• Annals of Clinical Microbiology
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• v.21 no.4
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• pp.86-91
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• 2018

Background: The PANA RealTyper HPV kit (PANAGENE, Korea; PANA RealTyper) was developed to genotype human papillomavirus (HPV) and was based on multiplex real-time PCR amplification and melting curve analysis. In this study, we compared PANA RealTyper to the AdvanSure HPV GenoBlot assay (LG Life Sciences, Korea; AdvanSure assay) and attempted to evaluate the performance of PANA RealTyper. Methods: A total of 60 cervical specimens were collected from women undergoing routine cervical cancer screening. The AdvanSure assay and PANA RealTyper kit identified the same 20 high-risk genotypes. However, the AdvanSure assay identified 15 low-risk genotypes, while the PANA RealTyper kit identified only 2 but detected 18 low-risk genotypes. Results: Among the total 60 specimens, 54 high-risk genotypes (40 specimens) and 20 low-risk genotypes (18 specimens) were detected. The agreement rates of the assays ranged from 94.4 to 100% for high-risk genotypes. Among 9 genotypes that were positive in the PANA RealTyper kit but negative in the AdvanSure assay, 7 were confirmed as true positive (HPV genotypes 16 (n=1), 39 (n=1), 52 (n=1), 58 (n=2), 68 (n=2)). Among 4 genotypes that were negative in the PANA RealTyper kit but positive in the AdvanSure assay, 3 were confirmed as HPV genotype 59. Among the 19 low-risk genotypes positive in the AdvanSure assay, there were 2 cases of HPV 6 and 1 case of HPV 11. In comparison, only 1 positive case of HPV 6 was determined by the PANA RealTyper kit. Conclusion: The PANA RealTyper kit was comparable with the AdvanSure assay. The PANA RealTyper kit would be useful and suitable for HPV genotyping in the clinical laboratory.

• Journal of Korean Library and Information Science Society
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• v.31 no.4
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• pp.49-64
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• 2000

본고는 무구정광대타라니경에 사용된 무구신자의 성격을 구명하기 위해 무주신자와 이를 제정한 측천무후에 대해 선행연구를 통해 살펴보고, 무주시대에 적용된 무주신자의 용례를 고찰하여, 본경에 사용된 례와 비교 분석한 것이다. 먼저 본경에 사용된 신자의 자형을 무주시기에 사용된 신자의 자형과 비교를 해본 결과, "초.증.수"자의 신자들은 상당한 차이가 났다. 또한 신자의 조합원리와 의미상에서 비교해 본 결과 더욱 차이가 있었다. 다음으로 신자사용의 적용례를 비교해 본 결과 역시 비교가 안될 정도의 차이를 보여주었다. 따라서 본고에서는 본경에 사용된 신자와 무주시기에 사용된 신자사이에 시간적.공간적으로 상당한 거리가 있기 때문이 이와 같이 자형과 사용례에서 극심한 차이가 있는 것으로 이해하였다.것으로 이해하였다.

• Proceedings of the Korea Water Resources Association Conference
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• 2004.05b
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• pp.468-473
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• 2004

본 연구에서는 기존의 ㅡ자형, ㄴ자형, ㄷ자형 조파시스템이 경사진 방향으로 가동될 때 발생되는 에너지의 불연속성으로 인한 회절철상이나 구조물에서 반사된 파가 조파판에서 발생하는 재 반사 철상의 단점을 보완할 수 있도록 곡선형태의 조파판을 배치하여 수치실험을 행하였다. 조파판을 곡선형으로 배치시켜 실험 대상물에서부터 반사된 파가 가능한 조파판에 직각으로 부딪치도록 유도하였다. 편미분 격자생성기법을 도입하여 구성된 곡선형 격자망에서 Copeland(1985)의 완경사 방정식을 이용하여 수치해석 하였다. 우선, 1차원 실험영역에서 파를 생성하여 대상구조물에서 반사되어 돌아온 반사파 성분을 조파판에서 흡수하면서 입사파를 생성하는 현상을 재현하였다. 그 후, 실험영역을 2차원으로 확장시켜 조파판을 곡선형으로 배치하여 수치 실험하여 진행파와 회절파의 중첩으로 고안된 해석해와 수치 해를 비교하려다. 그 결과, 기존의 조파시스템에 비하여 에너지 불연속선에서의 회절 현상도 발생하지 않고 반사파 성분을 효율적으로 흡수하는데 효과적임을 알아냈다.

• Journal of the Korean Society for information Management
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• v.10 no.2
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• pp.69-85
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• 1993

The purpose of this papel 1s to forecast the llbra~y as an ~ntelligent library and the librarian as a knowledgeeng~neer's type librar~an In the coming '"1st century, and then analyze the [ole and function of the library andIlbrarian. In order to make an intelhgent lib~ary and educate a knowledge eng~neer's type librar~an. the paper 1smade a study of the preparation to recelve the role and funct~on of knowledge engineering and knowledge englneerand IS presented plans for accepting it into the l~hralv and ~ntormation science.tion science.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.15 no.10 s.89
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• pp.938-943
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• 2004

In this paper, we propose a T-shaped DGS for CPW and provide the closed-form solutions for power loss rate, and bandwidth of the DGS. The proposed T-shaped DGS structure has a range of capacitance(C) 5.5 times wider than the dumbbell-shaped DGS structure. In addition we also analyze relations between R, L, C values of the DGS equivalent circuit and total loss rate, BW, $\omega_0$ of the DGS.

• Journal of Life Science
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• v.27 no.3
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• pp.295-300
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• 2017

This study examined the association between genotypes of the isocitrate dehydrogenase 3, beta subunit (IDH3B) gene and economic traits in an $F_2$ population of Duroc and Jeju (South Korea) native black pigs (JBPs). The genotypes was determined the presence/absence of a 304-bp insertion/deletion fragment in the promoter region of the IDH3B gene for JBP, Duroc, and their $F_1$ and $F_2$ progeny. Three genotypes (AA, AB and BB) were found in the $F_1$ and $F_2$ populations, but there was no AA genotype found in JBP and no BB in Duroc. Association analysis results showed the significant differences with carcass weights (CW), backfat thicknesses (BFT) and eye muscle area (EMA) (p<0.05), but not with growth traits including body weights and average daily gains at different stages, reproductive traits including teat numbers, and crude fat contents (CFAT) measured in longissimus dorsi (p>0.05). The $F_2$ pigs possessing the IDH3B BB homozygote had heavier CW ($72.92{\pm}11.133kg$), thicker BFT ($25.75{\pm}6.06mm$), and larger EMA ($23.82{\pm}4.825cm^2$) than those from the other genotypes (p<0.05). These results were estimated that there are biological roles related with IDH3B genotypes resulting development of EMA, BFT, and CW but not with intramuscular fat deposition during late period of pig production. Our findings suggest that the 304-bp insertion allele of porcine IDH3B may be a genetic marker for marker assistant selection for improving meat productivity of the Jeju Black pig and Duroc-related molecular breeding systems.

• Childhood Kidney Diseases
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• v.7 no.1
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• pp.52-59
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• 2003

Purpose : The renin-angiotensin system(RAS) plays an important role in renal growth and development. We have studied the prevalence of renal anomalies and documented the association between karyotype and renal anomalies using IVP and ultrasonography. Furthermore, to investigate the impact of RAS gene polymorphism on renal anomaly in Turner syndrome, we examined the ACE I/D genotype, angiotensinogen(AGT) gene M235T, angiotensin receptor type 1(ATR) gene A1166C. Methods : Cytogenetic analysis was performed in 33 Turner syndrome patients on peripheral blood lymphocytes. Ultrasonography(US) of the kidneys and collecting system and intravenous pyelography(IVP) were perfomed in all patients. Nuclear scintigraphy{Tc 99m dimercaptosuccinic acid(DMSA) scan} was also performed for the definite renal diagnosis if indicated. And, ACE I/D genotype, angiotensinogen(AGT) gene M235T, angiotensin receptor type 1(ATR) gene A1166C were examined by PCR amplification of genomic DNA samples. Results : The prevalence of renal anolmalies in Turner syndrome was 36.4%(12/33). The Karyotype 45, X was observed in 18 of the 33 girls(54.5%), of whom 8(44.4%) had renal anomalies. Mosaic karyotypes were observed in 11(33.3%) and four(12.2%) had a non-mosaic structural aberration of the X chromosome. In this group 4(25.7%) had renal anomalies. More renal anomalies were associated with the 45, X karyotype than those with mosaic/structural abnormalities of X chromosome, but the difference was not statistically significant(P>0.05). And, there was no significant differences in the RAS gene polymorphism and allele frequencies between renal anomaly group and normal group in Turner syndrome. Conclusion : The prevalence of renal anolmalies in Turner syndrome was 36.4%. There is no significant differences in the RAS gene polymorphism and allele frequencies between the renal anomaly group and the normal group in Turner syndrome.

• Journal of Life Science
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• v.21 no.4
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• pp.494-501
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• 2011

To understand the relationship between coat color inheritance patterns and genotypes of Extension (E) and Agouti (A) loci in cattle, the genotypes for melanocortin-1 receptor (MC1R) and agouti signaling protein (ASIP) were analyzed in Hanwoo, Jeju black cattle (JBC), and their crossbred progeny. Three MC1R alleles ($E^D$, $E^+$, and e) were found in the black-colored JBC population. JBC had no recessive homozygotes (e/e), but this genotype was predominant in the Hanwoo breed. However, MC1R $E^+$/e Hanwoo did not produce a black coat color as they appeared either as brown or solid red. For ASIP, three genotypes (A/A, A/$A^{Br}$, and $A^{Br}/A^{Br}$) were determined by insertion/deletion of an L1-BT element in Hanwoo. The ASIP $A^{Br}$ allele was rarely observed, and no ASIP $A^{Br}/A^{Br}$ homozygotes were detected in the JBC population. Cattle carrying ASIP $A^{Br}$ did not show any agouti-like brindle pigmentation patterns in either breed or their progeny. The coat colors of the crossbred progeny were discriminated by two colors, yellowish-brown versus dark-brown or black, and their coat colors were directly related to the genotypes of the Extension locus, yellowish-brown (e/e) and dark-brown or black ($E^+$/e), but not to the Agouti locus. ASIP genotypes probably did not affect coat color development in the Hanwoo or crossbred progeny. Our results suggest that the ASIP genotypes do not play key roles in coat color variation, but the MC1R genotypes do direct the phenotypes of Hanwoo, JBC, and their progeny.

• Korean Journal of Clinical Laboratory Science
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• v.48 no.3
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• pp.247-254
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• 2016

Human papillomavirus (HPV) infection is a necessary precondition of cervical cancer. A change from cytology to molecular HPV testing is, however, challenging. A new HPV DNA chip test for the infection of 22 HPV genotypes were developed in Korea. The purpose of this study was to investigate the prevalence and genotype distribution of HPV infection in the Seoul area. Over the last year, a total of 5,614 samples were tested. Using a chip test, HPV genotypes were detected in 1,596 (28.4%); of which, 679 (42.5%) were considered as high risk and low risk HPV were 152 (9.5%). 831 were single positive samples (n=1596). The most frequently found genotypes in all HPV-single positive samples (n=831) were HPV-16 (16.5%), 58 (15.2%), 52 (8.8%), 51 (7.1%) and 56 (5.9%). Mixed genotypes (n=219) were detected in 2 (n=176, 11.0%), 3 (n=37, 5.9%), and 4 (n=2, 0.1%) positive samples (n=1596). This study demonstrated that epidemical investigated HPV infection in patients of general hospital. These findings could be used to indicate a nationwide distribution of HPV and the adoption of vaccines. It is hoped that additional epidemiological research regarding the outcomes that are important to decision makers will be conducted.

• Horticultural Science & Technology
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• v.30 no.3
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• pp.278-285
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• 2012

Cymbidium goeringii is one of the most important and popular species in the orchid family in north-east Asia. In the present study, we prepared multiplex PCR system, and used it for the genotyping of eight simple sequence repeats (SSR) markers (CG409, CG415, CG709, CG722, CG787, CG1023, CG1210, and CG1281) in subject with 40 samples of cultivated varieties. All the analyzed samples showed different combined genotypes. The average combined power of discrimination was very high value of $7.14{\times}10^{-10}$, and observed heterozygosity (Ho = 0.466) was similar with two wild populations of C. goeringii, which may indicate no or little occurrence of genetic change after collection from wild habitats. The present study also developed a two-dimensional barcode to express information of genotype results of eight SSR markers (SSR DNA ID). The discrimination power of DNA ID between two individuals will be statistically more than 99.999999%. The SSR DNA ID and two-dimensional barcode may be very usefully applied for the discrimination and maintence of cultivars of C. goeringii.