Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Analysis of Genotype and Phenotype of Erythromycin Resistance in Enterococci spp. Isolated from Raw Milk Samples

원유시료에서 분리한 장구균의 에리스로마이신 내성 유전자형 및 표현형 분석

  • Received : 2010.04.20
  • Accepted : 2010.06.04
  • Published : 2010.06.30
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Abstract

The aim of this study was to investigate the erythromycin resistance patterns of Enterococci sp. present in cow milk. A total 110 erythromycin resistant Enterococci were isolated from milk samples; E. faecalis (n=101), E. avium (n=7), and E. durans (n=2). The minimum inhibitory concentration of erythromycin against 110 Enterococci were determined. The 66.3% of Entercocci (n=73) showed high level resistance (${\geq}64$ mg/ml). Among 110 isolates, 86.3% (n=95) showed $cMLS_B$ phenotype and 13.6% (n=15) showed $iMLS_B$ The aim of this study was to investigate the erythromycin resistance patterns of Enterococci sp. present in cow milk. A total 110 erythromycin resistant Enterococci were isolated from milk samples; E. faecalis (n=101), E. avium (n=7), and E. durans (n=2). The minimum inhibitory concentration of erythromycin against 110 Enterococci were determined. The 66.3% of Entercocci (n=73) showed high level resistance (${\geq}64$ mg/ml). Among 110 isolates, 86.3% (n=95) showed $cMLS_B$ phenotype and 13.6% (n=15) showed $iMLS_B$ phenotype. All of isolates have erm(B) determinant, 75.45% (n=83) have mef(A) an efflux system determinant. The majority of Enetrcococci isolated from raw milk samples in northern area of Gyeonggi-Do showed high level of resistance to erythromycin.

동물성 식품 유래 장구균의 에리스로마이신 내성 유전자형 및 표현형을 분석하고자 하였다. 경기북부지역의 축산 농가 15곳으로부터 원유 시료 378개를 분양받아 본실험에 사용하였으며 에리스로마이신 내성 장구균 분리에 사용하였다. 총 110균주의 장구균이 분리 되었으며 이중 E. faecalis가 101균주, E. avium이 7균주, E durans가 2균주였다. 에리스로마이신에 대한 내성 비율은 65.45%였으며 $MIC_{50}$은 16 ${\mu}g$/ml, $MIC_{90}$은 64 ${\mu}g$/ml이었다. 분리된 장구균 110균주 모두 erm(B) 유전자를 소유하고 있었으며 75.45%인 83균주가 mef(A)를 소유하고 있음을 확인하였다. 표현형 분석에 따르면 지속성 내성($cMLS_B$)을 나타내는 균주는 95균주(86.36%)였으며 유도내성 ($iMLS_B$)을 보이는 균주는 15균주(13.34%)였다.

Keywords

References

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