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Prediction of protein binding regions in RNA using random forest (Random forest를 이용한 RNA에서의 단백질 결합 영역 예측)

  • Choi, Daesik;Park, Byungkyu;Chae, Hanju;Lee, Wook;Han, Kyungsook
    • Proceedings of the Korea Information Processing Society Conference
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    • 2016.10a
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    • pp.583-586
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    • 2016
  • 단백질과 RNA의 상호작용 데이터가 대량으로 늘어남에 따라, 단백질과 RNA의 결합부위를 예측하는 계산학적인 방법들이 많이 개발되고 있다. 하지만, 많은 계산학적인 방법들은 단백질에서 단백질과 RNA 결합부위를 예측한다는 한계점이 있었다. 본 논문에서는 RNA와 단백질의 서열정보를 모두 사용하여, 단백질과 결합하는 RNA 결합부위를 예측하는 기법과 그 결과를 논한다. WEKA random forest(http://www.cs.waikato.ac.nz/ml/weka/)를 이용하여 예측 모델을 개발하였고, RNA 서열의 서열 프로파일, 서열 composition, 결합 상대방의 단백질의 특성 등을 특정으로 표현하였다. Random forest 기법을 사용한 cross validation의 결과로서 1:1 모델에서 제일 높은 성능인 92.4% sensitivity, 92.0% specificity, 92.2% accuracy를 보였고, independent test에서는 72.5% sensitivity, 90.0% specificity, 2.1% accuracy를 보였다.

축소 노즐에서의 슬롯 막냉각 열전달 특성에 관한 연구

  • 조용일;조형희
    • Proceedings of the Korean Society of Propulsion Engineers Conference
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    • 2000.04a
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    • pp.33-33
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    • 2000
  • 고온의 연소가스로부터 노즐 표면을 보호하기 위하여 슬롯을 통하여 냉각 유체를 분사하는 슬롯 막냉각에 대하여 연구하였다. 냉각효율 및 열전달 특성은 주유동과 2차 유동의 분사율에 따라 크게 달라지며, 형상변화 및 유동가속에 의해서도 냉각 효과의 변화를 가져오게 된다. 따라서 본 연구에서는 실험을 통하여 면적비가 16:1인 축소노즐에서 압축성 효과를 배제할 수 있는 유동속도 범위 내에서 분사율 변화(0.5 $\leq$ M $\leq$ 3.0)에 따른 슬롯 막냉각 열전달 특성을 고찰하고, 평판 슬롯 막냉각 경험식의 결과와 비교하였으며, 수치해석을 통하여 축소노즐과 원형관에서의 냉각효율 및 열전달 특성을 비교함으로서 이를 검증하였다. 축소노즐에서의 슬롯 막냉각 열전달 특성은 단열벽면조건을 형성하여 노즐 표면을 따라 설치된 열전대를 이용하여 측정하였다. 그 결과 상대적으로 낮은 분사율(M=0.5, 1.0)에서 분사율 증가에 따른 냉각효율의 증가가 크게 나타났으며, 분사율이 높아짐(M $\geq$ 2.0)에 따라 냉각효율의 증가폭이 점점 감소하고, 일정 분사율 이상에서는 냉각 효율의 증가가 크게 둔화되었다. 분사율이 낮을 경우 평판 슬롯 막냉각 경험식으로 주어진 결과보다 상류에서는 높으나 하류로 진행하면 비슷한 냉각효율을 보였고, 분사율이 높은 경우 평판보다 전 범위에서 약간 높은 냉각효율을 나타냈다. 수치해석 결과에서는 분사율이 낮을 경우 축소노즐의 냉각효율이 원형관에서의 냉각효율 보다 낮거나 비슷하게 나타났으며, 분사율이 높아짐에 따라 축소노즐에서의 냉각효율이 오히려 높아지는 것으로 나타났다.타내었다. 액체 제트의 속도는 처음에는 일정하게 유지되다가 운동량을 보존하기 위해 가스로부터 운동량을 받아 점차 가속되어지는 것으로 나타났다.본 규격은 키, 총장, 어깨길이, 등길이, 머리길이, 머리둘레, 진동둘레, 목둘레, 가슴둘레, 허리둘레, 배둘레, 엉덩이둘레, 앞품, 뒤품, drop치를 포함하고 있고, 각 규격에서 호칭간 치수 간격도 함께 제시하고 있다. 본 연구 결과에서 보듯, 현행 8규격의 무진복의 각 호칭간 적정 허용범위를 고려해 합리적인 치수체계를 정립한다면 치수에 대한 적합도가 상당히 증가할 뿐 아니라 생산비용도 상당히 감축할 것으로 생각된다.나타났다. 4) 호감적 서비스능력 차원에서 세 독립변수간에 유의한 3원 상호작용이 존재하는 것으로 나타나( $F_{2,228}$=15.62, P<.001) 20대에 적합한 의복 착용시( $F_{2,228}$=3.98, P<.05)와 60대에 적합한 의복 착용시( $F_{2,228}$=16.55, P<.001) 점포유형과 격식차림간에는 유의한 상호작용이 존재하는 것으로 나타났다. 5) 호감을 구성하는 세 요인들이 구매의도에 미치는 영향을 조사한 결과 호감적 인상차원은 29%(P<.001), 호감적 서비스능력차원은 6%(P<.001)의 구매의도를 설명해 주는 것으로 나타났다. 본 연구결과 노년 소비자에게 호감을 주는 판매원의 외모는 구매의도에 영향을 주어 실버의류산업의 이익증대와 밀접한 연관을 갖는 서비스품질의 중요한 요인으로 밝혀졌다.중요한 요인으로 밝혀졌다.로운 단백질 EPSPS가 다른 여러 식물에 이미 존재하고 있는 단백질로서 우리가 이미 이러

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Study on the Adsorption of Antibiotics Trimethoprim in Aqueous Solution by Activated Carbon Prepared from Waste Citrus Peel Using Box-Behnken Design (박스-벤켄 설계법을 이용한 폐감귤박 활성탄에 의한 수용액 중의 항생제 Trimethoprim의 흡착 연구)

  • Lee, Min-Gyu;Kam, Sang-Kyu
    • Korean Chemical Engineering Research
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    • v.56 no.4
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    • pp.568-576
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    • 2018
  • In order to investigate the adsorption characteristics of the antibiotics trimethoprim (TMP) by activated carbon (WCAC) prepared from waste citrus peel, the effects of operating parameters on the TMP adsorption were investigated by using a response surface methodology (RSM). Batch experiments were carried out according to a four-factor Box-Behnken experimental design with four input parameters : concentration ($X_1$: 50-150 mg/L), pH ($X_2$: 4-10), temperature ($X_3$: 293-323 K), adsorbent dose ($X_4$: 0.05-0.15 g). The experimental data were fitted to a second-order polynomial equation by the multiple regression analysis and examined using statistical methods. The significance of the independent variables and their interactions was assessed by ANOVA and t-test statistical techniques. Statistical results showed that concentration of TMP was the most effective parameter in comparison with others. The adsorption process can be well described by the pseudo-second order kinetic model. The experimental data of isotherm followed the Langmuir isotherm model. The maximum adsorption amount of TMP by WCAC calculated from the Langmuir isotherm model was 144.9 mg/g at 293 K.

Effects of Concentration of Inhibitor on the Production of $\alpha$-amylase and Growth of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens 성장과 $\alpha$-amylase 생합성에 미치는 저해제의 영향)

  • 김종균;김종수차월석
    • KSBB Journal
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    • v.11 no.2
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    • pp.125-131
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    • 1996
  • In this study, Bucillus amyloliquefaciens was adopted as bacterial source to investigate the concentration of carbon source by adding inhibitors in the batch culture. By adding acetic acid at $10g/\ell$ of initial glucose concentration, maximum dry cell density was obtained with the highest value of /$\3.9gell$ at $1.0g/\ell$ of initial acetic acid concentration. By adding acetic acid al 10g/$\ell$ of initial glucose concentration, maximum ${\alpha}$-amylase production was obtained with 331.55unit/m1 at $2.0g/\ell$ of initial acetic acid concentration. ${\alpha}$-Amylase production was decreased with the increase of initial acetic acrid concentration. By adding acetic acid to the medium, cell growth and ${\alpha}$-amylase production was higher in glucose than in maltose. By adding lactic acid to the medium, cell growth was decreased.

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Li-bearing Tosudite from the Sungsan Mine, Korea (해남 성산광산에서 산출되는 함리튬 토수다이트)

  • Cho, Hyen-Goo
    • Journal of the Mineralogical Society of Korea
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    • v.4 no.1
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    • pp.1-10
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    • 1991
  • Tosudite from the Sungsan mine, Korea is an alteration product of rhyolitic tuff in the cretaceous Hwangsan Formation. It is associated with illite, dickite, nacrite or quartz and also found in the cavities of black claystone. X-ray diffraction and chemical analyses show that the Sungsan tosudite is a lithium-bearing aluminous 1:1 regularly interstratified mineral of di, dioctahedral chlorite and smectite. Its structural formula is $(K_{0.73}Na_{0.02}Ca_{0.07})(Si1_{13.23}Al_{2.77})(Li_{0.52}Mg_{0.08}Mn_{0.01)Fe^{3+}_{0.07}Al_{12.33})O_{40}(OH)_{20}$ and it suggests that Sungsan tosudite consists of regularly interstratified Li-donbassite and beidellite. DTA and TG curves as well as IR absorption data also support such a result. Temperature of formation of tosudite is inferred to be between $110{\circ}$ and $270{\circ}C$.

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Optimization and Development of Prediction Model on the Removal Condition of Livestock Wastewater using a Response Surface Method in the Photo-Fenton Oxidation Process (Photo-Fenton 산화공정에서 반응표면분석법을 이용한 축산폐수의 COD 처리조건 최적화 및 예측식 수립)

  • Cho, Il-Hyoung;Chang, Soon-Woong;Lee, Si-Jin
    • Journal of Korean Society of Environmental Engineers
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    • v.30 no.6
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    • pp.642-652
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    • 2008
  • The aim of our research was to apply experimental design methodology in the optimization condition of Photo-Fenton oxidation of the residual livestock wastewater after the coagulation process. The reactions of Photo-Fenton oxidation were mathematically described as a function of parameters amount of Fe(II)($x_1$), $H_2O_2(x_2)$ and pH($x_3$) being modeled by the use of the Box-Behnken method, which was used for fitting 2nd order response surface models and was alternative to central composite designs. The application of RSM using the Box-Behnken method yielded the following regression equation, which is an empirical relationship between the removal(%) of livestock wastewater and test variables in coded unit: Y = 79.3 + 15.61x$_1$ - 7.31x$_2$ - 4.26x$_3$ - 18x$_1{^2}$ - 10x$_2{^2}$ - 11.9x$_3{^2}$ + 2.49x$_1$x$_2$ - 4.4x$_2$x$_3$ - 1.65x$_1$x$_3$. The model predicted also agreed with the experimentally observed result(R$^2$ = 0.96) The results show that the response of treatment removal(%) in Photo-Fenton oxidation of livestock wastewater were significantly affected by the synergistic effect of linear terms(Fe(II)($x_1$), $H_2O_2(x_2)$, pH(x$_3$)), whereas Fe(II) $\times$ Fe(II)(x$_1{^2}$), $H_2O_2$ $\times$ $H_2O_2$(x$_2{^2}$) and pH $\times$ pH(x$_3{^2}$) on the quadratic terms were significantly affected by the antagonistic effect. $H_2O_2$ $\times$ pH(x$_2$x$_3$) had also a antagonistic effect in the cross-product term. The estimated ridge of the expected maximum response and optimal conditions for Y using canonical analysis were 84 $\pm$ 0.95% and (Fe(II)(X$_1$) = 0.0146 mM, $H_2O_2$(X$_2$) = 0.0867 mM and pH(X$_3$) = 4.704, respectively. The optimal ratio of Fe/H$_2O_2$ was also 0.17 at the pH 4.7.

Studies on Strength of Netting (1) The Decrease in Strength of Netting Twines by Knotting (그물감의 강도에 관한 연구 (1) 그물실의 강도가 매듭에서 감소하는 기구)

  • KIM Dai An
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.9 no.1
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    • pp.1-7
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    • 1976
  • 1) The decrease in strength of netting twines at the knot may be regarded to be due mainly to the frictional force acting on the tip of the knot. The knot strength T may be given by $$T=\frac{T_0}{1+{\mu}\frac{s}{\rho}\varrho^{\mu\theta}$$ were $T_0$ is the tensile strength of unknotted netting twines, $\mu$ the coefficient of friction beween two netting twines forming a knot, s the contact length between the tip and the netting twine compressing it, $\rho$ the radius of curvature of the compressing, and $\theta$ the angle at which the compressing rubs with another one in the vicinity of the opposite tip. 2) Knots are arranged in order of strength as follows : the reef knot pulled lengthwise $\risingdotseq$ the trawler knot pulled breadtwise the reef knot pulled breadthwise.

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Adsorption Thermodynamics, Kinetics and Isosteric Heat of Adsorption of Rhodamin-B onto Granular Activated Carbon (입상 활성탄에 의한 Rhodamin-B의 흡착 열역학, 동력학 및 등량 흡착열에 관한 연구)

  • Lee, Jong Jib
    • Applied Chemistry for Engineering
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    • v.27 no.2
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    • pp.199-204
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    • 2016
  • The adsorption of Rhodamine-B dye using granular activated carbon from aqueous solution was investigated. Adsorption experiments were carried out as a function of the adsorbent dose, pH initial concentration, contact time and temperature. The equilibrium adsorption data showed a good fit to Langmuir isotherm model. Based on the estimated Langmuir separation factor ($R_L$ = 0.0164~0.0314), our adsorption process could be employed as an effective treatment method. The kinetics of adsorption followed the pseudo first order model. Also, the negative values of Gibbs free energy (-4.51~-13.44 kJ/mol) and positive enthalpy (128.97 kJ/mol) indicated that the adsorption was spontaneous and endothermic process. The isosteric heat of adsorption increased with increase in the surface loading indicating lateral interactions between the adsorbed dye molecules.

Adsorption Kinetic, Thermodynamic Parameter and Isosteric Heat for Adsorption of Crystal Violet by Activated Carbon (활성탄에 의한 Crystal Violet 흡착에 있어서 흡착동력학, 열역학 인자 및 등량흡착열)

  • Lee, Jong Jib
    • Applied Chemistry for Engineering
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    • v.28 no.2
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    • pp.206-213
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    • 2017
  • The adsorption of crystal violet dyes from aqueous solution using the granular activated carbon was investigated. Adsorption experiments were carried out as a function of the adsorbent dose, initial concentration, contact time and temperature. The adsorption characteristic of crystal violet followed Langmuir isotherm. Based on the estimated Langmuir separation factor ($R_L=0.02{\sim}0.106$), this process could be employed as an effective treatment (0 < $R_L$ < 1). The adsorption kinetics followed the pseudo second order model. The values of Gibbs free energy (-1.61~-11.66 kJ/mol) and positive enthalpy (147.209 kJ/mol) indicated that the adsorption process is a spontaneous and endothermic reaction. The isosteric heat of adsorption decreased with increasing of surface loading by the limited adsorbent-adsorbate interaction due to increased surface coverage.

An Experimental Study on Performance of the Fixed-type OWC Chamber for Wave-Energy Conversion (고정식 파력발전용 OWC챔버의 성능파악을 위한 실험적 연구)

  • B.S. Hyun;P.M. Lee;D.S. Gong
    • Journal of the Society of Naval Architects of Korea
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    • v.28 no.2
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    • pp.318-328
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    • 1991
  • The present paper describes the experimental study on the fixed-type wave-energy conversion system, consisting of the OWC-type wave-energy absorbing chamber and the duct for the air turbine. For simplicity, a screen of wire mesh was employed in place of an air turbine in order to simulate its effects on OWC chamber. Experiments were performed at the towing tank in regular waves with the frequency range of 0.22-0.75Hz. Comparison wish the numerical prediction using a potential flow-based method [4] was made to validate the capability of numerical code. It was shown that the agreements between measured and calculated results are quite good, giving a confidence in prediction method. Simulation of air turbine using a wire-mesh screen was successful, at least in a qualitative sense, to investigate the inter action between the OWC chamber and an air turbine. Results also showed that the effects of a wire-mesh screen on chamber efficiency are negligible, and the present model can be effectively utilized for the practical use in ocean waves with the frequency range under 0.3Hz.

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