• Journal of Plant Biotechnology
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• v.43 no.3
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• pp.376-382
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• 2016

DNA methylation regulations gene expression, thus having pivotal roles in a myriad of physiological and pathological processes. In this study, the morphology and stress tolerance of transgenic rice overexpressing NtROS2a were determined. Transgenic plants exhibited less and shorter lateral shoots. Under various treatments, rice overexpressing NtROS2a showed alleviation of damage symptoms with higher survival rate. After drought and re-watering treatment, transgenic rice seedlings restored their normal growth. However, wild type plants could not be rescued. These findings indicate that overexpression of NtROS2a gene in rice seedlings can increase their tolerance to drought stresses.

• Korean Journal of Microbiology
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• v.35 no.1
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• pp.53-60
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• 1999

The biodegradation of recalcitrant polycyclic aromatic hydrocarbon, pyrene was investigated in microcosm simulating the beach sand and seawater. The natural biodegradation rates of pyrene were between 30-2,200 ng/g(ml)/day in beach sand and seawater when the pyrenc loading rates were 100- 1,000 ppm at 5-$20^{\circ}C$. The effects of the inoculum size, pyrene concentralion, incubation temperature and surfactant addition were investigated in fertilized (Inipol EAP 22) samples. Generally the biodegradation in beach sand was higher than that in seawater. A mixed inoculum (Pseudomonus, Acinetobacter, Moruxella) showed the 3,120 nglglday of biodegradation rate in beach sand with 200 ppm pyrene, which was 7.8 times higher than the natural biodegradation rate. The highest transformation rate, 4,860 ng/g/day was obtained in the bioaugmented beach sand (1,000 ppm pyrene). The glucose and surfactant addition to enhance the removal have negatively influenced on the biodegradation of pyrene. In case ol surfactants, CMC (critical micell concentration) might bc the control factor for the biodegradation.

• Horticultural Science & Technology
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• v.17 no.6
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• pp.770-772
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• 1999

This study was conducted to provide a basic system to develop a molecular marker for plant cultivar protection using a recombinant DNA technology. Using Nicotiana tabacum L. plants, the potentiality in the utilization of the developed marker was examined. After homology test with several plant genomes, mouse adenosine deaminase (ADA) gene was selected as DNA source of a molecular marker for cultivar protection. As a result of the digestion of ADA gene with BamHI and Pst I, six DNA fragments were obtained, and 513 bp DNA fragment among them was selected as a possible DNA marker for cultivar protection. Selected 513 bp DNA fragment was efficiently inserted into pBI101 plasmid vector for plant transformation by using phagemid vector pBluescript II SK (+/-) as an intermediate vector. The recombinant pBI101, carrying 513 bp DNA fragment, possible markers for cultivar protection, was transformed into A. tumefaciens LBA4404. Nicotiana tabacum was transformed with A. tumefaciens LBA4404 having the recombinant pBI101 and was confirmed the transfer of 513 bp DNA fragment, a possible molecular marker for cultivar protection.

• Communications of Mathematical Education
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• v.34 no.3
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• pp.201-214
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• 2020

In December 2019, new coronavirus (COVID-19) which was started from Wuhan caused a dramatic changes in college mathematics education around the world from the spring semester 2020. All classes in college was switched to online classes at once. As a result, many efforts were made to keep the quality of mathematics education in college. We have discussed the situation that we did experience and discussed a possible changes that future mathematics education and research can face in the post-corona era (after coronavirus, AC era). Finally, we discussed an evaluation method for student activities that can be used in online class evaluation.

• Proceedings of the Korea Water Resources Association Conference
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• 2020.06a
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• pp.306-306
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• 2020

횡월류위어(side weir)는 하천의 수위가 한계수위 이상으로 상승할 경우 본류로부터 저류지나 분수로(distributary channel)로 흐름을 전환하기 위하여 사용하는 수공구조물로 강변저류지나 off-line저류지의 유입부에 흐름방향과 평행하게 설치되어 유량관리 및 전환, 홍수통제, 에너지 소산, 수위조절, 일정 유량의 취수 및 분배, 초과 홍수량의 전환 등의 목적으로 이용되는 구조물이다. 횡월류 위어의 월류 흐름은 일반위어와 같이 위어마루부 직각방향으로 흐르지 않고 본류 흐름특성에 따라 비스듬하게 유입된다. 이러한 흐름특성으로 횡월류위어 월류량은 본류의 하폭, 흐름특성, 위어길이 및 설치위치 등에 따라 각기 다르게 산정된다. 현재 국내에서 진행된 횡월류위어 흐름특성에 관련된 연구는 대부분 직선수로에 집중되어 있으며 사행하천의 흐름특성에 따른 연구는 부족한 실정이다. 금회 연구에서는 3차원 상용프로그램인 FLOW-3D를 이용하여 사행하천구간 유입부 설치위치 특성에 따른 횡월류 위어 유입흐름 특성을 분석하였다. 사행하천 구간 횡월류위어 설치위치에 따른 3차원 흐름해석을 위해 AUTO CAD 프로그램을 이용하여 수로길이 30m, 수로폭 2m의 구형 사행수로를 구성하였고, 횡월류위어 유입부 위치를 20°~120°로 변화시키며 수치모형실험을 수행하였다. 해석결과 수로흐름은 유입부 설치각이 작을수록 상·하류 수위차가 작아지며 유속이 감소하며 설치위치각이 클수록 수로내 평균유속은 증가하는 것으로 확인되었다. 유입부 설치각이 작을수록 방류량이 증가하여 수로내 흐름분리현상 증가하였고 이로인한 지체현상이 발생하는 것으로 확인되었다. 본 연구로 사행하천구간에 횡월류위어가 설치된 경우, 월류량과 수리학적 흐름특성을 해석할 때 3차원 수치모형실험이 유용한 해석도구로 이용될 수 있음이 확인되었다. 이후 수치모형실험이 수공구조물 설계 및 해석 시 참고자료로 이용 가능할 것으로 사료된다.

• Proceedings of the Korea Water Resources Association Conference
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• 2021.06a
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• pp.250-250
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• 2021

횡월류위어(side weir)는 하천의 수위가 한계수위 이상으로 상승할 경우 본류로부터 저류지나 분수로(distributary channel)로 흐름을 전환하기 위하여 사용하는 수공구조물로 강변저류지나 off-line저류지의 유입부에 흐름방향과 평행하게 설치되어 유량관리 및 전환, 홍수통제, 에너지 소산, 수위조절, 일정 유량의 취수 및 분배, 초과 홍수량의 전환 등의 목적으로 이용되는 구조물이다. 횡월류 위어의 월류 흐름은 일반위어와 같이 위어마루부 직각방향으로 흐르지 않고 본류 흐름특성에 따라 비스듬하게 유입된다. 이러한 흐름특성으로 횡월류위어 월류량은 본류의 하폭, 흐름특성, 위어길이 및 설치위치 등에 따라 각기 다르게 산정된다. 현재 국내에서 진행된 횡월류위어 흐름특성에 관련된 연구는 대부분 직선수로에 집중되어 있으며 사행하천의 흐름특성에 따른 연구는 부족한 실정이다. 금회 연구에서는 3차원 상용프로그램인 FLOW-3D를 이용하여 사행하천구간 유입부 설치위치 특성에 따른 횡월류 위어 유입흐름 특성을 분석하였다. 사행하천 구간 횡월류위어 설치위치에 따른 3차원 흐름해석을 위해 AUTO CAD 프로그램을 이용하여 수로길이 30m, 수로폭 2m의 구형 사행수로를 구성하였고, 횡월류위어 유입부 위치를 20°~120°로 변화시키며 수치모형실험을 수행하였다. 해석결과 수로흐름은 유입부 설치각이 작을수록 상·하류 수위차가 작아지며 유속이 감소하며 설치위치각이 클수록 수로내 평균유속은 증가하는 것으로 확인되었다. 유입부 설치각이 작을수록 방류량이 증가하여 수로내 흐름분리현상 증가하였고 이로인한 지체현상이 발생하는 것으로 확인되었다. 본 연구로 사행하천구간에 횡월류위어가 설치된 경우, 월류량과 수리학적 흐름특성을 해석할 때 3차원 수치모형실험이 유용한 해석도구로 이용될 수 있음이 확인되었다. 이후 수치모형실험이 수공구조물 설계 및 해석 시 참고자료로 이용가능할 것으로 사료된다.

• Journal of Plant Biotechnology
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• v.42 no.3
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• pp.186-195
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• 2015

Anthranilate synthase (AS) is a key enzyme in the biosynthesis of tryptophan (Trp), which is the precursor of bioactive metabolites like indole-3-acetic acid and other indole alkaloids. Alpha anthranilate synthase 2 (OsASA2) plays a critical role in the feedback inhibition of tryptophan biosynthesis. In this study, two vectors with single (F124V) and double (S126F/L530D) point mutations of the OsASA2 gene for feedback-insensitive ${\alpha}$ subunit of rice anthranilate synthase were constructed and transformed into wildtype Dongjinbyeo by Agrobacterium-mediated transformation. Transgenic single and double mutant lines were selected as a single copy using TaqMan PCR utilized nos gene probe. To select intergenic lines, the flanking sequence of RB or LB was digested with a BfaI enzyme. Four intergenic lines were selected using a flanking sequence tagged (FST) analysis. Expression in rice (Oryza sativa L.) of the transgenes resulted in the accumulation of tryptophan (Trp), indole-3-acetonitrile (IAN), and indole-3-acetic acid (IAA) in leaves and tryptophan content as a free amino acid in seeds also increased up to 30 times relative to the wildtype. Two homozygous event lines, S-TG1 and D-TG1, were selected for characterization of agronomic traits and metabolite profiling of seeds. Differentially expressed genes (DEGs), related to ion transfer and nutrient supply, were upregulated and DEGs related to co-enzymes that work as functional genes were down regulated. These results suggest that two homozygous event lines may prove effective for the breeding of crops with an increased level of free tryptophan content.

• Journal of Plant Biotechnology
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• v.34 no.4
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• pp.277-283
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• 2007

Embryogenic calluses derived from shoot apical meristem explants of sweetpotato were subjected to particle bombardment to generate transgenic plants for antisense expression of cDNAs encoding two different AGPase small subunit (ibAGP1 and ibAGP2). Plants were generated via somatic embryogenesis. PCR and Southern analysis demonstrated that the incorporation of ibAGP1 and ibAGP2 into the genome in an antisense orientation. Immunoblot analysis confirmed reduced levels of AGPase small subunit in transgenic plant leaves. Plants with both ibAGP1 and ibAGP2 produced a lower level of the protein than plants with ibAGP1 alone. iodine test demonstrated that transgenic plant leaves and storage root accumulated reduced amounts of starch. Iodine staining of leaf tissues indicated that transgenic plants accumulated less amount of starch than control. In accordance with western blot analysis, plants with both ibAGP1 and ibAGP2 accumulated a lower amount of starch than plants with ibAGP1 alone. Both transgenic plants exhibited a severely retarded growth, resulting in bare survival. It is suggested that disrupted expression of the gene encoding AGPase small subunit is lethal to the growth of sweetpotato contrast to other species including potato.

• Journal of Life Science
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• v.27 no.2
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• pp.194-201
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• 2017

Rapeseed (Brassica napus L.) is an oil crop classified as Brassicaceae, and it is widely grown worldwide. To develop a drought-resistant rapeseed, the ${\beta}$-glucosidase 1 (AtBG1) gene was introduced into rapeseed because drought- and salt-resistance phenotypes were observed when the AtBG1 gene was overexpressed in arabidopsis. Newly developed genetically modified crop must be proved to be safe. Safety assessments are based on the historical usage and scientific reports of a crop. In this study, we examined the potential acute oral toxicity of AtBG1 protein expressed in genetically modified (GM) rapeseed and calculated the minimum lethal dose at 6 weeks in both male and female ICR mice. AtBG1 protein was fed at a dose of 2,000 mg/kg body weight in five male and five female mice according to the marginal capacity concentration of OECD, 2,000 mg/15 ml/kg. Mortalities, clinical findings, and body weight changes were monitored for 14 days after dosing, and postmortem necropsy was performed on day 14. This study showed that no deaths occurred in the test group, and AtBG1 protein did not result in variations in common symptoms, body weight, and postmortem findings between the two groups. This showed that the minimum lethal dose of AtBG1 protein expressed in transgenic rapeseed exceed 2,000 mg/kg body weight in both sexes.

• Journal of Korean Society of Transportation
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• v.20 no.5
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• pp.33-43
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• 2002

Travel choice behavior is affected by real-time traffic information. Recently, in urban area, real-time traffic information is provided by several instruments such as transportation broadcasting, internet PC network and variable message sign, etc. Furthermore, it has been increasing for urban travelers to use real-time traffic information provided by several instruments. The purpose of this study is to analyze the effects of advanced traveler information on urban worker's travel choice behavior. Among several Advanced Traveler Information System(ATIS) employed in urban area. This study focuses on examining the effects of transportation broadcasting on urban worker's travel choice behavior. This study attempts to examine traveler's mode change behavior in the pre-trip stage and traveler's route change behavior in the on-route stage. For this study, the survey data collected from Daegu City in 2000 is used. For empirical analysis, several nested logit models are estimated, and among them, the best models are reported in this paper. Furthermore, based on the empirical models estimated for this research, important findings and their policy implications are discussed.