• Title/Summary/Keyword: 저장용액

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Comparison of Three Cardioplegic Solutions for Prolonged Cardiac Preservation During Ischemia in Korean Mongrel Dogs (한국산 잡견에서 허혈시 장시간심근보존을 위한 각 심정지액간 효과의 차이)

  • 조용길;류지윤
    • Journal of Chest Surgery
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    • v.29 no.10
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    • pp.1066-1075
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    • 1996
  • To compare the efficacy of cardiac preservation, we examined purine metabolites during 24 hours of cold storage($0^{\circ}C$) of the Korean ongrel dog hearts after using three different types of cardioplegic solutions. The hypothermic arrest with total cardiopulmonAry bypass method was employed in 51. Thomas solution(575) and blood cardioplegic solution(BCPS) preservation cases. Specimens were analyzed for levels of adenine nucleotides and their precursors by high performance liquid chromatography. The ATP content in the UW(University of Wisconsin) solution group tends to be higher than that of the combined hypothermic arrest group(575 and BCPS groups) after 2,4,8, and 12 hours of preservation respectively, but there were no significant differences between 575 and BCPS groups. The ADP contents in the UWS and BCPS groups were higher than that of the 575 group at 4,8, 12, and 24 hours, but the difference was not statistically significant between UWS and BCPS groups. The AMP contents did not change significantly in the three groups. The adenosine, Inosine, and hypoxanthine concentrations increased progressively, but the lev l of xanthine was very low in the three groups.

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The Effects of Calcinated Calcium Solution Washing and Heat Treatment on the Storage Quality and Microbial Growth of Fresh-cut Broccoli (신선편이 브로콜리의 품질과 미생물 성장에 영향을 주는 소성칼슘 용액 세척 및 열처리 효과)

  • Kim, Ji Gang;Nimitkeatkai, Hataitip;Choi, Ji Woen;Lee, Sang Gyu
    • Journal of Bio-Environment Control
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    • v.21 no.4
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    • pp.411-418
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    • 2012
  • This study was conducted to investigate the effect of calcinated calcium (CC) alone or combination with heat treatment on storage quality and microbial growth in fresh-cut broccoli. Fresh broccoli samples were cut into small pieces and washed in normal tap water (TW), $50{\mu}L{\cdot}L^{-1}$ chlorinated water (pH 6.5), $1.5g{\cdot}L^{-1}$ CC, heat treatment in TW at $45^{\circ}C$, and CC dissolved in TW at $45^{\circ}C$ for 2 minutes separately. Samples were then packaged in $50{\mu}m$ polyethylene bags and stored at $5^{\circ}C$. Results revealed that like $50{\mu}L{\cdot}L^{-1}$ chlorine, washing in CC at normal water temperature was effective in reducing microbial population in fresh-cut broccoli samples. Washing with CC combined with heat treatment increased an electrical conductivity of fresh-cut broccoli. Combined heat treatments with TW and CC reduced aerobic plate count on fresh-cut broccoli, only in initial period of storage. But, later on heat treatment induced injury of fresh-cut broccoli resulting more microbial population compared to non heat treatment. However, samples treated with CC alone had good quality with low off-odor at the end of storage. Results suggest that CC, an environment-friendly sanitizer could be an alternative to chlorinated water for washing of fresh-cut broccoli without affecting sensorial quality.

Effects of Medicinal Herb Extracts and Heat and Coating Treatments on the Browning Degree of Fresh-Cut Apples (한약재 추출물, 가열 및 코팅 처리가 신선절단 사과의 갈변도에 미치는 영향)

  • Chung, Hun-Sik;Lee, Joo-Baek;Moon, Kwang-Deog
    • Food Science and Preservation
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    • v.19 no.6
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    • pp.813-817
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    • 2012
  • The effects of dip treatments of chemicals (ascorbic acid, cystein, sodium chloride, magnesium chloride, calcium chloride, histidine: 1% solution) and medicinal herbs (Pueraria thunbergiana, Angelica gigas, Dioscorea japonica, Lindera strychifolia, Cnidium officinale, Astragalus membranaceus: 1% extracts), heat shock ($40-60^{\circ}C$ water), and edible coating (albumin, dextrin, sucrose poly ester, whole soy flour: 0.5-4% solution) on the browning degree of fresh-cut 'Fuji' apples were studied. Among the each treatment methods, the surface browning of fresh-cut apples was best retarded by calcium chloride dip, Astragalus membranaceus dip, $45-50^{\circ}C$ heat shock, coating with 3% dextrin or 1% whole soy flour. The results suggest that the treatments of medicinal herbs extracts, heat shock, and edible coating can be used as alternative for the use of chemical agents for the antibrowning of fresh-cut apples.

Prevention from microbial post-harvest injury of fruits and vegetables by using grapefruit seed extract, a natural antimicrobial agent (천연항균제처리에 의한 과채류의 선도유지 및 병해방지에 관한 연구 -저장중 병리적 장해 방지를 중심으로-)

  • Cho, Sung-Hwan;Seo, Il-Won;Lee, Keun-Hoi
    • Applied Biological Chemistry
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    • v.36 no.4
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    • pp.265-270
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    • 1993
  • In order to retain the freshness of fruits and vegetables and to reduce the rate of disease damage, grafruit seed extract (GPSE), natural microorganism control agent, was applied during the preservation process of fresh fruits and vegetables. GFSE showed an effective inhibitory action against plant putrefactive bacteria and fungi which were involved in the decay of fruits and vegetables. Minimal inhibitory concentrations for GFSE against the microbes were in the range of 50 to 2,000 ppm. Direct observation of microbial cells and spores using electron microscopy showed their function was destroyed by the treatment of the dilute solutions of GFSE. Fresh Welsh onions, onions and red peppers treated with GFSE and stored in polyethylene film (0.1 mm) retained better quality in color and texture than the non-treated control. GFSE was efficient in controlling the germination of potatoes. It was observed that GFSE would reduce disease damages and have bactericidal and fungicidal properties during the storage of such fruits and vegetables as zucchinis, cucumbers, tomatoes and mandarin oranges.

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Comparative analysis of various corrosive environmental conditions for NiTi rotary files (니켈티타늄 파일의 부식에 영향을 미치는 다양한 환경 조건 비교)

  • Yum, Ji-Wan;Park, Jeong-Kil;Hur, Bock;Kim, Hyeon-Cheol
    • Restorative Dentistry and Endodontics
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    • v.33 no.4
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    • pp.377-388
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    • 2008
  • The aim of the present study is to compare the corrosion tendency using two kinds of NiTi files in the various environmental conditions through the visual examination and electrochemical analysis. ProTaper Universal S2, 21 mm (Dentsply Maillefer, Ballaigues, Switzerland) and Hero 642, 0.06 tapers, size 25, 21 mm (Micromega, Besancon, France) rotary instruments were tested. The instruments were randomly divided into eighteen groups (n = 5) by the immersion temperature, the type of solution, the brand of NiTi rotary instrument and the presence of mechanical loading. Each file was examined at various magnifications using Scanning Electron Microscope (JEOL, Akishima, Tokyo, Japan) equipped with energy dispersive X-ray microanalysis (EDX). EDX was used to determine the components of the endodontic file alloy in corroded and noncorroded areas. The corrosion resistance of unused and used NiTi files after repeated uses in the human teeth was evaluated electrochemically by potentiodynamic polarization test using a potentiostat (Applied Corrosion Monitoring, Cark-in-Cartmel, UK). Solution temperature and chloride ion concentration may affect on passivity of NiTi files. Under the conditions of this in vitro study, the corrosion resistance is slightly increased after clinical use.

The Physical Properties of Port Type Crack Injection Method Using Latex Elastic Storage Tube (라텍스 탄성 저장관을 활용한 포트식 균열주입 공법의 물리적 특성에 관한 연구)

  • Kim, Eun-Young;Sho, Kwang-Ho
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.22 no.2
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    • pp.68-75
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    • 2018
  • In this study, to solve the problems of the conventional crack repair and injection method, high elasticity latex was used as the material of the storage tube to withstand the high pressure in the center part differently from the general port. When the repair solution was injected into the crack part, The TPS method was developed so that the air existing in the TPS can be discharged. In addition, a new infusion port in which a valve blocking the backflow of the remediation solution was installed at the injection port was developed and the physical characteristics of the port were analyzed. As a result of the evaluation, it was found that the filling rate of the remedial solution was improved compared to the existing ordinary injector method, and the cracks were completely filled in the test conditions. Compressive strength and tensile strength after repair showed about 20% decrease after repair in case of using ordinary injector method, while TPS method showed about 2~7% increase after repair. The results of this study showed that the injection port method using the elastic storage tube increased the injection performance and the quality after repair compared to the conventional injector method. The result of this study is expected to be utilized as the basic data for application and commercialization of the result to the practical structure.

Surfactant Enhanced In-Situ Soil Flushing Pilot Test for the Soil and Groundwater Remediation in an Oil Contaminated Site (계면활성제 원위치 토양 세정법을 이용한 유류 오염 지역 토양.지하수 정화 실증 시험)

  • 이민희;정상용;최상일;강동환;김민철
    • Journal of Soil and Groundwater Environment
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    • v.7 no.4
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    • pp.77-86
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    • 2002
  • Surfactant enhanced in-situ soil flushing was performed to remediate the soil and groundwater at an oil contaminated site, where had been used as a military vehicle repair area for 40 years. A section from the contaminated site (4.5 m $\times$ 4.5 m $\times$ 6.0 m) was selected for the research, which was composed of heterogeneous sandy and silt-sandy soils with average $K_d$ of 2.0$\times$$10^{-4}$cm/sec. Two percent of sorbitan monooleate (POE 20) and 0.07% of iso-prophyl alcohol were mixed for the surfactant solution and 3 pore volumes of surfactant solution were injected to remove oil from the contaminated section. Four injection wells and two extraction wells were built in the section to flush surfactant solution. Water samples taken from extraction wells and the storage tank were analyzed on a gas-chromatography (GC) for TPH concentration in the effluent with different time. Five pore volumes of solution were extracted while TPH concentration in soil and groundwater at the section were below the Waste Water Discharge Limit (WWDL). The effluent TPH concentration from wells with only water flushing was below 10 ppm. However, the effluent concentration using surfactant solution flushing increased to 1751 ppm, which was more than 170 times compared with the concentration with only water flushing. Total 18.5 kg of oil (TPH) was removed from the soil and groundwater at the section. The concentration of heavy metals in the effluent solution also increased with the increase of TPH concentration, suggesting that the surfactant enhanced in-situ flushing be available to remove not only oil but heavy metals from contaminated sites. The removal efficiency of surfactant enhanced in-situ flushing was investigated at the real contaminated site in Korea. Results suggest that in-situ soil flushing could be a successful process to remediate contaminated sites distributed in Korea.

Characteristics of Seed Germination and Promotion of Germination Rate in Pollia japonica Thunb. (나도생강의 종자 발아 특성과 발아율 향상)

  • Ro, Na-Young;Song, Eun-Young;Kim, Seong-Cheol;Jang, Ki-Chang;Moon, Doo-Young;Kang, Kyeung-Hee
    • Korean Journal of Plant Resources
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    • v.21 no.2
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    • pp.144-147
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    • 2008
  • This study was conducted to clarify the characteristics of seed germination and to promote the germination rate in Pollia japonica Thunb. that was pointed to protect by the Office of Forestry in 1997. It was better the germination rate and the growth of in the light than those of in the darkness. The seed germination of Pollia japonica showed the highest germination rate at $20^{\circ}C$ any other treated temperature. To increase the germination rate of Pollia japonica, it was conducted priming treatments and storage treatments at $4^{\circ}C$. Priming treatments using 1% NaOH, 1% KOH for 30min were effective compared to control, but soaking of 100mg/L GA3 for 24 hours, 1% NaOCl for 30min were not useful. It was higher in wet storage than in dry storage. Wet storage of seeds showed a germination rate with 95.3% in 90-day treatment, which improved 55.3% than dry storage in 90-day.

Physicochemical properties of chitosans produced from shrimp shell under the different conditions and their influences on the properties of Kakdugi during storage (제조조건이 다른 새우껍질 chitosan의 물리.화학적 성질 및 깍두기의 보존성에 미치는 영향)

  • Kim, Kwang-Ok;Kang, Hyen-Jun
    • Journal of the Korean Society of Food Culture
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    • v.9 no.1
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    • pp.71-77
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    • 1994
  • This study was undertaken to investigate the physicochemical properties of chitosans produced from chitins which were prepared from shrimp shell under the 4 different conditions(HCl concentration : 1 N, 4 N and reaction temperature $0^{\circ}C,\;20^{\circ}C$). The effects of chitosan on the properties of Kakdugi during storage also were examined. Viscosity of chitosan was markedly different$(313.1{\sim}98.8\;cps)$ depending on the production conditon. Chitosan solution showed pseudoplastic property. The infrared(IR) spectrums of chitosans prepared under the conditions of this study were very simillar, and the degree of deacetylation of chitosan was relatively high$(92{\sim}96%)$ independent of the extraction conditions of chitin. As the storage period was extended, markedly lower pH and higher titratable acidity were resulted in all the groups. Throughout the storage period, pH and titratable acidity of kakdugies with chitosan were higher and lower, respectively than those of control. Viscosity of kakdugi juice was significantly different among the groups and as the storage period increased, the juice viscosity decreased. At the eighth day of storage, the juice of control group was more viscous than those of kakdugies containing chitosan. Throughout the storage period the numbers of total microorganisms and of microorgarnisms of Leuconostoc genus in control kakdugi tended to be higher and lower, respectively than those in kakdugies containing chitosan. The growth of Lac. plantarum was slightly lower in kakdugi containing chitosan C produced at low HCl concentration and high temperature compared to the others.

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Preparation and Shelf-life of Soybean Curd Coagulated by Fruit Juice of Schizandra chinensis RUPRECHT(Omija) and Prunus mume(maesil) (오미자즙과 매실즙을 이용한 두부제조 및 저장)

  • Jung, Gi-Tai;Ju, In-Ok;Choi, Joung-Sik;Hong, Jae-Sik
    • Korean Journal of Food Science and Technology
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    • v.32 no.5
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    • pp.1087-1092
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    • 2000
  • Traditional food soybean curd was prepared using the fresh fruit juice of Schizandra chinensis RUPRECHT(Omija) and Prunus mume(maesil), and investigated the optimal preparative conditions(water addition ratio, heating time of mashed soybean and fresh fruit juice concentration), physical properties, sensory evaluation and shelf-life. Soybean curd coagulated with 0.9% Omija juice showed the highest yield at $85^{\circ}C$, 12.5 times water addition and 5 min heating. For soybean curd coagulated with 1.5% Maesil juice showed the highest yield at $85^{\circ}C$, 10 times water addition and 5 min heating. The physical properties (hardness, adhesiveness, chewiness and gumminess) of soybean curd coagulated with juices of Omija and Maesil showed lower values for hardness, adhesiveness, chewiness and gumminess. However sensory scores of both were evaluated higher than those of coagulated with $CaSO_4$. The shelf-life of soybean curd prepared from Omija and Masil juices and then soaked in 0.1% acetic acid was better than that of coagulated with $CaSO_4$ or soaked in distilled water.

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