• Journal of The Korean Association For Science Education
• /
• v.41 no.3
• /
• pp.267-281
• /
• 2021

The shift in the perspective on learning as participation draws attention to learners' identity work. This manuscript foregrounds the ongoing and fluid processes of identity, and focuses on the structure surrounding learners where identity work occurs and the expression of individual agency. From the perspective of learning as participation, we introduce Holland et al. (1998) identity-in-practice theory, as the theoretical lens that dialectically describes structure, agency and identity. The concepts representing this theory are 'figured world', 'positionality' and 'authoring self'. The figured world is a web of meaning encompassing the continuous construct/reconstruct through human actions and also exerts as the social force that shapes human actions. 'Positionality' on the other hand, means an understanding of one's social position in the world. The notion of 'authoring self', is a dimension that synthesizes the above two concepts, which describes the improvisation of one's self through the orchestration and collaboration of various social voices. Next, we analyzed and described various cases of this theory that we found within the scope of science education. However, due to the nature of this theory, which takes a sociocultural approach, international research studies cannot be simply applied to the Korean context. Therefore, we sought to expand the applicability of this theory as a methodological tool, by discussing the directions in which this theory can be used in a domestic context. It is intended that the introduction of this theory, which emphasizes multi-layered, continuous and cumulative identity work, will be used as a resource to further expand the horizon of science education reform that is required for the times.

• Korean Journal of Weed Science
• /
• v.8 no.3
• /
• pp.309-316
• /
• 1988

Several experiments were conducted to investigate the achene viability and growth characteristics of Eclipta prostrata (L.) L. No dormancy and no after-ripening requirement were found for E. prostrata achenes. When achenes were stored at room temperature, germination did not decrease with up to 5 months storage. Large differences in loss of viability of E. prostrata achenes occurred when different dehydration methods were used. Immediate dehydration resulted in high viability, but slow dehydration resulted in severe loss of viability. Achene viability at shallow burial depths (5 and 10 cm deep) was lower under upland soil conditions than under lowland soil conditions. Seedling growth was greatly reduced when flooding to a depth of 10 cm occurred at or before the 4-leaf stage. Flooding after the 4-leaf stage stimulated stem elongation. Branching started from the second week and usually terminated at the tenth week. Leaf size was determined by the branch which are related to the assimilate supply. Flowering of E. prostrata started during the fifth week after emergence, and mature achenes were produced from the sixth week. Ten to 14 days were needed for the achenes to mature. About 14,000 achenes were produced on each plant. Achene production per week increased from the sixth week to the tenth week and thereafter it declined. The average number of achenes per inflorescence decreased with delay in flowering.

• Microbiology and Biotechnology Letters
• /
• v.26 no.6
• /
• pp.537-544
• /
• 1998

The cultivation conditions of transformant Alcaligenes eutrophus AER5 harboring cloned phbC gene for mass production of poly (3-hydroxybutyrate-3-hydroxyvalerate)[P(3HB-3HV)] containing high molar fraction of 3-hydroxyvalerate (3-HV) were investigated. In two-stage batch cultivation, transformant accumulated P(3HB-3HV) containing 52.2 mol% of 3HV compared to 30 mol% of parent strain A. eutrophus H16. The increased 3-HV molar fraction was due to the amplified activity of PHB synthase participating in condensation of 3-HB and 3-HV. To increase efficiency of P(3HB-3HV) accumulation, fructose was added along with precursor compound valerate, and total cell mass and P(3HB-3HV) concentrations remarkably increased, but not 3-HV molar fraction. The effect of magnesium ion showed that P(3HB-3HV) concentration and 3-HV molar fraction were significantly increased upto 6.1 g/L and 71.3 mol% at 0.01 g/L of MgSO$_4$, respectively. The efficiency of several pH adjuster, NaOH, NaOH and (NH$_4$)$_2$SO$_4$, and NH$_4$OH, on total cell mass, p(3HB-3HV) concentration, and 3-HV molar fraction was also compared. To overcome the disadvantage of two-stage cultivation, one-stage intermittent fed-batch cultivation was attempted, such a way 10.0 g/L of fructose was supplied for cell growth at initial 36 hr and then 10.0 g/L of valerate and 5.0 g/L of fructose were applied to induce the accumulation of P(3HB-3HV), consequently, 10.4 g/L of P(3HB-3HV) with 38 mol% of 3-HV fraction could be obtained after 72 hr. These results can be used for elucidating cultivation strategy for mass production of P(3HB-3HV) containing high 3-HV molar fraction using transformant A. eutrophus AER5 harboring cloned phbC gene.

• Applied Biological Chemistry
• /
• v.50 no.2
• /
• pp.95-100
• /
• 2007

The cellulase gene of Bacillus licheniformis K11 which has plant growth-promoting activity by auxin and antagonistic ability by siderophore was cloned in pUC18 using PCR employing heterologous primers. The 1.6kb PCR fragment contained the full sequence of the cellulase gene, denoted celW which has been reported to encode a 499 amino acid protein. Similarity search in protein data base revealed that the cellulase from B. licheniformis K11 was more than 97% identical in amino acid sequence to those of various Bacillus spp. The cellulase protein from B. licheniformis K11, overproduced in E. coli DH5${\alpha}$ by the lac promoter on the vector, had apparent molecular weight of 55 kDa upon CMC-SDS-PAGE analysis. The protein not only had enzymatic activity toward carboxymethyl-cellulose (CMC), but also was able to degrade insoluble cellulose, such as Avicel and filter paper (Whatman$^{\circledR}$ No. 1). In addition, the cellulase could degrade a fungal cell wall of Phytophthora capsici. Consequently B. licheniformis K11 was able to suppress the peperblight causing P. capsici by its cellulase. Biochemical analysis showed that the enzyme had a maximum activity at 60$^{\circ}C$ and pH 6.0. Also, the enzyme activity was activated by Co$^{2+}$ of Mn$^{2+}$ but inhibited by Fe$^{3+}$ or Hg$^{2+}$. Moreover, enzyme activity was not inhibited by SDS or sodium azide.

• Journal of Life Science
• /
• v.29 no.11
• /
• pp.1208-1217
• /
• 2019

Saposhnikovia has been used as a traditional medicinal herb in Asia because of the reported anti-inflammatory, anti-allergic rhinitis, pro-whitening, anti-atopy, anti-allergy, and anti-dermatopathy effects of the phytochemical compounds it contains. In this study, we investigated the antioxidant effects of a Saposhnikovia extract after fermentation by Lactobacillus plantarum BHN-LAB 33. Saposhnikovia powder was inoculated with L. plantarum BHN-LAB 33 and fermented at $37^{\circ}C$ for 72 hr. After fermentation, the total polyphenol content of the Saposhnikovia extract increased by about 14%, and the total flavonoid content increased by about 9%. The superoxide dismutase-like activities, DPPH radical scavenging, ABTS radical scavenging, reducing power activity, and tyrosinase inhibition activity also increased after fermentation by approximately 70%, 80%, 45%, 39%, and 44%, respectively. The results confirmed that fermentation of a Saposhnikovia extract by L. plantarum BHN-LAB 33 is an effective way to increase the antioxidant effects of the extract. The bioconversion process investigated in this study may have the potential to produce phytochemical-enriched natural antioxidant agents with high added value from Saposhnikovia matrices. These results can also be applied to the development of improved foods and cosmetic materials.

• Journal of rehabilitation welfare engineering & assistive technology
• /
• v.11 no.1
• /
• pp.81-89
• /
• 2017

In this paper, we propose a gait pattern recognition method for intelligent prosthesis that enables walking in various environments of femoral amputees. The proposed gait mode changing method is a single sensor based algorithm which can discriminate gait surface and gait phase using only strain gauges sensor, and it is designed to simplify the algorithm based on multiple sensors of existing intelligent prosthesis and to reduce cost of prosthesis system. For the recognition algorithm, we analyzed characteristics of the ground reaction force generated during gait of normal person and defined gait step segmentation and gait detection condition, A gait analyzer was constructed for the gait experiment in the environment similar to the femoral amputee. The validity of the paper was verified through the defined detection conditions and fabricated instruments. The accuracy of the algorithm based on the single sensor was 95%. Based on the proposed single sensor-based algorithm, it is considered that the intelligent prosthesis system can be made inexpensive, and the user can directly grasp the state of the walking surface and shift the walking mode. It is confirmed that it is possible to change the automatic walking mode to switch the walking mode that is suitable for the walking mode.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.65 no.4
• /
• pp.365-376
• /
• 2020

Immature embryogenesis is a useful process in wheat tissue culture, including transgenic technology, because of its high regeneration efficiency compared to that in other tissues. However, it is a very labor-intensive and time-restrictive method, because the preparation of immature embryos is limited to the optimal time after flowering. In this experiment, 'Speed Breeding', a breeding technique that accelerates breeding generation advancement by extending the photoperiod, was applied to the wheat variety 'Bobwhite'. A controlled growth room was constructed by adjusting the photoperiod (22-hour light/2-hour dark) using LED lights at temperature of 22℃. After vernalization of the Bobwhite seeds at 4℃ for 4 weeks, the seedlings were grown in a controlled growth room and a greenhouse to compare the heading date. In both conditions, calli were induced from immature embryos on the 11^th day after flowering. After 4 weeks, the calli were transferred to a regeneration medium. Regeneration efficiencies under greenhouse conditions and Speed Breeding conditions were determined as 45.05% and 43.18%, respectively. Antioxidant enzyme activity and reference gene expression analysis were performed to confirm the presence of stress due to an extremely long-day photoperiod. As a result, the antioxidant enzyme activity was not distinguished from that of the greenhouse condition. The reference gene expression analysis revealed that the PsaA and CDC genes were highly expressed under the Speed Breeding condition. However, expression of PsbA was similar expression in both conditions. These results will provide useful information for the application of immature embryogenesis to the wheat transformation system.

• Applied Chemistry for Engineering
• /
• v.20 no.4
• /
• pp.391-395
• /
• 2009

For the conversion of domestic waste-wood into energy, a fixed bed gasifier ($0.9 m{\times}2.4 m$) having the capacity of 2 ton/day was designed and constructed. The dual knife valve was used to feed waste-wood of which size was 3~5 cm and a rotary stoker system was installed in the bottom of gasifier. The pilot gasification system consisted of feeding system, fixed bed gasifier, gravity fine particle collector, heat exchanger for syngas cooling, ID fan, and cooling tower. The operation temperatures of gasifier were $700{\sim}1000^{\circ}C$ and the concentrations of syngas were CO: 25~40 vol%, $H_2$: 7~12 vol%, $CH_4$: 2~4 vol%, $CO_2$: 12~24 vol%. The calorific value of syngas was $1100{\sim}1500kcal/Nm^3$ and was enough to be applied in the industrial combustor. Also the gas engine was operated by using syngas from biomass gasifier and produced 1~4 kW of power.

• 한국가스학회:학술대회논문집
• /
• 2003.10a
• /
• pp.120-125
• /
• 2003

본 연구는 원자력 발전소에 있는 방화벽의 케이블 관통부위에 설치된 CPFS(Cable Penetration Fire Stop)시스템 안에서 일어나는 동적열전달 현상을 3 차원으로 나타낼 수 있는 시험시뮬레이터에 사용될 수학적 모델과 수치계산 알고리즘의 개발에 관한 것이다. CPFS 내에서 일어나는 열전도 현상을 나타내는 지배방정식은 주어진 조건들 하에서 포물선형 편미분방정식(Parabolic PDE)으로 나타난다. 문제를 단순화하기 위해 열의 흐름을 두 성분으로 나누었다 즉, 케이블과 평행한 선을 따라서 일어나는 열전도와 벽면과 평행한 평면 위에서 일어나는 열전도로 나누었다. 먼저 선을 따라 일어나는 동적 열전도 현상을 나타내는 PDE를 연속과완화(SOR: Successive Over-Relaxation)를 적용하여 유한한 불연속점들에 대한 연립 상미분방정식(ODE)으로 전환했고, 그 연립방정식은 ODE Solver 를 이용하여 풀 수 있었다. 둘째로, 각 불연속 점에 위치한 평면 위에서 일어나는 열전도를 계산하기 위해서, 유한요소의 합을 근사식으로 이용하여 PDE를 ODE로 전환해서 계산하는 유한요소법(Finite Element Method)이 이용된다. 여기서 시간과 공간의 함수 T(x, y, z, t)인 온도는 각 선의 점들과 각 평면의 요소들에 대해서 일정한 시간간격으로 초기온도와 경계온도를 업데이트하여 계산을 반복한다. 이러한 일련의 계산결과를 바탕으로 CPFS 시스템 내에서의 온도분포의 동적인 변화를 해석한다. 결론적으로 관통하는 케이블이 CPFS 시스템의 온도분포에 매우 중요한 역할을 한다는 것을 알 수 있다. 시뮬레이션 결과는 CPFS 내의 온도분포를 쉽게 이해할 수 있도록 3 차원 그래픽으로 나타냈으며, 상용소프트웨어 FEMLAB 으로 계산한 결과와 비교해서 개발된 모델과 계산 알고리즘의 정당성을 보였다. 맞이하고 있음을 볼 수 있다. 국내광업이 21C 급변하는 산업환경에 적응하여 생존하기 위해서는 각종 첨단산업에서 요구하는 소량 다품종의 원료광물을 적기에 공급 할 수 있는 전문화된 기술력을 하루속히 확보해야 하며, 이를 위해 고품위의 원료광물 확보를 위한 탐사 및 개발을 적극 추진하고 가공기술의 선진화를 위해 선진국과의 기술제휴 등 자원산업 글로벌화 정책이 절실히 요구되고 있음을 알 수 있다. 또한 삶의 질을 향상시키려는 현대인의 가치관에 부합하기 위해서는 각종 소비제품의 원료를 제공하는 광업의 본래 목적 이외에도 자연환경 훼손을 최소화하며 개발 할 수밖에 없는 구조적인 어려움에 직면할 수밖에 없다. 이처럼 국내광업이 안고 있는 여러 가지 난제들을 극복하기 위해서는 업계와 정부가 합심하여 국내광업 육성의 중요성을 재인식하고 새로운 마음가짐으로 관련 정책을 수립 일관성 있게 추진해 나가야 할 것으로 보인다.의 연구 결과를 요약하면 다음과 같다. 첫째, 브랜드 이미지와 서비스 품질과의 관계에서 브랜드이미지는 서비스 품질의 선행변수가 될 수 있음을 증명하였으며 4개 요인의 이미지 중 사풍이미지를 제외한 영업 이미지, 제품 이미지, 마케팅 이미지가 서비스 품질에 영향을 미치고 있음을 알 수 있다. 둘째, 지각된 서비스 품질과 가격 수용성과의 관계에서, 서비스 품질은 최소 가격에 신뢰서비스 요인에서 정의 영향을 미치고 있으나 부가서비스, 환경서비스에서는 역의 영향을 미침을 알수 있고, 최대 가격에 있어서는 욕구서비스 요인은 정의 영향을 미치지만 부가서비스의 경우에는 역의 영향을 미치고 있음을 알 수 있다. 셋째, 서비스품질과 재 방문 의도와의 관계에 있어서 서비스품질은 재 방문 의도에 영향을 미침을 알 수 있다. 따라서 브랜드 이미지는 서비스품질의 선행변수가 될 수 있으며, 서비스품질은 가격 수용성과 재방문 의도에 영향을 미치고 있음을 알 수

• Journal of Korean Society of Transportation
• /
• v.32 no.3
• /
• pp.227-238
• /
• 2014

There is no question that railroad investment is crucial for better transport systems and the Korean government has continued to invest in railroad facilities. Nevertheless, the modal share of railroad, in particular, for freight has decreased. This is because rail freight transport can hardly meet customer needs such as just-in-time(JIT), a door-to-door service, compared to road transport, to be specific, trucking which can directly carry the freights to the final destinations. This has made the value of the railroad infrastructure less, which has been operated for the past 114 years. This study proposes a new freight movement concept called Piggy Back System. This system can carry freight trucks on the trains and deliver the freights to the final destinations. It can make a door-to-door deliver system possible for railroad transportation, which is the key factor for modal shift from road to railroad. For implementing this, this study proposes three important things: the cost-efficient reforming way of railroad yard that has been used for the past 114 years, the diagram plan of train services, and some technical reviews like clearance limits. This is the first study with practical proposals and solutions on this topic in Korea. The suggestions of this study cut down distribution costs by more than seven trillion won.