• Horticultural Science & Technology
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• v.33 no.4
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• pp.618-623
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• 2015

The new white calla lily (Zantedeschia aethiopica) cultivar 'White Cutie' was bred at the National Institute of Horticultural & Herbal Science (NIHHS) in 2011. 'Childsiana' showing the multi-flowering characteristic and 'Wedding March' resistant to soft rot disease were artificially crossed in 2004. Of the progeny, 'White Cutie' was selected specifically for use in cut flower production after investigation over seven years (2005 to 2011) of genetic and phenotypic characteristics, resistance against soft rot, and customer preference regarding the culture vigor and post-harvest quality. 'White Cutie' was early flowering (85.6 days to flowering) with white flowers (RHS W155C), although it had a mid-sized flower in which spathe height and width were 8.6 cm and 8.7 cm, respectively. It was multi-flowering (6.2 flowers per plant) and produced a very high number of cormels (13.4 per plant). Furthermore, it was resistant to soft rot disease.

• Research in Plant Disease
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• v.10 no.1
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• pp.78-81
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• 2004

The resistance of 10 varieties of cucumber (Cucumis sativus L.) to powdery mildew, caused by Sphaerotheca fusca, was evaluated by a leaf disk assay. Leaf disks (10 mm in diameter) were removed from fully expanded leaves and then placed in petri dishes containing 0.16％ water agar amended with benzimidazole. Leaf disks were inoculated by dropping a 10 $\mu$l of conidia suspension. Conidiophore formation of powdery mildew was the greatest at $25^{\circ}C$. The response of the host to powdery mildew, based on the inoculation onto disks of the first leaf, highly correlated with results obtained from harvesting stage of cucumber plants in greenhouse test (r = 0.99$^{**}$). It is indicating that a leaf disk assay may precisely predict the response of cucumber plant to S. fusca.a.

• Korean Journal of Plant Tissue Culture
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• v.28 no.5
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• pp.243-248
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• 2001

This study was peformed to find out the optimal conditions for the selection of transformed cells using already established transgenic plants. Several transgenic poplar (Populus alba$\times$P giandulosa) lines carrying npt II or hpt gene as a selectable marker were tested against kanamycin or hygromycin. Two culture explants, leaf discs and nodes, were compared regarding their sensitivity to the antibiotics. When leaf discs of untransformed control plants were cultured on callus inducing media in the presence of varying levels of kanamycin or hygromycin, only those cultured on the media containing lower than 50 mg/L kanamycin or 2 mg/L hygromycin formed callus. However, much higher concentration of kanamycin was needed to suppress the growth of axillary buds of untransformed plants. On the other hand, hygromycin at the concentration of 5 mg/L effectively suppressed shoot growth of untransformed plants. Root induction from untransformed plants could also be suppressed at the concentration of 50 mg/L kanamycin or 5 mg/L hygromycin. The transgenic plants showed resistance to 100 mg/L kanamycin or 50 mg/L hygromycin in the growth of callus, shoots, and roots. Hygromycin appeared to be more efficient in selecting untransformed cells than kanamycin.