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Isolation and characterization of a monodehydroascorbate reductase gene in poplar (Populus alba × P. glandulosa) (현사시나무 monodehydroascorbate reductase (MDHAR) 유전자의 분리 및 발현특성)

  • Yoon, Seo-Kyung;Park, Eung-Jun;Bae, Eun-Kyung;Choi, Young-Im;Kim, Joon-Hyeok;Lee, Hyoshin
    • Journal of Plant Biotechnology
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    • v.41 no.4
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    • pp.194-200
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    • 2014
  • Monodehydroascorbate reductase (MDHAR) is an important enzyme that plays a role in the detoxification of reactive oxygen species (ROS) by maintaining reduced pool of ascorbate through recycling the oxidized form of ascorbate. In this study, we isolated a PagMDHAR1 gene from Populus alba ${\times}$ P. glandulosa, and investigated its expression characteristics. The PagMDHAR1 cDNA encodes a putative 434 amino acids containing FAD- and NAD(P)H-binding domains. Southern blot analysis indicated that a single nuclear gene encodes this enzyme. Northern hybridization analysis revealed that PagMDHAR1 is highly expressed in both suspension cells and flower tissues, while its expression levels were enhanced by drought, salt, cold, wounding and ABA. Therefore, PagMDHAR1 might be expressed in response to abiotic stress through the ABA-mediated signaling pathway in this poplar species, suggesting that the PagMDHAR1 plays an important role in the defense mechanisms against oxidative stress.

Effect of Different Sowing Dates on the Yield-related Traits and Seed Yield of Sesame (파종시기에 따른 참깨 수량구성요소 및 수량에 미치는 영향)

  • Shim, Kang Bo;Shin, Myoung Na;Jeon, Won Tae;Han, Arum
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.66 no.4
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    • pp.411-418
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    • 2021
  • The yield potential of sesame depends on genetic factor, environmental factor, genetic × environmental interaction, as well as agronomic factors such as sowing date, planting density or seed rate for sowing. The main aim of this study was to analyze the effects of different sowing dates on the yield-related traits and total seed weight of sesame. The analysis of variance revealed that different sowing dates affected sesame yield-related traits such as plant height, number of branch per plant, number of capsule per plant, 1,000-seed weight, and total seed weight. Early or late sowing date showed negative effect of yield-related sesame traits and total seed weight. Optimum sowing date for sesame in the middle region of Korean peninsula was May 10th, in which total seed yield recorded 90 kg per 10 are. Path-coefficient analysis revealed that the number of capsule per plant and plant height were major factors that affected the total seed weight of sesame across different sowing dates.

Synthesis and Characterization of Collagen Peptide Based Copolymer from Shaving Scrap (셰이빙 스크랩으로부터 콜라겐 펩타이드계 공중합체 합성과 특성)

  • Park, Min Seok;Shin, Soo Beom;Kim, Ho Soo;Kim, Min Soo;Kim, Ha Sun;Jang, Jae Hyeok;Lee, Jin Kye;Lee, Dong Kuk
    • Applied Chemistry for Engineering
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    • v.33 no.6
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    • pp.581-587
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    • 2022
  • The leather industry generates a large amount of hazardous leather waste of various types every year. Among them, shaving scrap is difficult to recycle because it contains chromium ions. Many studies in recent years have shown that shaving scraps can be processed into various types of valuable products, such as adsorbent, filler, and poultry feed. In this study, collagen peptides were extracted from shaving scraps and structurally modified to be developed as new materials with improved physicochemical properties. First, the chromium ions contained in the shaving scraps were removed using a sodium hydroxide solution, and purified through concentration and low-temperature crystallization. The purified collagen peptide was used to prepare the powder using a spray dryer. The extracted collagen peptides were structurally modified by introducing double bonds by reacting with methacrylic anhydride (MAA), and the product was confirmed by 1H NMR spectroscopy. Next, a copolymer was prepared by redox polymerization of the modified collagen peptide (MCP) and 2-ethylhexyl acrylate (2-EHA). The structure of the copolymer was qualitatively confirmed by FT-IR. In conclusion, this study confirmed that collagen peptides can be extracted from shaving scrap and converted into new eco-friendly materials through certain treatments.

Characteristics and Control of Microthrix Parvicella Bulking in Biological Nutrient Removal Plant (생물학적 영양소제거공정에서 Microthrix Parvicella에 의한 Bulking 특성 및 제어)

  • Lee, H.;Ahn, K.
    • Journal of Korean Society on Water Environment
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    • v.22 no.6
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    • pp.1101-1106
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    • 2006
  • Many BNR (Biological Nutrient Removal) plants have experienced a bulking problem, mainly due to the growth of filamentous organisms, particularly during the winter months. This study investigated the problem of bulking due to the growth of M. parvicella both at a full-scale municipal wastewater treatment plant and a pilot scale plant located in the C city. The full-scale facility was operated at a flow rate of $51,000m^3/d$, an F/M (Food-to-Microorganism) ratio of 0.12 kgBOD/kgMLVSS/d and an SRT (Solids Retention Time) higher than 25 days, respectively. This plant experienced bulking and foaming problems at low temperatures below $15^{\circ}C$ since it was retrofitted with the BNR system in 2003. The pilot plant employed had an identical process configuration as the full scale one and used the same wastewater source. It was operated at a flow rate of $3.8m^3/d$, temperatures between 10 to $25^{\circ}C$ and SRTs between 10 and 25 days. At full scale, the M. parvicella growth and SVI (Sludge Volume Index) patterns were studied in conjunction with temperature variations. At pilot scale, DO and SRT variations were also explored, in addition to the filamentous bacteria growth and SVI patterns. During the full-scale investigation, over a 3 year period, it was noted that the SVI was maintained within acceptable operational values (i.e. under 160) during the summer months. Moreover settling in the secondary clarifiers was good and was not affected by the presence of M. parvicella. In contrast, at low mean temperatures during winter, the SVI increased to over 300. Overall, as the temperature decreased, the predominance of M. parvicella became apparent. According to this study, M. parvicella growth could be controlled and SVI could drop under 160 by a change in operational conditions which involved an increase in DO concentration between 2 and 4 mg/L and a decrease in SRT to less than 20 days.