Korean Journal of Organic Agriculture (한국유기농업학회지)

Korean Association of Organic Agriculture (한국유기농업학회)
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Selection of Antifungal Bacteria Burkholderia lata CAB13001 for Control on Red Pepper Anthracnose and Its Control Efficacy in Field

고추 탄저병 방제제 Burkholderia lata CAB13001 선발 및 포장방제 효과

  • 함수상 (충청남도농업기술원 친환경농업과) ;
  • 김병련 (충청남도농업기술원 친환경농업과) ;
  • 권미경 (충청남도농업기술원 친환경농업과) ;
  • 한광섭 (충청남도농업기술원 친환경농업과) ;
  • 박인희 (충청남도농업기술원 친환경농업과) ;
  • 서경원 (전라북도농업기술원 농식품개발과)
  • Received : 2018.08.14
  • Accepted : 2018.10.22
  • Published : 2018.11.30
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Abstract

To control the pepper anthracnose caused by Colletotrichum acutatum, antifungal bacterium strains which was selected among bacterium from natural soil, was tested the antimicrobial activity against various pathogens and its control efficacy on anthracnose disease in the fields. We confirmed that antagonistic activity of CAB13001 strain to pathogens such as Sclerotinia cepivorum, Sclerotinia sclerotium and Botrytis cinerea including Colletotrichum acutatum was remarkable superior with the dual culture method in the artificial medium. In vitro bioassay using the green pepper fruit, CAB13001 strain suppressed the lesion development of Anthracnose disease, and its control value compared to the untreated one was 82.4% on pepper fruit in field test. These results suggested that CAB13001 strain could be a very useful biological control agents to anthracnose disease caused by air born plant pathogens of pepper. By the way, analysis of nucleotide sequence of the gene 16S rDNA, antagonistic bacterium CAB13001 strain used in this study was identified as Burkholderia lata.

Colletotrichum acutatum에 의해 발생하는 고추탄저병을 방제하기 위해 토양 근권으로부터 분리한 미생물 중 길항효과가 있는 미생물을 선발하여 주요 작물병원균에 대한 항균활성 효과와 포장에서의 방제효과를 검정하였다. 기내 대치배양법을 이용한 길항력을 검정하였을 때, CAB13001 균주는 고추 탄저병을 일으키는 Colletotrichum acutatum을 포함한 식물 병원균 Sclerotinia cepivorum, Sclerotinia sclerotium, Botrytis cinerea에 대한 뛰어난 항균활성 효과를 보였다. 또한 풋고추를 이용한 생체 검정에서 선발균주는 고추 탄저병의 병반진전 억제효과가 우수하였으며, 포장에서의 방제가 역시 무처리 대비 82.4%로 매우 우수하였다. 이러한 결과로 볼 때 CAB13001 균주는 공기 전염을 하는 고추 탄저병의 생물적 방제제로 매우 유용할 것으로 판단된다. 한편, 본 연구에 사용된 길항미생물 CAB13001 균주는 16s rRNA gene의 계통학적 위치를 분석한 결과 Burkholderia lata로 동정하였다.

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References

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