• Polymer(Korea)
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• v.31 no.1
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• pp.14-19
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• 2007

Demineralized bone particle (DBP) has been used as one of the powerful inducers of bone and cartilage tissue specialization. In this study, we fabricated DBP/PLGA scaffold for tissue engineered disc regeneration. We manufactured dual-structured scaffold to compose inner cylinder and outer doughnut similar to nature disc tissue. The DBP/PLGA scaffold was characterized by porosity, wettability, and water uptake ability. We isolated and cultured nucleus pulposus (NP) and annulus fibrosus (AF) cells from rabbit intervertebral disc. We seeded NP cells into the inner core of the hybrid scaffold and AF cells into the outer portion of it. Cellular viability and proliferation were assayed by 3-(4,5-dimethylthiazole-2-yl) -2,5- diphenyltetrazolium -bromide (MTT) test. PLGA and PLGA/DBP scaffolds were implanted in subcutaneous of athymic nude mouse to observe the formation of disc-like tissue in vivo. And then we observed change of morphology and hematoxylin and eosin (H&E). Formation of disc-like tissue was better DBP/PLGA hybrid scaffold than control. Specially, we confirmed that scaffold impregnated 20 and 40% DBP affected to proliferation of disc cell and formation of disc-like tissue.

• The Journal of the Korean bone and joint tumor society
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• v.5 no.1
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• pp.9-16
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• 1999

A new method of limb sparing by resection, extracorporeal irradiation and reimplantation has several theoretical advantages. This method preserves the mobility of a joint and avoids the problem of loosening or breakage of tumor prosthesis. This study involved using extracorporeal irradiated autogenous joint transplantation for reconstruction after en bloc resection, and observed the periods of functional union and histological changes in irradiated tissue of the knee joint. This study also aimed to clarify whether the degeneration of articular cartilage is induced in rabbits by a single 50Gy dose of irradiation at the knee joint. Twenty New Zealand rabbits about three kilograms were randomized into two groups of 10 rabbits each. In group 1, as control, we resected the knee joint followed by reimplantation without irradiation. Group 2 received extracorporeal irradiation on the resected knee joint followed by reimplantation. Following are the results of these observations. The osteotomy site showed external callus formation in the roentgenographic finding eight weeks after reimplantation. There was marked degenerative changes in the collagen fiber of the irradiated anterior cruciate ligament and meniscus during the fourth week, but new blood-vessel formation was observed in the vicinity. There was degenerative changes in the collagen fiber of articular cartilage treated extracorporeal irradiation at four and eight weeks in the scanning electron micrographic findings. These findings was in contrast to those of subchondral bone which showed decreased cellularity and empty lacuna at four and eight weeks. Autoradiography demonstrated active [$^3H$]uridine incorporation by irradiated chondrocyte at eight weeks after reimplantation. These results indicate that when destruction of the articular cartilage and soft tissue of the knee joint is not severe, extracorporeal irradiation and reimplantation can be used with several advantage in maintaining movement of the joint while avoiding problems of tumor prosthesis and rejection, and therefore extracorporeal irradiated autogenous joint transplantation can be used as a limb-sparing procedure for temporary biological spacer in the childhood bone tumor around the knee.

• The Korean Journal of Mycology
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• v.14 no.2
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• pp.149-163
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• 1986

To determine contents of general constituents of Ganoderma lucidum in Korea, the dried carpophores were analyzed. The contents of water, ash, crude lipid, crude protein and crude cellulose were 14.6, 2.0, 3.3, 23.6, and 59.0%, respectively. Among reducing sugars, maltose was the most abundant. Seventeen free amino acids were detected, showing alanine the highest value. The pH of hot water extract was 4.1-4.2. The spores of Ganoderma lucidum was flat and ovoidal long. Their size was $6.3-7.1{\times}3.5-4.3{\times}2.0-2.5\;{\mu}m$ long. To examine effects of life span against sarcoma-180 cells, Fractions A, B and C, were obtained from the extract of Ganoderma lucidum. The survival rates of Fractions A, B and C were 131.7, 162.5, and 141.7 %, respectively. In addition, to examine effects of Fraction B on cell-mediated immunity, delayed type hypersensitivity (=DTH) test was conducted. It restored the suppressed DTH in the sarcoma-180 bearing group up to 66.7%.

• Journal of the Korean Society of Food Science and Nutrition
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• v.22 no.4
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• pp.405-411
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• 1993

Several studies have shown that phytol isolated from perilla leaf and small water dropwort (Oenanthe javanica (BL) D.C., wild type) extract reveal antirumor activities. In the present study we investigated the effect of phytol and the small water dropwort extract on the T subset in the sarcoma 180-transplanted mice in order to elucidate the immunological mechanism of antiturmor activity of these substances. The results obtained from the experiment were as follows : When phytol was injected into the sarcoma-180 transplanted mice (control), the levels of T cell and T subset by inoculation of the tumor cells were enhanced, but that of T cells in case of small water dropwort extract was similar to the control. Asialo GM1$^{＋}$ cells were increased when phytol or small water dropwort extract with injected into tumor-transplanted mice. In normal mice the number of asialo GM1$^{＋}$ cells increased with phytol injection and decreased with small water dropwort extract injection. L3T4$^{＋}$/Lyt-2$^{＋}$cell ratios were decreased when phytol was injected into tumor-transplanted mire, but increased in case of small water dropwort extract injection. In normal mice the ratios showed large decreases with phytol or small water dropwort extract injection. These results indicate that phytol or small water dropwort extract ran activate the proliferation of natural killer cells that are effector cells in tumor-bearing mice.

• The Journal of the Korean bone and joint tumor society
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• v.17 no.2
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• pp.65-72
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• 2011

Purpose: We evaluated the complications of allograft reconstruction after a bone tumor resection, and reviewed literatures to overcome such complications. Materials and Methods: We retrospectively reviewed clinical records and radiographs of fifteen patients in whom reconstruction with allograft after bone tumor resection. Results: Eight patients were men and seven were women with a mean age of 27.1 years (1-56 years) and a mean follow-up period of 89.5 months (33-165 months). All postoperative complications related to the allograft were recorded. Twenty patients (80.0%) obtained a radiologic bony union at a mean of 8.35 months (4-12 months). The mean Musculoskeletal Tumor Society score was 73.5% (46.6-93.0%). Nine patients (60.0%) experienced one event and 3 (20.0%) patients experienced multiple events during the follow-up period. Recorded events were infection (3), fracture (2), nonunion (2), limb length discrepancy (2) and varus deformity (2). The mean event free survival period was 60.8 months (6-144 months). The mean allograft survival period was 80.2 months and the 5 year survival rate of the allografts was 83.0%. Conclusion: In order to overcome complications, the combination of an allograft and vascularized fibular graft is highly recommended. In the near future, the tissue engineering technique, the application of the stem cell and PRP, could reduce the complication of allograft such as resorption and nonunion.

• Journal of Korea Multimedia Society
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• v.2 no.2
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• pp.217-229
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• 1999

Framework is the set of interrelated classes, constructing reusable design in specific domain or set of abstracted classes, and defines common architecture among applications included in domain. Developers can reuse not only class code but also wide range of knowledge on domain by reusing framework. In this papers, we present COM(Component-Oriented Methodology) for the reuse of framework, and develop construction environment for framework and domain development. That is, domain is analyzed by input of domain knowledge on real world to create software based on component, and hotspot is identified through analyzed information, and redesigned(refactoring) by putting additional information on users and developers. After that, I will create domain framework and application framework depending on domain. In this Component-oriented methodology, information is searched, understood and extracted or composite through component-pattern library storage internally. Then this information is classified into the information on component and pattern respectively, and used as additional information in redesigning. With this, developer can obtain reusability, easiness and portability by constructing infrastructure environment that allow to register, update and delete component through Component Pattern Management System(CPMS) under the development environment which can be easily applied to his own application using multimedia component, in this thesis, CORBA(Common Object Request Broker Architecture) environment.

• THE JOURNAL OF KOREAN ORIENTAL ONCOLOGY
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• v.3 no.1
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• pp.85-98
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• 1997

Oriental medicine as a candidate for effective cancer treatment recently gain positive concerns in fields of therapeutic oncology. that is why some herbal medicines have been empirically safer in toxicity than anticancer drugs used in western medicine, and to show excellent therapeutic efficacy in human trial. Thus, these effects by clinically applied-herbs have not yet fully demonstrated in experimental tumor model. This study was initiated to evaluate the antitumor effect of Insambaekhaptang as candidate of antitumor-herbal agent against B16 melanoma metastasized into C57BL/6 mice lung. In experiment to test whether Insambaekhaptang can directly kill cancer cells in vitro or not, Insambaekhaptang showed direct killing action in concentration or higher against B16 melanoma cells using MTT assay, and showed lower IC50. Another experiment to know whether Insambaekhaptang can inhibit growth and metastasis of cancer cell or not, Insambaekhaptang significantly inhibited Solid tumor by intraperiperal injected-melanoma and lung metastasis induced by intravenous injected-melanoma in inbred C57BL/6 mice. When quantitative survival time increasing, we could obtain results that increased 113% in treated by Insambaekhaptang. These results show that Insambaekhaptang can inhibit growth of B16 melanoma cells through various biological mechanisms.

• Journal of Biosystems Engineering
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• v.27 no.3
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• pp.211-218
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• 2002

배추 생산에 있어서 수확, 운반, 적재 작업은 가장 노동이 집약적으로 요구되는 작업들이다. 최근, 여러 종류의 양배추 수화기가 일본과 유럽에서 개발되었다. 하지만 국내에서 재래되는 배추는 크기와 형태에 있어 양배추와는 달라 기 개발 기종의 도입이 어렵다. 또한 수확작업의 생력화 효과는 운반, 정선, 적재 작업과 밀접하게 연계되어 있어 출하시의 작업체계를 고려하여 수화에 따른 수집.반출 시스템을 개발하여야 한다. 수확시의 배추는 중량이 25~45 N 정도로 다 작물에 비하여 무겁고 부피가 크기 때문에 수확작업의 기계화를 위해서는 수확장치와 더불어 연속적으로 수확되는 배추를 적절하게 수집하여 적재하고 반출하는 시스템의 개발이 중요하다. 본 연구에서는 수확작업의 생력화 효과를 높이고 생력화 시스템 비용의 절감을 목적으로 작업자 1인에 의해 작업할 수 있는 반자동 형태의 트랙터 부착형 배추 수집, 적재, 반출시스템 시작기를 개발하였다. 시작기는 배추 이송장치, 적재장치, 팔렛 및 반출장치의 3개 부분과 PLC를 이용한 주 제어기로 구성하였다. 배추 수집용기로는 대략 70개의 배추를 담을 수 있는 크기가 1,050 mm$\times$1.050mm$\times$1,000mm 인 접이식 메쉬 팔렛을 사용하였으며 하단부에 롤러 안내판을 부착하여 적재한 팔렛의 배출이 용이하도록 하였다. 팔렛을 제외한 전체 시작기의 중량은 235 N 이였으며 크기는 3,940mm$\times$520mm$\times$1,630mm 이었다. 본 연구는 수확장치의 기능 및 생력화 효과를 극대화하고 배추의 손상정도를 최소화하는 시스템을 구성하고자 하였다. 이송장치는 트랙터 부착시 횡공간 점유율을 최소화하도록 하였으며 적재장치는 적재시 배추의 손상을 줄이고 배추가 놓이는 자세를 능동적으로 조절할 수 있도록 주름관을 부착하였다. 시작기의 실내시험 결과 이송장치는 0.18 m/s~0.36 m/s의 범위에서 적재장치는 0.4 m/s~2.4 m/s 범위에서 안정적으로 구동하였으며 두 장치를 동시에 구동하여 시험한 결과 이송장치는 0.26 m/s~0.36 m/s, 그리고 적재장치는 0.9 m/s~2.4 m/s 에서 적정하게 안정적으로 구동하였다. 적재장치의 성능에 있어서 1~3단 적재시에는 주름관을 이용하여 적재하고 4~5단 적재시에는 자유낙하에 의한 적재를 수행할 경우 인력에 의한 적재와 거의 동등한 적재량을 보였으며 손상정도는 거의 무시할 정도였다. 트랙터가 0.3 m/s로 주행하는 경우 노지로부터 배추를 뽑아 이송하는 뽑기벨트의 적정속도가 0.46 m/s인 점을 고려할 때 배추 이송 컨베이어는 0.34 m/s 이상의 속도를 유지할 필요가 있었으며 적재 컨베이어는 2 m/s~2.4 m/s의 속도에서 안정적으로 작동하였다. 배추의 주간 거리가 대략 30~40 cm 인 점을 감안하면 적재장치는 초당 1개의 적재성능을 보였다. 실내에서 수행한 시스템의 성능은 배추에 큰 손상없이 전반적으로 성공적으로 구동하였으나 향후 노면이 고르지 못한 포장에서의 성능 시험이 필요하다.

• Korean Journal of Animal Reproduction
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• v.17 no.2
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• pp.165-171
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• 1993

While ornithine decarboxylase (ODC) is considered a key enzyme in the biosynthesis of polyamines, difluoromethylornithine(DFMO) acts as an inhibitor of polyamine synthesis. Cycling crossbred gilts were randomly assigned to one of two (treatment and control) groups (6/group). An indwelling silicone catheter was surgically implanted in the jugular vein of each animal. DFMO was dissolved in saline(200 mg/ml) and adminstered by i. m. injection at a dose of 80 mg/kg/day. The control group received an equivalent volume saline injection. DFMO was injected 3 times daily(08:00. 16:00. 24:00h) from day 16 of estrous cycle to 21 or until estrus. Once daily blood samples (10ml) were taken from day 14 until two days after the last DFMO treatment. Window blood samples were collected every 15 min for 8 h (from 08:00 to 16:00h) starting on day 16 and continuing until day 21 from one gilt per day. Serum progesterone (P$_4$), estradiol (E$_2$), LH and FSH were measured. Typical concentration profiles for P$_4$ and E$_2$ were seen during the follicular phase regardless of DFMO treatment. Injection of DFMO suppressed the preovulatory LH concentration in the serum(p<0.01) while having no effect on FSH profile. The present results indicate that DFMO had an inhibitory effect on LH secretion in the pig, but did not affect PI, E2 or FSH release.

• Journal of the Korean Arthroscopy Society
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• v.2 no.1
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• pp.51-58
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• 1998

Purpose : The purpose of this study was to compare the postoperative success and stability of arthroscopically assisted anterior cruciate ligament(ACL) reconstructions using the central one third bone patellar tendon bone(BPB) autograft versus a quadrupled semitendinosus/gracilis(ST) autograft in patients with "isolated" ACL tears. Materials & Methods : A strict criteria to identify isolated ACL tears was used which included : no previous surgery, no other ligamentous injury, no history of patellofemoral symptoms, no patellofemoral malalignment, no meniscal pathology, no chondromalacia or chondral injury and no limitation of motion of the injured knee. 30 patients (15 BPB, 15 ST) with a mean age of 27.4 years were available for a mean follow up of 18 months (between 12 months and 26 months). Preoperatively, there was no significant difference between the two groups with respect to age, sex and degree of laxity. Results : Postoperatively, we couldn't find significant differences between the two groups with respect to subjective Lysholm score, objective laxity including Lachman test, pivot shift test and KT-2000 measurements. Mean side to side difference of KT-2000 scores at 20lbs were 1.5mm for the BPB group and 1.4mm for the ST group. Positive Lachman test was found in 26.7% and 33.3% and positive pivot shift was found in 20% and 33.3% of the patients in the BPB and ST groups, respectively. Anterior knee pain (33.3%) was more common in the BPB group. There were 80% of the patients in both groups above nearly normal grade according to the IKDC grade. Conclusions : In patients with "isolated" ACL tears, the overall results, ligamentous stability for the patellar tendon and the quadrupled semitendinosus/gracilis were comparable. We consider that the quadrupled autogenous hamstring tendon is a good alternative substitute in ACL reconstruction together with the bone patellar tendon bone.