• Korean Journal of Breeding Science
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• v.43 no.1
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• pp.56-61
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• 2011

Brown planthopper(BPH) is one of the most destructive insect pests of rice. The use of genetically resistant cultivars has proven to be a more economical, efficient and environment friendly means to combat this pest. This study was carried out to investigate the relationship between BPH resistant gene, Bph18 and yield components of rice using DH(doubled haploid) lines derived from 'Saenuri'/SR30071-3-7-23-6-1-1-1. SR30071-3-7-23-6-1-1-1 line has Bph18 gene derived from wild species, Oryza australiensis. BPH resistant gene, Bph18 shortened heading days, enlarged culm length and panicle length and reduced ratio of ripened grains of rice. The results indicate that backcrossing breeding is necessary to develop elite cultivars carrying Bph18 gene.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2022.09a
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• pp.53-53
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• 2022

대한민국의 농업생명자원 중 식물유전자원은 농촌진흥청 국립농업과학원 농업유전자원센터와 지방자치단체, 대학, 연구소 등 전국 79개 관리기관에서 보존하고 있으며 책임기관인 농업유전자원센터에서는 종자 1,599종 246,097자원, 관리기관은 영양체자원 1,476종 26,254자원을 관리하고 있다. 유전자원 관리(수집/증식/등록/평가/분양 등) 업무 중 생산되는 대량의 정보를 관리하기 위해서 농업유전자원 정보를 DB 구축하였다. 구축된 정보는 학명 등 기초정보, 형태·저항성 등 특성 정보, 화상·종자량·활력·분양·증식 등으로 구분하여 데이터베이스화하고, 이 정보를 관리하기 위한 농업유전자원관리시스템(Germplasm Management System, GMS)을 운영하였으나 개선전 프로그램은 설치 후 주기적인 업데이트를 해야하고, 하드웨어 교체시 프로그램을 재설치를 해야하는 번거로움이 존재하였다. 이러한 문제를 해결하기 위해 HTML5 방식으로 개선하였다. 개선 프로그램은 웹 브라우저(크롬, 마이크로소트 엣지 등)에 실행 주소(genebank.rda.go.kr/gms)를 입력만 하면 전국 79개 관리기관에서 자원을 접수하고 평가한 정보를 어디에서든 확인이 가능하다(메뉴사용 및 권한설정은 필요). 개선된 농업유전자원 정보관리시스템은 국가적 차원의 통합관리를 지원하여 농업생명자원을 효율적으로 관리하고 수요자가 원하는 자원에 대한 적절한 정보제공을 함으로써 농업생명자원의 활용 촉진에 기여할 것으로 기대된다.

• Journal of Sericultural and Entomological Science
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• v.24 no.1
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• pp.28-31
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• 1982

Flacherie virus (FV) is an important pathogen in the silkworm, which often gives serious damage to farmers for cocoon production. The inbred parents and F$_1$'s from an eight-parent diallel were examined to determine the inheritance of resistance to flacherie virus in the silkworm. Three resistant (R), two intermediate (M) and three susceptible (S) inbreds were used in the diallel with no reciprocals. Mean resistance was measured by survival rates of larvae which were fed on mulberry leaves sprayed with diluted mid-gut homogenate of FV infected larvae. Broad-sense heritability was obtained according to inbreds and F$_1$ family performance. Estimation of general (GCA) and specific combining ability (SCA) was made according to Griffing's Model 1, Method 2. Mean FV resistance of F$_1$ family displayed additive effect of the major gene, while heterotic effect was not significant. Considerable variation in FV resistance within F$_1$ groups of R$\times$S and S$\times$S indicated that action of minor genes for FV resistance may have been involved. FV resistance of inbreds perse and predominant effect of the major gene over minor gene(s) satisfactorily predicted the FV resistance of the hybrids. Broadsense heritability value of FV resistance on the basis of F$_1$ family performance averaged 93%, which suggested that environmental effects might have not been important in this experiment. GCA was highly significant for FV resistant among inbreds. GCA effect of 13.1 in Jam 108 was highest and -17.7 in Gyeongchu lowest. Effective selection for high FV resistance would be possible, using inbreds with high GCA effect and low GCA variance. SCA was significant among hybrids. High SCA effect in the hybrid of Geumho$\times$Mudeung (13.7) and Hansaeng #4$\times$Jam 115 (11.6) indicated that the interaction effect of minor genes for resistance to FV in the silkworm could be exploited by standard silkworm breeding procedures.

• Proceedings of the Korean Society of Tobacco Science Conference
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• 1997.10a
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• pp.134-152
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• 1997

Plant viruses of tobacco including tobacco mosaic virus (TMV) and potato virus Y (PVY) cause severe economic losses in leaf-tobacco production. Cultural practices do not provide sufficient control against the viruses. Use of valuable resistant cultivars is most recommendable for the control of the viruses. However, conventional breeding programs are not always proper for the development of virus-resistant plants mostly owing to the frequent lack of genetic sources and introduction of their unwanted properties. Therefore, we tried to develop virus-resistant tobacco plants by transforming commercial tobacco cultivars, NC 82 and Burley 21, with coat protein (CP) or replicase (Nlb) genes of TMV and PVY necrosis strain (PVY-VN) with or without untranslated region (UTR) and with or without mutation. Each cDNA was cloned and inserted in plant expression vectors with 1 or 2 CaMV 35S promotors, and introduced into tobacco leaf tissues by Agrobacterium tumefaciens LBA 4404. Plants were regenerated in kanamycin-containing MS media. Regenerated plants were tested for resistance to TMV and PVY In these studies, we could obtain a TMV-resistant transgenic line transformed with TMV CP and 6 genetic lines with PVY-VN cDNAs out of 8 CP and replicase genes. In this presentation, resistance rates, verification of gene introduction in resistant plants, stability of resistance through generations, characteristics of viral multiplication and translocation in resistant plants, and resistance responses relative to inoculum potential and to various PVY strains will be shown. Yield and quality of leaf tobacco of a promising resistant tobacco line will be presented.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.6
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• pp.712-721
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• 1997

The objectives of this study were; (1) to identify and localize QTLs for resistance to soybean cyst nematode(SCN) race 5 on RAPD map, (2) to idntify the magnitude and mode of inheritance for each QTL, and (3) to identify the best combinations of QTLs for resistance to SCN race 5. Based on the univariate regression analysis, we detected 26 markers(22 RAPD and 4 RFLP) which showed significant association(P<0.05) with resistance to SCN race 5. From MAPMAKER /QTL analysis, we identified two regions (LGC-20 and Group 2) for resistance to SCN race 5. The QTL that was localized at 8.0 cM from pK418C on LGC-20 showed a recessive mode of inheritance and the QTL that was localized between W03 and E02$^3$ on Group 2 showed a dominant mode of inheritance. Two pairs of flanking markers (E02$^3$ and W03, pK418C and pK418E$_1$) and one unlinked RAPD marker, G10$^1$ were used for multiple regression analysis. Marker combination which was composed of 4 markers, E02$^3$, G10$^1$, W03, and pK418E$_1$, explained the highest amount of phenotypic variation by SCN (35.2％). Further research for the identification of QTLs for resistance to SCN race 5 to explain larger portion of phenotypic variation is needed.

• The Science & Technology
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• v.34 no.3 s.382
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• pp.78-79
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• 2001

전남대 잡초학연구실은 유전공학기술을 이용하여 수확량을 최고 26%까지 증산할 수 있는 다수확성 벼를 개발하는데 성공하여 화제를 모으고 있다. 이번에 개발된 다수확 형질전환 벼가 상용화되면서 현재 5백만톤 수준의 우리나라 벼 생산량이 7백만톤까지 늘어나게 된다. 구자옥교수가 1978년 부임하면서 개설된 잡초학연구실은 제초제연구, 제초제 저항성 잡초종의 생리생태에 대한 연구, 제초제 저항성 벼품종 개발사업 등을 진행하고 있다.

• Horticultural Science & Technology
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• v.32 no.6
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• pp.745-752
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• 2014

Chinese cabbage (Brassica rapa L. ssp. pekinensis) is an economically important vegetable crop as a source of the traditional food Kimchi in Korea. Although many varieties exhibiting desirable traits have been developed by the conventional selective breeding approach, breeding related to abiotic or biotic stresses, such as a particular pests or diseases, or tolerance to climatic conditions, is likely to be slow. This could be helped by an efficient method for selection from various, rapidly-evolved genetic resources on the basis of molecular markers. In particular, the Brassica genome sequencing project enables genome-wide discovery of genes or genetic variants associated with agricultural traits. We here discuss the recent progress in the field of Chinese cabbage breeding with regard to the application of molecular markers.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.65 no.3
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• pp.172-181
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• 2020

Koshihikari has been one of the most popular rice cultivars with good eating quality since the 1960s despite its susceptibility to blast disease and lodging. To map the genes controlling blast resistance and to develop promising blast-resistant breeding lines inheriting Koshihikari's high eating quality, a recombinant inbred line (RIL) population was developed from a cross between Koshihikari and a blast resistance donor with early maturity, Baegilmi. A total of 394 Koshihikari × Baegilmi RILs (KBRIL), and the two parents, were evaluated for blast resistance and major agronomic traits including heading date, culm length, panicle length, and tiller number. A linkage map encompassing 1,272.7 cM was constructed from a subset of the KBRIL (n = 142) using 130 single nucleotide polymorphisms. Two quantitative trait loci (QTL) for blast resistance, qBL1.1 harboring Pish/Pi35 and qBL2.1 harboring Pib, were mapped onto chromosomes 1 and 2, respectively. qBL1.1 was detected in both of the experimental sites, Namwon and Jeonju, while qBL2.1 was only detected in Namwon. qBL1.1 and qBL2.1 did not affect agronomic traits, including heading date, culm length, panicle length, and tiller number. From the 394 KBRILs, lines that were phenotypically similar to Koshihikari were selected according to heading date and culm length and were further divided into the following two groups based on blast resistance: Koshishikari-type blast resistant lines (KR, n = 15) and Koshishikari-type blast susceptible lines (KS, n = 15). Although no significant differences were observed in the major agronomic traits between the two groups, the KR group produced a greater mean head rice ratio than the KS group. The present study provides useful materials for developing blast-resistant cultivars that inherit both Koshihikari's high eating quality and Baegilmi's blast resistance.

• Korean journal of applied entomology
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• v.33 no.4
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• pp.230-236
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• 1994

Dicofol susceptible two-spotted spider mite(S) which was selected by 150 times of dicofol treatment showed 27 5-fold increase in resistance as compared to S strain. Inheritance of resistance and crossresistance in selected strain($R_d$) were investigated. There were differences oi susceptibility in the dicofol concentmtion-moltaliity relationships between $F_1$ progenies (R♀, XS♂, R♂, XS♀) obtained from reciprocal cross with the S strain and $R_d$, strain. Degree of dominance of the $F_1$ progenies (R♀, XS♂, R♂, XS♀) were -0.29 and -0.72 in adult females and -0.42 and -0.93 in adult males, respectively. These results suggest that inheritance of dicofol resistance is controlled by an incomplete recessive. $R_d$ strain exhibited a high level of cross resistance to amitraz, and also showed cross resistance to pyrethrotd acaricides, acrinathrin and bifenthnn. $R_d$ strain showed non-cross reslstancz to abamectin, chlolfenson, clofentezine, cyhexatm, fenbutatin oxlde, fenpyroximate, hexythiazox, monocrotophos, propargite and tetradifon, but negatively correlated cross-resistance to azocyclotin and fenbutatin oxide belonging to tin acaricides.

• Research in Plant Disease
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• v.18 no.4
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• pp.317-323
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• 2012

The study aims to identify the pathogenicity of Phytophthora. capsici isolates in major pepper-producing areas in Korea and the inherit genetic pattern of phytophthora blight resistance by inocula. With five kinds of testing materials including 'Kataguma (Sakata Korea)' peppers, a disease-susceptible material, '#308', a phytophthora blight resistance material, 'CM334', and their $F_1$ and $F_2$, respective isolates of P. capsici obtained from Icheon, Eumseong, Buan, Imsil and Yeongyang regions together with six kinds of peppers' inoculum including PA-159 (KACC No.40482) received from Korean Agricultural Culture Collection were used for inoculation. The disease-susceptible material '#308', the resistant material 'CM334' and the non-segregating generation of $F_1$ represented 4.94-5.00, 1.00-1.07, and 1.01-1.08 phytophthora blight incidence respectively in the group comparison by isolate. This result means that the phytophthora blight resistance was clearly distinguished among testing materials in the group comparison by P. capsici isolate. Moreover, $F_2$ segregating generation showed 1.79-2.31 phytophthora blight incidence which turned out to be identical in the group comparison by the six isolates of P. capsici isolate and with similarity between both the resistant and susceptible materials. Thus, the result proved that using the six isolates of P. capsici tested as inocula was suitable to investigate the phytophthora blight resistance. When it comes to group comparison of $F_2$ segregation generation, however, isolates were divided with PA-159 isolate being the center: a group consisting of isolates from Icheon, Buan, and Imsil and a group consisting of Yeongyang and Eumseong isolates with higher pathogenicity. The expected segregation ratio of the phytophthora blight resistance in $F_2$ generation by isolate was analyzed. PA-159 isolate showed 3:1 or 9:3:3:1, indicating that one to two genes were involved. On the other hand, results also proved that there is an interaction of genes since both Eumseong and Yeongyang isolates showed a segregation ratio of 11:5 while the Icheon isolate represented 12:3:1.