises had a viability and profitability to effectively utilize local resources. But it is claimed that the government policy for promoting local social enterprises reveals some limitations to promoting effectively local social enterprises. First, it is necessary to limit the qualification of applying to the government support program. Second, financial support should be changed from hardware-centered programs to software-centered programs such as training and education for human resource development and the business consulting. Finally, it is necessary for the government policy to focus on follow-up programs for firms which are no more capable of receiving the government financial support. For these firms, the government policy needs to focus on facilitating activities of cooperation between local universities and local social enterprise.

• KDI Journal of Economic Policy
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• v.27 no.1
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• pp.211-238
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• 2005

Korea is far behind other OECD countries in economy-wise productivity: Korea's labor productivity in terms of GDP per hour worked is the lowest among OECD countries. Against the existing productivity gap, there is a worrying sign in Korea's investment trend - rapid fall in machinery and equipment investment with slow increase in R&D investment. The challenge facing Korea is how to transform her economy from catching-up model to a knowledge-based one. The paper shows that, in tandem with the structural changes that today's Korean industries are experiencing, industry's innovation system is also changing. Innovation networks are emerging as the result of economy-wise restructuring since the financial crisis of 1997 and, though still not a dominant force, the newly emerging innovation networks will be the main threads of industry's innovation activities in the future. The changes in industrial innovation system would positively contribute in raising the productivity of the Korean economy. The paper contains a case study on Korea's automobile industry in order to highlight some of main characteristics of the structural changes, in addition to a chapter that gives an overview of the evolutionary paths of the Korea's industrial innovation. The paper assesses that changes can be considered as a positive sign of future growth perspective; but there are further challenges to make the Korea's industrial innovation system effective. The list of such challenges includes strengthening upstream sectors of currently leading industries, expanding the innovation base to SME and promoting technological co-operation between domestic firms and foreign firms.

• Journal of Digital Convergence
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• v.13 no.10
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• pp.99-106
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• 2015

The biggest change in modern society because we will transition to a ubiquitous environment. Changes in the environment has become a crucial instrument that finally opens the era of convergence management through integrating the various fields in their own business. The desire of consumers to new innovative products appears to be a constant thing companies are constantly trying to respond to these changes, there may not be a problem for the convergence of sustainability management company in the end. In this study, based on the convergence of corporate management need to be a fusion component of corporate management to examine whether any impact on the quality of innovation. Results showed that the fusion management components that affect both internal factors and external factors, core factors quality improvement. Internal factors detailed in the convergence management leadership, risk management factors showed a positive external factors affecting appeared to affect positively the knowledge-sharing factors, infrastructure factors. Finally, core factor is technology factors, educational learning factors showed a positive impact. This results suggest that be a big impact factors of competitiveness of enterprises through convergence management in the future and will serve as the strategic basis for the convergence of future corporate management.