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Disease Resistance Test Method of Cucumber Powdery Mildew(Sphaerotheca fusca) Using A Leaf Disk Assay

잎절편 (Leaf disk)을 이용한 오이 횐가루병 (Sphaerotheca fusca)에 대한 내병성 검정법

  • Published : 2004.03.01

Abstract

The resistance of 10 varieties of cucumber (Cucumis sativus L.) to powdery mildew, caused by Sphaerotheca fusca, was evaluated by a leaf disk assay. Leaf disks (10 mm in diameter) were removed from fully expanded leaves and then placed in petri dishes containing 0.16% water agar amended with benzimidazole. Leaf disks were inoculated by dropping a 10 $\mu$l of conidia suspension. Conidiophore formation of powdery mildew was the greatest at $25^{\circ}C$. The response of the host to powdery mildew, based on the inoculation onto disks of the first leaf, highly correlated with results obtained from harvesting stage of cucumber plants in greenhouse test (r = 0.99$^{**}$). It is indicating that a leaf disk assay may precisely predict the response of cucumber plant to S. fusca.a.

잎절편법을 이용하여 오이 흰가루병(Sphaerorheca fusca)에 대한 10개 품종의 저항성을 평가하였다 완전히 전개된 오이 잎에서 직경 10mm의 절편를 잘라내어 benzimidazole을 혼합한 한천배지에 올려놓고 포자현탁액을 10$\mu$l씩 절편에 점접종법으로 실험하였다. 흰가루병의 분생자경은 $25^{\circ}C$에서 가장 잘 형성되었다. 제1본엽의 disk를 이용하여 분생포자형성정도를 측정해 흰가루병에 대한 오이의 저항성을 평가한 결과와 하우스 포장에서의 발병도 결과간에 고도의 정의 상관을 보였다(r=0.99$^{**}$ ). 이 결과들을 통해 잎절편법을 이용하여 오이 식물체의 흰가루병(S. fusca)에 대한 저항성을 평가할 수 있었다.

Keywords

References

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