• Journal of the korean veterinary medical association
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• v.47 no.1
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• pp.47-54
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• 2011

최근 몇년간 국립수의과학검역원에서 조사한 바에 따르면, 국내 젖소에서 유방염을 일으키는 주된 원인균은 황색포도상구균을 제외하고는 거의 대부분 젖소의 환경으로부터 유래하는 환경성 세균에 의한 것으로 확인된 바 있다. 국내 젖소의 유방염 원인균으로 작용하고 있는 이러한 환경성 세균 중 coagulase-음성 포도상구균 (Coagulase-negative Staphylococci, CNS)과 E. coli 등의 그람 음성균이 가장 많은 비율을 차지하였지만 그 외에 Streptococcus uberis를 비롯한 환경성 연쇄상 구균과 장구균도 상당한 비율을 차지하는 것으로 조사되었으며 이에 대해서는 지난 9월호에서도 상세히 기술한 바 있다. 환경성 연쇄상구균과 장구균은 젖소에서 유선감염 및 임상형 유방염의 중요한 원인이며 환경성 연쇄상구균 중 가장 흔히 발견되는 유방염 원인체는 Streptococcus uberis 로서, 이는 국내에서뿐만 아니라 미국, 유럽, 뉴질랜드, 호주 등에서 수행된 많은 연구결과에서도 마찬가지로 나타났다. 장구균 중 가장 흔히 분리되는 균종은 E. faecium과 E. faecalis로서 검역원에서 지난 몇 년간 조사된 결과에서도 이 두 가지 균 종이 유방염우유에서 분리된 장구균의 90% 이상을 차지하였다. 이러한 환경성 연쇄상구균과 장구균은 사람과 동물의 장관, 분변, 감염된 유방 및 전체적인 목장 환경에서 분리되고 있다. 장구균은 일반적으로 환경성 연쇄상구균/장구균 유방염 감염증 중의 일부일뿐이고 장구균에 의한 유방염이 목장의 주된 문제가 되는 일은 비교적 드물다. 현재, 장구균에 의해 발생하는 유방염은 S. uberis를 통제하기 위해 사용되는 방법과 동일한 방법으로 통제할 수 있다고 여겨진다. 원인체에 상관없이 유방염을 통제하기 위한 기본적인 원칙은 유방염을 일으킬 수 있는 병원체에 유두끝이 노출되는 기회를 줄이거나 감염에 대한 젖소의 저항성을 증가시키는 두 가지 방법이다. 역으로 얘기하면, 병원체에 대한 유두끝 노출을 증가시키거나 감염에 대한 젖소의 저항성을 감소시키는 요인들은 우군 내에서 더 많은 유방염을 발생시킬 가능성이 높기 때문에 위험요인으로 간주된다. 본 고에서는 환경성 연쇄상구균에 의한 유방염과 관련이 있는 것으로 알려진 이러한 여러 가지 위험요인들에 대해 알아보고 그러한 위험요인을 줄일 수 있는 방법에 대해서도 고찰하고자 한다.

• Korean Journal of Microbiology
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• v.52 no.1
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• pp.25-31
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• 2016

An enterococci selective medium was developed to detect the presence of enterococci for use as a fecal contamination indicator. Among several media which have been known to detect enterococci, the following 9 different kinds of media were selected: Enterococci Confirmatory agar, Azide dextrose agar, Bromocresol-purple azide agar, Esculin bile agar, Citrate azide tween carbonate agar, KF Streptococcus agar, BROLACIN agar, Kanamycin esculin azide agar, and Membrane filter Enterococcus selective agar. Various components from the nine media were mixed to develop a more effective enterococcus selective medium. The newly developed medium named as 'Enterococcus Mixed medium' was more effective than the previous 9 media. Enterococci strains (Enterococcus avium KACC 10788, Enterococcus faecium KACC 11954, Enterococcus saccharolyticus KACC 10783, Enterococcus durans KACC 10787, Enterococcus faecalis KACC 11304, and Enterococcus hirae KACC 10779) and non-enterococci strains (Escherichia coli KACC 10005, Staphylococcus aureus subsp. aureus KACC 10768, and Bacillus subtilis KACC 10111) were used to test the new medium. As a result, the enterococci strains grew well on the Enterococcus Mixed medium whereas the non-enterococci strains did not grow well on it. Additionally, growth of enterococci with freshwater and seawater samples was observed to be good on the Enterococcus Mixed medium. The result of this study confirmed that the Enterococcus Mixed medium was effective in detecting the target enterococci.

• Childhood Kidney Diseases
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• v.17 no.1
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• pp.19-24
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• 2013

Purpose: Recently, enterococcus spp. have become one of the most common nosocomial pathogens with increasing rates of multi-drug resistance. However, study on enterococcal urinary tract infections (UTIs) in children is very limited, especially community acquired UTIs. We studied the clinical characteristics of enterococcus spp. in community acquired UTIs and antibiotic resistance within our urban area. Methods: All children with first episode of community acquired UTIs due to enterococcus spp. and Echerichia coli who were admitted in Pusan National University Children's Hospital between January 2010 and January 2013 were included in our study. We retrospectively reviewed their medical records. Results: During the study period, 201 patients were identified to have first episode of community acquired UTIs. 154 cases were E.coli UTIs (76.6%) and 11 cases were enterococcal UTIs (5.5%) and all enterococcus spp. were Enterococcus feacalis. In enterococcal UTI group, voiding cystourethrogram(VCUG) was performed in 7/11 patients and demonstrated 4 vesicoureteral refluxes (VURs) with renal scar and 3 patients underwent corrective surgery. In E.coli UTI group, VCUG was performed in 121/154 patients and demonstrated 23 VURs and 11 patients underwent corrective surgery. Enterococcal group had significant high rate of underlying urinary abnormalities and surgical corrections compared with E. coli group. All enterococcus spp. were susceptible to ampicillin, vancomycin and linezolid, but all were resistant to tetracycline. They also showed 71.4% resistance to trimethoprim-sulfamethoxazole and 20% resistance to ciprofloxacin. Conclusion: Community acquired enterococcal UTIs in children were rare within our urban area. However, they could be indicative of severe underlying urinary tract abnormalities.

• Journal of Korean Society of Environmental Engineers
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• v.32 no.10
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• pp.979-985
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• 2010

This study was performed to detect enterococci strain as an indicator of faecal contamination, to identify of 16S rDNA sequence and vancomycin resistance by MIC (Minimum Inhibitory Concentration) test from drinking spring-water samples in Seoul. The detection frequency of enterococci was 42 (19.8%) among 212 samples, and its concentration was ranged from 0 to 110 CFU/100 mL. These results were confirmed the possibility as an indicator microorganisms that similar to the frequency of E. coli detection (t test p-value 0.268, significant level 0.05). Isolated 56 enterococci samples were identified by 16S rDNA sequence data and their NCBI BLAST searching. They were identified to Enterococcus faecalis of 24 samples, E. faecium (10), E. casseliflavus (10), E. gallinarum (3), E. hirae (2), E. durans (2), E. sanguinicola (1). E. faecalis was dominant species that clinical case report of a domestic was similar. Vancomycin resistant enterococci (VRE) of 53 samples showed that vanB and vanC1/C2 type with 2 and 12 case, respectively. These results indicated that the drinking spring-water quarantined to fecal pollution for block out outbreak of gastrointestinal symptom with using such as disinfection process.

• Proceedings of the Korea Water Resources Association Conference
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• 2015.05a
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• pp.162-167
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• 2015

매년 여름철 피서지 1순위로 손꼽히는 해수욕장은 해양레포츠의 발달과 관광사업의 촉진으로 수많은 관광객들이 방문하고 있다. 하지만 우리나라 대부분의 해수욕장에서는 건전한 해수욕을 위한 수질안전지표 설정이 매우 미흡한 편이며 오염수준 또한 심각하다. 이에 강원도 강릉시 경포해수욕장을 대상지역으로 2012년 7월 17일부터 9월 4일까지 매주 화요일 오전에 채취한 시료로부터 병원성 수질지표인 장구균과 대장균, 총대장균군의 오염도를 분석하였다. 시료는 경포해수욕장 오리바위 배후부터 연안방향을 따라 200m 간격으로 3지점과 이안방향을 따라 백사장(dry beach), 포말대(swash zone), 유영층(swimming zone)의 3지점에서 총 9개를 채취하였다. 기온, 강우, 장소 등의 해안 환경 변화에 따른 병원균 증식 유리조건 및 인자들간의 상관성을 분석한 결과 백사장에서는 총대장균군, 포말대에서는 장구균, 유영층에서는 대장균의 농도가 가장 높았다. 또한 주간 누적 강우량과 평균 기온에 따라 시료채취 지점마다의 상관성에서는 백사장은 강우량과 정비례, 기온과는 반비례, 포말대와 유영층은 강우량과 반비례, 기온과는 정비례의 관계를 보였다. 백사장과 해수층인 포말대, 백사장과 유영층 각각은 반비례 관계를 보였지만 미비한 수준이었으며 포말대와 유영층간의 상관성은 약 90%의 매우 높은 수치를 띄었다. 본 연구는 건전한 수계 레크레이션 문화의 수질 안전지표 설정을 위해 해수욕장의 대표 관광명소 중 하나인 경포해수욕장을 대상으로 병원성 수질 지표들의 증식 유리 조건 및 확산특성 예측에 목적이 있다. 나아가 본 과정은 해변을 찾는 관광객들의 수질 안전 정보 제공 및 체계적인 가이드라인 제시로도 가치 있을 것으로 기대된다.

• Journal of Food Hygiene and Safety
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• v.25 no.2
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• pp.122-128
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• 2010

From April to December in 2009, microbial investigation is accomplished for 100 frozen foods asked to microbial control team that corresponds with total aerobic viable bacteria, coliform group, Escherichia coli, Enterococcus spp. and antibiotic resistance patterns of Enterococcus spp. isolates are investigated. Average of total erobic viable bacteria numbers is $4.3{\times}10^4CFU/g$. Average of coliform group numbers is $4.3{\times}10^3CFU/g$. Average f Enterococcus spp. numbers is $1.8{\times}10^3CFU/g$. Escherichia coli from 100 frozen foods is not detected and detection ate is 0.0%. 22 Enterococcus spp. are isolated from 100 frozen foods. 12 of 22 Enterococcus spp. strains are identified as E. faecium. 7 of 22 Enterococcus spp. strains are identified as E. faecalis. 2 of 22 Enterococcus spp. trains are identified as E. gallinarum. 1 of 22 Enterococcus spp. strains is identified as E. hirae. Enterococcus spp. solates show a high resistance to erythromycin, rifampin, tetracycline, ciprofloxacin, chlorampenicol, penicillin and susceptibility to vancomycin, ampicillin, gentamicin, strepomycin, linezolid. 15 of 22 Enterococcus spp. strains are multi-resistant and the most frequent multi-resistant pattern is erythromycin-rifampin for 6 Enterococcus spp. strains.

• Proceedings of the Korea Water Resources Association Conference
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• 2006.05a
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• pp.2023-2026
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• 2006

• Korean Journal of Microbiology
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• v.46 no.2
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• pp.148-151
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• 2010

The aim of this study was to investigate the erythromycin resistance patterns of Enterococci sp. present in cow milk. A total 110 erythromycin resistant Enterococci were isolated from milk samples; E. faecalis (n=101), E. avium (n=7), and E. durans (n=2). The minimum inhibitory concentration of erythromycin against 110 Enterococci were determined. The 66.3% of Entercocci (n=73) showed high level resistance (${\geq}64$ mg/ml). Among 110 isolates, 86.3% (n=95) showed $cMLS_B$ phenotype and 13.6% (n=15) showed $iMLS_B$ The aim of this study was to investigate the erythromycin resistance patterns of Enterococci sp. present in cow milk. A total 110 erythromycin resistant Enterococci were isolated from milk samples; E. faecalis (n=101), E. avium (n=7), and E. durans (n=2). The minimum inhibitory concentration of erythromycin against 110 Enterococci were determined. The 66.3% of Entercocci (n=73) showed high level resistance (${\geq}64$ mg/ml). Among 110 isolates, 86.3% (n=95) showed $cMLS_B$ phenotype and 13.6% (n=15) showed $iMLS_B$ phenotype. All of isolates have erm(B) determinant, 75.45% (n=83) have mef(A) an efflux system determinant. The majority of Enetrcococci isolated from raw milk samples in northern area of Gyeonggi-Do showed high level of resistance to erythromycin.

• Journal of the korean veterinary medical association
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• v.40 no.7
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• pp.634-638
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• 2004

• Korean Journal of Fisheries and Aquatic Sciences
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• v.8 no.3
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• pp.157-165
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• 1975

This experiment was carried out to evaluate the sanitary quality of commercially frozen sea foods. One hundred and sixteen samples in six different items from several refrigeration plant in Busan city were examined from March to December in 1974. In addition, the changes in bacterial density through the process from thawing, round or semifilleted frozen alaska pollack to the finishing as frozen fillet blocks were observed. To evaluate the sanitary quality, sanitary indicative bacteria such as total coliform, fecal coliform, fecal streptococci and enterococci as well as plate counts were determined. From the results, the median value of fecal coliform MPN was 20 per 100 grams of the samples and that of enterococci was 790. The median value of plate counts was $2.2\times10^4$ per gram. The plate counts were not correlated with the number of sanitary indicative bacteria. The results suggest that enterococci could be used advantageously in preference to coliform organisms as indicative bacteria for the evaluation of sanitary quality of frozen sea foods. The plate counts at $20^{\circ}C$ of the samples were 14 times higher than that at $35^{\circ}C$. Geometric mean of total coliform MPN was 310 and that of enterococci was 143. Bacterial density was reduced by fleering. Morethan 50 percent for total coliform MPN and $35^{\circ}C$ plate counts, and about 35 percent for enterococci MPN and $20^{\circ}C$ plate counts were reduced under the contact freezing unit which was generally operated at $-40^{\circ}C$. About fifty-five percent of the samples were negative in fecal coliform test and 10 percent of those were exceeded $1.0\times10^5$ per gram in $35^{\circ}C$ plate counts.