• KOREAN JOURNAL OF CROP SCIENCE
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• v.35 no.3
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• pp.201-210
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• 1990

This study was carried out to determine the effect of mixture of hymexazole and metalaxyl(Tachigarace) on the growth and reducing low temperature injury in rice seedlings. The amount of mixture of hymexazole and metalaxyl (Tachigarace) applied to reduce low temperature injury was 0, 9, 18 and 36mgr per pot (5${\times}$15${\times}$10cm) by soil incorperation befor sowing. Aging seedling was investigated on the growth responses by the application of 0.36gr mixture of hymexazole and metalaxyl (Tachigarace) per tray (30${\times}$60${\times}$3cm) after cutting of leaf at 35 day-old seedling. Rice seedlings treated with mixture of hymexazole and metalaxyl (Tachigarace) were better in the growth of top and roots, dry weight, erecting and rooting abilities than untreated seedlings. The effective amount of mixture of hymexazole and metalaxyl (Tachigarace) to increase erecting and rooting abilities was 18 mgr per pot. Under low temperature treatment, 12$^{\circ}C$, the leaf discoloration and dead leaf percent of rice seedlings were markedly decreased in the seedlings treated with mixture of hymexazole and metalaxyl (Tachigarace) as compared with untreated seedlings. The more amount of mixture of hymexazole and metalaxyl (Tachigarace) upto 36mgr per pot, the more effective on the low temperature injury in the seedling. The effect of mixture of hymexazole and metalaxyl (Tachigarace) to reduce low temperature damage in rice seedlings was mainly due to decreasing less physiological activities on the expanding leaf area and rooting, transpiration, stomatal diffusion and chlorophyll content of the leaves and oxygen consumption of the roots. Plant height, top dry weight and leaf area of aging seedlings were increased with mixture of hymexazole and metalaxyl (Tachigarace) treatment and leaf cutting as compared with untreated seedling and intact leaf, respectively. The ratio of missing hill of aging seedling was increased with increasing the period of growing seedling and was decreased due to leaf cutting, whereas the effect of mixture of hymexazole and metalaxyl (Tachigarace) application was not observed. Rooting ability of aging seedlings was decreased with lengthening of the period of raised seedling and was increased with mixture of hymexazole and metalaxyl (Tachigarace) treatment and leaf cutting.

• Journal of Life Science
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• v.21 no.7
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• pp.953-960
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• 2011

Necrosis is characterized by the cell membrane rupture and release of the cellular contents, including high-mobility group box 1 protein (HMGB1), into the extracellular microenvironment. HMGB1 acts as a transcriptional regulator in nuclei, but exerts a pro-inflammatory and tumor-promoting cytokine activity when released into the extracellular space. Its overexpression is associated with tumor progression and chemoresistance. Thus, HMGB1 acts as a clinically important molecule in tumor biology. In this study, we examined whether HMGB1 affects cell death induced by anti-cancer drugs. Here we show that HMGB1 prevented cisplatin (alkylating agent)-induced apoptosis and switched the cell fate to necrosis in MCF-7, MDA-MB231, and MDA-MB361 cells. Similar apoptosis-to-necrosis switch effects of HMGB1 were observed in cells treated with 4-HC, another alkylating agent. In contrast, HMGB1 did not exert any significant effects on docetaxel (DOC)-induced apoptosis in MCF-7 cells. We also show that cisplatin-induced apoptosis was switched to necrosis in MCF-7 multicellular tumor spheroids (MTS) that were cultured for 8 days and had necrotic cores, but DOC-induced apoptosis was prevented without the apoptosis-to-necrosis switch. Finally, the levels of RAGE, a receptor of HMGB1, were increased with extended culture of MTS. These findings demonstrate that HMGB1 switches alkylating agent-induced apoptosis to necrosis, suggesting that the strategy to prevent necrosis occurring as an undesirable action of alkylating agent-based chemotherapy should be delineated to improve the efficacy of chemotherapy for cancer.

• Journal of the Korean Society of Food Science and Nutrition
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• v.38 no.11
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• pp.1528-1534
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• 2009

This study was designed to evaluate the anti-obesity and hypoglycemic effects of Gugija (Lycium chinense Mill) extracts in 3T3-L1 adipocytes. We investigated the $\alpha$-amylase and $\alpha$-glucosidase inhibitory activities of extracts from Gugija. Gugija was extracted by 70% EtOH and 80% MeOH and aqueous, respectively. A single oral dose of Gugija extract inhibited the increase of blood glucose levels significantly at 0, 30, 60, 90 and 120 min and decreased incremental response areas under the glycemic response curve. These results suggest that Gugija 70% EtOH extracts may delay carbohydrate digestion and reduce postprandial hyperglycemia. In addition, triglyceride content in 3T3-L1 adipocytes decreased at higher concentrations of Gugija 70% EtOH extract. Free fatty acid content in 3T3-L1 adipocytes was increased at higher concentrations of Gugija 70% EtOH extract. Also, glucose transporter 4 (GLUT4), the key insulin signaling pathway transcription factor, was remarkably increased by the Gugija 70% EtOH extract when compared to those of control cells in protein expression levels. Therefore, Gugija can be developed as an effective anti-obesity and hypoglycemic agent.

• Journal of Life Science
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• v.26 no.5
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• pp.519-530
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• 2016

Vegetative plants in Yakutia are naturally frozen when they are covered with snow in the fall, and they function as green cryo-fodder that is a source of biologically active substances and nutrients for herbivorous animals. We observed a considerable increase in the total fatty acid content in the leaves of Avena sativa, Elytrigia rеpens, Equisetum variegatum and Equisetum scirpoides during the fall period. However, the degree of unsaturation of fatty acids was not higher in the frozen plants covered with snow than in the summer plants, with the exception of E. scirpoides, a dwarf horsetail found in the Pole of Cold in the northern hemisphere. In the internal adipose tissue of the Yakut horse (young horse meat), 18 fatty acids were found, including 10 saturated ones. Monounsaturated oleic С18:1 (n-9) acid and polyunsaturated α-linolenic С18:3 (n-3) acid were equally prevalent among the unsaturated fatty acids, accounting for 70% of the total unsaturated fatty acids. This composition of polyenoic fatty acids in the internal adipose tissue indicates that the Yakut horse actively feeds on the fall vegetation and the wintergreen sedge-grass. We believe that the high plant-specific free fatty acid content in the tissue of Yakut horses may play an important role in the regulation of their resistance to long-term low-temperature stress.

• Korean Journal of Soil Science and Fertilizer
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• v.16 no.4
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• pp.358-367
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• 1983

Antibiotic-resistant strains of Salmonella typhimurium and Klebsiella pneumoniae died readily after their addition to raw sewage, but they grew in sterilized sewage. The decline was not a result of antibiotic stresses, and because the bacteria were able to survive in large numbers for at least 15 days in solutions containing no organic nutrients, it was not a result of competition. Toxin production, bacteriophages, and Bdellovibrio did not cause the disappearance of the two bacterial species. A decline was also evident if the sewage was first passed through a $3-{\mu}m$ filter or treated with cycloheximide or cycloheximide plus nystatin, but protozoa developed under these conditions. Little or no decline occurred if the sewage was filtered and treated with the eucaryotic inhibitors before adding S. typhimurium or K. pneumoniae, and protozoa were not detected. S. typhimurium increased in abundance if cycloheximide, streptomycin, and erythromycin or large amounts of glucose were added to sewage. Tetrahymena thermophilus did not significantly reduce the population of S. typhimurium in buffer when the density of the bacterium was about $10^4/ml$. However, when more than $10^8$ Enterobacter agglomerans cells per ml were added to the buffer, T. thermophilus reduced the abundance of E. agglomerans and S. typhimurium to $10^6$ and 10/ml, respectively. The density of S. typhimurium was further decreased by a second increment of E. agglomerans cells. The disappearance of S. typhimurium and K. pneumoniae from sewage thus is the result of predation by protozoa. It is proposed that predators will eliminate a prey species from a natural environment when an alternate prey is present at concentrations above the threshold number for active feeing by the predator and when the rate of growth of the prey is less than the rate of predation.

• Korean journal of applied entomology
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• v.47 no.2
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• pp.155-162
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• 2008

The Welsh onion beet armyworm, Spodoptera exigua (Hubner), has attacked Welsh onion and is now the most important pest of Welsh onion in southwestern Korea. The beet armyworm has a wide host range, occurring as a serious pest of vegetable and fields. The relatively high abundance of beet armyworm has stimulated frequent application of insecticides to foliage. Insecticide resistance is a major problem in management of this insect. Accordingly, pesticide application for the control of beet armyworm was tried in both the open field and in laboratory, using 4 synthetic compounds such as metaflumizone and chlorfenapyr, indoxacarb, flufenoxuron, emamectin benzoate. In the laboratory, each developmental stages from eggs, larva to pupa was tested against 4 insecticides. Against the eggs of welsh onion beet armyworm, there was no significantly different with each other. These tested chemicals no killing effect to eggs. However, the population of 1st larva hatched from eggs were reduced because they eaten the egg shell with residual insecticides. The tested insecticides were taken very high mortalities to 1st to 3rd larva of Welsh onion beet armyworm. Otherwise, there were decreased the death rate from 4th to 6th larva. On the other hand, their value of control effects were relatively good against Welsh onion beet armyworms in the field between 87.2 and 90.5% on 10 days after insecticide application.