• The Korean Journal of Mycology
• /
• v.47 no.1
• /
• pp.1-12
• /
• 2019

This study was conducted to investigate the optimum culture conditions, for mass production of Tremella fuciformis in M9 basic medium. The strain KMCC04674, used in this study was identified T. fuciformis by internal transcribed spacer (ITS). To define the optimum conditions for the mass production of T. fuciformis, we investigated the effects of different culture conditions and various nutrient sources on the fungal growth. The optimum initial pH and temperature for the fungal growth were 5.0 and $25^{\circ}C$, respectively. The optimal composition of the growth medium was 4.0% mannitol, 3.0% $NH_4H_2PO_4$, 1.0% malt extract, 1.0% glutamic acid, 5mM $CaCl_2$, and 0.5% glucanic acid.

• Journal of Environmental Health Sciences
• /
• v.45 no.1
• /
• pp.82-96
• /
• 2019

Objective: The aims of this study were to provide the basic data for establishing a precautionary management policy and to develop a methodology for selecting a radon management priority target area suitable for the Korean domestic environment. Methods: A suitable mapping method for the domestic environment was derived by conducting a quantitative comparison of predicted values and measured values that were calculated through implementation of two models such as IDW and RBF methods. And a qualitative comparison including the clarity of information transmission of the written radon map was carried out. Results: The predicted and measured values were obtained through the implementation of the spatial analysis models. The IDW method showed the lowest in the calculated mean square error and had a higher correlation coefficient than the other methods. As results of comparing the uncertainty using the jackknife concept and the concept of error distance for comparison of the differences according to the model interpolation method, the sum of the error distances showed a modest increase compared with the RBF method. As a result of qualitatively comparing the information transfer clarity between the radon maps prepared with the predicted values through the model implementation, it was found that the maps plotted using the predicted values by the implementation of the IDW method had greater clarity in terms of highness and lowness of radon concentration per area compared with the maps plotted by other methods. Conclusions: The radon management priority area suggests selecting a metropolitan city including an area with a high radon concentration.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.20 no.7
• /
• pp.145-150
• /
• 2019

The purpose of this study was to investigate farmers' field survey to improve animal welfare certification standards and to obtain basic data on the animal welfare level for certified farmers to develop evaluation methods of animal welfare level suitable for domestic farm environment. The subjects of the study were selected 10 animal welfare laying certified farmhouses. The farming certified farming facility survey collected breeding form, head, feed and drink space, breeding density, length and shape of perch. Animal welfare was assessed by the presence of sand bath, spawning, enrichment measures, free range, feathers pecking. The results of the study showed that most the certified animal welfare laying hens complied with the certification standards. All the farms were providing the nest box, but there were farms with more than 20% of the spawning rate. The perches were provided in sufficient length, but only three of ten farms were using rounded perches. Feather damage has been identified in most survey farms, which is likely to be due to feather damage caused by roosters producing both fertilized eggs. In this study, 10 farm households were surveyed. It was not possible to represent the whole certified farmhouse. Therefore, it should be analyzed thoroughly to evaluate the level of animal welfare.

• Korean Journal of Clinical Laboratory Science
• /
• v.51 no.2
• /
• pp.125-133
• /
• 2019

A microscope is the fundamental research and diagnostic apparatus for clinical investigation of signaling transduction, morphological changes and physiological tracking of cells and intact tissues from patients in the biomedical laboratory science. Proper use, care and maintenance of microscope with comprehensive understanding in mechanism are fully requested for reliable image data and accurate interpretation for diagnosis in the clinical laboratory. The standard operating procedure (SOP) for light microscopes includes performance procedure, brief information of all mechanical parts of microscopes with systematic troubleshooting mechanism depending on the laboratory capacity. Maintenance program encompasses cleaning objective, ocular lenses and inner optics; replacement and calibration of light source; XY sample stage management; point spread function (PSF) measurement for confocal laser scanning microscope (CLSM); quality control (QC) program in fluorescent microscopy; and systematic troubleshooting. Laser safety is one of the concern for medical technologists engaged in CLSM laboratory. Laser safety guideline based on the laser classification and risk level, and advisory lab wear for CLSM users are also expatiated in this overview. Since acquired image data presents a wide range of information at the moment of acquisition, well-maintained microscopes with proper microscopic maintenance program are impulsive for its interpretation and diagnosis in the clinical laboratory.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.46 no.1
• /
• pp.85-92
• /
• 2019

The purpose of this study was to compare and evaluate the cytotoxicity of 3 calcium silicate-based materials (CSMs) on stem cells from human exfoliated deciduous teeth (SHEDs). The powder of Retro $MTA^{(R)}$ (RM), $EZ-Seal^{TM}$ (EZ) and ENDOCEM $Zr^{(R)}$ (EN) was eluted with SHED culture media and then filtered. The SHEDs were cultured in the presence of the various concentrations of the eluate. To investigate the effect of the 3 CSMs on SHED proliferation, the MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay was performed. Flow cytometry analysis was also performed to identify any changes in the cellular phenotype. The absorbance values of the SHEDs cultured in the eluate of samples at a 10% concentration showed the following relation: RM > EN > EZ (p = 0.0439). However, the SHEDs maintained their mesenchymal phenotype regardless of product exposure. Although the 3 CSMs did not alter the SHED stem cell markers, EZ may be a less cytocompatible than RM and EN.

• Korean Journal of Construction Engineering and Management
• /
• v.20 no.3
• /
• pp.22-30
• /
• 2019

The purpose of this study is to derive basic data of the Guaranteed Maximum Price(GMP) process and to present specimens that can be used at the time of execution of CM at Risk project in South Korea. CM at Risk project is issued in South Korea, but the actual data is lack. On the other hand, CM at Risk project is universalized and the market of the CM at Risk is steadily growing. Because of that, in this study, The United States cases was selected as a sample. GMP consists of CM's fee, CM contingency, project direct cost, project indirect cost, allowance. GMP negotiations are performed before the end of design completion, and after 3 rounds of estimation comparison, preliminary GMP is confirmed. GMP can be multiple contract and it is useful for Procurement of long lead materials and early construction. If the actual cost is lower than expected GMP, sharing of the saving is an option since the conflict of interests between the client and the contractor can occur.

• Journal of Industrial Convergence
• /
• v.17 no.1
• /
• pp.7-16
• /
• 2019

Korea, which became an aging society in 2000, has made transition to an aged society more quickly than expected since late 2017 after 17 years. Despite this trend, the awareness towards elderly housing facilities has not yet been raised across in society. The primary reason is that use of elderly housing facilities has not been promoted due to lack of understanding about the construction and management of the facilities. The housing of the elderly should be considered in terms of not only residential space but also the issues of medical care and continuous care. Therefore, the trend of elderly housing is pursuing the Continuing Care Retirement Community. Thus, this study analyzed the policies of existing elderly housing facilities and existing elderly housing through comparison with policies and housing of foreign countries. Based on the results, the the government intends to present characteristics and direction of suitable housing design of senior citizens' housing facilities through maintenance of UNIT, securing convergence through changes in the use of facilities, and realising the cost of operation, and studying social welfare measures to cope with the increasing number of elderly residents.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
• /
• v.17 no.1
• /
• pp.29-36
• /
• 2019

High-level radioactive waste (HLW) such as spent fuel is inevitably produced when nuclear power plants are operated. A geological repository has been considered as one of the most adequate options for the disposal of HLW, and it will be constructed in host rock at a depth of 500~1,000 meters below ground level with the concept of an engineered barrier system (EBS) and a natural barrier system. The compacted bentonite buffer is one of the most important components of the EBS. As the compacted bentonite buffer is located between disposal canisters with spent fuel and the host rock, it can restrain the release of radionuclides and protect canisters from the inflow of groundwater. Because of inflow of groundwater into the compacted bentonite buffer, it is essential to investigate soil-water characteristic curves (SWCC) of the compacted bentonite buffer in order to evaluate the entire safety performance of the EBS. Therefore, this paper conducted laboratory experiments to analyze the SWCC for a Korean Ca-type compacted bentonite buffer considering dry density, confined or unconfined condition, and drying or wetting path. There was no significant difference of SWCC considering dry density under unconfined condition. Furthermore, it was found that there was higher water suction in unconfined condition that in confined condition, and higher water suction during drying path than during wetting path.

• Journal of Korea Society of Industrial Information Systems
• /
• v.24 no.2
• /
• pp.33-40
• /
• 2019

Many companies have tried to develop wind power generators with a larger capacity, smaller size and lighter weight. High temperature superconducting (HTS) generators are more suitable for wind power systems because they can reduce volume and weight compared with conventional generators. However, the HTS generator has problems such as huge vacuum vessel and the difficulty of repairing the HTS field coils. These problems can be overcome through the modularization of the HTS field coil. The HTS module coil require a current leads (CLs) for deliver DC current, which causes a large heat transfer load. Therefore, CLs should be designed optimally for reducing the conduction and Joule heat loads. This paper deals with a structural design and thermal analysis of a module coil for a 750 kW-class HTS generator. The conduction and radiation heat loads of the module coils were analysed using a 3D finite element method program. As a result, the total thermal load was less than the cooling capacity of the cryo-cooler. The design results can be effectively utilized to develop a superconducting generator for wind power generation systems.

• The Journal of the Convergence on Culture Technology
• /
• v.5 no.2
• /
• pp.247-255
• /
• 2019

This study was conducted to explore the correlation between the personality types and learning styles and academic achievement in first year nursing students. 144 students were sampled in first year nursing sstudents. Data were collected from March 6 to May 18 2018. Results analysis was performed using SPSS win version of frequency, mean and standard deviation, t-test, ANOVA, Chi-square test, Pearson correlation. The personality types of nursing students in this study were 58.3% of Extrovert type, 41.7% of Introvert type, 33.3% of Sensing, 66.7% of Intuition type, 22.2% of Thinking, 77.8% of Feeling, 50.7% of Judging type, and 49.3% of Perceiving type. The average academic achievement of female students was 3.51 points, higher than the average of 3.17 for male students (t=-3.277, p<.001). The average academic achievement of Introvert type was found to be higher than the average of Extrovert type (t=3.541, p<.001). Learning styles by personality type showed a statistically significant difference between the judging type and the other personality types (${\chi}^2=18.409$, p<.001). There was a significant amount of correlation between gender and TF index (r=.209, p<.05), gender and academic achievement (r=.265, p<.01), JP index and learning styles (r=.262, p<.01, EI index and academic achievement (r=.285, p<.01). The development and utilization of teaching-learning methods suitable for individuals will be required based on the results of personality types, learning styles and academic achievement in first year nursing students identified through this study.