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Transfer of Bacillus thuringiensis toxin gene into Bacillus subtilis and its inoculation effects (식물 생장촉진 미생물의 외부 유전자 도입과 그 접종효과)

  • Rhee, Young-Hwan;Kim, Kwang-Sik;Kim, Yong-Woong;Kim, Yeong-Yil
    • Applied Biological Chemistry
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    • v.35 no.5
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    • pp.361-366
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    • 1992
  • The antagonistic bacteria, showing distinguished effect against Fusarium oxysporum and Rhizoctonia solani were isolated from the rhizosphares of horticultural plants and identified as Bacillus subtilis. The strains were studied for their chracteristics of biochemistry, physiology, antagonistic effect against plant pathogenic fungi, and growth promoting effect on horticultural plants. The Bacillus thuringiensis(BT) HD-1 toxin gene was introduced into these B. subtilis. The BT toxin genes on chromosome of the bacteria were identified by southern blotting, but its proteins were not detected by SDS-PAGE. These transformed bacteria showed growth promoting effect and showed also insecticidal and antagonistic effects against Bombix mori and fungi F. oxysporum and R. solani but not against nematode Bursaphelenchus xylophilus.

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Evaluation of Service Life in RC Column under Chloride Attack through Field Investigation: Deterministic and Probabilistic Approaches (염해 실태조사를 통한 철근 콘크리트 교각의 내구수명 평가 - 결정론적 및 확률론적 해석방법)

  • Kwon, Seung-Jun
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.19 no.5
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    • pp.67-74
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    • 2015
  • RC (Reinforced Concrete) structures are considered as cost-benefit and durable however performances of structural safety and durability are degraded due to steel corrosion. Service life in RC structure is differently evaluated due to different local environmental conditions even if it is exposed to the same chloride attack. In the paper, 25 concrete cores from field investigation are obtained from 4 RC columns with duration of 3.5~4.5 years exposed to sea water. Through total chloride content measurement, surface chloride contents and apparent diffusion coefficients are evaluated. Service life of the target structure is estimated through deterministic method based on Fick's $2^{nd}$ Law and probabilistic method based on durability failure probability, respectively. Probability method is evaluated to be more conservative and relatively decreased service life is evaluated in tidal zone and splash zone over 40.0 m. Chloride penetration behavior with coring location from sea level and the present limitations of durability design method are investigated in the paper.

Sporulation and Dissemination of Pycnidiospores of Diaporthe citri in Yuzu Tree (Citrus junos Sieb) in Jeonnam Area (전남지역 유자과원의 검은점무늬병균 포자 형성과 비산)

  • Hur, Kil-Hyun;Park, Seur-Kee
    • Research in Plant Disease
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    • v.11 no.1
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    • pp.16-20
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    • 2005
  • Several time-course experiments were carried out to understand seasonal development of melanose on yuzu trees at koehung area, Jeonnam province, during May to October. The occurrence of dead twigs, known as a source of infection, was much more in older trees, and from June to August, mostly in July. In the experiment of pycnidia development on dead twigs seasonally collected, the number of developed pycnidia was highest on July-collected dead twigs especially with the diameter of 1.1~1.5 cm. In the collection survey of disseminated pycnidiospores, although the collected number of pycnidiospores was affected with amount of precipitation, the number of observed pycnidiospores in rainwater was relatively high from June to August, with highest in early August in 1997 and late July in 1998. In the inoculation tests on 3-year-old trees and fruits in natural condition, disease occurrences were mostly affected on twigs by inocula treatment in June, and on fruits by inocula treatment in July, respectively.

Effects of Various Plant Growth Promoting Rhizobacteria on the Early Growth of Red Pepper Seedlings, Capsicum annuum L. cv. Nockkwang (數種의 植物生長促進 根圈細菌이 '녹광' 고추 幼苗의 初期生長에 미치는 影響)

  • Cho, Ja-Yong;Chung, Soon-Ju
    • Korean Journal of Organic Agriculture
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    • v.7 no.1
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    • pp.137-146
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    • 1998
  • Various rhizobacteria was isolated, and was bacterialized into the substrates to clarify the plant growth promoting effects of rhizobacteria on the early growth of red peper seedlings. Total 125 bacterial isolates were primarily isolated and purified from the soils in greenhouse. And four strains were finally screened, based on the antifungal activities against Fusarium sp., Pythium sp. and Rhizoctonia sp. of red pepper plants. The strongest antifungal strain RB 109 has a antagonistic activity against Fusarium sp., Pythium sp. and Rhizoctonia sp. in terms of 66.0%, 65.0% and 66.1%. Early growth of red pepper seedlings was promoted, when cultured solution of rhizobacteria RB 109 was bacterialized into the substrates. Antifungal rhizobacteria RB 109 was identified as Pseudomonas sp. related strains, which has a similarity of 82% to the Pseudomonas sp.

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Effects of Grafting Cultivation on the Growth and Yield of Paprika in Highlands (고랭지 착색단고추의 접목재배 효과)

  • Lee, Jong-Nam;Lee, Eung-Ho;Kim, Jeom-Sun;Kim, Won-Bae;Ryu, Seung-Yeol;Yong, Yeong-Rok
    • Journal of Bio-Environment Control
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    • v.13 no.1
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    • pp.33-38
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    • 2004
  • This experiment was conducted to screen the suitable rootstocks for the soil cultivation of paprika (Capsicum annuum L.) in highland. Sixteen kinds of rootstocks were grafted to the red colored 'Spirit' scion for the resistances to diseases like fruit lot (Phytophthora capsici) and bacterial wilt (Ralstonia solanacearum). Four varieties among the rootstocks, 'Tantandaemok', 'Skurt-S', 'AC 2258', and 'PST 8301' were selected for the high resistance to fruit rot of paprika. However non-grafted control plants were totally dead at five days after inoculation. Furthermore, seven varieties including 'Yeokgang', ;Tantandaemok', 'TE412', 'MC 4', 'PST VK', and 'PST NV' were selected for the high resistance to bacterial wilt. The grafting with pest resistant rootstocks could enhance the rhizophere environment through root fresh weight increase. High yielding rootstocks for paprica 'Spirit' were PST 8301, MC 4, and Wanggeun.

NFATc Mediates Lipopolysaccharide and Nicotine-Induced Expression of iNOS and COX-2 in Human Periodontal Ligament Cells (사람 치주인대세포에서 Lipopolysaccharide와 니코틴으로 유도된 iNOS와 COX-2 발현에 NFATc의 관여)

  • Lee, Sang-Im;Yu, Ji-Su
    • Journal of dental hygiene science
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    • v.15 no.6
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    • pp.753-760
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    • 2015
  • Although nuclear factor of activated T cell (NFAT) plays a key role in inflammation, its anti-inflammatory effects and mechanism of action in periodontitis are still unknown. This study aimed to identify the effects of NFAT on the proinflammatory mediators activated by lipopolysaccharide (LPS) plus nicotine stimulation in human periodontal ligament cells (hPDLCs). The production of nitric oxide (NO) and prostaglandin $E_2(PGE_2)$ was evaluated using Griess reagent and an enzyme immunoassay, respectively. The expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and NFAT proteins was evaluated by Western blot analysis. LPS plus nicotine synergistically induced the production of NO and $PGE_2$ and increased the protein expression of iNOS, COX-2 and NFAT. Treatment with an NFAT inhibitor blocked the LPS plus nicotine-stimulated NO and $PGE_2$ release as well as the expression of iNOS and COX-2. Our data suggest that the LPS plus nicotine-induced inflammatory effects on hPDLCs may act through a novel mechanism involving the action of NFAT. Thus, NFAT may provide a potential therapeutic target for the treatment of periodontal disease associated with smoking and dental plaque.

Information Spillover Effects among the Stock Markets of China, Taiwan and Hongkon (국제주식시장의 정보전이효과에 관한 연구 : 중국, 대만, 홍콩을 중심으로)

  • Yoon, Seong-Min;Su, Qian;Kang, Sang Hoon
    • International Area Studies Review
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    • v.14 no.3
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    • pp.62-84
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    • 2010
  • Accurate forecasting of volatility is of considerable interest in financial volatility research, particularly in regard to portfolio allocation, option pricing and risk management because volatility is equal to market risk. So, we attempted to delineate a model with good ability to forecast and identified stylized features of volatility, with a focus on volatility persistence or long memory in the Australian futures market. In this context, we assessed the long-memory property in the volatility of index futures contracts using three conditional volatility models, namely the GARCH, IGARCH and FIGARCH models. We found that the FIGARCH model better captures the long-memory property than do the GARCH and IGARCH models. Additionally, we found that the FIGARCH model provides superior performance in one-day-ahead volatility forecasts. As discussed in this paper, the FIGARCH model should prove a useful technique in forecasting the long-memory volatility in the Australian index futures market.

Infestation Status of the Darkling Beetle (Alphitobius diaperinus) in Broiler Chicken Houses of Korea (육계농장에서 외미거저리(Alphitobius diaperinus) 발생양상 보고)

  • Nguyen, Namhai;Yang, Byung-Kun;Lee, Jae-Seon;Yoon, Jong-Ung;Hong, Ki-Jeong
    • Korean journal of applied entomology
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    • v.58 no.3
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    • pp.189-196
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    • 2019
  • While the darkling beetle, Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae) is considered a pest of stored products, it does not pose a significant economic threat to this industry. The grains these beetles feeding on are often already damaged. However, the beetle is now being treated as the foremost premise pest in the poultry industry in many countries of the world. Recently, the darkling beetle has also infested in broiler chicken houses of Korea. The problems caused by this pest destroy the insulate facilities of poultry houses, reduce chick's overall performance, transmit pathogenic organisms, and have been leading to substantial economic losses for poultry producers. Therefore, we conducted surveys on the status of infestation and damage of this pest in broiler chicken houses. Because there is no simple one step process that will completely eliminate darkling beetle populations from broiler facilities, we would like to outline further studies on the proper control measures.

A Study on the Simulation and Analysis of the Emergency Response Training for Highly Pathogenic Avian Influenza (조류인플루엔자 재난대응훈련 시뮬레이션 기술연구)

  • Kang, Min-Sik
    • The Journal of the Korea institute of electronic communication sciences
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    • v.14 no.1
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    • pp.19-26
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    • 2019
  • Simulation using the virtual reality has been applied in various fields such as exercise, education, disaster simulation training, but there is a little research on disease caused by virus transmission. In this study, we conducted simulation studies and analysis of avian influenza disaster response training. The annual avian influenza virus is recurring every year in Korea, but there are still few solutions and preventive measures for the preventing the avian influenza. The avian influenza can cause a great deal of societal harm and enormous economic damage. Prophylaxis is important because livestock epidemics, such as avian influenza and foot-and-mouth disease, have a large impact on farm households. Therefore, we proposed and analyzed contents that can be avoided through simulation of avian influenza disaster response presented in this study.

Selection of Antifungal Bacteria Burkholderia lata CAB13001 for Control on Red Pepper Anthracnose and Its Control Efficacy in Field (고추 탄저병 방제제 Burkholderia lata CAB13001 선발 및 포장방제 효과)

  • Hahm, Soo-Sang;Kim, Byung-Ryun;Kwon, Mi-Kyung;Han, Kwang-Seop;Park, In-Hee;Seo, Kyung-Won
    • Korean Journal of Organic Agriculture
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    • v.26 no.4
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    • pp.649-660
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    • 2018
  • To control the pepper anthracnose caused by Colletotrichum acutatum, antifungal bacterium strains which was selected among bacterium from natural soil, was tested the antimicrobial activity against various pathogens and its control efficacy on anthracnose disease in the fields. We confirmed that antagonistic activity of CAB13001 strain to pathogens such as Sclerotinia cepivorum, Sclerotinia sclerotium and Botrytis cinerea including Colletotrichum acutatum was remarkable superior with the dual culture method in the artificial medium. In vitro bioassay using the green pepper fruit, CAB13001 strain suppressed the lesion development of Anthracnose disease, and its control value compared to the untreated one was 82.4% on pepper fruit in field test. These results suggested that CAB13001 strain could be a very useful biological control agents to anthracnose disease caused by air born plant pathogens of pepper. By the way, analysis of nucleotide sequence of the gene 16S rDNA, antagonistic bacterium CAB13001 strain used in this study was identified as Burkholderia lata.